Sulfate-Reducing Bacteria Isolated from an Oil Field in Kazakhstan and a Description of sp. nov.

Sulfidogenic bacteria cause numerous issues in the oil industry since they produce sulfide, corroding steel equipment, reducing oil quality, and worsening the environmental conditions in oil fields. The purpose of this work was to isolate and taxonomically identify the sulfidogenic bacteria responsible for the corrosion of steel equipment at the Karazhanbas oil field (Kazakhstan). In this study, we characterized five sulfidogenic strains of the genera , , and isolated from the formation water of the Karazhanbas oil field (Kazakhstan).

The Phytochemical Properties of Low-Grade Longan Syrup and Its Potential Use as a Dietary Supplement for Honey Bees.

Climate change significantly affects honey bee populations and their access to natural food sources, demanding alternative economic feed sources. Longan stands out as the most important fruit crop in Southeast Asia, but with a surplus of low-grade fruit that is not suitable for the market. This study investigates the potential of longan syrup as an alternative carbohydrate source for honey bees by measuring sugar composition, phytochemical profiles, feed, and survival, as well as the resulting gut microbial changes.

Measuring the Risk of Violence Through Health Surveillance.

Workplace violence (WV) is a ubiquitous, yet under-reported and under-studied phenomenon. Prevention measures may be ineffective because risk assessment is often based on unvalidated algorithms. After monitoring the risk of WV in a healthcare company for over 20 years, this paper presents the results collected in 2023 and details of the methodology used.

Mental Health Literacy and Attitudes Towards Mental Health Problems Among College Students, Nepal.

(1) Background: Research on mental health literacy (MHL) and attitudes toward mental health problems (ATMHP) among non-medical college students in Nepal is limited. This study examined the relationship between MHL and ATMHP, considering demographic variables and familiarity with mental health issues; (2) Methods: We conducted a cross-sectional survey with 385 college students from Chitwan and Kathmandu, Nepal, using opportunity sampling. Descriptive and inferential statistics examined demographic differences, while Pearson's correlation assessed relationships among latent variables; (3) Results: No relationship was found between MHL and ATMHP (r = -0.

Bioactive Compounds, Antioxidant Properties, and Antimicrobial Profiling of a Range of West Algerian Honeys: In Vitro Comparative Screening Prior to Therapeutic Purpose.

Honey is a complex natural nutrient with well-established therapeutic properties recognized in traditional medicine. The purpose of the current work was to compare, in vitro, the bioactive compounds, antioxidants, and antimicrobial properties of 37 honey samples collected from the western region of Algeria and to identify the best sample for potential therapeutic purposes. Estimation of bioactive compounds was carried out by determining the total phenolic and flavonoid contents.

Enhanced Antioxidant and Digestive Enzyme Inhibitory Activities of Pacific White Shrimp Shell Protein Hydrolysates via Conjugation with Polyphenol: Characterization and Application in Surimi Gel.

Pacific white shrimp shell protein hydrolysates (SSPHs) produced using alcalase (UAH) and papain (UPH), and polyphenols (PPNs) conjugates were prepared using variable concentrations (0.5-3% /) of different polyphenols (EGCG, catechin, and gallic acid). When 2% (/) of a redox pair was used for conjugation, 0.

Developing a Simple and Feasible Process for the Crude Extraction of Livetins and Phosvitin from Egg Yolk.

Due to imbalanced demand favoring egg whites, the egg industry faces a surplus of egg yolk, limiting overall growth. This study designed a feasible process for the crude extraction of livetins and phosvitin (PV) and revealed the related separation mechanisms. Our method utilized a 1:9 egg yolk dilution at pH 6.

Evaluation of Anti-Angiogenic Therapy Combined with Immunotherapy and Chemotherapy as a Strategy to Treat Locally Advanced and Metastatic Non-Small-Cell Lung Cancer.

Immunotherapy has made recent improvements in disease-free survival (DFS) and/or overall survival (OS) in all stages of non-small-cell lung cancer (NSCLC). Here, we review the tumor microenvironment and its immunosuppressive effects and discuss how anti-angiogenic therapies may potentiate the anti-carcinogenic effects of immunotherapy. We also review all the past literature and discuss strategies of combining anti-angiogenic therapy and immunotherapy +/- chemotherapy and hypothesize how we can use this strategy for non-small-cell lung cancer in metastatic previously untreated/previously treated settings in previously treated EGFR-mutated NSCLC for the upfront treatment of brain metastases prior to radiation therapy and for the incorporation of this strategy into stage III unresectable disease.

Gillies ex Hooker et Arnott (Oxalidaceae): Chemical Analysis, Biological In Vitro and In Vivo Properties and Behavioral Effects.

In this work, a decoction (DOe) and a methanolic global extract (MGEOe), obtained with the aerial parts of Gillies ex Hooker et Arnott (Oxalidaceae), were evaluated. The high-resolution liquid chromatography in conjunction with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS) analysis showed forty compounds in MGEOe and twenty-nine in DOe, including flavones, C-glycosyl flavones, isoflavones, fatty acids, terpenes, phenolic acids, and sterols. The antioxidant properties were evaluated by DPPH, TEAC, FRAP, and ILP assays.

Impact of pulmonary hypertension on short and long-term outcome after mitral transcatheter edge-to-edge repair: A meta-analysis.

Background: Pulmonary hypertension (pHTN) has been associated with increased morbidity and mortality after mitral Transcatheter Edge-to-Edge Repair (TEER), but the association remains uncertain. This study aims to evaluate the impact of pHTN on cardiovascular outcomes following TEER.

Methods: We searched PubMed, Scopus, and Medline to identify studies reporting outcomes after TEER in individuals with pHTN.

3,6-Anhydro-L-galactose suppresses mouse lymphocyte proliferation by attenuating JAK-STAT growth factor signal transduction and G-S cell cycle progression.

Recombinant GH16B β-agarase-catalyzed liquefaction of 5-7 %(w/v) melted agarose at 50 °C completely hydrolyzed agarose into neoagarohexaose (NA6) and neoagarotetraose (NA4). Subsequent saccharification by recombinant GH50A β-agarase or recombinant GH50A β-agarase/recombinant GH117A α-neoagarobiose hydrolase at 35 °C converted NA6/NA4 into neoagarobiose (NA2) or 3,6-anhydro-L-galactose (L-AHG)/D-galactose, respectively. Purification of NA6/NA4 and NA2 was achieved by Sephadex G-15 column chromatography, while L-AHG was purified by Sephadex G-10, achieving ≥ 98 % purity.

3D Printing of a Self-Healing, Bioactive, and Dual-Cross-Linked Polysaccharide-Based Composite Hydrogel as a Scaffold for Bone Tissue Engineering.

Although 3D printing is becoming a dominant technique for scaffold preparation in bone tissue engineering (TE), developing hydrogel-based ink compositions with bioactive and self-healing properties remains a challenge. This research focuses on developing a bone scaffold based on a composite hydrogel, which maintains its self-healing properties after incorporating bioactive glass and is 3D-printable. The plain hydrogel ink was synthesized using natural polymers of 1 wt % N-carboxyethyl chitosan, 2 wt % hyaluronic acid aldehyde, 0.

Regulation of human Th9 cell differentiation by lipid modulators targeting PPAR-γ and acetyl-CoA-carboxylase 1.

CD4 T cell activation induces dramatic changes to cellular metabolism for supporting their growth and differentiation into effector subsets. While the cytokines IL-4, TGF-β and IL-21 promote differentiation into Th9 cells, metabolic factors regulating this process remain poorly understood. To assess the role of lipid metabolism in human Th9 cell differentiation, naïve CD4 T cells were purified from blood of healthy volunteers and cultured in the presence or absence of compounds targeting PPAR-γ, acetyl-CoA-carboxylase 1 (ACC1), and AMP-activated protein kinase (AMPK) for four days.

The relative cerebral blood volume (rCBV) < 42% is independently associated with hemorrhagic transformation in anterior circulation large vessel occlusion.

Background: Pretreatment CT perfusion (CTP) marker relative cerebral blood volume (rCBV) < 42% lesion volume has recently shown to predict poor collateral status and poor 90-day functional outcome. However, there is a paucity of studies assessing its association with hemorrhagic transformation (HT). Here, we aim to assess the relationship between rCBV < 42% lesion volume with HT.

A new disease symptomology observed in wheat is associated with high levels of Triticum mosaic virus transcripts.

Wheat is an important cereal crop globally and in the United States, and is the largest crop grown by acreage in Colorado. In June 2023, we observed wheat fields displaying severe yellowing and virus-like disease symptoms in plants across seven eastern Colorado counties (Yuma, Prowers, Kit Carson, Washington, Sedgewick, Morgan, and Weld). Symptomatic plants were prominent in fields and appeared bright yellow, with ringspots, mosaic patterning, and streaking on leaves.

Sustainable oxalic acid treatment of lignocellulosic fibers for ensuring improved performance of treated fibers based bio PBS composites.

Plant-based macromolecules such as lignocellulosic fibers are one of the promising bio-resources to be utilized as reinforcement for developing sustainable composites. However, due to their hydrophilic nature and weak interfacial bonding with polymer matrices, these fibers are mostly incompatible with biopolymers. The current research endeavor explores the novel eco-friendly oxalic acid (CHO.

Polydimethylsiloxane loaded capsaicin afflicts membrane integrity, metabolic activity and biofilm formation of nosocomial pathogens.

Biofilms constitute 80% of all nosocomial infections associated with invasive medical devices. Polydimethylsiloxane, a highly elastic, inert, non-reactive, biocompatible silicone polymer is widely used as implant biomaterial due to its non-toxic and low-immunogenic nature. Owing to its hydrophobicity, PDMS suffers from microbial adhesion.

Diagnosis and Management of Legg-Calvé-Perthes Disease in the Obese Pediatric Population.

Legg-Calvé-Perthes Disease (LCPD) is a condition marked by temporary blood flow disruption to the proximal femur, commonly afflicting children aged 15 and younger. The etiology of the disease is often idiopathic and involves the development of avascular necrosis of the femoral head, subsequently leading to bone weakening and deformity. Obesity exacerbates LCPD, correlating with delayed diagnosis, increased disease severity, and bilateral involvement.

Accelerating biosimilar market access: the case for allowing earlier standing.

Biosimilars, which are affordable alternatives to biologic medicines, face delays in market entry due to the current patent litigation framework under the Biologic Price Competition and Innovation Act. Currently, biosimilar manufacturers can only initiate patent litigation to attempt to clear weak and invalid patents after submitting their Biologic License Application to the Food and Drug Administration (FDA), which happens after completing extensive, and costly clinical trials. By contrast, generic drug manufacturers can start litigation earlier due to shorter development times and less stringent clinical requirements, allowing them to launch immediately after the primary patent expires.

Employer Perspective on Pain Fellowship Education: A Survey to Understand the Current State of Pain Medicine Training.

Background: Pain medicine care has expanded to encompass a wider range of conditions, necessitating updated education and training for pain specialists to utilize emerging technologies effectively. A national survey was conducted through several verified Pain organizations regarding pain physician employers' perspectives on pain medicine fellowship training and education. The survey aimed to gather insights from a diverse range of geographic locations, practice types (academic and private practice), and practice settings.

Data on hydrodynamic flow and aspiration mechanisms in a patient-specific pharyngolaryngeal model with variable epiglottis angles.

This dataset comprises a comprehensive collection of videos and images illustrating the fluid dynamics of swallowing and aspiration in a patient-specific pharyngolaryngeal model with varying epiglottis angles. The data also includes the physical properties of the fluids used, comprising dynamic viscosity, surface tension, and contact angle. Videos under varying swallowing conditions were collected to investigate the mechanisms underlying aspiration.

Enhanced stability and oxidation resistance of Bunge seed oil Pickering emulsion using rice bran protein modified by phytic acid.

The potential applications of Bunge seed oil in the food and medical industries are constrained by its susceptible fatty acid composition, which is prone to oxidation. In this study, rice bran protein (RBP) was employed as an emulsifier for the fabrication of Bunge seed oil Pickering emulsion. The impact of antioxidant-phytic acid (PA) on the stability of Pickering emulsion and the underlying mechanisms were further investigated.

sp. nov., a Al-tolerant bacterium with the ability to inhibit f. sp. isolated from rhizosphere soil of muskmelon.

A bacterial strain, designated as A6, was isolated from the rhizosphere soil of a healthy muskmelon in Wenchang, Hainan Province, China. The cells of strain A6 were Gram-negative, aerobic, short rod and motile with a single polar flagellum. Strain A6 could tolerate up to 55.

Collagen/polyvinyl alcohol scaffolds combined with platelet-rich plasma to enhance anterior cruciate ligament repair.

In anterior cruciate ligament (ACL) repair methods, the continuous enzymatic erosion of synovial fluid can impede healing and potentially lead to repair failure, as well as exacerbate articular cartilage wear, resulting in joint degeneration. Inspired by the blood clot during medial collateral ligament healing, we developed a composite scaffold comprising collagen (1 %, w/v) and polyvinyl alcohol (5 %, w/v) combined with platelet-rich plasma (PRP). The composite scaffold provides a protective barrier against synovial erosion for the ruptured ACL, while simultaneously facilitating tissue repair, thereby enhancing the efficacy of ACL repair techniques.

Substrate-specific responses to mixing conditions in high-solids enzymatic hydrolysis: Insights from microcrystalline cellulose and dilute-acid pretreated corncob.

This study investigates the mixing effects on the enzymatic hydrolysis of microcrystalline cellulose (MCC) and dilute-acid pretreated corncob substrates under high-solid conditions. Enzymatic hydrolysis experiments were conducted to assess cellulose conversion rates under varying mixing conditions (0, 50, 150, and 250 rpm) and solids loadings (5 %, 15 %, 25 %, and 35 %, w/v), and distinct physicochemical properties of the substrates were characterized. Additionally, the role of mixing conditions and solid loadings on cellulose hydrolysis kinetics and enzyme adsorption on both substrates and lignin were elucidated.