Meconium Influences Pulmonary Short-Chain Fatty Acid Concentration in Porcine Meconium Aspiration Model.

Introduction: The factors influencing meconium aspiration syndrome (MAS) severity remain poorly understood. In a piglet model of MAS, we hypothesized the respiratory microbiome would reflect the bacterial signature of meconium with short-chain fatty acid (SCFA) accumulation as a byproduct of bacterial fermentation.

Methods: Cesarean section at approximately 115-day term was performed on two sows.

Role of gamma irradiation and disaccharide trehalose to induce immune responses in Syrian hamster model against Iranian SARS-CoV-2 virus isolate.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus is the causative agent of the emerging zoonotic respiratory disease. One of the most important prerequisites for combating emerging diseases is the development of vaccines within a short period of time. In this study, antigen-irradiated, inactivated SARS-CoV-2 viruses and the disaccharide trehalose were used to enhance immune responses in the Syrian hamster.

Utilization of mannan oligosaccharides as antibiotic substitutes in laying hens.

Among global concerns about antibiotic resistance, it is necessary to identify food-safe alternatives to enhance production. This study aimed to evaluate the impact of mannan oligosaccharides (MOS) inclusion to replace antibiotic growth promoters (AGP) in the diets of ISA Brown laying hens aged between 23 and 31 weeks. Two hundred forty hens were grouped into five treatments: Control, AGP (130 ppm of enramycin 8.

Effects of melatonin on the mitogen-activated protein kinase signaling genes in hypoxic Leydig cells.

Leydig cells play a crucial role in male reproductive physiology, and their dysfunction is often associated with male infertility. Hypoxia negatively affects the structure and function of Leydig cells. This study aimed to investigate the impact of melatonin on the c-Jun N-terminal kinase (Jnk), P38, and extra-cellular signal-regulated kinases 1 and 2 (Erk1/2) mitogen-activated protein kinase (MAPK) signaling pathways in TM3 mouse Leydig cells under hypoxia induced by cobalt (II) chloride (CoCl).

Occurrence of biofilm forming fungal species and in vitro evaluation of anti-biofilm activity of disinfectants used in drinking water.

Fungal contamination in drinking water has garnered considerable attention over the past few decades, especially considering the detrimental consequences of pathogenic fungal species on both human and animal health. The formation of biofilms by certain species is a considerable factor contributing to the emergence of severe fungal infections. This research was designed to isolate and identify fungi, particularly those capable of forming biofilms from 150 samples of drinking water sourced from various locations.

Protective effects of Mito-TEMPO on ischemia-reperfusion injury in a mouse testicular torsion and detorsion model.

Testicular ischemia-reperfusion (I/R) injury during testicular torsion is strongly influenced by oxidative stress caused by excessive accumulation of unscavenged reactive oxygen species. This study aimed to investigate the effects of intra-peritoneal administration of Mito-TEMPO (MT) on I/R injury in testicular torsion/detorsion (T/D) in mice. Forty-two male mice were divided into seven groups including 1 control and 6 treatment groups (360° T/D, 720° T/D, 360° T/D + 0.

Frequency of in dogs from the municipality of Veracruz, Mexico.

Infectious canine cyclic thrombocytopenia or canine anaplasmosis is an infectious disease caused by . In Mexico, cases of human infection have been reported. The present cross-sectional study aimed to determine the frequency of infection in the municipality of Veracruz, Mexico, by nested polymerase chain reaction method.

Viability of guinea pig () spermatozoa diluted in Tris-buffer extenders stored at 5.00 ˚C.

The cooling procedure markedly diminishes the quality of guinea pig () sperms, primarily because their membranes are highly susceptible to this process. This susceptibility triggers the generation of reactive oxygen species and free radicals, ultimately leading to lipid peroxidation in the sperm membrane. Surprisingly, there has been a lack of research on the use of Tris-based extenders to safeguard guinea pig sperm under refrigeration conditions.

A comparative study of proximate compositions, phytochemical constituents, and anti-nutritional contents of pulps and seeds of fruit.

is a tropical wild edible plant that is locally consumed in the Southern Nations, Nationalities, and Peoples Regional state of Ethiopia. However, there is limited information comparing nutritional, phytochemical, and anti-nutritional factors present in fruit pulps and seeds of the fruit. Therefore, this study aimed to investigate and compare the proximate compositions, phytochemical constituents, and anti-nutritional contents of the fruit pulp and seeds.

Residual antibiotics in milk samples: Assessing the risk and prevalence in Bangladesh.

The study aimed to analyze five commonly used veterinary antibiotics: tetracycline (TC), oxytetracycline (OTC), doxycycline (DOX), chlortetracycline (CTC), and enrofloxacin (ENR) in different types of milk samples, risk estimation, and to investigate the correlation between the presence of multiple antibiotic residues. About 27 milk samples, such as raw milk from collection centers, processed milk from processing plants, pasteurized, UHT, and flavored milk from retail stores, were examined using RP-HPLC against five veterinary antibiotics in Dhaka, Bangladesh. The correlation between antibiotics was analyzed using Pearson's correlation test.

Omega-3 Fatty Acids, Lecithin, Sterols, Vitamins and Lipid Oxidation of Olein and Super Olein Fractions of Fish Oil Produced by Winterization.

To concentrate omega-3 fatty acids (-3) in fish oil (FO), olein and super olein fraction (OF) of FO were produced by winterization. For this purpose, FO was slowly cooled to -50°C (24 h), the mixture of crystallized and non-crystallized phases was separated, filtrate was coded as OF (yield 32%), 35% of OF was kept for storage study and analytical purpose, remaining 65% was further slowly cooled down to -75°C (24 h) and filtered, filtrate was coded as super olein (SF, yield 23%). GC-MS analysis showed that unsaturated fatty acids increased due to successive winterization.

Prevalence and Zoonotic Risk of Multidrug-Resistant in Bovine Subclinical Mastitis Milk: Insights Into the Virulence and Antimicrobial Resistance.

The emergence of antibiotic-resistant microorganisms has made antimicrobial resistance a global issue, and milk is a potential source for the propagation of resistant bacteria causing zoonotic diseases. Subclinical mastitis (SCM) cases, often overlooked and mixed with normal milk in dairy farms, frequently involve , which can spread through contaminated milk. We conducted this study to determine the prevalence of virulence genes, antibiotic resistance genes (ARGs), antimicrobial susceptibility, and the genetic relatedness of multidrug-resistant (MDR) Shiga toxin-producing (STEC) isolated from SCM milk.

Salt Contents in Fermented Dairy Products: A Strategic Blueprint for Healthier Intake.

This study aimed to estimate the quantity and trends of salt intake from industrial fermented dairy products, develop strategies to reduce salt content, and inform policymakers on promoting public health through healthier dairy options. A cross-sectional study was conducted on fermented dairy products. Seventy-nine random samples were selected, and the salt (NaCl %) content was determined by potentiometric titration after sample preparation and homogenization; also samples were analyzed for their moisture (oven drying method).

Prevalence, antimicrobial susceptibility profiles and resistant gene identification of bovine subclinical mastitis pathogens in Bangladesh.

Subclinical mastitis (SCM), a silent threat in the dairy sector of Bangladesh poses a significant economic impact and serves as a potential source of infection for healthy cows, hindering efforts to achieve milk self-sufficiency. Despite the importance of this issue, limited research has been conducted on mastitis in Sylhet region of Bangladesh. This study aimed to investigate the molecular prevalence, antimicrobial susceptibility profile and resistant genes detection on pathogens ( and causing SCM.

Examining pharmacoepidemiology of antibiotic use and resistance in first-line antibiotics: a self-controlled case series study of in small companion animals.

Background: Clinicians need to prescribe antibiotics in a way that adequately treats infections, while simultaneously limiting the development of antibiotic resistance (ABR). Although there are abundant guidelines on how to best treat infections, there is less understanding of how treatment durations and antibiotic types influence the development of ABR. This study adopts a self-controlled case study (SCCS) method to relate antibiotic exposure time to subsequent changes in resistance patterns.

as a sentinel in the assessment of antimicrobial resistance in the tilapia production chain: from production environment to the final product.

Introduction: The intensification of tilapia production has increased animal density in tanks, leading to more frequent exposure to pathogenic agents and compromising the quality of fish products. Antimicrobial resistance is a global concern that affects human treatment, and sentinel microorganisms like are crucial for monitoring production chains, especially in aquaculture, where research is still limited. The aim of this study was to identify the presence of and investigate its antimicrobial resistance profiles throughout the entire tilapia production chain.

Ticks (Acari: Ixodidae) and tick-borne diseases in Cameroon: Current understanding and future directions for more comprehensive surveillance.

Despite the high burden of human and animal infectious diseases in Cameroon, implementing integrative approaches to managing and controlling arthropods and their pathogens remains challenging. Surveillance should be designed to detect diseases and provide relevant field-based data for developing and implementing effective control measures to prevent outbreaks before significant public and animal health consequences can occur. Nowadays, ticks are considered the primary vectors of animal diseases in the world, and the second vector of human diseases after mosquitoes.

Clinical and laboratory findings of hookworms ( spp.) in naturally infected adult dogs.

Intestinal parasites of the genus are the most prevalent in coproparasitological examinations and necropsies of dogs in Brazil. Although adult dogs often remain asymptomatic when infected, there is limited published information concerning the laboratory and clinical findings and severity of infection in symptomatic adult dogs. Therefore, this study aimed to characterize the clinical and laboratory findings of adult -infected dogs.

Blood cortisol and faecal cortisol metabolite concentrations following an ACTH challenge in unanaesthetized brown bears ().

Faecal cortisol metabolites (FCMs) are increasingly used to index physiological stress in wildlife. Cortisol and other stress hormones act to mobilize glucose, providing energy for the organism to respond to environmental perturbations. Cortisol, the predominant glucocorticoid (GC) in most mammals, is metabolized by the liver and excreted as FCMs.

Multi-omics characterization of improved cognitive functions in Parkinson's disease patients after the combined metabolic activator treatment: a randomized, double-blinded, placebo-controlled phase II trial.

Parkinson's disease is primarily marked by mitochondrial dysfunction and metabolic abnormalities. We recently reported that the combined metabolic activators improved the immunohistochemical parameters and behavioural functions in Parkinson's disease and Alzheimer's disease animal models and the cognitive functions in Alzheimer's disease patients. These metabolic activators serve as the precursors of nicotinamide adenine dinucleotide and glutathione, and they can be used to activate mitochondrial metabolism and eventually treat mitochondrial dysfunction.

Potential of soybean-velvet bean combination tempe in improving cognitive function.

Velvet bean is a native Indonesian legume containing L-dopa, yet it remains underutilized. The aim of this study was to analyze the effects of different types of tempe (soybean, velvet bean, and their combination) on cognitive function, brain histology, dopamine levels, and serum β-amyloid in rats, as well as to identify the parameters most influencing cognitive function, including brain mass and volume, hippocampal neuron count, and dopamine and β-amyloid levels. An experimental study was conducted using a completely randomized design with one factor: the protein source of diet.

Exploring the anti-inflammatory and antiapoptotic properties of phloroglucinol on pancreatic cells in diabetic models: In silico and in vivo study.

Pancreatic cell damage in diabetes mellitus is closely linked to inflammation and apoptosis. This study aimed to investigate the protective effects of phloroglucinol on pancreatic cells in a streptozotocin-induced diabetic model by assessing its anti- inflammatory and anti-apoptotic mechanisms. Phloroglucinol ligand and the structures of Bax, Bcl-2, and caspase-3 proteins were sourced from the PubChem database.

Does gene polymorphism affect the duration of orogastric tube use in moderate to late preterm neonates? A cross-sectional study in Indonesia.

Premature and low birth weight neonates often struggle with oral intake due to immaturity or respiratory distress. Forkhead box protein 2 gene () is predicted to influence oral feeding ability in newborns, but studies assessing the role of this gene in influencing oral feeding ability are limited. The aim of this study was to investigate the role of gene polymorphism, particularly single nucleotide polymorphism (SNP) rs17137124, on the duration of orogastric tube (OGT) use in moderate to late preterm neonates.

Potential of ant nest extract () for protection of testicular morphometry, epididymal functions, and sperm quality in male rats with alloxan- induced diabetes.

Medicinal herbs, such as the ant nest plant (), are promising for the management of diabetes mellitus-associated infertility. The aim of this study was to evaluate the biological activity of the ant nest plant and its capacity to mitigate the adverse effects of alloxan-induced diabetes on testicular morphology, epididymal function, and sperm quality in male rats. The tuber of the ant nest plant was extracted using methanol and then subjected to phytochemical screenings.