Prediction of OCT contours of short-term response to anti-VEGF treatment for diabetic macular edema using generative adversarial networks.

Diabetic macular edema (DME) stands as a leading cause for vision loss among the working-age population. Anti-vascular endothelial growth factor (VEGF) agents are currently recognized as the first-line treatment. However, a significant portion of patients remain insensitive to anti-VEGF, resulting in sustained visual impairment.

MEF2C mitigates coronary artery lesions in Kawasaki disease by enhancing endothelial barrier function through KLF2 regulation.

Coronary artery lesions constitute a significant complication of Kawasaki disease (KD) and represents one of the primary etiologies of acquired cardiovascular disease in pediatric populations. In the present study, we observed a downregulation of MEF2C expression in the whole blood of KD patients and in human coronary artery endothelial cells (HCAECs) during the pathophysiological progression of KD. Furthermore, transcriptomic data analysis, in conjunction with observations from HCAECs stimulated with KD serum, indicates that the downregulation of MEF2C in KD is correlated with increased inflammatory levels and the activation of inflammatory pathways.

CD271 regulates osteogenic differentiation of ectomesenchymal stem cells via the RhoA/ROCK signaling pathway.

The low-affinity neurotrophic receptor CD271 plays a crucial role in the osteogenic differentiation of ectomesenchyme stem cells (EMSCs), which is essential for the development and regeneration of jaw bones. This study aimed to investigate the influence of CD271 on EMSCs osteogenic differentiation and to uncover the underlying mechanisms. CD271-deficient mice exhibited delayed mandibular bone development, with a significantly reduction in the expression of osteogenic makers such as ALP, Col-1, OPN, and RUNX2.

Application of infrared thermography for predicting pressure injury healing: A prospective study.

Background: An accurate assessment of pressure injury healing is crucial for the timely implementation of nursing intervention. This study aimed to investigate the accuracy of infrared thermography-based wound temperature measurement in predicting wound outcomes.

Methods: This prospective, 16 days observational study included 156 adults with pressure injury.

Associations of blood selenium with dyslipidemia in children and adolescents: a cross-sectional analysis.

Objective: This study aims to examine the association between blood selenium levels and dyslipidemia in children and adolescents.

Methods: Data from the National Health and Nutrition Examination Survey (NHANES) 2011-2020 were analyzed, including 8191 participants aged 6-19 years. Dyslipidemia was defined by elevated total cholesterol (TC ≥ 200 mg/dL), lowered high-density lipoprotein cholesterol (HDL-C < 40 mg/dL), or elevated non-HDL-C (≥ 145 mg/dL).

Stochastic galvanic vestibular stimulation improves kinetic performance in adolescent idiopathic scoliosis during obstacle negotiation.

Vestibular dysfunction has been reported as a potential cause in adolescent idiopathic scoliosis (AIS). However, it remained unclear how stochastic galvanic vestibular stimulation (GVS) affected kinetic performance of patients with AIS. This study aimed to investigate the effect of stochastic GVS on ground reaction forces (GRF) measures during obstacle negotiation among patients with AIS.

Sodium tanshinone IIA sulfonate alleviates fetal growth restriction by mediating aquaporin-3 expression in placental trophoblast cells.

Fetal growth restriction (FGR) is characterized by the inability of the fetus to achieve its growth potential due to pathological factors, most commonly impaired placental trophoblast cell function. Currently, effective prevention and treatment methods of FGR are limited. We aimed to explore the pathogenesis of FGR and provide potential strategies for mitigating its occurrence.

miR-199a-3p mitigates simulated microgravity-induced cardiac remodeling by targeting MEF2C.

Microgravity-induced cardiac remodeling and dysfunction present significant challenges to long-term spaceflight, highlighting the urgent need to elucidate the underlying molecular mechanisms and develop precise countermeasures. Previous studies have outlined the important role of miRNAs in cardiovascular disease progression, with miR-199a-3p playing a crucial role in myocardial injury repair and the maintenance of cardiac function. However, the specific role and expression pattern of miR-199a-3p in microgravity-induced cardiac remodeling remain unclear.

Prevalence and associated factor of verbal abuse against nurses: A systematic review.

Background: Nurses who experience verbal abuse often report negative emotions, which can affect their work status and nurse-patient relationship. However, to the best of our knowledge, no study has summarized the prevalence of verbal abuse among nurses by different perpetrators and related risk factors.

Aim: This review aimed to synthesize the prevalence of verbal abuse among nurses and identify the most common sources and related risk factors.

Comparison of two-year myopia control efficacy between spectacle lenses with highly aspherical lenslets and orthokeratology lenses.

Purpose: To compare the two-year efficacy of spectacle lenses with highly aspherical lenslets (HAL) and orthokeratology (OK) lenses in managing myopia in children.

Methods: This retrospective study examined medical records from the Affiliated Eye Hospital of Wenzhou Medical University, involving 1683 HAL users and 1192 OK users. Participants were children aged 8-13 with a refractive error of -0.

Genomic insights into a multidrug-resistant Pandoraea apista clinical isolate carrying bla from China.

Objectives: Pandoraea apista is notable for its multidrug resistance and is frequently identified in patients with cystic fibrosis or other chronic lung diseases, where it contributes to persistent lung infections. In this study, we describe a strain of P. apista harboring the bla, isolated from the bronchoalveolar lavage (BAL) fluid of an inpatient in China.

Engineering Defect-Mediated S-scheme heterojunctions in MOF-Derived N-doped NiO porous microrods and ZnInS nanosheets for superior photocatalytic hydrogen evolution.

The development of efficient photocatalysts inspired by natural photosynthesis has drawn considerable interest for sustainable hydrogen (H) production. Among the various strategies for enhancing H evolution, constructing step-scheme (S-scheme) heterojunctions has attracted extensive interest, thanks to their limited charge recombination and enhanced charge transport in comparison to the traditional photocatalytic systems. Herein, we report the engineering of a novel S-scheme heterojunction by integrating ultrathin ZnInS (ZIS) nanosheets with MOF-derived N-doped NiO porous microrods (ZIS/N-NiO) toward superior photocatalytic behaviors.

Development and psychometric evaluation of a self-management ability assessment scale for individuals with spinal cord injury.

Objective: The objective of this study was to develop a scale to assess the self-management capability of patients with spinal cord injuries and provide an evaluation tool for implementing targeted interventions.

Methods: Drawing upon the Integrated Theory of Health Behavior Change (ITHBC) and the International Classification of Functioning, Disability, and Health (ICF) framework, an initial version of the Spinal Cord Injury Patient Self-Management Capability Assessment Scale was developed through literature analysis, semi-structured interviews, and expert inquiry using the Delphi method. Subsequently, two rounds of inquiries were conducted with a panel of 20 experts to refine items in the scale based on the feedback obtained.

Application of a near-infrared viscosity-responsive fluorescent probe for lysosomal targeting in fatty liver mice.

Viscosity is a fundamental property in biological systems, influencing organelle function and molecular diffusion. Abnormal viscosity is associated with diseases such as metabolic disorders, neurodegeneration, and cancer. Lysosomes, central to cellular degradation and recycling, are sensitive to viscosity changes, which can disrupt enzymatic activity and cellular homeostasis.

Chirality Interplay of Peptide and Saccharide on Glycopeptide Self-Assembly.

Saccharides and peptides with markedly disparate stereochemical features serve as pivotal chiral molecular partners in living systems. The importance of glycosylation in influencing glycopeptide self-assembly has been recognized. However, how different chiral combinations of saccharides and peptides influence the macroscopic hydrogel mechanics, fiber nanomechanics, asymmetric molecular packing, and thermodynamic changes during glycopeptide self-assembly remains unknown.

Nerve-Derived Extracellular Matrix Promotes Neural Differentiation of Bone Marrow Stromal Cells and Enhances Interleukin-4 Efficacy for Advanced Nerve Regeneration.

Facilitating neuronal differentiation of stem cells and microenvironment remodeling are the key challenges in cell-based transplantation strategies for central nervous system regeneration. Herein, the study harnesses the intrinsic pro-neural differentiation potential of nerve-derived extracellular matrix (NDEM) and its specific affinity for cytokines to develop an NDEM-gelatin methacryloyl(gelMA)-based bifunctional hydrogel delivery system for stem cells and cytokines. This system promotes the neural differentiation of bone marrow stromal cells (BMSCs) and optimizes the therapeutic index of Interleukin-4 (IL-4) for spinal cord injury (SCI) treatment.

Knockdown of CCNB1 alleviates high glucose-triggered trophoblast dysfunction during gestational diabetes via Wnt/β-catenin signaling pathway.

Gestational diabetes mellitus (GDM), defined as glucose intolerance occurring or first detected during pregnancy, affects approximately 8% of pregnancies worldwide. The dysfunction of trophoblasts in pregnancies complicated by GDM is associated with changes in trophoblast cell functions, resulting in compromised proliferation and regulation of the cell cycle. Cyclin B1 (CCNB1), a pivotal controller of the start of mitosis, is crucial in these mechanisms.

Preparation and characterization of a rat uterine decellularized scaffold.

Background: Infertility is a special reproductive health defect. For women, congenital uterine malformations, extensive adhesions in the uterine cavity, and hysterectomy are associated with infertility. Uterine transplantation is technically feasible, but its clinical application and development are limited by donor shortages and immune rejection.

Metabolic reprogramming and signaling adaptations in anoikis resistance: mechanisms and therapeutic targets.

Anoikis, a form of programmed cell death triggered by detachment from the extracellular matrix (ECM), maintains tissue homeostasis by removing mislocalized or detached cells. Cancer cells, however, have evolved multiple mechanisms to evade anoikis under conditions of ECM detachment, enabling survival and distant metastasis. Studies have identified differentially expressed proteins between suspended and adherent cancer cells, revealing that key metabolic and signaling pathways undergo significant alterations during the acquisition of anoikis resistance.

L-selectin Promotes Migration, Invasion and Inflammatory Response of Fibroblast-Like Synoviocytes in Rheumatoid Arthritis via NF-kB Signaling Pathway.

Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease characterized by chronic inflammation of the synovium and progressive joint damage. Fibroblast-like synoviocytes (FLSs) exhibit excessive proliferative and aggressive phenotypes and play a major role in the pathophysiology of RA. Previous studies have confirmed the pathologic role of L-selectin in cell adhesion and migration.

Effect of both the 9S management theory and the hierarchical management model on management of intensive care unit.

Objective: To explore the effect of the 9S management theory plus hierarchical management model on the management of intensive care unit (ICU).

Method: Ninety patients hospitalized in ICU from January 2021 to January 2023 were selected and divided into a control group (n = 45) and a study group (n = 45) according to different ward management methods. The management lasted for one month.

Non-linear dose-response relationship between uterine artery pulsatility index and risk of preeclampsia in early pregnancy: A secondary analysis based on a nested cohort study.

Background: Previous studies have shown that higher uterine artery pulsatility index (UtA-PI) values in early pregnancy have predictive value for the risk of preeclampsia (PE). However, the sensitivity and specificity of this marker remain controversial. This study aims to explore further the association between UtA-PI in early pregnancy and the incidence of preeclampsia.

Lipids, lipid-lowering drug target genes and pancreatic cancer: a Mendelian randomization study.

Background: Pancreatic cancer (PC) is a malignant tumor with a low survival rate. Lipid modifiers show potential for PC therapy, but evidence is lacking.

Aim: This Mendelian randomization (MR) study aimed to explore the relationship between lipid traits, and lipid-lowering drug target genes with PC risk.

Study on Immune-Related Genes and Clinical Validation of Acute Myocardial Infarction Based on Bioinformatics.

Acute myocardial infarction (AMI) is a cardiovascular disease featuring the narrowing and hardening of coronary arteries triggered by a combination of factors, which ultimately leads to the death of heart muscle. We retrieved the GSE109048 and GSE123342 datasets from the Gene Expression Omnibus (GEO) database. After integrating these datasets, we selected 154 module key genes with the help of weighted correlation network analysis (WGCNA).