Surface-enhanced Raman scattering (SERS) technology faces reliability and reproducibility challenges in bio-related analysis, but Prussian blue (PB)-based SERS tags offer a potential solution due to their distinctive Raman band that allows for background-free detection.
The multi-layer structure of PB ensures stable signal output by preventing the desorption of Raman reporters, alongside possessing excellent multiplexing capabilities for analyzing multiple targets simultaneously.
This minireview emphasizes the current state and future prospects of PB-based SERS tags in various biological applications, including quantification, cell imaging, and their roles in clinical diagnosis and treatment.