Changes in the prostate after the cryoapplication in dogs.

The research aimed at defining local changes in the healthy prostatic gland after the procedure of cryodestruction. In 15 dogs the gland was frozen unifocally in the double cycle from the outside using the flat applicator. At different times over the period of 6 months after the cryodestruction procedure particular dogs were subjected to surgery in order to determine macro- and microscopic changes.

Cryodestruction technique in the prostatic gland in dogs.

The investigation aimed at selecting the best parameters of cryodestruction of the healthy prostatic gland in dogs. Freezing was performed from the external side of the gland. The investigations were carried out on 2 healthy dogs in which the prostatic glands were frozen with two different methods and then, 4 weeks later, the degree of the gland size decrease was estimated.

Morphological and hormonal changes after superovulation in cows treated with Neutra-PMSG.

Morphological changes in ovaries and hormonal changes as well as changes in Na, K, Ca, Mg, and P blood plasma levels were observed after superovulation induced by the administration of PMSG and the neutralization of this hormone with monoclonal antibodies Neutra-PMSG administered either 72 or 108 h later. The introduction of Neutra-PMSG 108 h after PMSG injection clearly decreases the number of surviving non-ovulated follicles with a diameter > 10 mm (1.7 +/- 0.

Environmental genotoxicity and cancer risk in humans: a combined evaluation correlating the results of the Tradescantia micronucleus assay in the field and human biomarker assessments in serum. I. The TRAD-MCN assay.

It is well documented that environmental pollution from industrial activity, sewage farms, hazardous waste sites, incinerators, etc, contributes to the overall cancer risk and that this contribution can be considerable under certain circumstances. It is important, therefore, to identify the level of genotoxic activity in the environment and to relate it to biomarkers of cancer risk in humans. After reviewing a range of cytogenetic assays, we have selected the Tradescantia micronucleus assay (TRAD-MCN) developed by Ma et al to be used in indoor and field evaluations.

Purification and partial characterization of glutamine synthetase isoforms from Triticale seedlings.

Two isoforms of glutamine synthetase (EC 6.3.1.

Rumen degradability of dry matter and crude fibre of irradiated and sodium hydroxide treated straws.

Wheat straw (WS) and triticale straw (TS) were treated with 2- and 4%-NaOH and/or 300- and 600-kGy radiation doses (accelerated electrons) for improvement of the nutritional value of these feeds. Beside the chemical composition of straws, the nylon bag rumen disappearance of dry matter and crude fibre was estimated. NaOH did not influence the chemical composition of straws, while irradiation significantly reduced the level of crude fibre (CF), neutral detergent fibre (NDF) and acid detergent fibre (ADF) proportionally to the dose.

TGF-beta 1 inhibits polyamine biosynthesis in K 562 leukemic cells.

The present study proved that TGF-beta 1 significantly inhibited the growth of K 562 cells. The drop in cell numbers after 24 h incubation with increasing concentrations of TGF-beta 1 (0.01, 0.

Blood plasma pseudouridine in patients with malignant proliferative diseases.

The blood plasma concentration of pseudouridine was estimated in 104 healthy adult subjects, and 108 patients suffering from malignant proliferative diseases. The HPLC method for simultaneous determination of pseudouridine and creatinine was applied. The average physiological concentration of pseudouridine in blood plasma was 2.

Comparison of metabolic effects of EGF, TGF-alpha, and TGF-beta 1 in primary culture of fetal bovine myoblasts and rat L6 myoblasts.

1. Comparative studies of EGF, TGF-alpha, and TGF-beta 1 action on the synthesis of DNA and cellular proteins in rat L6 myogenic cells and fetal bovine myoblasts demonstrated considerable differences between particular growth factors, dependent on dose and target cells. 2.

Metabolic effect of orotic acid in calves.

Eight calves (males, Black and White crossbred with Holstein-Fresian) were fed milk and milk replacer without (control group) or with potassium orotate (3 mmol./l.) supplementation for 6 weeks after birth.

Comparative study of antibacterial activity of pancreatic juice in six mammalian species.

A comparative study of antibacterial activity of pancreatic juice was conducted on six mammalian species. Pancreatic juice collections were conducted as acute (rabbit, guinea pig, rat) and chronic (pig, sheep, cattle) experiments, in the former before and after stimulation [cholecystokinin (CCK) and secretin] and in the latter under basal conditions alone. Antibacterial activity was tested on Micrococcus pyogenes and compared with that of neomycin.

Periodic fluctuations in pancreatic secretion and duodenal motility investigated in neonatal calves.

To clarify the relative timing of clinical changes in duodenal motility and pancreatic secretion in newborn calves, we recorded duodenal electrical and mechanical activity and analysed pancreatic secretion and migrating myoelectric complex (MMC). In eight calves integrated recordings were derived from sites near the duodenal bulb and pancreatic accessory duct orifice, and pancreatic juice was sampled after an overnight fast, after a feed, and during reversible cold vagal blockade. Peak secretion coincided with duodenal irregular spiking activity and the nadir with absence of spiking.

Metabolic effect of orotic acid in rat L6 myoblasts.

Orotic acid (OA) is an intermediate in the pyrimidine pathway. The main source of OA in the human and animal diet is bovine milk and its products. OA significantly inhibited the stimulation of protein synthesis by FCS derived growth factors in L6 myoblasts.

Mitogenic effect of lymph, amniotic and allantoic fluid in bovine fetal myoblasts.

Amniotic and allantoic fluids, as well as afferent lymph (tissue fluid), stimulated DNA synthesis in bovine fetal myoblasts (BFM) at low concentrations (5%). Higher doses abolished the mitostimulatory effect of these fluids and led toward inhibition of [3H]mTdR incorporation into DNA. These effects were much more evident (particularly in the case of amniotic fluid) when fluids were lyophylized prior to the experiment.

Genetic factors influencing regeneration ability in rye (Secale cereale L.). I. Immature inflorescences.

Immature inflorescences of ten rye inbred lines (inbred degree S10 and S11) were cultured on solidified MS medium supplemented with 3.0 mg/dm(3) of 2,4-D. According to their capability for callus production explants were classified into two groups : responsive (giving weak or intensive callus production) and non responsive (lack of callus formation).

Effect of nitrite on ureagenesis and carbohydrate metabolism in isolated rat hepatocytes.

The effect of three NaNO2 concentrations (0.5, 2.0, and 5.

Comparison of urinary neopterin and pseudouridine in patients with malignant proliferative diseases.

The HPLC method for the simultaneous determination of urinary neopterin, pseudouridine, and creatinine allows a rapid evaluation of the activation state of cell-mediated immunity, and the stimulation of whole-body rRNA + tRNA turnover, associated with malignant growth. Urinary neopterin and pseudouridine concentrations in healthy subjects amounted to: 106.6 +/- 34.

Epizootic of equine influenza in 1969 in Poland.

Epidemiological observations on the course and spread of equine influenza in Poland during the 1969 epizootic were carried out. The dynamics of the spread of the disease in the country are shown graphically by voivodship. The disease incidence and mortality rates for the entire country are also shown.

Cholera toxin (choleragen)-polymorphonuclear leukocyte interactions: effect on migration in vitro and Fc gamma R-dependent phagocytic and bactericidal activity.

PMNL leukocytosis is a feature common to many types of infectious and inflammatory diseases. How PMNL are recruited to tissues is not yet clear although it is a question that has considerable clinical importance. We investigated the function of PMNL which migrated through an artificial barrier (Chinese hamster ovary (CHO) cells, collagen and nylon cloth membrane) subjected to CT or choleragenoid treatment toward plain medium (the same RPMI in the upper and lower chamber) or medium containing chemotactic factor (fMLP or LPS or ZAS).

Effect of intraduodenal HCl and soybean extract on pancreatic juice secretion during atropinization and cold vagal blockade in calves.

In order to elucidate the mechanisms by which intraduodenal hydrochloric acid (HCl) and soybean extract influence exocrine pancreatic secretion in the young ruminant, we conducted experiments repeatedly on six conscious calves with and without blockade of the extrinsic and intrinsic neural pathways. In the absence of blockade, each of the two stimuli increased the juice volume, the HCl effect being far stronger than that of soybean extract. Intrinsic cholinergic blockade by atropinization blocked the stimulatory work of soybean extract on pancreatic secretion and on HCl-stimulated protein but had a weak effect on the amount of juice evoked by HCl.

Determination of diaminopimelic acid in biological materials using high-performance liquid chromatography.

A method for the determination of diaminopimelic acid (DAPA) concentrations in feeds and rumen digesta by reversed-phase high-performance liquid chromatography using precolumn derivatization with o-phthaldialdehyde and fluorimetric detection was developed. Samples were oxidized and hydrolysed prior to analyses by HPLC. Hydrogen peroxide and formic acid were used for oxidation; hydrolyses were performed using 3 M hydrochloric acid under vacuum at 120 degrees C for 17 h.

Bikaverin production by Fusarium species.

Production of bikaverin has been examined in 130Fusarium isolates belonging to 21 species. The highest yield of bikaverin was produced on autoclaved rice - up to 2.5g/kg of dry culture.

The influence of age and sex on selenium distribution and glutathione peroxidase activity in plasma and erythrocytes of selenium-adequate and supplemented rats.

The experiment was performed on Sprague-Dawley male rats weighting 203, 103 and 53 g, and female 99 g. Animals were fed for 2 weeks a diet containing 0.1 and 2.

Localization of cauliflower mosaic virus in the cell nucleus of Brassica pekinensis L.

Cauliflower mosaic virus (CaMV) particles were observed in the nuclei of xylem parenchyma cells in Brassica pekinensis L. doubly infected by CaMV and turnip mosaic virus (TuMV). CaMV particles were aggregated in the nucleoplasm but not embedded in viroplasms.

Selenium content and distribution in bovine and human milk from different regions of Poland.

Human milk and bulk cow's milk samples from 3 different areas of central Poland were analyzed for their Se content. Cow's milk specimens were also analyzed for Se distribution and GSH-Px activity. Mature human milk contained 34-68% more Se than bulk cow's milk obtained from analogous areas of central Poland.