Evaluation of the Accuracy and Performance of Two Commercial Pregnancy-Associated Glycoprotein Tests for Early Pregnancy Detection in Cows.

The aim of this study was to determine and compare the diagnostic accuracies of two commercial pregnancy-associated glycoprotein tests, Alertys OnFarm Pregnancy Test (AOPT) and Alertys Milk Pregnancy Test (AMPT), for early pregnancy diagnosis in dairy cattle. Holstein cows (n = 124) were used in the study. Whole blood samples were collected from the jugular vein 28 days after fixed-time artificial insemination (FTAI).

IFN-β production induced by PRRSV is affected by GP3 quantity control and CLND4 interaction.

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most harmful pathogens in the swine industry. Our previous studies demonstrated that the small extracellular domain (ECL2) of CLDN4 effectively blocks PRRSV infection. In this study, we explored the in vivo administration of swine ECL2 (sECL2) and found that it blocked HP-PRRSV infection and alleviated histopathological changes in organs.

The Role of Nutrition in Managing Muscle Disorders.

Many myopathies in horses can be managed by exercise regimes and dietary modifications. This includes modifying the amount of nonstructural carbohydrate, fat, amino acids, vitamin E, and selenium based on the horse's specific myopathy, metabolic status, exercise program, and optimal body weight. Because dietary recommendations differ substantially between myopathies, it is imperative to establish a specific diagnosis.

Zoonotic diseases awareness and food safety practices among livestock farmers in Nepal.

Interactions between humans and livestock could increase the risk of zoonotic disease transmission. In addition, limited knowledge of zoonoses and foodborne diseases among livestock farmers could heighten the risks of foodborne illness and outbreaks of zoonotic diseases. This study evaluated the awareness of zoonotic diseases and preventive practices for zoonotic and foodborne diseases among livestock farmers of the Chitwan, Rupandehi, and Tanahun districts of Nepal by conducting a cross-sectional survey of 280 livestock farmers.

Performance of 1-year-old camel calves fed a basal ration of alfalfa hay supplemented with different levels of concentrate.

Background: The use of a high-concentrate diet in fattening camels may have significant effects on growth performance and digestion as well as economic returns. This experiment was designed to study the effects of feeding different levels of concentrate in their diet on growth performance and digestion in a desert climate.

Methods: Eighteen 12-month-old male camel calves were used, and divided into three treatments of six each.

A review of sustainable cattle genetic improvement in the Peruvian Highlands.

Cattle breeding in the highlands of Peru is an important economic activity at the level of the entire rural extension, because it serves as an economic reserve for rural families and forms an integral part of the agricultural producer's culture. This review aimed to provide a literature- and research-based approach to the fundamental aspects of a national genetic improvement plan, emphasising the efficacy of using a bovine germplasm of high genetic quality as an initiative to implement genetic improvement programmes. The concepts to be implemented in national livestock farming include high yield, feed conversion efficiency, and minimum greenhouse gas emissions.

The biological function of and its application in animal husbandry: a review.

, is a herbaceous plant of the Asteraceae family which is a traditional Chinese herbal medicine. It is often used for dehumidification, antiemetics, spleen strengthening and antipyretic effects. is rich in various bio-active substances and has many biological functions, for instance anti-inflammatory, antioxidant and antiviral effects.

Development of a SYBR Green-based RT-qPCR assay for the rapid detection of peste-des-petits-ruminants virus.

Peste-des-petits-ruminants (PPR) is primarily a disease of small ruminants caused by peste-des-petits-ruminants virus (PPRV; , ), formerly the small ruminant morbillivirus. PPRV can cause significant morbidity and mortality in small ruminants and a significant economic impact. Conventional reverse-transcription PCR (RT-PCR), and probe-based and SYBR Green-based RT quantitative real-time PCR (RT-qPCR), are employed for the molecular detection of PPRV.

Development of a qPCR assay to identify and differentiate insect-associated strains of the complex.

The complex contains important opportunistic pathogens of humans and vertebrate animals, as well as insects and other invertebrates. To date, the methods used for the identification of species within the genus , including PCR assays, have poor discriminatory power and may require further molecular typing or genomic sequence analysis to determine clinical relevance. We developed a duplex TaqMan probe-based quantitative real-time PCR (qPCR) assay targeting the gene, which is involved in chitin degradation and transport, and the gene, which is involved in urease production.

Geographical variation in antimicrobial resistant Salmonella Schwarzengrund from chicken meat in Japan.

Chicken meat is a major source of foodborne salmonellosis. In Japan, fluoroquinolones and third-generation cephalosporins are the first- and second-choice treatments for Salmonella gastroenteritis, respectively. We investigated the prevalence and antimicrobial resistance of Salmonella in 154 chicken meat products from Hokkaido (42), Tohoku (45), Kanto (5), and Kyushu (62), Japan.

Assessment of Antimicrobial Use for Companion Animals in South Korea: Developing Defined Daily Doses and Investigating Veterinarians' Perception of AMR.

There are global concerns regarding the transmission of antimicrobial-resistant pathogens from animals to humans. Especially, companion animals are increasingly recognized as a potential source due to their close interactions with people, despite a limited number of reported cases. Although, social demands regarding comprehensive surveillance for antimicrobial resistance (AMR) among companion animals are highlighted, there is a lack of a relevant system in South Korea.

Retrospective Study of Chronic Coughing in Dogs in a Referral Centre in the UK: 329 Cases (2012-2021).

Chronic coughing is a common complaint in small animal medicine and it has an extensive differential diagnosis with very different treatment and prognosis. Coughing is considered chronic when it lasts at least 8 weeks. This retrospective study aimed to describe the most common causes of chronic coughing in a population of dogs that presented to a referral hospital in England between January 2012 and December 2021.

Binding and cleavage of pro-urokinase by a tegument extract of Fasciola hepatica newly excysted juveniles activate the host fibrinolytic system.

Plasmin, the final product of fibrinolysis, is a broad-spectrum serine protease that degrades extracellular matrix (ECM) components, a function exploited by multiple pathogens for dissemination purposes. The trematode Fasciola hepatica is the leading cause of fasciolosis, a major disease of livestock and an emerging zoonosis in humans. Infection success depends on the ability of F.

Risk factors and economic losses associated with cystic echinococcosis among livestock in selected pastoral and agro-pastoral areas of Uganda.

Background: Cystic echinococcosis is a parasitic cyclo-zoonotic disease caused by the taeniid Echinococcus and causes significant economic losses in livestock production, yet its impact is often overlooked and under estimated.

Methods: This study investigated the risk factors and economic impact of cystic echinococcosis in livestock, in Uganda's pastoral and agro-pastoral communities. In the study, 14,937 livestock were examined at selected slaughterhouses in Moroto, Kumi, Luwero and Nakasongola districts from March 2019 to February 2020.

Indirect contacts between Danish pig farms - what are the frequencies and risk-reducing measures, and how can they be used in simulation models?

Background: Information on indirect contacts (e.g. contact with visitors and non-porcine species on farms, shared staff and equipment, contact with trucks) is often poorly recorded even though it constitutes a risk in terms of disease transmission.

Occurrence of Leishmania spp. in phlebotomine sand flies and dogs in Guelma region, North-eastern Algeria.

Leishmania spp. are sand fly-borne parasitic protozoa of worldwide distribution that may severely affect the health and welfare of dogs as well as of other mammalian species, including humans. Algeria is among the most affected countries, counting several cases of Leishmania infantum infection in humans and dogs.

Prevalence and molecular characterization of Ascaridia galli in chickens from Minoufiya Governorate, Egypt.

Ascaridia galli causes weight loss, emaciation, anemia, decreased egg production, and sometimes, intestinal obstruction and death in birds, leading to economic losses in the poultry industry. This research aimed to record the occurrence of A. galli in free-range chickens at farmers' houses in three villages in Ashmoun City, Minoufiya Governorate, Egypt.

Occurrence and molecular characterization of Cryptosporidium oocysts in chickens from Egypt, and a meta-analysis for Cryptosporidium infections in chickens worldwide.

Cryptosporidium is a leading cause of diarrhea in children and immunocompromised patients. Various animals and birds can also be infected with this protist, and Cryptosporidium zoonosis is common. A few reports have been published worldwide on Cryptosporidium infections in chickens.

Research and characterization of Cryptosporidium spp. in the domestic cat (Felis catus): First description of C. felis in the state of Goiás and C. muris in the Midwest region of Brazil.

With the increasingly close coexistence between pets and humans, coupled with reports of Cryptosporidium cross-infection between felines and their guardians, research aimed at understanding the epidemiology of these parasites in specific regions has become necessary. The principal objective of this study was to undertake a molecular characterization of Cryptosporidium in fecal samples from felines in the state of Goiás, Brazil (which is unprecedented for the region), and to assess the risk factors involved in the infection of these animals in the aforementioned locale. A total of 95 fecal samples were collected from domestic felines in the municipality of Goiânia, Goiás, Brazil.

Classification of chicken Eimeria species through deep transfer learning models: A comparative study on model efficacy.

Eimeria is a protozoan parasite that causes coccidiosis in various animal species, especially in chickens, resulting in infections characterized by intestinal damage, hemorrhagic diarrhea, lethargy, and high mortality rates in the absence of effective control measures. The rapid spread of these parasites through ingestion of food and drinking water can seriously endanger animal health and productivity, leading to significant economic losses in the chicken industry. Chicken Eimeria species are difficult to identify by conventional microscopy due to similarities in oocyst morphologies.

Rapid and Environment-Friendly LC-MS/MS for Simultaneous Analysis of Amino Acids in Veterinary Medicine.

Background: Amino acid supplements are crucial for animal health and productivity. Traditional analysis methods face limitations like complexity, long testing times and toxic reagents. Therefore, a more efficient and reliable method is needed.

Effects of Production System With or Without Growth-Promoting Technologies on Growth and Blood Expression of (Cyto)Chemokines and Heat Shock and Tight Junction Proteins in and Breeds During Summer Season.

Heat stress (HS) induced by global warming is a real welfare, productivity, and economic burden of cattle production. However, some cattle breeds have superior physiological adaptive traits to others, yet the underlying molecular mechanisms are not fully defined. The present study aimed, therefore, to determine the expression profile of stress-related molecular signatures in the blood of thermosensitive Angus () and thermotolerant Brahman () cattle breeds managed without (CON) or with growth-promoting technology (TRT) during the summer (April-October, 2023) season in Colorado, US.

Biological Characteristics and Whole-Genome Analysis of a Porcine Phage.

(1) Background: In recent years, the increasing emergence of multidrug-resistant pathogens in pig farms has begun to pose a severe threat to animal welfare and, by extension, public health. In this study, we aimed to explore the biological characteristics and genomic features of bacteriophages that are capable of lysing porcine multidrug-resistant , which was isolated from sewage. In doing so, we provided a reference for phage therapies that can be used to treat multidrug-resistant strains.

Equine Herpesvirus-1 Induced Respiratory Disease in Dezhou Donkey Foals: Case Study from China, 2024.

Equine herpesvirus-1 (EHV-1) is a significant pathogen that causes substantial economic losses in the equine industry worldwide, which leads to severe respiratory diseases and abortions in horses. However, reports of EHV-1 infection in donkeys are limited, particularly in China. This case study reported an EHV-1-induced respiratory disease in Dezhou donkey foals in Shandong Province, China, in July 2024.