1 results match your criteria: "Virus Laboratory of Veterinary Institute[Affiliation]"
[Expression of anti-HIV envelope glycoprotein scFv in Pichia pastoris and its biologic activities analysis].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
December 2007
Virus Laboratory of Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062, China.
Aim: To construct the vector and express anti-HIV-1 envelope glycoprotein single chain Fv fragment in Pichia pastoris.
Methods: The target gene was digested from plasmid pET28-scFv and cloned into pichia pastoris vector via gene engineering and DNA recombination techniques. The recombinant plasmid was linearized and transferred into Pichia pastoris strains GS115 by electroporation.