74 results match your criteria: "WA D.Y.; and Novartis Institutes for BioMedical Research[Affiliation]"
Autoantibody Landscape in Patients with Advanced Prostate Cancer.
Clin Cancer Res
December 2020
Department of Radiation Oncology, University of California San Francisco, San Francisco, California.
Purpose: Autoantibody responses in cancer are of great interest, as they may be concordant with T-cell responses to cancer antigens or predictive of response to cancer immunotherapies. Thus, we sought to characterize the antibody landscape of metastatic castration-resistant prostate cancer (mCRPC).
Experimental Design: Serum antibody epitope repertoire analysis (SERA) was performed on patient serum to identify tumor-specific neoepitopes.
Conserved Interferon-γ Signaling Drives Clinical Response to Immune Checkpoint Blockade Therapy in Melanoma.
Cancer Cell
October 2020
Jonsson Comprehensive Cancer Center at the University of California, Los Angeles (UCLA), Los Angeles, CA, USA; Parker Institute for Cancer Immunotherapy, San Francisco, CA, USA. Electronic address:
We analyze the transcriptome of baseline and on-therapy tumor biopsies from 101 patients with advanced melanoma treated with nivolumab (anti-PD-1) alone or combined with ipilimumab (anti-CTLA-4). We find that T cell infiltration and interferon-γ (IFN-γ) signaling signatures correspond most highly with clinical response to therapy, with a reciprocal decrease in cell-cycle and WNT signaling pathways in responding biopsies. We model the interaction in 58 human cell lines, where IFN-γ in vitro exposure leads to a conserved transcriptome response unless cells have IFN-γ receptor alterations.
View Article and Find Full Text PDFDefinition, aims, and implementation of GA LEN/HAEi Angioedema Centers of Reference and Excellence.
Allergy
August 2020
HAE International (HAEi), Fairfax City, VA, USA.
Comparison of Multivendor Single-Voxel MR Spectroscopy Data Acquired in Healthy Brain at 26 Sites.
Radiology
April 2020
From the Division of Neuroradiology, Park 367B, Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, 600 N Wolfe St, Baltimore, MD 21287 (M.P., M.M., A.B., K.L.C., G.O., N.A.J.P., M.G.S., R.A.E.E., P.B.B.); F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD (M.P., M.M., A.B., K.L.C., G.O., N.A.J.P., M.G.S., R.A.E.E., P.B.B.); Imaging Institute, Cleveland Clinic Foundation, Cleveland, OH (P.K.B.); Department of Radiology, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH (P.K.B.); Department of Radiology, Haukeland University Hospital, Bergen, Norway (M.K.B.); Spinoza Centre for Neuroimaging, Amsterdam, the Netherlands (P.F.B., D.S.); Department of Radiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH (K.M.C.); Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD (K.L.C.); Department of Radiology, Taipei Medical University Shuang Ho Hospital, New Taipei City, Taiwan (D.Y.T.C.); Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan (D.Y.T.C.); Department of Biological and Medical Psychology, University of Bergen, Bergen, Norway (A.R.C., L.E.); NOreMENT-Norwegian Center for Mental Disorders Research, University of Bergen, Bergen, Norway (A.R.C., L.E.); Movement Control & Neuroplasticity Research Group, Department of Movement Sciences, Group of Biomedical Sciences, KU Leuven, Leuven, Belgium (K.C., C.M., S.P.S.); REVAL Rehabilitation Research Center, Hasselt University, Diepenbeek, Belgium (K.C.); Department of Radiology, Medical Physics, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany (M.D., T.L.); Brain and Consciousness Research Centre, Taipei Medical University, Taipei, Taiwan (N.W.D.); School of Health Sciences, Purdue University, West Lafayette, IN (U.D., D.A.E., R.M.); Department of Radiology and Imaging Sciences, Indiana University School of Medicine, Indianapolis, IN (U.D., D.A.E.); Department of Neuroimaging, Central Institute of Mental Health, Mannheim, Germany (G.E., M.S.); Department of Clinical Engineering, Haukeland University Hospital, Bergen, Norway (A.R.C., L.E.); Department of Clinical and Health Psychology, University of Florida, Gainesville, FL (M.A.F., E.C.P., A.J.W.); Center for Cognitive Aging and Memory, McKnight Brain Institute, University of Florida, Gainesville, FL (M.A.F., E.C.P., A.J.W.); Shandong Medical Imaging Research Institute, Shandong University, Jinan, China (F.G., G.W.); Department of Human Physiology, University of Oregon, Eugene, OR (I.G.); Department of Radiology, University of Calgary, Calgary, Canada (A.D.H.); Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (N. He, Y.L., H.X., F.Y.); Department of Neurology, BG University Hospital Bergmannsheil, Bochum, Germany (S.H., M.T.); Academic Unit of Radiology, University of Sheffield, Sheffield, England (N. Hoggard, I.D.W.); Department of Radiology, Taipei Veterans General Hospital, National Yang-Ming University School of Medicine, Taipei, Taiwan (T.W.H., J.K.L., J.F.L.); Department of Radiology, Maastricht University Medical Center, Maastricht, the Netherlands (J.F.A.J.); Department of Psychiatry, Columbia University, New York, NY (A.K.. M.M.O.); New York State Psychiatric Institute, New York, NY (A.K., F.L.); GE Healthcare, Calgary, Canada (R.M.L.); GE Healthcare, Taipei, Taiwan (C.Y.E.L.); Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL (J.R.L.); National High Magnetic Field Laboratory, Gainesville, FL (J.R.L.); Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, MN (R.M.); Department of Psychology, University of Washington, Seattle, WA (S.O.M., M.P.S.); Center for Mind and Brain, University of California, Davis, Davis, CA (S.N.); GE Healthcare, Berlin, Germany (R.N.); Department of Electrical and Computer Engineering, McMaster University, Hamilton, Canada (M.D.N.); Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, SC (J.J.P.); Department of Radiology, Children's Hospital of Philadelphia, Philadelphia, PA (T.P.L.R.); Research Imaging Centre, Centre for Addiction and Mental Health, Toronto, Canada (N. Sailasuta, P.T.); Department of Psychiatry, University of Toronto, Toronto, Canada (N. Sailasuta); Department of Psychiatry and Behavioral Sciences, University of Minnesota, Minneapolis, MN (M.P.S.); School of Biomedical Engineering, McMaster University, Hamilton, Canada (N. Simard); Leuven Brain Institute (LBI), KU Leuven, Leuven, Belgium (S.P.S.); Department of Diagnostic and Interventional Radiology, Medical Faculty, Heinrich-Heine-University, Duesseldorf, Germany (H.J.W., H.J.Z.); Department of Functional Neurosurgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (C.Z.); Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD (A.Y.); and Institute of Clinical Neuroscience and Medical Psychology, Medical Faculty, Heinrich-Heine-University, Duesseldorf, Germany (H.J.Z.).
Background The hardware and software differences between MR vendors and individual sites influence the quantification of MR spectroscopy data. An analysis of a large data set may help to better understand sources of the total variance in quantified metabolite levels. Purpose To compare multisite quantitative brain MR spectroscopy data acquired in healthy participants at 26 sites by using the vendor-supplied single-voxel point-resolved spectroscopy (PRESS) sequence.
View Article and Find Full Text PDFMapping 123 million neonatal, infant and child deaths between 2000 and 2017.
Nature
October 2019
Institute for Health Metrics and Evaluation, University of Washington, Seattle, WA, USA.
Since 2000, many countries have achieved considerable success in improving child survival, but localized progress remains unclear. To inform efforts towards United Nations Sustainable Development Goal 3.2-to end preventable child deaths by 2030-we need consistently estimated data at the subnational level regarding child mortality rates and trends.
View Article and Find Full Text PDFA Phase 2 Randomized, Sham-Controlled Trial of Internal Carotid Artery Infusion of Autologous Bone Marrow-Derived ALD-401 Cells in Patients With Recent Stable Ischemic Stroke (RECOVER-Stroke).
Circulation
January 2019
Unicorn Pharma Consulting of Brentwood, TN (J.M.H.).
Background: Ischemic stroke has no approved treatments to enhance recovery. ALD-401 is an enriched population of aldehyde dehydrogenase-bright stem cells, capable of reducing neurological deficits in animal models. The primary objective of this trial was to determine the safety of internal carotid artery, intra-arterially delivered autologous bone marrow-derived ALD-401 in patients with disabling middle cerebral artery stroke in comparison with sham harvest with sham infusion.
View Article and Find Full Text PDFLarge-scale neuroanatomy using LASSO: Loop-based Automated Serial Sectioning Operation.
PLoS One
April 2019
Allen Institute for Brain Science, Seattle, WA, United States of America.
Serial section transmission electron microscopy (ssTEM) is the most promising tool for investigating the three-dimensional anatomy of the brain with nanometer resolution. Yet as the field progresses to larger volumes of brain tissue, new methods for high-yield, low-cost, and high-throughput serial sectioning are required. Here, we introduce LASSO (Loop-based Automated Serial Sectioning Operation), in which serial sections are processed in "batches.
View Article and Find Full Text PDFEndovascular Treatment in the DEFUSE 3 Study.
Stroke
August 2018
From the Departments of Radiology and Neurology, Stanford Stroke Center, CA (M.P.M., J.J.H., M.G.L., S.K., S.C., S.H., M.M., G.W.A.).
Background and Purpose- Endovascular therapy in an extended time window has been shown to be beneficial in selected patients. This study correlated angiographic outcomes of patients randomized to endovascular therapy with clinical and imaging outcomes in the DEFUSE 3 study (Endovascular Therapy Following Imaging Evaluation for Ischemic Stroke 3). Methods- Angiograms were assessed for the primary arterial occlusive lesion and the modified Thrombolysis in Cerebral Infarction (TICI) score at baseline and the final modified TICI score.
View Article and Find Full Text PDFPD-1 Blockade with Cemiplimab in Advanced Cutaneous Squamous-Cell Carcinoma.
N Engl J Med
July 2018
From the Departments of Dermatology and Head and Neck Surgery, University of Texas MD Anderson Cancer Center, Houston (M.R.M.), and South Texas Accelerated Research Therapeutics (START), San Antonio (K.P.P.) - both in Texas; the Department of Medical Oncology, Peter MacCallum Cancer Centre and University of Melbourne, Melbourne, VIC (D.R., A.A.T.), the Department of Medical Oncology, Royal North Shore Hospital, St. Leonards, VIC (A.G.), the Department of Medical Oncology, Sir Charles Gairdner Hospital, Perth, WA (A.M.L.), and Royal Brisbane and Women's Hospital and University of Queensland, Brisbane (B.G.M.H.) - all in Australia; the Department of Dermatology, Brigham and Women's Hospital (C.D.S.), the Department of Dermatology, Harvard Medical School (C.D.S.), and the Department of Medical Oncology, Dana-Farber Cancer Institute (G.R.) - all in Boston; Schleswig-Holstein University Hospital, Kiel (A.H.), and University Hospital Essen, Essen and German Cancer Consortium, Essen (D.S.) - both in Germany; University of Colorado Denver, School of Medicine, Aurora (K.D.L.); the Departments of Head and Neck-Endocrine Oncology (C.H.C.) and Cutaneous Oncology (N.I.K.), H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL; the Division of Medical Oncology, Department of Medicine, Washington University School of Medicine, St. Louis (L.H.-A.); the Department of Dermatology, Stanford University School of Medicine, Redwood City (A.L.S.C.), and the Division of Dermatology, City of Hope, Duarte (B.M.) - both in California; the Department of Medicine, Head and Neck Medical Oncology, Memorial Sloan Kettering Cancer Center, New York (L.A.D.), and Regeneron Pharmaceuticals, Tarrytown (F.S., M.M., K.M., G.D.Y., I.L., M.G.F.) - both in New York; Regeneron Pharmaceuticals, Basking Ridge, NJ (B.G., S.L., J.L., J.B., E.S.); University of Arizona Cancer Center, Tucson (H.M.B.), and Banner MD Anderson Cancer (J.H.) and the Department of Medical Oncology, Banner MD Anderson Cancer Center (J.N.), Gilbert - all in Arizona; Medical Oncology Department, Vall D'Hebron University Hospital (I.B.), and Institut Català D'Oncologia, Oncobell Program (IDIBELL), L'Hospitalet de Llobregat (M.G.-M.), Barcelona, and START Madrid-FJD, Hospital Fundación Jiménez Díaz, Madrid (V.M.) - all in Spain; Sarah Cannon Research Institute, Nashville (M.L.J.); and the Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University, Atlanta (T.K.O.).
Background: No systemic therapies have been approved for the treatment of advanced cutaneous squamous-cell carcinoma. This cancer may be responsive to immune therapy, because the mutation burden of the tumor is high and the disease risk is strongly associated with immunosuppression. In the dose-escalation portion of the phase 1 study of cemiplimab, a deep and durable response was observed in a patient with metastatic cutaneous squamous-cell carcinoma.
View Article and Find Full Text PDFThrombectomy for Stroke at 6 to 16 Hours with Selection by Perfusion Imaging.
N Engl J Med
February 2018
From the Departments of Neurology and Neurological Sciences (G.W.A., S. Kemp, S.C., J.P.T., S.H., M.M., M.G.L.), Diagnostic Radiology (M.P.M., J.J.H., G.Z.), Radiology (R.B.), and Biomedical Data Science (P.W.L.), Stanford University School of Medicine, Stanford, and the Department of Neurology, Keck School of Medicine, University of Southern California, Los Angeles (M.K.-T.) - both in California; the Departments of Neurology, Anesthesia, Neurosurgery, and Radiology, University of Iowa, Ames (S.O.-G.); the Departments of Diagnostic Imaging, Neurology, and Neurosurgery, Warren Alpert School of Medicine at Brown University and Rhode Island Hospital, Providence (R.A.M.); the Departments of Neurology and Neurosurgery, Ohio State University, Columbus (M.T.T.), and the University of Cincinnati Gardner Neuroscience Institute and the Department of Neurology and Rehabilitation Medicine, University of Cincinnati College of Medicine, Cincinnati (J.C., J.P.B.) - both in Ohio; the Departments of Neurosurgery and Neurology, Massachusetts General Hospital, Boston (T.L.-M.); the Department of Neurology, University of Texas Health Science Center, Houston (A.S.); the Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia (S.E.K.); the Departments of Radiology, Neurology, and Neurological Surgery, Northwestern University, Feinberg School of Medicine, Chicago (S.A.A.); the Department of Public Health Sciences, Medical University of South Carolina, Charleston (S.D.Y., Y.Y.P.); the Department of Neurology, New York University School of Medicine, New York (S. Kim); and the Departments of Clinical Neurosciences and Radiology, Hotchkiss Brain Institute, University of Calgary Cumming School of Medicine, Calgary, AB, Canada (A.M.D.).
Article Synopsis
- Thrombectomy is now being explored for patients with stroke who are treated between 6 to 16 hours after symptom onset, focusing on those with salvageable brain tissue and specific criteria for eligibility.
- A clinical trial evaluated its effectiveness, finding that patients receiving thrombectomy plus medical therapy had better functional outcomes and lower mortality rates compared to those receiving only medical therapy.
- The trial involved 182 patients across 38 U.S. centers and indicated significant improvements in independence and lower death rates for the thrombectomy group, although there were no notable differences in serious complications.
High-throughput characterization of protein-protein interactions by reprogramming yeast mating.
Proc Natl Acad Sci U S A
November 2017
Electrical Engineering Department, University of Washington, Seattle, WA 98195
High-throughput methods for screening protein-protein interactions enable the rapid characterization of engineered binding proteins and interaction networks. While existing approaches are powerful, none allow quantitative library-on-library characterization of protein interactions in a modifiable extracellular environment. Here, we show that sexual agglutination of can be reprogrammed to link interaction strength with mating efficiency using synthetic agglutination (SynAg).
View Article and Find Full Text PDFMetabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals.
Nat Commun
May 2017
Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, Gothenburg SE-41296, Sweden.
Production of chemicals and biofuels through microbial fermentation is an economical and sustainable alternative for traditional chemical synthesis. Here we present the construction of a Saccharomyces cerevisiae platform strain for high-level production of very-long-chain fatty acid (VLCFA)-derived chemicals. Through rewiring the native fatty acid elongation system and implementing a heterologous Mycobacteria FAS I system, we establish an increased biosynthesis of VLCFAs in S.
View Article and Find Full Text PDFRadical induced intermolecular linkage and energy level modifications of a porphyrin monolayer.
Chem Commun (Camb)
January 2017
Department of Chemistry and Materials Science & Engineering Program, Washington State University, Pullman, WA 99164, USA.
A new method to directly modify the surface structure and energy levels of a porphyrin monolayer was examined in the molecular scale using scanning tunneling microscopy and spectroscopy (STM and STS) and presented in this communication. The exposure to atomic oxygen has induced highly ordered surface cross-linking and changed the occupied and unoccupied orbital levels of a cobalt(ii) octaethyl porphyrin (CoOEP) monolayer, and as a result, the HOMO-LUMO gap was reduced by ∼10%. Counterintuitively, the STM/STS data indicated that the reactive central Co atoms did not participate in the gas-surface reactions.
View Article and Find Full Text PDFPower of an Adaptive Trial Design for Endovascular Stroke Studies: Simulations Using IMS (Interventional Management of Stroke) III Data.
Stroke
December 2016
From the Stanford Stroke Center, School of Medicine (M.G.L., N.S.B., G.W.A.), Department of Statistics (T.L.L., P.W.L.), Department of Biomedical Data Science, School of Medicine (T.L.L., P.W.L.), Stanford University, CA; Department of Public Health Sciences, Medical University of South Carolina, Charleston (S.D.Y., Y.Y.P.); and Department of Neurology, University of Cincinnati Medical Center, OH (J.P.B.).
Background And Purpose: Adaptive trial designs that allow enrichment of the study population through subgroup selection can increase the chance of a positive trial when there is a differential treatment effect among patient subgroups. The goal of this study is to illustrate the potential benefit of adaptive subgroup selection in endovascular stroke studies.
Methods: We simulated the performance of a trial design with adaptive subgroup selection and compared it with that of a traditional design.
Ribociclib as First-Line Therapy for HR-Positive, Advanced Breast Cancer.
N Engl J Med
November 2016
From the University of Texas M.D. Anderson Cancer Center, Houston (G.N.H.), and Texas Oncology-Baylor Charles A. Sammons Cancer Center and the U.S. Oncology Network, Dallas (J.O.) - all in Texas; Davidoff Center, Rabin Medical Center, Tel Aviv University, Tel Aviv (S.M.S.), and Sheba Medical Center, Ramat Gan (S.P.-S.) - both in Israel; the Sarah Cannon Research Institute (H.A.B., D.Y.), Vanderbilt-Ingram Cancer Center (C.L.A.), and Tennessee Oncology (D.Y.) - all in Nashville; National Cancer Center Singapore, Singapore (Y.-S.Y.); Netherlands Cancer Institute and BOOG Study Center, Amsterdam (G.S.S.); Institut de Cancérologie de l'Ouest/René Gauducheau, Saint-Herblain (M.C.), Institut Gustave Roussy, Université Paris Sud, Villejuif (F.A.), University Hospital of Besançon, Besançon (C.V.), and Centre Léon Bérard, Lyon (T.B.) - all in France; Duke University Medical Center, Durham, NC (K.L.B.); Dana-Farber Cancer Institute, Boston (E.P.W.); University of Ulm, Ulm (W.J.), Onkologische Praxis, Velbert (A.N.), University of Tübingen, Tübingen (E.-M.G.), and Joint Practice for Interdisciplinary Oncology and Hematology, Freiburg (N.M.) - all in Germany; Tom Baker Cancer Centre, Calgary, AB, Canada (S.V.); University of Padua and Istituto Oncologico Veneto, Istituto di Ricovero e Cura a Carattere Scientifico, Padua, Italy (P.C.); Edinburgh Cancer Research Centre, University of Edinburgh, Edinburgh (D.A.C.); Masaryk Memorial Cancer Institute, Brno, Czech Republic (K.P.); Florida Cancer Specialists-Sarah Cannon Research Institute, Fort Myers (L.L.H.); Breast Cancer Research Centre-Western Australia and Curtin University, Perth, Australia (A.C.); Department of Oncology, Vejle Hospital, Vejle, Denmark (E.J.); Arkhangelsk Clinical Oncology Dispensary, Arkhangelsk, Russia (O.B.); Hospital Universitario Virgen de la Victoria, Institute of Biomedical Research in Málaga, Málaga, Spain (E.A.); Oslo University Hospital, Oslo (E.W.); Virginia Cancer Specialists, Arlington (A.M.F.); Taipei Veterans General Hospital, National Yang-Ming University, Taipei, Taiwan (L.-M.T.); Rainier Hematology-Oncology, Northwest Medical Specialties, Puyallup, WA (S.B.); Novartis Pharmaceuticals, East Hanover, NJ (F.X., M.M., C.G., S.H.); and Novartis Pharma, Basel, Switzerland (F.S.).
Background: The inhibition of cyclin-dependent kinases 4 and 6 (CDK4/6) could potentially overcome or delay resistance to endocrine therapy in advanced breast cancer that is positive for hormone receptor (HR) and negative for human epidermal growth factor receptor 2 (HER2).
Methods: In this randomized, placebo-controlled, phase 3 trial, we evaluated the efficacy and safety of the selective CDK4/6 inhibitor ribociclib combined with letrozole for first-line treatment in 668 postmenopausal women with HR-positive, HER2-negative recurrent or metastatic breast cancer who had not received previous systemic therapy for advanced disease. We randomly assigned the patients to receive either ribociclib (600 mg per day on a 3-weeks-on, 1-week-off schedule) plus letrozole (2.
Effect of endovascular reperfusion in relation to site of arterial occlusion.
Neurology
February 2016
From KU Leuven, University of Leuven, Belgium, Department of Neurosciences, Experimental Neurology and Leuven Research Institute for Neuroscience and Disease (R.L.); University Hospitals Leuven, Department of Neurology (R.L.); VIB, Vesalius Research Center, Laboratory of Neurobiology (R.L.); Stanford Stroke Center (S.A.H., M.P.M., G.W.A., M.G.L.), Stanford University of Medicine, CA; Neurovascular Imaging Research Core and Department of Neurology and Comprehensive Stroke Center (D.S.L., J.L.S.), and Division of Interventional Neuroradiology, Department of Radiology, David Geffen School of Medicine (R.J.), University of California, Los Angeles; Departments of Radiology (T.A.T.) and Neurology (J.P.B.), University of Cincinnati Medical Center, OH; Departments of Clinical Neurosciences and Radiology (A.M.D.), Hotchkiss Brain Institute, Faculty of Medicine, University of Calgary, Canada; Departments of Neurology, Neurosurgery, and Radiology (R.G.N.), Emory University School of Medicine, Marcus Stroke and Neuroscience Center, Grady Memorial Hospital, Atlanta, GA; Department of Diagnostic and Interventional Neuroradiology (J.G.), University Hospital Bern, Switzerland; Division of Diagnostic and Interventional Neuroradiology (A.J.Y.), Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston; Department of Public Health Sciences (S.D.Y., Y.Y.P.), Medical University of South Carolina, Charleston; and Division of Neuroradiology, Department of Medical Imaging, and Division of Neurosurgery, Department of Surgery (V.M.P.), Toronto Western Hospital, University Health Network, Canada.
Objective: To assess whether the association between reperfusion and improved clinical outcomes after stroke differs depending on the site of the arterial occlusive lesion (AOL).
Methods: We pooled data from Solitaire With the Intention for Thrombectomy (SWIFT), Solitaire FR Thrombectomy for Acute Revascularisation (STAR), Diffusion and Perfusion Imaging Evaluation for Understanding Stroke Evolution Study 2 (DEFUSE 2), and Interventional Management of Stroke Trial (IMS III) to compare the strength of the associations between reperfusion and clinical outcomes in patients with internal carotid artery (ICA), proximal middle cerebral artery (MCA) (M1), and distal MCA (M2/3/4) occlusions.
Results: Among 710 included patients, the site of the AOL was the ICA in 161, the proximal MCA in 389, and the distal MCA in 160 patients (M2 = 131, M3 = 23, and M4 = 6).
Maize reas1 Mutant Stimulates Ribosome Use Efficiency and Triggers Distinct Transcriptional and Translational Responses.
Plant Physiol
February 2016
Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China (W.Q., J.Z., Q.Wu., Q.Wa., X.L., D.Y., Y.J., Ga.W., Gu.W., R.S.); and Coordinated Crop Biology Research Center, Beijing 100193, China (W.Q., Ga.W., Gu.W., R.S.) and National Maize Improvement Center of China, China Agricultural University, Beijing, 100193, China (R.S)
Ribosome biogenesis is a fundamental cellular process in all cells. Impaired ribosome biogenesis causes developmental defects; however, its molecular and cellular bases are not fully understood. We cloned a gene responsible for a maize (Zea mays) small seed mutant, dek* (for defective kernel), and found that it encodes Ribosome export associated1 (ZmReas1).
View Article and Find Full Text PDFPerivascular adipose adiponectin correlates with symptom status of patients undergoing carotid endarterectomy.
Stroke
June 2015
From the Departments of Surgery (G.S., M.T., K.D., W.K., A.L., C.K.O., M.E.S.) and Pathology (F.J.S.), Brigham and Women's Hospital/Harvard Medical School, Boston, MA; University of Washington School of Medicine, Seattle, WA (D.Y.); and Novartis Institutes for BioMedical Research, Cambridge, MA (G.D., X.W.).
Background And Purpose: Recent symptoms stand as a major determinant of stroke risk in patients with carotid stenosis, likely reflective of atherosclerotic plaque destabilization. In view of emerging links between vascular and adipose biology, we hypothesized that human perivascular adipose characteristics associate with carotid disease symptom status.
Methods: Clinical history, carotid plaques, blood, and subcutaneous and perivascular adipose tissues were prospectively collected from patients undergoing carotid endarterectomy.
Disabled-2 is required for efficient hemostasis and platelet activation by thrombin in mice.
Arterioscler Thromb Vasc Biol
November 2014
From the Graduate Institute of Biomedical Sciences (H.-J.T., Y.-W.C., C.-P.T.), and Department of Medical Biotechnology and Laboratory Science (C.-L.H., D.-Y.H., C.-C.L., C.-P.T.), College of Medicine, Chang Gung University, Taoyuan, Taiwan; Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA (J.A.C.); Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan (J.-C.C.); Molecular Medicine Research Center, Chang Gung University (C.-P.T.) and Department of Family Medicine (C.-P.T.), Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Objective: The essential role of platelet activation in hemostasis and thrombotic diseases focuses attention on unveiling the underlying intracellular signals of platelet activation. Disabled-2 (Dab2) has been implicated in platelet aggregation and in the control of clotting responses. However, there is not yet any in vivo study to provide direct evidence for the role of Dab2 in hemostasis and platelet activation.
View Article and Find Full Text PDFPrognostic value of blood interleukin-6 in the prediction of functional outcome after stroke: a systematic review and meta-analysis.
J Neuroimmunol
September 2014
Neurovascular Research Laboratory, Vall d'Hebron Institute of Research, Universitat Autònoma de Barcelona, Spain; Neurology Department, Hospital Vall d'Hebron, Barcelona, Spain. Electronic address:
We aimed to quantify the association of blood interleukin-6 (IL-6) concentrations with poor outcome after stroke and its added predictive value over clinical information. Meta-analysis of 24 studies confirmed this association with a weighted mean difference of 3.443 (1.
View Article and Find Full Text PDFc-Cbl inhibition improves cardiac function and survival in response to myocardial ischemia.
Circulation
May 2014
From the Cardiovascular Research Center and Department of Physiology, Temple University School of Medicine, Philadelphia, PA (K.R., M.A.K., R.S., J.G., X.G., Z.Q., D.Y., S.R.H., A. Sabri); Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha (B.M., N.Z., W.A., H.B.); and Department of Surgery, University of Connecticut Health Center, Farmington (A. Sanjay).
Background: The proto-oncogene Casitas b-lineage lymphoma (c-Cbl) is an adaptor protein with an intrinsic E3 ubiquitin ligase activity that targets receptor and nonreceptor tyrosine kinases, resulting in their ubiquitination and downregulation. However, the function of c-Cbl in the control of cardiac function is currently unknown. In this study, we examined the role of c-Cbl in myocyte death and cardiac function after myocardial ischemia.
View Article and Find Full Text PDFHuman IL-3/GM-CSF knock-in mice support human alveolar macrophage development and human immune responses in the lung.
Proc Natl Acad Sci U S A
February 2011
Department of Immunobiology, The Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520, USA.
Mice with a functional human immune system have the potential to allow in vivo studies of human infectious diseases and to enable vaccine testing. To this end, mice need to fully support the development of human immune cells, allow infection with human pathogens, and be capable of mounting effective human immune responses. A major limitation of humanized mice is the poor development and function of human myeloid cells and the absence of human immune responses at mucosal surfaces, such as the lung.
View Article and Find Full Text PDFNaive CD8+ T cells differentiate into protective memory-like cells after IL-2 anti IL-2 complex treatment in vivo.
J Exp Med
August 2007
Department of Immunology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.
An optimal CD8(+) T cell response requires signals from the T cell receptor (TCR), co-stimulatory molecules, and cytokines. In most cases, the relative contribution of these signals to CD8(+) T cell proliferation, accumulation, effector function, and differentiation to memory is unknown. Recent work (Boyman, O.
View Article and Find Full Text PDFExpression of hepatitis B surface antigen subtypes in liver of patients with hepatocellular carcinoma; comparison of subtypes in serum and liver.
Liver
June 1991
Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
To clarify the discrepancy in hepatitis B surface antigen (HBsAg) subtypes present in the serum and liver, as well as among hepatocytes, liver specimens which were resected from 37 HBsAg-positive patients with hepatocellular carcinoma (HCC) were examined. We evaluated HBsAg and the subtypic determinants of HBsAg and hepatitis B core antigen (HBcAg) using the peroxidase-antiperoxidase (PAP) staining method. Hepatitis B antigens were more frequently detected in small tumors (HBsAg in 67%.
View Article and Find Full Text PDF