Interactions between glucagon like peptide 1 (GLP-1) and estrogens regulates lipid metabolism.

Obesity, characterized by excessive fat accumulation in white adipose tissue (WAT), is linked to numerous health issues, including insulin resistance (IR), and type 2 diabetes mellitus (DM2). The distribution of adipose tissue differs by sex, with men typically exhibiting android adiposity and pre-menopausal women displaying gynecoid adiposity. After menopause, women have an increased risk of developing android-type obesity, IR, and DM2.

Mycophenolate Mofetil-Induced Lymphoproliferative Disorder in a Young Adult With Chronic Posterior Uveitis.

To present a case of a young adult with bilateral chronic posterior uveitis resulting from Vogt-Koyanagi-Harada (VKH) disease treated with mycophenolate mofetil who subsequently developed vitreoretinal lymphoma in the right eye. A case and its findings were analyzed. A 34-year-old Hispanic woman presented with an 8-year history of chronic recurrent posterior uveitis in both eyes secondary to VKH disease.

Adaptive multi-beam X-ray ptychography.

Ptychography has evolved as an important method for nanoscale X-ray imaging with synchrotron radiation. Recently, it has been proposed to work with multiple beams in parallel. The main advantage of so-called multi-beam ptychography is that larger areas can be imaged much faster than with a conventional single beam scan.

Histological, histochemical, and immunohistochemical characterization of NANOULCOR nanostructured fibrin-agarose human cornea substitutes generated by tissue engineering.

Background: Human artificial corneas (HAC) generated by tissue engineering recently demonstrated clinical usefulness in the management of complex corneal diseases. However, the biological mechanisms associated to their regenerative potential need to be elucidated.

Methods: In the present work, we generated HAC using nanostructured fibrin-agarose biomaterials with cultured corneal epithelial and stromal cells, and we compared the structure and histochemical and immunohistochemical profiles of HAC with control native corneas (CTR-C) and limbus (CTR-L) to determine the level of biomimicry of the HAC with these two native organs.

Procollagen-lysine 2-oxoglutarate 5-dioxygenases are responsible for 5R-hydroxylysine modification of therapeutic T-cell bispecific monoclonal antibodies produced by Chinese hamster ovary cells.

We present a detailed mass spectrometric analysis of three 2 + 1 T-cell bispecific monoclonal antibodies (TCB mAbs), where an unexpected +15.9950 Da mass shift in tryptic peptides was observed. This modification was attributed to the occurrence of 5R-hydroxylysine (Hyl) using a hybrid LC-MS/MS molecular characterization and CRISPR/Cas9 gene deletion approach.

Longitudinal effects of SARS-CoV-2 breakthrough infection on imprinting of neutralizing antibody responses.

Background: The impact of the infecting SARS-CoV-2 variant of concern (VOC) and the vaccination status was determined on the magnitude, breadth, and durability of the neutralizing antibody (nAb) profile in a longitudinal multicentre cohort study.

Methods: 173 vaccinated and 56 non-vaccinated individuals were enrolled after SARS-CoV-2 Alpha, Delta, or Omicron infection and visited four times within 6 months and nAbs were measured for D614G, Alpha, Delta, BA.1, BA.

Dairy cows' responses to 2 separation methods after 3 months of cow-calf contact.

Weaning and separation remain among some of the biggest challenges for cow-calf contact systems, making the development of practical and low-stress separation methods mandatory for future success of these systems. This study aimed to compare behavioral and physiological responses of dairy cows to separation from their calves after 3 mo of full-time contact, with either the 2-step method (NF, 2 wks full-time contact while calves wore a nose flap, 1 wk fence-line contact before total separation, n = 18) or by gradual reduction of contact time between cow and calf (GR, 1 wk half day contact, 1 wk morning contact, 1 wk fence-line contact before total separation, n = 18). Vocalizations and searching behavior were observed on 4 d/wk from 1 wk before separation until 1 wk after total separation.

Suprathel® and water-filtered infrared-A radiation (wIRA) as a new treatment strategy for toxic epidermal necrolysis (TEN): A prospective study.

Toxic epidermal necrolysis (TEN) is a life-threatening condition with a mortality rate of approximately 25 % to 30 %. Early and adequate wound coverage is necessary due to large skin defects. Suprathel® is a modern wound dressing that shows promising results when treating superficial wounds such as scalds, burns and abrasions.

Screening, brief intervention, and referral to treatment for pain management for veterans separating from military service: study protocol of a hybrid type 2 study testing implementation facilitation versus training-as-usual.

Background: Veterans transitioning to civilian life often have chronic pain from service-related musculoskeletal disorders (MSD) with higher risk for substance misuse. Many seek VA (Department of Veterans Affairs) compensation for MSD. Use of Screening, Brief Intervention, and Referral to Treatment for Pain Management (SBIRT-PM) by VA Post-9/11 Military2VA (M2VA) case managers presents an opportunity to engage these veterans in VA pain care and address substance misuse.

Vogt-Koyanagi-Harada Disease Presenting as Papillitis vs. Classic Serous Retinal Detachment: A Comparative Analysis of Outcomes.

Background: To compare the treatment outcome and visual prognosis of Vogt-Koyanagi-Harada (VKH) disease presenting as papillitis vs. serous retinal detachment (SRD).

Methods: Retrospective comparative cohort study of 35 patients with previously unknown VKH diagnosis, followed-up for ≥6 months.

Measuring Patient Well-Being During Whole-Person Clinical Care: Lessons From the Veterans Health Administration Well-Beings Signs Implementation Pilot.

Background: Measuring patient well-being during clinical care may enhance patient-centered communication and treatment planning. The Veterans Health Administration (VHA) piloted the use of the Well-Being Signs (WBS), a self-report measure of psychosocial well-being, in clinical care.

Objective: To understand early WBS implementation and health care team member experiences with its use before developing detailed support materials and finalizing the measure.

Measuring the Well-Being of the Whole Person in Clinical Care, Health Research, and Population Health Evaluation: Findings and Recommendations From the XVIII Veterans Health Administration State of the Art Meeting.

Objectives: In 2023, the U.S. Veterans Health Administration convened a State of the Art meeting to evaluate the existing evidence and make recommendations for measuring the well-being of the whole person in clinical care, health research, and population health evaluation.

STRIVE pilot trial: a protocol for a multicentre pragmatic internal pilot randomised controlled trial of Structured TRaining to Improve fitness in a Virtual Environment (STRIVE) before surgery.

Introduction: Home-based, virtually-supported care models may represent the most efficient and scalable approach to delivering prehabilitation services. However, virtual approaches to prehabilitation are understudied. This manuscript describes the protocol for an internal pilot randomised controlled trial of a virtually-delivered, multimodal prehabilitation intervention.

Managing Vogt-Koyanagi-Harada disease during pregnancy with steroid pulse therapy: A case report.

Background: High-dose steroid administration is a common initial therapeutic approach for Vogt-Koyanagi-Harada disease (VKH). Nonetheless, administering substantial doses of steroids to pregnant women necessitates meticulous consideration due to the potential impacts on the mother and fetus. We present a case wherein steroid pulse therapy was administered to a patient who developed VKH during the late stages of pregnancy.

Substrate specificity and kinetic mechanism of 3β-hydroxy-Δ-C-steroid oxidoreductase.

Cholesterol is a key sterol whose homeostasis is primarily maintained through bile acid metabolism. Proper bile acid formation is vital for nutrient and fat-soluble vitamin absorption and emulsification of lipids. Synthesis of bile acids occurs through two main pathways, both of which rely on 3β-hydroxy-Δ-C-steroid oxidoreductase (HSD3B7) to begin epimerization of the 3β hydroxyl of cholesterol into its active 3α conformation.

A case of unilateral focal choroidal excavation in Vogt-Koyanagi-Harada disease; case report.

Purpose: To report a rare case of Vogt-Koyanagi-Harada (VKH) disease complicated by unilateral focal choroidal excavation (FCE).

Methods: A 25-year-old male patient with bilateral blurred vision, tinnitus, and headache. Initial examination revealed multiple sensory retinal detachments in both eyes.

How well do different COVID-19 vaccines protect against different viral variants? A systematic review and meta-analysis.

While the efficacy of coronavirus disease 2019 (COVID-19) vaccines has been evaluated in numerous trials, comprehensive evidence on how protection by different vaccines has varied over time remains limited. We aimed to compare protective effects of different vaccines against different viral variants. To achieve this, we searched Medline, Cochrane Library and Embase for randomized controlled trials assessing the efficacy of COVID-19 vaccines.