Expressing red fluorescent protein on the surface of using C-terminal domain of autotransporters.

The Type V secretion system, or "autotransporter", is a secretion system that enables bacteria to directly export proteins from the cell interior to the extracellular membrane. mCherry is a second-generation monomeric red fluorescent protein that has an improvement in photostability compared to the first generation of RFP. In this research, we conducted the fusion of the mRFP into the C-terminal domain of EhaA - the translocation domain of the autotransporter protein transport system - to investigate the expression of mRFP on the surface of a model organism commonly utilized in recombinant protein research.

A comparative analysis of Marburg virus-infected bat and human models from public high-throughput sequencing data.

Marburg virus (MARV) disease (MVD) is an uncommon yet serious viral hemorrhagic fever that impacts humans and non-human primates. In humans, infection by the MARV is marked by rapid onset, high transmissibility, and elevated mortality rates, presenting considerable obstacles to the development of vaccines and treatments. Bats, particularly , are suspected to be natural hosts of MARV.

Results of treating patients with cellulitis by hyperbaric oxygen: a case-control study.

Background: Cellulitis is an acute bacterial infection of the skin and subcutaneous tissue. The characteristic symptoms of the disease are an area of skin that is swollen, warmth, redness, pain, unknown borderline, tends to expand, and can lead to tissue necrosis. Hyperbaric Oxygen (HBO) is a non-drug treatment, which has the effect of relieving pain, reducing edema, and accelerating the wound healing process for patients with cellulitis.

Neonatal sepsis in Vietnam: Bacterial profiles and antibiotic susceptibility in a tertiary care setting.

Background: Neonatal sepsis is a leading cause of newborn mortality, particularly in low and middle-income countries. This study examines the bacterial etiologies and antibiotic resistance patterns of neonatal sepsis in a tertiary hospital in Vietnam.

Methods: A prospective cross-sectional study was conducted at National Children's Hospital, Hanoi, Vietnam from January 2021 to December 2022.

Electroacupuncture at Traditional Acupoints or Myofascial Trigger Points for Chronic Nonspecific Low Back Pain: High or Alternated Frequency? A Double-Blinded Randomized Controlled Trial.

Background: Chronic nonspecific low back pain (cNLBP) can be effectively treated by electroacupuncture (EA) at traditional acupoints (TAPs) and myofascial trigger points (MTrPs). However, the optimal type and frequency of stimulation (alternated frequency [AF] and high frequency [HF]) remain unclear. This study aimed to explore this.

Foot and Ankle Reconstruction Using Retrograde Lateral Supramalleolar Flap.

 Treatment of soft-tissue defects in the foot and ankle is a challenge. The use of a retrograde lateral supramalleolar flap is a promising technique for reconstruction. This study aims to assess the efficacy and outcomes of the technique.

Alanine to glycine substitution in the PyR2 confers sodium channel resistance to Type I pyrethroids.

Background: Aedes aegypti is a primary urban vector of dengue, yellow fever, Zika and chikungunya worldwide. Pyrethroid insecticides are the most effective insecticides for controlling Ae. aegypti.

Enhanced heart sound classification using Mel frequency cepstral coefficients and comparative analysis of single vs. ensemble classifier strategies.

This paper seeks to enhance the performance of Mel Frequency Cepstral Coefficients (MFCCs) for detecting abnormal heart sounds. Heart sounds are first pre-processed to remove noise and then segmented into S1, systole, S2, and diastole intervals, with thirteen MFCCs estimated from each segment, yielding 52 MFCCs per beat. Finally, MFCCs are used for heart sound classification.

A primary school-based dengue solution model for post-COVID-19 in southern Thailand: Students understanding of the dengue solution and larval indices surveillance system.

One of the consequences of the COVID-19 lockdown is that it hinders school-based dengue management interventions. This is due to the closure of schools and the limited availability of online lessons in certain schools. Conversely, the level of basic understanding that primary school children have about the condition is directly related to their likelihood of getting it and their ability to modify their behaviour to prevent it.

Factors associated with retention in Quitline counseling for smoking cessation among HIV-positive smokers receiving care at HIV outpatient clinics in Vietnam.

Background: Quitline counseling is an effective method for supporting smoking cessation, offering personalized and accessible assistance. Tobacco use is a significant public health issue among people living with HIV. In Vietnam, over 50% of men living with HIV use tobacco.

Maximizing nutrient removal: unveiling the influence of biomass retention time in revolving algae biofilm reactor.

This study introduces a novel Revolving Algae Biofilm reactor for synthetic wastewater treatment, examining the influence of various biomass retention times (BRTs) on nutrient removal performance. The study reveals complex interactions between microalgae and bacteria, emphasizing their symbiotic functions in oxygen provision, nutrient absorption, and floc creation. This research contributes to the advancement of sustainable wastewater treatment methods, showing promise for large-scale nutrient removal in industrial settings.

A multi-channel bioimpedance-based device for Vietnamese hand gesture recognition.

This study addresses the growing importance of hand gesture recognition across diverse fields, such as industry, education, and healthcare, targeting the often-neglected needs of the deaf and dumb community. The primary objective is to improve communication between individuals, thereby enhancing the overall quality of life, particularly in the context of advanced healthcare. This paper presents a novel approach for real-time hand gesture recognition using bio-impedance techniques.

Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability.

For over a century African swine fever (ASF) has been causing outbreaks leading to devastating losses for the swine industry. The current pandemic of ASF has shown no signs of stopping and continues to spread causing outbreaks in additional countries. Currently control relies mostly on culling infected farms, and strict biosecurity procedures.

NET formation-mediated in situ protein delivery to the inflamed central nervous system.

Delivering protein drugs to the central nervous system (CNS) is challenging due to the blood-brain and blood-spinal cord barrier. Here we show that neutrophils, which naturally migrate through these barriers to inflamed CNS sites and release neutrophil extracellular traps (NETs), can be leveraged for therapeutic delivery. Tannic acid nanoparticles tethered with anti-Ly6G antibody and interferon-β (aLy6G-IFNβ@TLP) are constructed for targeted neutrophil delivery.

Radiological features of basal ganglia germinoma: a case report and early-stage alerts.

Basal ganglia germinomas are uncommon neoplasms. Basal ganglia germinomas exhibit high sensitivity to both radiation therapy and chemotherapy. In contrast, surgery is the standard treatment for most primary brain tumors (such as gliomas, which are the most common tumors in the pediatric basal ganglia region).

Dataset on ITS and some chloroplast DNA regions of Blume in Vietnam.

Species of the genus have the potential to be natural medicines and have industrial fibre production uses. Many species of this genus are morphologically similar and are difficult to distinguish, especially when their morphology is distorted. This dataset includes sequence information of several DNA regions isolated from the genome of , namely ITS (from the nuclear genome), , trnL-trnF, trnH-psbA, and (from the chloroplast genome) and phylogenetic analysis results based on the isolated sequences.

Management of cardiac trauma and penetrating cardiac injuries with severe hemorrhagic shock: a 5-year experience.

Purpose: The diagnosis and management of cardiac trauma and penetrating cardiac injuries pose significant challenges in emergency settings due to the rapid onset of life-threatening complications. This paper presents a narrative review to better describe the etiology, presentation, and management of penetrating cardiac trauma, offering insights and experiences related to performing emergency surgery for such cases.

Methods: We compiled cases of traumatic cardiac rupture and penetrating cardiac injuries accompanied by severe hemorrhagic shock that necessitated emergency surgery.

Origin, spread, and interspecies transmission of a dominant genotype of BJ/94 lineage H9N2 avian influenza viruses with increased threat.

The H9N2 subtype of avian influenza viruses (AIVs) is widely prevalent in poultry and wild birds globally, with occasional transmission to humans. In comparison to other H9N2 lineages, the BJ/94 lineage has raised more public health concerns; however, its evolutionary dynamics and transmission patterns remain poorly understood. In this study, we demonstrate that over three decades (1994-2023), BJ/94 lineage has undergone substantial expansion in its geographical distribution, interspecies transmission, and viral reassortment with other AIV subtypes, increasing associated public health risks.

Exploring the relationships between self-efficacy, self-care, and glycaemic control in primary care diabetes management.

Objectives: Our study aimed to identify the complex interplay between self-efficacy, self-care practice, and glycaemic control among people with type 2 diabetes mellitus (PWDs) to inform the design of more targeted and effective behavioural interventions in primary care.

Methods: A cross-sectional descriptive study was performed with 294 PWDs managed in primary care. The Diabetes Management Self-Efficacy Scale (DMSES) and Summary of Diabetes Self-Care Activities (SDSCA) questionnaire measured patients' self-efficacy and self-care practice.

Application of artificial intelligence in gastrointestinal endoscopy in Vietnam: a narrative review.

The utilization of artificial intelligence (AI) in gastrointestinal (GI) endoscopy has witnessed significant progress and promising results in recent years worldwide. From 2019 to 2023, the European Society of Gastrointestinal Endoscopy has released multiple guidelines/consensus with recommendations on integrating AI for detecting and classifying lesions in practical endoscopy. In Vietnam, since 2019, several preliminary studies have been conducted to develop AI algorithms for GI endoscopy, focusing on lesion detection.

Translation, Cultural Adaptation, and Validation of the Vietnamese Version of the Vocal Fatigue Index.

Objectives: The Vocal Fatigue Index (VFI) is widely used to identify vocal fatigue, but no Vietnamese version exists. This study aimed to translate the VFI into Vietnamese and evaluate its reliability and validity among primary school teachers in Vietnam.

Methods: The VFI was translated using a forward-backward method.

National extension workshop: Connecting learners and educators to broaden reach and maximize impact in the 21st century.

Disseminating training nationwide through the Cooperative Extension Service, particularly as global demand for poultry products continues to increase, is critical for sustainable poultry production. Increasing urbanization, globalization of the poultry industry, consumer interest in animal welfare and labor shortages present opportunities for Extension professionals to engage diverse audiences and increase agricultural literacy. As part of the Poultry Science Association's 2024 National Extension Workshop, we presented examples of initiatives aimed at connecting educators, teachers and learners to address some of the pressing issues facing the poultry industry.