[Effects of microRNA-21 on the myocardial cell apoptosis induced by ischemia and hypoxia in rat].

Objective: To explore the effects of microRNA-21 on apoptosis of myocardial cell of rats as induced by ischemia and hypoxia, and to analyze the underlying mechanisms.

Methods: (1) Rat myocardial cell line H9C2 was cultured in a serum-free and low glucose DMEM medium using a hypoxic incubator which was filled with 1% oxygen, 5% carbon dioxide, and 94% nitrogen to simulate ischemic environment. The expression of microRNA-21 in normal myocardial cells and cells treated with low oxygen exposure for 6 and 24 h were assessed by real-time fluorescent quantitative RT-PCR.

[Effects of low molecular weight heparin on the inflammatory response and vascular injury in rat after electric burn].

Objective: To observe the effects of low molecular weight heparin (LMWH) on the inflammatory response and vascular injury in rat after electric burn.

Methods: A homemade regulator and transformer apparatus was used to reproduce the model of electric burn (0.5 cm×0.

Enhanced activity of doxorubicin in drug resistant A549 tumor cells by encapsulation of P-glycoprotein inhibitor in PLGA-based nanovectors.

Effective chemotherapy remains an important issue in the treatment of drug resistant cancer. The aim of the present study was to establish novel polymeric nanoparticles composed of the antitumor drug, doxorubicin (DOX), and an inhibitor of the drug efflux pump-associated protein, P-glycoprotein (P-gp), in order to overcome drug resistance in tumor cells. Poly(D,L-lactide--glycolide) (PLGA), DOX-loaded PLGA (PLGA-DOX), P-gp inhibitor (cyclosporin A; CsA)-coated PLGA (PLGA-CsA) and DOX and CsA co-loaded PLGA (PLGA-DOX-CsA) nanoparticles were prepared using solvent evaporation.

[Treatment of thoracic and abdominal cavity perforation complicated by Henoch-Schonlein purpura nephritis in a patient with high-voltage electric burn].

A 55-year-old male patient suffered from severe high-voltage electric burn with an area of 20%TBSA full-thickness injury. The injury involved the distal end of left upper limb, right trunk, and whole abdominal wall. Fracture of the 7th-10th ribs was found in the right side of chest, with perforation of abdominal cavity, and bilateral pleural effusion was found.

[An overview on the establishment of the system of burn prevention and treatment in China by comparing with that of foreign countries].

The number of burn patients in China is still huge because of the demographic and socio-economical reasons. Distinguished achievements have been made in Chinese burn surgery during decades of development, with a generally acknowledged high survival rate of burn victims. However, there are still many problems, including an emphasis on the treatment rather than on prevention, poor pre-hospital care system, unbalanced development of burn centers, shortage of burn rehabilitation and psycho-social support, shortage of financial support, and underdevelopment of social charity for burn patients.

[Role of biological protective dressing in microskin grafting in rabbit].

Objective: To study the effect of biological protective dressing made from porcine peritoneum in covering wounds with microskin grafts.

Methods: Twenty New Zealand rabbits were divided into ten couples according to the random number table. Rabbits in each couple underwent surgery at the same time.

Childhood sexual abuse and the risk for recurrent major depression in Chinese women.

Background: Studies in Western countries have repeatedly shown that women with a history of childhood sexual abuse (CSA) are at increased risk for developing major depression (MD). Would this relationship be found in China?

Method: Three levels of CSA (non-genital, genital, and intercourse) were assessed by self-report in two groups of Han Chinese women: 1970 clinically ascertained with recurrent MD and 2597 matched controls. Diagnostic and other risk factor information was assessed at personal interview.

[Retrospective investigation of inpatients with chronic wounds].

Objective: To investigate cause of chronic wounds and related status of patients so as to provide strategy for study and treatment of chronic wounds and establishment of health policy.

Methods: A total of twelve thousand one hundred and sixty-one cases hospitalized in our hospital in 2008 were enrolled in the study. A chronic wound was defined as skin tissue defect which could not heal after one month of treatment.

[Treatment of facial pigmentation after burns with traditional Chinese medicine mask and skin care].

Objective: To observe the effects of traditional Chinese medicine mask combined with skin care in the treatment of facial pigmentation after burns.

Methods: Forty-one patients with facial pigmentation after burns hospitalized from January 2009 to June 2010 were enrolled and divided into treatment group (n = 26, odd number) and control group (n = 15, even number) according to the visiting order. After cleaning, massaging, and steam spraying to faces, patients in treatment and control groups were respectively treated with traditional Chinese medicine mask developed by physicians in our hospital and common commercial beauty mask.

[Burn rehabilitation and community reintegration-new challenge to burn surgery in China].

Burn patients often have severe disfigurement, dysfunction, and psychological disorder after discharge, which may last for a long time, even for a whole life. These problems may prevent patients from returning to normal life and re-entering society. Because of demographic and socioeconomic reasons, the number of burn patients in China is huge.

[Distribution and vicissitude of drug resistance of Acinetobacter baumannii in burn intensive care unit of Wuhan City Hospital No.3 in three years].

Objective: To study the clinical distribution characteristics and vicissitude of antibiotic resistance of Acinetobacter baumannii (AB), and to look for the risk factors of AB infection in order to provide reasonable reference for the prevention and treatment of its infection.

Methods: Specimens of blood, venous catheters, sputum, wound exudates and pharyngeal swabs from 156 patients hospitalized in our burn ICU from January 2006 to December 2008 were collected and cultured. The clinical distribution and antibiotic resistance of AB were determined and analyzed.

[Diagnosis of the deep partial-thickness burn wound of Skh-1 mouse with Optical Coherence Tomography].

Objective: To evaluate the application value of Optical Coherence Tomography (OCT) in the diagnosis of the depth of burn wound.

Methods: Deep partial-thickness scald models of Skh-1 mice were reproduced using self-made steam scald appliance. The scald wounds were scanned with OCT 3 hours, or 3 and 8 days after injury respectively.

[Repair of electrical burns on hands with flaps].

Objective: To summarize the experiences and shortcomings of repair of wounds on hands due to electrical burns with flaps, aiming at further improvement.

Methods: Clinical data of 425 patients with electrical burn of hands admitted to Burn Institute of Wuhan, City Hospital NO. 3 & Tongren Hospital of Wuhan University from January 2000 to December 2006 were collected and summarized.