Impact of fresh-cut onion types and flavonoid profiles on the survival kinetics of foodborne pathogen during refrigerated storage.

This study investigated the effect of onion flavonoid profiles on the growth, survival, and/or death kinetics of foodborne pathogens in fresh-cut onions at 4 °C. Fresh-cut white, yellow, red, and sweet onions were inoculated with separate four-strain cocktail(s) of nalidixic acid-adapted Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes achieving a 4 to 5 log CFU g.

Structural and functional alterations in hypothalamic subregions in male patients with alcohol use disorder.

Background: The hypothalamus is involved in stress regulation and reward processing, with its various nuclei exhibiting unique functions and connections. However, human neuroimaging studies on the hypothalamic subregions are limited in drug addiction. This study examined the volumes and functional connectivity of hypothalamic subregions in individuals with alcohol use disorder (AUD).

A Preliminary Analysis of a Prosocial Intervention to Support Teachers and Staff Implementing Behavioral Interventions in a Specialized School Setting.

Prosocial is a systems-level intervention framework that incorporates elements of acceptance and commitment therapy (ACT) with Ostrom's core design principles (CDPs) guiding effective organizations and collective action (Ostrom, 1990). We evaluated a Prosocial intervention to support teachers and staff in a specialized school. AB analysis supported improvements in group cohesion and values-behavior alignment during the intervention and pre-post analyses showed significant improvements in psychological flexibility and perceived stress of participants.

Surface interactions of gelatin-sourced carbon quantum dots with a model globular protein: insights into carbon-based nanomaterials and biological systems.

Carbon nanomaterials (CNMs), such as carbon nanotubes (CNTs), graphene quantum dots (GQDs), and carbon quantum dots (CQDs), are prevalent in biological systems and have been widely utilized in applications like environmental sensing and biomedical fields. While their presence in human matrices is projected to increase, the interfacial interactions between carbon-based nanoscopic platforms and biomolecular systems continue to remain underexplored. In this study, we investigated the effect of gelatin-sourced CQDs on the globular milk protein beta-lactoglobulin (BLG).

Context familiarity is a third kind of episodic memory distinct from item familiarity and recollection.

Episodic memory is accounted for with two processes: "familiarity" when generally recognizing an item and "recollection" when retrieving the full contextual details bound with the item. We tested a combination of item recognition confidence and source memory, focusing upon three conditions: "item-only hits with source unknown" ('item familiarity'), "low-confidence hits with correct source memory" ('context familiarity'), and "high-confidence hits with correct source memory" ('recollection'). Behaviorally, context familiarity was slower than the others during item recognition, but faster during source memory.

Dissecting the role of vitamin B metabolism in craniofacial development through analysis of clinical phenotypes and model organism discoveries.

Vitamin B, otherwise known as cobalamin, is an essential water-soluble vitamin that is obtained from animal derived dietary sources. Mutations in the genes that encode proteins responsible for cobalamin uptake, transport, or processing cause inborn errors of cobalamin metabolism, a group of disorders characterized by accumulation of homocysteine and methylmalonic acid, neurodevelopmental defects, ocular dysfunction, anemia, and failure to thrive. Mild to moderate craniofacial phenotypes have been observed but these phenotypes are not completely penetrant and have not been consistently recognized in the literature.

Variations in brain glutamate and glutamine levels throughout the sleep-wake cycle.

Background: Glutamatergic signaling is essential for modulating synaptic plasticity and cognition. However, the dynamics of glutamatergic activity over the 24-hour sleep-wake cycle, particularly in relation to sleep, remain poorly understood. This study aims to investigate diurnal variations in brain Glx levels-representing the combined concentrations of glutamate and glutamine-in humans and to explore their implications for cognitive performance and sleep pressure.

Cardiovascular reactivity during conversations about discrimination is buffered by social support among U.S. Latines.

Racial discrimination is conceptualized as an acute and chronic stressor. Like other acute stressors, lab-based studies demonstrate acute effects of discrimination-related stressors on stress-related cardiovascular outcomes, including total cardiac output, blood pressure, and indices of sympathetic and parasympathetic nervous system activity. Critically, it is important to understand how individual and social factors buffer the experience of race-related acute stress.

Distinct populations suppress or escalate intake of cocaine paired with aversive quinine.

Background: Only a subset of individuals who encounter illicit drugs become persons with a substance use disorder. Individual differences in aversive reactions to drug-associated phenomena like smoke inhalation and unpleasant taste are predictors for continued use. While several preclinical studies have explored self-administration involving aversive cues, none have simultaneously introduced aversion with the initial drug self-administration.

Exploring predictors of COVID-19 precautionary behaviors during the initial rollout of COVID-19 vaccines in a predominately Hispanic sample.

•Precautionary behaviors were lower if primary language in household was Spanish.•Testing positive for COVID-19 was inversely associated with precautionary behaviors.•Healthcare practitioners were the most trusted source of COVID-19 information.

Don't worry, be APPE: Developing and refining a skills-based assessment of student preparedness for advanced pharmacy practice experiences.

Background: The Accreditation Council for Pharmacy Education (ACPE) 2025 Standards charge pharmacy programs to assess student readiness for Advanced Pharmacy Practice Experiences (APPEs), but the method of assessment is not defined. This report describes the process of developing, implementing, and refining an APPE readiness assessment (ARA) that can be adapted to individual institutional needs and learning outcomes.

Educational Activity: The ARA at The University of Texas at El Paso School of Pharmacy, a Hispanic-serving institution, is comprised of two practice areas: drug information and the Pharmacist Patient Care Process, each with multiple objective structured clinical examinations (OSCEs) to mimic how students will apply these skills in required APPE rotations.

Process parameter translation strategies for variable directed energy deposition spot size using 316L, copper, and Inconel 625.

Directed energy deposition (DED) is a form of additive manufacturing available across a variety of laser spot diameter values, often referred to as spot sizes. However, there is no method to easily transfer process parameters across discrete spot sizes, leading to DED process parameters that are equipment specific and not widely applicable. In this study, a strategy is proposed and investigated for five spot sizes that keep the areal energy density constant while varying power, feed rate, and powder flow during the deposition of 316L stainless steel.

Rodent competition and fire alter patterns of mound and disk formation of western harvester ants.

Consumers exert top-down controls on dryland ecosystem function, but recent increases in fire activity may alter consumer communities in post-fire environments. Native consumers, including ants and rodents, likely have critical roles in defining post-fire plant community assembly and resilience to biological invasions. This study aimed to understand how western harvester ants (Pogonomyrmex occidentalis) that form mounds and large vegetation-free disks that significantly influence plant community structure in the Great Basin Desert respond to fire and rodent community abundance.

A six degrees of freedom femtosecond laser system for fabrication of small-scale mechanical property specimens.

We present the details of a novel ultra-short pulsed laser machining workstation that has been employed for high-throughput laser machining of small-scale mechanical property specimens. This system employs a six degrees of freedom hexapod positioning stage capable of macroscopic movements at high positional accuracy. We developed a methodology that uses quantitative image analysis to measure key parameters required to minimize the hexapod positioning and rotational error.

Fibrin-Polycaprolactone Scaffolds for the Differentiation of Human Neural Progenitor Cells into Dopaminergic Neurons.

Parkinson's disease (PD), a progressive central nervous system disorder marked by involuntary movements, poses a significant challenge in neurodegenerative research due to the gradual degeneration of dopaminergic (DA) neurons. Early diagnosis and understanding of PD's pathogenesis could slow disease progression and improve patient management. In vitro modeling with DA neurons derived from human-induced pluripotent stem cell-derived neural progenitor cells (NPCs) offers a promising approach.

An approximate solution of the SLIP model under the regime of linear angular dynamics during stance and the stability of symmetric periodic running gaits.

Terrestrial locomotion is a complex phenomenon that is often linked to the survival of an individual and of an animal species. Mathematical models seek to express in quantitative terms how animals move, but this is challenging because the ways in which the nervous and musculoskeletal systems interact to produce body movement is not completely understood. Models with many variables tend to lack biological interpretability and describe the motion of an animal with too many independent degrees of freedom.

Testing Protocol Development for the Fracture Toughness of Parts Built with Big Area Additive Manufacturing.

The mechanical testing of additively manufactured parts has largely relied on the existing standards developed for traditional manufacturing. While this approach leverages the investment made in current standards development, it inaccurately assumes that the mechanical response of additive manufacturing (AM) parts is identical to that of parts manufactured through traditional processes. When considering thermoplastic, material extrusion AM, the differences in response can be attributed to an AM part's inherent inhomogeneity caused by porosity, interlayer zones, and surface texture.

False memories in bilinguals: Integration of information across languages and limits on proficiency effects.

Research on false memory in bilinguals using the DRM task has shown that false memories transfer across languages, but comparisons to within-language conditions have yielded mixed results. In two experiments, Spanish-English bilinguals completed standardized language assessments and a DRM task. Experiment 1 (N = 96) had several study-recall cycles before a final recognition test, and Experiment 2 (N = 72) only tested recognition.

Reliability assessment of leg length and angular alignment on manual reads versus artificial intelligence-generated lower extremity radiographic measurements.

Purpose: Leg length discrepancy (LLD) and lower extremity malalignment can lead to pain and osteoarthritis. A variety of radiographic parameters are used to assess LLD and alignment. A 510(k) FDA approved artificial intelligence (AI) software locates landmarks on full leg standing radiographs and performs several measurements.

Synthesis of a new series of 4-pyrazolylquinolinones with apoptotic antiproliferative effects as dual EGFR/BRAF inhibitors.

The current study focuses on developing a single molecule that acts as an antiproliferative agent with dual or multi-targeted action, reducing drug resistance and adverse effects. A new series of 4-pyrazolylquinolin-2-ones (5a-j) with apoptotic antiproliferative effects as dual EGFR/BRAF inhibitors were designed and synthesized. Compounds 5a-j were investigated for their cell viability effect against a normal cell line (MCF-10A).

Distinct populations suppress or escalate intake of cocaine paired with aversive quinine.

Background: Only a subset of individuals who encounter drugs of abuse become habitual users. Aversive subjective effects like coughing and unpleasant taste are predictors for continued use. While several preclinical studies have explored self-administration involving aversive cues, none have simultaneously introduced aversion with the initial drug self-administration.

The enigma of fine mobile structures on the aortic surface in a patient undergoing transcatheter aortic valve replacement: a case report.

Background: The surface of the aorta generally does not show motion unless mobile atheroma, thrombi, vegetations, or intimal flaps are present. We previously described unusual mobile filamentous structures in the carotid artery. Here, we describe similar findings in the aorta and their possible cause.

Ultrafast Detection of Arsenic Using Carbon-Fiber Microelectrodes and Fast-Scan Cyclic Voltammetry.

Arsenic contamination poses a significant public health risk worldwide, with chronic exposure leading to various health issues. Detecting and monitoring arsenic exposure accurately remains challenging, necessitating the development of sensitive detection methods. In this study, we introduce a novel approach using fast-scan cyclic voltammetry (FSCV) coupled with carbon-fiber microelectrodes (CFMs) for the electrochemical detection of As.