Unnatural enzyme activation by a metal-responsive regulatory protein.

As a result of calcium ion binding, the calcium-dependent regulatory protein calmodulin (CaM) undergoes a conformational change, enabling it to bind to and activate a variety of enzymes. However, the detoxification enzyme glutathione -transferase (GST) is notably not among the enzymes activated by CaM. In this study, we demonstrate the feasibility of establishing, , an artificial regulatory link between CaM and GST using bifunctional chemical transducer (CT) molecules possessing binders for CaM and GST.