[Cloning and function characterization of a UDP-rhamnose synthase from Dendrobium huoshanense].

UDP-rhamnose, which is synthesized under the catalysis by the rhamnose synthase(RHM), is an essential sugar donor for the synthesis of rhamnoside in plants. Based on the reported rhamnose synthase, this study screened one RHM gene(DhuRHM) from the localized gene database of Dendrobium huoshanense by sequence alignment. This gene was cloned, and then bioinformatics analysis and in vitro functional verification were carried out.

[Cloning and functional analysis of flavanone synthase Ⅱ from Dendrobium huoshanense].

Flavonoid C-glycosides are a class of natural products that are widely involved in plant defense responses and have diverse pharmacological activities. They are also important active ingredients of Dendrobium huoshanense. Flavanone synthase Ⅱ has been proven to be a key enzyme in the synthesis pathway of flavonoid C-glycosides in plants, and their catalytic product 2-hydroxyflavanone is the precursor compound for the synthesis of various reported flavonoid C-glycosides.

Highly efficient Fe-Cu dual-site nanoparticles supported on black pearls 2000 (carbon black) as oxygen reduction reaction catalysts for Al-air batteries.

Acquiring cost-effective, high-performance, non-precious metal catalysts is crucial for substituting precious metal catalysts in the oxygen reduction reaction (ORR) to ensure sustainable energy conversion. Herein, we present a preparation strategy for a high-performance Cu-Fe-CN-3 electrocatalyst characterized X-ray diffraction (XRD), Raman spectroscopy, Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS) analyses. The results demonstrated that the incorporation of Cu and Fe into Black Pearls' carbon black (BP2000) and the strong synergistic effect between Fe and Cu contributed to the enhancement of active sites for the ORR.

The preparation and characterization of self-healing hydrogels based on polypeptides with a dual response to light and hydrogen peroxide.

Injectable self-healing hydrogels are being widely used in drug delivery, tissue engineering, and other fields. Because of their excellent biocompatibility and biodegradability, polypeptides are an ideal candidate for preparing injectable self-healing hydrogels. In this study, a polypeptide-based hydrogel with dual response to hydrogen peroxide and light was obtained by copolymerizing 4-arm PEG-amine, -(-nitrophenoxycarbonyl)-l-methionine, and -(-nitrophenoxycarbonyl)-γ--nitrobenzyl-l-glutamate.

[Comparison of planting modes of Dendrobium huoshanense and analysis of advantages of simulated cultivation].

Dendrobium huoshanense is a precious medicinal plant belonging to Dendrobium of Orchidaceae. It is a special medicinal material and extremely scarce in Huoshan county, Anhui province. At present, D.

[Ecological agriculture of Chinese materia medica:update and future perspectives].

This study steps through four key principles, four open problems and future perspectives of Chinese materia medica(CMM) ecological agriculture by presenting the historical development, existing theories and practice outcomes. Then, it focuses on refining the main principles of CMM ecological agriculture:(1)the principles of ecological niche associated with yield and comprehensive income;(2)principles of biological diversity associated with the integrated control of diseases, pests and weeds;(3)principles of adversity effects associated with the quality improvement of CMM;(4)principles of structural stability associated with the sustainable development of CMM ecological agriculture. On this basis, four burning issues of CMM ecological agriculture were obtained,(1)ecological planting mode and supporting technologies need to be perfect;(2)multi-integrated industrial coupling remains to strengthen;(3) quality assurance system of CMM ecological agriculture and high-quality and favorable price model remains to be formed;(4)awareness of the demonstration and extension of CMM ecological agriculture needs to be desired.

[Comparison of active ingredients and protective effects of Dendrobium huoshanense of different growth years on acute liver injury].

The differences of the active ingredients in Dendrobium huoshanense of different growth years and their protective effects on acute liver injury were studied to provide evidence for optimizing harvest time. The contents of polysaccharides, total flavonoids and total alkaloids in D. huoshanense of different growth years were determined by UV spectrophotometry, and the contents of gigantol in D.

[Strategies for medicinal plants adapting environmental stress and "simulative habitat cultivation" of Dao-di herbs].

This paper analyzed life form, habitats and environmental stresses of medicinal plants and algal fungi collected in Chinese Pharmacopoeia(2015). ①It was found that only 0.94% of the medicinal plants mainly cultivated in field.

[Diversity and difference of endophytes in Dendrobium huoshanense with different growth years].

In order to explore endophytes diversity and difference in Dendrobium huoshanense,in this paper,the metagenomics method was used to analyze the endophytic bacteria and fungi community of 5 groups include 30 samples in different growth years. The results indicate that 3 540 bacterial OTUs were identified from D. huoshanense,and there are 138 OTUs in 5 groups simultaneously;2 168 fungal OTUs were identified,and 143 OTUs exist in 5 groups simultaneously.