FFR-Negative Nonculprit High-Risk Plaques and Clinical Outcomes in High-Risk Populations: An Individual Patient-Data Pooled Analysis From COMBINE (OCT-FFR) and PECTUS-obs.

Background: Despite fractional flow reserve (FFR)-guided deferral of revascularization, recurrent events in patients with diabetes or after myocardial infarction remain common. This study aimed to assess the association between FFR-negative but high-risk nonculprit lesions and clinical outcomes.

Methods: This is a patient-level pooled analysis of the prospective natural-history COMBINE (OCT-FFR) study (Optical Coherence Tomography Morphologic and Fractional Flow Reserve Assessment in Diabetes Mellitus Patients) and PECTUS-obs study (Identification of Risk Factors for Acute Coronary Events by OCT After STEMI and NSTEMI Patients With Residual Non- Flow Limiting Lesions).

Chemical shift encoding based double bonds quantification in triglycerides using deep image prior.

Fatty acid can potentially serve as biomarker for evaluating metabolic disorder and inflammation condition, and quantifying the double bonds is the key for revealing fatty acid information. This study presents an assessment of a deep learning approach utilizing deep image prior (DIP) for the quantification of double bonds and methylene-interrupted double bonds of triglyceride derived from chemical-shift encoded multi-echo gradient echo images, all achieved without the necessity for network training. The methodology implemented a cost function grounded in signal constraints to continually refine the neural network's parameters on a single slice of images through iterative processes.

A novel micelleplex for tumour-targeted delivery of CRISPR-Cas9 against KRAS-mutated lung cancer.

CRISPR-Cas9 has emerged as a highly effective and customizable genome editing tool, holding significant promise for the treatment of KRAS mutations in lung cancer. In this study, we introduce a novel micelleplex, named C14-PEI, designed to co-deliver Cas9 mRNA and sgRNA efficiently to excise the mutated KRAS allele in lung cancer cells. C14-PEI is synthesised from 1,2-epoxytetradecane and branched PEI 600 Da a ring-opening reaction.

Designing Short Cardin-Motif Peptide and Biopolymer-Based Multicomponent Hydrogels for Developing Advanced Composite Scaffolds for Improving Cellular Behavior.

Multicomponent self-assembly represents a cutting-edge strategy in peptide nanotechnology, enabling the creation of nanomaterials with enhanced physical and biological characteristics. This approach draws inspiration from the highly complex nature of the native extracellular matrix (ECM) constituting multicomponent biomolecular entities. In recent years, the combination of bioactive peptide with polymer has gained significant attention for the fabrication of novel biomaterials due to their inherent specificity, tunable physiochemical properties, biocompatibility, and biodegradability.

Dry crude pomegranate peel extract as a bio-input for medicinal pharmaceutical gel with healing activity.

The sustainable use of pomegranate peel, a by-product of the food industry, is gaining importance in developing pharmaceutical bio-inputs, aligning with circular economy practices and waste reduction. This study explores the application of dry crude pomegranate peel extract (PPE) as a bio-input for medicinal gels with wound healing properties. PPE was extracted via percolation in ethanol and freeze-dried.

Alopecia Management Potential of Rosemary-Based Nanoemulgel Loaded with Metformin: Approach Combining Active Essential Oil and Repurposed Drug.

Introduction: Androgenetic alopecia (AGA) is a multifactorial and age-related dermatological disease that affects both males and females, usually at older ages. Traditional hair repair drugs exemplified by minoxidil have limitations such as skin irritation and hypertrichosis. Thus, attention has been shifted to the use of repurposing drugs.

Soy protein-gellan gum noncovalent complexes stabilized emulsion: Effect of heating and pH on emulsion stability.

This paper investigated the effects of heating and pH on the stability of emulsions of non-covalent complexes of gellan gum (GG) and soy protein isolate (SPI). As a result, the GG-SPI complexes stabilized emulsion exhibited a minimum emulsion particle size (945 ± 23 nm), a maximum absolute values of zeta-potential (-32.7 ± 0.

Simultaneous extraction and purification of polysaccharides and proteins from Pleurotus ostreatus using an aqueous two-phase system.

Pleurotus ostreatus is a nutrient-dense edible fungus renowned for its delicate texture, appealing flavor, and numerous potential health benefits. Simultaneous extraction within the framework of food resource processing facilitates the concurrent isolation and analysis of multiple target compounds. In this study, an ethanol/salt aqueous two-phase system (ATPS) was employed to extract polysaccharides (PS) and proteins from P.

4-O-Methylglucaric Acid Production from Xylan with Uronic Acid Oxidase and Comparison to Glucaric Acid from Glucose.

This study describes an enzymatic pathway to produce high purity 4-O-methylglucaric acid from xylan, an underutilized fraction of lignocellulosic biomass. Beechwood xylan was enzymatically hydrolysed using a commercial xylanase and an α-glucuronidase from Amphibacillus xylanus to form 4-O-methylglucuronic acid, which was then purified by anion exchange chromatography and subsequently oxidized to 4-O-methylglucaric acid using a recombinantly produced uronic acid oxidase from Citrus sinensis. Enzymatic oxidation with uronic acid oxidase afforded 95 % yield in 72 hours which is considerably higher than yields previously achieved using a glucooligosaccharide oxidase from Sarocladium strictum.

The biomechanical effect of the O-A angle on the aortic valve under left ventricular assist device support: a primary fluid-structure interaction study.

Background: Left ventricular assist device (LVAD) has been widely used as an alternative treatment for heart failure, however, aortic regurgitation is a common complication in patients with LVAD support. And the O-A angle (the angle between LVAD outflow graft and the aorta) is considered as a vital factor associated with the function of aortic valve. To date, the biomechanical effect of the O-A angle on the aortic valve remains largely unknown.

Thoracoscopic anatomical sublobar resection for deep interlobar lung cancer with fused fissure.

Background: The technical challenges associated with the removal of small nodules in challenging locations rather than peripheral locations remain unaddressed. We sought to illustrate the parenchymal-sparing surgical approach employed for deep interlobar lung cancer with fused fissures (DILCFFs).

Methods: A retrospective review of 43 patients with cT1N0M0 DILCFFs from January 2013 through December 2022 was performed.

Maleic Acid-Butanol Pretreatment to Enhance Cellulose Accessibility for Enzymatic Hydrolysis and Ethanol Production from Oil Palm Empty Fruit Bunch.

Pretreatment of lignocellulosic biomass is crucial yet challenging for sustainable energy production. This study focuses on enhancing enzymatic accessibility of cellulose in oil palm empty fruit bunches by optimizing pretreatment parameters to improve glucose and ethanol yields while reducing fermentation inhibitors. It evaluates the impact of maleic acid concentrations on biorefinery processes.

Bis-pyrrolidone structures as versatile building blocks for the synthesis of bio-based polyesters and for the preparation of additives.

In this work, three bis-pyrrolidone-based structures (BP) were synthesized combining dimethyl itaconate (DMI), the dimethyl ester derivative of itaconic acid, with various aliphatic diamines having a C4 to C12 carbon chain length with the aim of developing novel bio-based building blocks. All three BPs were obtained with a purity >93% and could further be used without performing any tedious purification step, therefore allowing an easy scalability of the synthesis on a 10 g scale. Their potential application was demonstrated in two key areas of modern polymer science: (1) the enzymatic synthesis of polyesters and (2) their use as poly(lactic acid) (PLA) additives.

Analytical and Applied Copyrolysis of L. and Polyethyelene Terephthalate Plastic for Hydrocarbon-Rich Bio-oil.

The current investigation focuses on the copyrolysis of L. (a nonedible oilseed, also known as Nahar) and polyethyelene terephthalate (PET) plastic waste to gain insights into the composition of pyrolysates and the thermal decomposition of complex and mixed feedstocks. The physicochemical properties of the feedstocks were studied through thermogravimetric analysis at a heating rate of 15 °C min, bomb calorimetry, and proximate/ultimate analysis.

Predicting right ventricular failure after left ventricular assist device implant: A novel approach.

Aims: Right ventricular (RV) failure (RVF) after left ventricular assist device (LVAD) implant is an important cause of morbidity and mortality. Modern, data-driven approaches for defining and predicting RVF have been under-utilized.

Methods: Two hundred thirty-two patients were identified with a mean age of 55 years; 40 (17%) were women, 132 were (59%) Caucasian and 74 (32%) were Black.

Evaluating the properties of starch/chitosan films with the incorporation of various nanoclays for use in food packaging.

This study evaluates the properties of starch/chitosan films (SCF) produced via the casting method, incorporating 40 % (w/w) plasticizers (glycerol and sorbitol) and various concentrations (0, 3, 5, and 10 % (w/w)) of nanoclays (Cloisite 20A, Cloisite 30B, and K-10). The effects of each nanofiller on the films were thoroughly investigated. Films containing nanoclays exhibited reduced water solubility and enhanced thermal stability compared to films without nanofillers.

Selective deficiency of mitochondrial respiratory complex I subunits Ndufs4/6 causes tumor immunogenicity.

Cancer cells frequently rewire their metabolism to support proliferation and evade immune surveillance, but little is known about metabolic targets that could increase immune surveillance. Here we show a specific means of mitochondrial respiratory complex I (CI) inhibition that improves tumor immunogenicity and sensitivity to immune checkpoint blockade (ICB). Targeted genetic deletion of either Ndufs4 or Ndufs6, but not other CI subunits, induces an immune-dependent growth attenuation in melanoma and breast cancer models.

Encapsulation of black soldier fly larvae oil in zein ultrafine fibers via electrospinning: Characterization and antioxidant properties.

Black soldier fly larvae (BSFL) have several advantages, such as rapid growth and sustainable production. The electrospinning encapsulation shows promise for encapsulating oils, as it does not use high temperatures, preventing degradation. This study analyzed the incorporation of oil (15, 30, and 45 % w/w) from BSFL into fibers by electrospinning using zein (20 and 25 % w/v).

A New and Safe Delivery of Sildenafil Citrate Co-Evaporate Loaded Emulgels for the Cure of Certain Male Sexual Dysfunctions.

The present work focuses on the production of sildenafil co-evaporates loaded emulgels as topical dosage forms for the treatment of premature ejaculation and erectile dysfunction. Topical administration of sildenafil citrate (SILD) co-evaporates is expected to improve the bioavailability profile of the drug and to avoid the severe side effects accompanying the traditional SILD dosage forms, especially for prohibited cardiovascular cases. Firstly, the solubility of SILD was improved via solid dispersion via co-evaporation technique using PEG-5KDa and PVP-K90 as hydrophilic carriers.

High-resolution, noninvasive single-cell lineage tracing in mice and humans based on DNA methylation epimutations.

In vivo lineage tracing holds great potential to reveal fundamental principles of tissue development and homeostasis. However, current lineage tracing in humans relies on extremely rare somatic mutations, which has limited temporal resolution and lineage accuracy. Here, we developed a generic lineage-tracing tool based on frequent epimutations on DNA methylation, enabled by our computational method MethylTree.

The impact of glidants on the rheological properties of active pharmaceutical ingredients: A study of conventional and alternative flow enhancers.

Nowadays, most of the newly developed active pharmaceutical ingredients (APIs) consist of cohesive particles with a mean particle size of <100μm, a wide particle size distribution (PSD) and a tendency to agglomerate, therefore they are difficult to handle in continuous manufacturing (CM) lines. The current paper focuses on the impact of various glidants on the bulk properties of difficult-to-handle APIs. Three challenging powders were included: two extremely cohesive APIs (acetaminophen micronized (APAPμ) and metoprolol tartrate (MPT)) which previously have shown processing issues during different stages of the continuous direct compression (CDC)-line and a spray dried placebo (SD) powder containing hydroxypropylmethyl cellulose (HPMC), known for its sub-optimal flow with a high specific surface area (SSA) and low density.

Techno-functionality of jack bean (Canavalia ensiformis) protein concentrate: a comparative study with soy and pea proteins.

Background: With the growing human awareness of the environmental and animal stress caused by the meat industry, the consumption of plant-based products has expanded. Plant proteins have gained market prominence due to their sustainable origin, economic value and health benefits. Well-established plant proteins in the market, such as those of soy and pea, have various applications as ingredients in the food industry.

Dual-protein system composed of soy protein and β-lactoglobulin: effect of transglutaminase-mediated crosslinking on its allergenicity and conformation.

Background: The escalating global prevalence of food allergies has intensified the need for hypoallergenic food products. Transglutaminase (TGase)-mediated crosslinking has garnered significant attention for its potential to reduce the allergenicity of food proteins. This study aimed to investigate the effects of TGase crosslinking on the potential allergenicity and conformational changes in a dual-protein system composed of β-lactoglobulin (β-LG) and soy protein isolate (SPI) at varying mass ratios (10:0, 7:3, 5:5, 3:7 and 0:10 (w/w)).

Drug Delivery Applications of Hydrophobic Deep Eutectic Solvent-in-Water Nanoemulsions: A Comparative Analysis of Ultrasound Emulsification and Membrane-Assisted Nanoemulsification.

The emergence of green chemistry and engineering principles to enforce sustainability aspects has ensured the prevalence of green solvents and green processes. Our study addresses this quest by exploring drug delivery applications of hydrophobic deep eutectic solvents (DESs) which are alternative green solvents. Initially, this work showcases the hydrophobic drug solubilization capabilities of a natural hydrophobic DES, menthol, and decanoic acid.

Development of an antimicrobial tissue conditioner with quaternary ammonium methacryloxy silane (K18): An in vitro study.

Purpose: To determine the effects of K18 quaternary ammonium methacryloxy silane (QAS) on tissue conditioner materials and their antimicrobial properties.

Methods: 30% K18 QAS in methyl methacrylate (MMA; K18-MMA; 0%, 15%, and 20% w/w) was incorporated into a commercial tissue conditioner (Coe comfort). The degree of curing (Shore A hardness), hydrophilicity (contact angle), flow, liquid sorption, mass loss, and antimicrobial properties of Streptococcus mutans, Streptococcus sanguinis, and Candida albicans were determined.