Exploring cryopreservation alternatives for Dirofilaria immitis microfilariae.

Canine Heartworm Disease, caused by Dirofilaria immitis, primarily affects canids and felids. The earliest studies on cryopreservation were carried out at -70°C, achieving acceptable survival rates, however microfilariae (mf) showed alterations both in morphology and motility. Thereafter, liquid nitrogen was used representing an excellent tool for long-term preservation, albeit it is expensive and requires trained personnel.

Cisplatin-functionalized dual-functional bone substitute granules for bone defect treatment after bone tumor resection.

Invasive bone tumors pose a significant healthcare challenge, often requiring systemic chemotherapy and limb salvage surgery. However, these strategies are hampered by severe side effects, complex post-resection bone defects, and high local recurrence rates. To address this, we developed dual-functional bone substitute biomaterials by functionalizing commercially available bone substitute granules (Bio-Oss® and MBCP®+) with the established anticancer agent cisplatin.

Updating the epidemiology of canine leishmaniosis in the United Kingdom through the use of electronic health data.

Dogs infected with Leishmania infantum have been increasingly reported in the UK mostly related to imported/travelled dogs. Up-to-date epidemiologic data are essential for a better control of this zoonotic disease in such emerging areas. This study aimed for the first time, to estimate the percentage and temporal variation of dog and cat samples testing positive for L.

A scoping review of transmission models for soil-transmitted helminth infections to underpin the development of a transmission model for .

Soil-transmitted helminth (STH) infections afflict people worldwide, especially in tropical and subtropical regions. is distinctive from other STH nematodes by its complex life cycle features of autoinfection, parthenogenesis, and environmental reproduction. This scoping review aims to identify the structures, features, and techniques employed in existing STH models, emphasizing their potential application in describing infection dynamics.

Assessing veterinarians' opinions of antimicrobial stewardship initiative acceptability for farm practice in Wales.

Background: Antimicrobial stewardship (AMS) initiatives are imperative to safeguard antimicrobials and reduce resistance. Across the UK, AMS relies in part on the self-regulation of the veterinary profession, particularly in farm animal practice, alongside voluntary schemes and quality assurance systems. In Europe, AMS initiatives are often statutory and require responsibility from several sectors.

Preparation and characterization of aptamer-based sorbent for the selective extraction of zearalenone and its derivatives from human urine.

The aim of this work is the development of a biomimetic strategy involving a molecular recognition mechanism using aptamers immobilized on a solid support for the analysis of the mycotoxin zearalenone (ZEA) and two of its derivatives in human urine: alpha-zearelenol (α-ZEL) and beta-zearelenol (β-ZEL). Three oligonucleotide sequences reported in the literature as being specific to ZEA were thus covalently grafted onto activated sepharose, and a thorough study of the percolation and washing conditions was performed to promote the selective retention of the three targeted compounds. With the optimized extraction procedure, a strong and selective retention was obtained for ZEA and to a lesser extent α-ZEL and β-ZEL, with extraction recoveries of 88±9%, 77±15%, and 45±12% respectively, in standard solutions.

Surface monitoring of L. monocytogenes by real-time fluorescence and colorimetric LAMP.

Listeria monocytogenes is a major foodborne pathogen affecting developing, and developed countries. The analysis of food contact surfaces in food industries is key for better controlling this pathogen. The current study focused on the development, optimization, and evaluation of a rapid and simple method for the detection of L.

Lidocaine's Ineffectiveness in Mitigating Lipopolysaccharide-Induced Pain and Peristaltic Effects in Horses.

The present study involved seven horses in a randomized crossover clinical trial to evaluate the effect of lidocaine on horses with induced endotoxemia. Horses received intravenous lidocaine (1.5 mg/kg bolus, followed by 0.

Extrinsic and intrinsic drivers of prevalence and abundance of hard-bodied ticks (Acari: Ixodidae) in one-humped camel ().

Background: Ticks are ectoparasites and can be vectors of a wide range of pathogens, posing significant health risks to livestock. In the Sahara Desert of Algeria, particularly among one-humped camels (), there is a need to better understand the factors influencing tick infestation patterns to improve livestock management and health outcomes.

Objectives: This study aimed to investigate the prevalence, intensity, and abundance of hard-bodied ticks (Acari: Ixodidae) among dromedaries, examining both intrinsic factors (sex, age, coat color) and extrinsic variables (farming systems, vegetation types, climate zones, and elevation) that might influence tick infestation in this region.

Wings of Knowledge: Navigating Learner Confidence and Cognitive Load in Avian Radiography with a Low Fidelity Model.

In veterinary first opinion practice, radiography is an important diagnostic tool for avian patients. Teaching of such diagnostic skills to learners is usually conducted using teaching models in clinical skills laboratories. The aim of this work is to evaluate the impact of using a teaching model for avian radiography positioning by measuring learner's cognitive load, confidence, satisfaction, and assessing learning by OSCE assessment.

Setting Up and Running Online Communities of Practice (CoPs) for Veterinary Educators.

Communities of practice (CoPs) are social systems consisting of individuals who come together to share knowledge and solve problems around a common interest. For educators, membership of a CoP can facilitate access to expertise and professional development activities and generate new collaborations. This teaching tip focuses on online CoPs and provides tips for setting up and running such communities.

Utilizing Stakeholder Consultations to Identify Context-Specific Professional Skills for Veterinary Graduates in Bangladesh.

Despite professional skills being part of the Day One Competences published by national as well as international accreditation bodies, veterinary schools in Bangladesh have limited associated teaching within their curricula. Therefore, our study aimed to identify the most important professional skills for veterinarians in Bangladesh through local consultation to inform future initiatives to change the curriculum. Eleven focus groups were conducted with 45 stakeholders who included veterinarians who supervise students on workplacements, faculty, recent graduates, final year students, and clients.

A Global Evaluation of Generic Antimicrobial Prescribing Competencies for Use in Veterinary Curricula.

The European Society for Clinical Microbiology and Infectious Diseases (ESCMID) developed consensus-based generic competencies in antimicrobial prescribing and stewardship. These may be useful in structuring and evaluating antimicrobial prescribing education to veterinary students, but their applicability has not been evaluated. We aimed to evaluate whether the ESCMID competencies are currently taught and how relevant they are to veterinary prescribing in veterinary schools globally.

Global landmark: 2023 marks the worst year for dengue cases with millions infected and thousands of deaths reported.

Objectives: In 2023, the world experienced the worst dengue virus (DENV) outbreak on record. The study aimed to identify global regions and continents with high burden of dengue in 2023.

Design: We collected data on the number of DENV cases and deaths reported by various countries to the World Health Organization and World Health Organization regional offices.

Editorial: Protein post-translational modifications in the nervous system: from development to disease and ageing.

PTMs are crucial for biological processes contributing to healthy organ function. Protein post-translational modifications (PTMs), such as phosphorylation (P), acetylation (Ac), SUMOylation (SUMO), S-nitrosylation (Nitro), ubiquitination (Ub) and glycosylation (Glyco), affect a wide range of cellular and biological functions as depicted in this cartoon. Perturbations lead to severe consequences for the normal function of the brain and other organs, such as muscle.