Spontaneous and site-specific immobilization of PNGase F spy chemistry.

Protein -glycosylation plays a critical role in a wide range of biological processes, and aberrant -glycosylation is frequently associated with various pathological states. For global -glycosylation analysis, -glycans are typically released from glycoproteins mediated by endoglycosidases, primarily peptide -glycosidase F (PNGase F). However, conventional -glycan release by in-solution PNGase F is time-consuming and nonreusable.