Wu Mei Wan suppresses colorectal cancer stemness by regulating Sox9 expression via JAK2/STAT3 pathway.

Ethnopharmacological Relevance: Wu Mei Wan (WMW) is a traditional Chinese herbal formula with a long-standing history in Chinese medicine, valued for its therapeutic properties. However, its potential anti-cancer effects, especially against colorectal cancer (CRC), have not been fully elucidated.

Aim Of The Study: This study aims to investigate the effects of WMW on colorectal cancer stemness and to elucidate the underlying molecular mechanisms, focusing on the modulation of Sox9 expression via the JAK2/STAT3 signaling pathway.

Exploring and identifying the imaging biomarkers for predicting anti-VEGF treatment response in polypoidal choroidal vasculopathy: a prospective multicenter study.

Background: Polypoidal choroidal vasculopathy (PCV) is a hemorrhagic fundus disease that can lead to permanent vision loss. Predicting the treatment response to anti-VEGF monotherapy in PCV is consistently challenging. We aimed to conduct a prospective multicenter study to explore and identify the imaging biomarkers for predicting the anti-VEGF treatment response in PCV patients, establish predictive model, and undergo multicenter validation.

Changes in responses of neurons in spinal and medullary subnucleus reticularis dorsalis to acupoint stimulation in rats with visceral hyperalgesia.

The purpose of this study was to explore the mechanism of acupoints sensitization phenomenon at the spinal and medulla levels. Experiments were performed on adult male Sprague-Dawley rats and visceral noxious stimuli was generated by colorectal distension (CRD). The activities of wide dynamic range (WDR) and subnucleus reticularis dorsalis (SRD) neurons were recorded.

[Effect of electroacupuncture at fenglong (ST40) on expressions of inflammatory factors in macrophages of hyperlipidemia model rats].

Objective: To observe the effect of electroacupuncture (EA) at Fenglong (ST40) on inflammatory factors in macrophages of hyperlipemia (HLP) rats, and to explore the role of EA in treating HLP.

Methods: Totally 50 adult male SD rats were selected. After one-week adaptative feeding, they were randomly divided into 5 groups, i.

Associations between physical activity-related miRNAs and metabolic syndrome.

The aim of the study was to investigate the associations between physical activity (PA)-related miRNAs and metabolic syndrome (MetS). A case-control study was conducted in 209 subjects with MetS and 234 controls. The MetS was defined by the International Diabetes Foundation (IDF) criteria of 2005.

[Comparison of therapeutic effects of different types of acupuncture interventions on herpes zoster in acute stage].

Objective: To compare the therapeutic effects of different types of acupuncture therapy on herpes zoster (acute stage).

Methods: A total of 189 cases of acute herpes zoster outpatients and inpatients were recruited in the present multicenters (3 hospitals) randomized controlled clinical trials. They were divided into (computer-aided random allocation): basic acupuncture (n = 36), cotton moxibustion (n = 35), fire needle (n = 43), tapping-cupping (n = 39) and medication (n = 36) groups.

[Clinical observation of treating osteoporosis vertebral compression fracture of senile patients by restitution combined percutaneous vertebroplasty].

Objective: To explore the methods and therapeutic efficacy of restitution combined with percutaneous vertebroplasty (PVP) for treating osteoporosis vertebral compression fracture (OVCF).

Methods: Recruited were 132 senile patients with OVCF who were willing to receive minimally invasive therapy were assigned to the comprehensive treatment group and the percutaneous kyphoplasty (PKP) group. The 89 vertebral bodies in the 68 cases of the comprehensive treatment group received restitution combined with PVP, while the 81 vertebral bodies in the 64 cases of the control PKP group received PKP alone.

[Effect of electroacupuncture at "Fenglong" (ST 40) on rats with hyperlipidemia and its mechanism].

Objective: To explore the mechanism of electroacupuncture(EA) at "Fenglong" (ST 40) in hyperlipidemia (HLP) rats.

Methods: Forty health SD rats were randomly divided into a normal control group (group A), a high fat forage feed group (group B) and a high fat forage feed treatment group (group C), a high fat forage + normal forage feed group (group D) and a high fat forage + normal forage feed treatment group (group E), eight rats in each group. EA was applied at "Fenglong" (ST 40) for the rats in group C and group E, once daily.

[Effect of scalp-acupuncture on the proliferation and migration of neural stem cells in acute cerebral ischemia/reperfusion injury rats].

Objective: To study the proliferation and migration of neural stem cells (NSCs) of acute cerebral ischemia rats and the intervention of scalp-acupuncture (SA), and to study its action of mechanism in treating cerebral ischemia.

Methods: One hundred healthy Wistar rats were randomly divided into the sham-operation group (n = 10), the model group (n = 45), and the SA group (n = 45). The middle cerebral artery occlusion (MCAO) model was established using the modified suture method.

[Effects of Yiniao Recipe on serum antidiuretic hormone level and plasma ratio of cAMP to cGMP in rats with kidney-yang deficiency].

Objective: To investigate the effects of Yiniao Recipe, a compound traditional Chinese herbal medicine, on contents of serum antidiuretic hormone, and plasma cyclic adenosine monophosphate and cyclic guanosine monophosphate in rats with kidney-yang deficiency.

Methods: Forty male Wistar rats were randomly divided into blank control group, untreated group, desmopressin (Minirin) group, low-dose Yiniao Recipe group and high-dose Yiniao Recipe group, with 8 rats in each group. Rats in the blank control group were injected with 0.

[Observation on therapeutic effect of electroacupuncture at Jiaji (EX-B 2) combined with blood-letting and cupping on herpes zoster].

Objective: To compare the therapeutic effect differences between electroacupuncture at Jiaji (EX-B 2) combined with blood-letting plus cupping and western medicine therapy.

Methods: Fifty-three cases were randomly divided into an observation group (n=31) and a control group (n=22). The observation group was treated by electroacupuncture at Jiaji (EX-B 2) combined with blood-letting with a plum-blossom needle at the affected parts plus cupping, once each day.

[Observation on therapeutic effect of electroacupuncture combined with acupoint-injection on acute gouty arthritis].

Objective: To search for a new method for treatment of acute gouty arthritis.

Methods: Sixty cases of acute gouty arthritis were randomly divided into a treatment group and a control group, 30 cases in each group. The treatment group were treated with electroacupuncture combined with point-injection, and the control group with western medicine.