Selection of alkaliphilic Bacillus pectate lyases based on reactivity and pH-dependent stability in simulated environment for industrial applications.

Pectate lyases, known for their alkaliphilic nature, are ideal for industrial applications that require specific pH conditions, particularly in industries such as textiles and pulp extraction. These enzymes, primarily from the polysaccharide lyase family 1 (PL1) of different microbial sources, play a vital role in polysaccharide degradation. Given the potent pectinolytic activity of Bacillus pectate lyases, targeting these enzymes is crucial for identifying the most effective candidates.

Investigation of the impact of R273H and R273C mutations on the DNA binding domain of P53 protein through molecular dynamic simulation.

The P53 protein, a cancer-associated transcriptional factor and tumor suppressor, houses a Zn ion in its DNA-binding domain (DBD), essential for sequence-specific DNA binding. However, common mutations at position 273, specifically from Arginine to Histidine and Cysteine, lead to a loss of function as a tumor suppressor, also called DNA contact mutations. The mutant (MT) P53 structure cannot stabilize DNA due to inadequate interaction.

Nanomaterials impact in phytohormone signaling networks of plants - A critical review.

Nanotechnology offers a transformative approach to augment plant growth and crop productivity under abiotic and biotic stress conditions. Nanomaterials interact with key phytohormones, triggering the synthesis of stress-associated metabolites, activating antioxidant defense mechanisms, and modulating gene expression networks that regulate diverse physiological, biochemical, and molecular processes within plant systems. This review critically examines the impact of nanoparticles on both conventional and genetically modified crops, focusing on their role in nutrient delivery systems and the modulation of plant cellular machinery.

A cross-linked coating loaded with antimicrobial peptides for corrosion control, early antibacterial, and sequential osteogenic promotion on a magnesium alloy as orthopedic implants.

Magnesium (Mg)-based alloys have been recognized as desirable biodegradable materials for orthopedic implants. However, their clinical application has been limited by rapid degradation rates, insufficient antibacterial and osteogenic-promotion properties. Herein, a MgF priming layer was first constructed on AZ31 surface.

developed NiCoO-TiCT nanohybrid towards non-enzymatic electrochemical detection of glucose and hydrogen peroxide.

Owing to the adverse consequences of excess glucose (Glu) and hydrogen peroxide (HO) on humans, it is imperative to develop an electrochemical sensor for detection of these analytes with good selectivity and sensitivity. Herein, a nanohybrid comprising nickel cobaltite nanoparticles (NiCoO NPs) embedded on conductive TiCT nanosheets (NSs) has been prudently designed and employed for the electrochemical detection of Glu and HO. The developed nanohybrid has been systematically characterized using morphological and spectral techniques, and then immobilized on a glassy carbon electrode (GCE).

Biogenesis and functional implications of extracellular vesicles in cancer metastasis.

Extracellular vesicles (EVs) play a crucial role in the complex process of cancer metastasis by facilitating cellular communication and influencing the microenvironment to promote the spread and establishment of cancer cells in distant locations. This paper explores the process of EV biogenesis, explaining their various sources that range from endosomal compartments to plasma membrane shedding. It also discusses the complex mechanisms that control the sorting of cargo within EVs, determining their chemical makeup.

Decoding poly (RC)-binding protein 1 (PCBP1), the underrated guard at the foothill of ferroptosis.

PCBP1 is a multifunctional adaptor protein, whose function as an iron chaperone and epigenetic regulator of several chemical messengers involved in ferroptosis has garnered much attention. Herein, this review, several attempts have been made to simplify our understanding of the complex roles of PCBP1. The review begins by elucidating the relevance of PCBP1 in key events governing ferroptosis.

Targeting IKZF1 via HDAC1: Combating Acute Myeloid Leukemia.

Acute myeloid leukemia (AML) accounts for 1.3% of all cancers, with a limited survival of only 30%, and treating AML is a continuous challenge in medicine. IKZF1 is a DNA-binding protein that is highly mutated and undruggable but significant in causing AML.

Role of intuition on problem solving style-Mediating effect of mindfulness.

The present study investigates the mediating effect of mindfulness on the role of intuition upon problem solving style among university students in India. 755 university and college students in India (Male = 310, Female = 445) completed a questionnaire that contained items of types of intuition scale (TintS) developed by Pretz et.al.

Mechanism to disrupt ESCRT-mediated intracellular trafficking through Vps28-small molecules interaction: an in silico approach.

The ESCRT (Endosomal Sorting Complex Required for Transport) machinery comprising protein complexes ESCRT-0 to ESCRT-III and Vps4 plays a pivotal role in intracellular trafficking, a process of endocytosing cell surface proteins into the cell for various biological activities. The ESCRT protein complexes are sequentially assembled which interact amongst each other to form a functional ESCRT machinery. Deregulation of these events are shown to be involved in various disease development including tumor formation and viral infections.

Binding of therapeutic Fc-fused factor VIII to the neonatal Fc receptor at neutral pH associates with poor half-life extension.

Fusion of therapeutic proteins to the Fc fragment of human IgG1 promotes their FcRn-mediated recycling and subsequent extension in circulating half-life. However, different Fc-fused proteins, as well as antibodies with different variable domains but identical Fc, may differ in terms of extension in half-life. Here we compared the binding behaviour to FcRn of Fc-fused FVIII, Fc-fused FIX and two human monoclonal HIV-1 broadly-neutralizing IgG1, m66.

Is periodontitis triggering an inflammatory response in the liver, and does this reaction entail oxidative stress?

An inflammatory disorder known as periodontitis impacts 10%-15% of adults. The pathogenesis of periodontal disease and system illnesses such as liver disease is similar in that it is marked by a chronically dysregulated inflammatory reaction. Injuries caused by inflammation and oxidative stress, such as hepatotoxicity caused by drugs, often affect the liver.

Organelle-Specific Smart Supramolecular Materials for Bioimaging and Theranostics Application.

In cellular environments, certain synthetic molecules can form nanostructures via self-assembly, impacting molecular imaging, and biomedical applications. Control over the formation of these self-assembled nanostructures in subcellular organelle is challenging. By the action of stimuli, either present in the cellular environment or applied externally, in situ generation of molecular precursors can lead to accumulation and supramolecular nanostructure formation, resulting in efficient bioimaging.

Transcriptomic, mutational and structural bioinformatics approaches to explore the therapeutic role of FAP in predominant cancer types.

Fibroblast activating protein (FAP) is a cell surface marker of cancer-associated fibroblasts with a distinct pro-tumorigenic role. The present study analyzed the pan-cancer expression; and clinical and mutational profiles of the FAP coding gene. Molecular dynamics simulation (MDS) deciphered the backbone dynamics and energetics of FAP.

Promoting Apoptosis in MCF-7 Cells via ROS Generation by Quinolino-triazoles Derived from One-Pot Telescopic Synthesis.

Inhibition of vascular endothelial growth factor receptor 2 (VEGFR-2) facilitates potent antiangiogenic and anticancer responses. In this regard, the development of effective pharmacophores, i.e.

Comparative efficacy of extract delivery PEG--PCL, niosome, and their combination against genotype T4: characterization, inhibition, anti-adhesion, and cytotoxic activity.

Background: spp. is a waterborne, opportunistic protozoan that can cause amebic keratitis and granulomatous amebic encephalitis. is a native tree in Malaysia, and its extracts possess a broad range of biological activities.

Sialic Acid-Targeted Ru(II)/Ir(III)/Re(I) Complexes for Ferroptosis Induction in Triple-Negative Breast Cancer.

Ferroptosis has been recognized as an iron-based nonapoptotic-regulated cell death process. In the quest of resisting the unyielding vehemence of triple-negative breast cancer (TNBC), herein we have showcased the ferroptosis-inducing heteroleptic [], [], and [] complexes, enabling them to selectively target "sialic acid", an overexpressed cancer cell-surface marker. The open-circuit potential (OCP) measurements in live cancer cells revealed the specific interaction between TNBC and the complexes, whereas control experiments with normal cells did not exhibit such interactions.

Biochemical Signal-Induced Supramolecular Hydrogelation for Structured Free-Standing Soft Material Formation.

Cells coordinate their activity and regulate biological processes in response to chemical signals. Mimicking natural processes, control over the formation of artificial supramolecular materials is of high interest for their application in biology and medicine. Supramolecular material that can form in response to chemical signals is important for the development of autonomously responsive materials.

Occurrence of protease-like catalytic activity in the polyclonal IgG in schizophrenia and bipolar disorder.

Neuro-immune dysfunction and inflammation are said to be involved in the aetiology of major mental illnesses. This study describes the assessment of the protease-like catalytic function of serum IgG in psychiatric disorders, schizophrenia and bipolar disorder, along with healthy controls of Indian ethnicity. Systemic lupus erythematosus patients experiencing neuropsychiatry conditions were included as comparators for the comprehensive evaluation of IgG catalytic function.

Visible light responsive heterophase Titania monoliths for the fast and efficient photocatalytic decontamination of organic pollutants.

The article reports the synthesis of an ordered mesoporous network of heterophase TiO monoliths as a visible light-responsive photocatalyst using tri-block copolymers of Pluronic F108, P123 and F127 as structure-directing agents (SDAs) and temperature-controlled calcination (450-650 °C) has been carried out by direct templating-assisted hydrothermal approach. The structural/surface morphology and topographical properties of the photocatalyst are characterized using FE-SEM-EDAX, HR-TEM-SAED, p-XRD, VB-XPS, PLS, TG/DTA, UV-Vis-DRS, BET/BJH and zeta potential analysis. The undoped heterophase mesoporous TiO monoliths with in-built lattice/surface defects exhibit visible light photocatalytic properties, successfully dissipating Reactive Brown 10 (RB-10) dye.

Bioinformatic meta-analysis of transcriptomics of developing Drosophila muscles identifies temporal regulatory transcription factors including a Notch effector.

The intricate mechanism of gene regulation coordinates the precise control of when, where, and to what extent genes are activated or repressed, directing the complex processes that govern cellular functions and development. Dysregulation of gene expression can lead to diseases such as autoimmune disorders, cancer, and neurodegeneration. Transcriptional regulation, especially involving transcription factors (TFs), plays a major role in controlling gene expression.

Optimizing normal tissue objectives (NTO) in eclipse treatment planning system (TPS) for stereotactic treatment of multiple brain metastases using non-coplanar RapidArc and comparison with HyperArc techniques.

Purpose: To optimize NTO parameters in non-coplanar RapidArc (RA) stereotactic radiosurgery (SRS) for multiple brain metastases and compare them with HyperArc (HA) plans.

Materials And Methods: Thirty patients with multiple brain metastases, receiving 21 Gy prescriptions, were retrospectively enrolled, with lesions ranging from two to eight and volumes between 0.27 and 10.

A Comprehensive Review of Antimicrobial Agents Against Clinically Important Bacterial Pathogens: Prospects for Phytochemicals.

Antimicrobial resistance (AMR) hinders the effective treatment of a range of bacterial infections, posing a serious threat to public health globally, as it challenges the currently available antimicrobial drugs. Among the various modes of antimicrobial action, antimicrobial agents that act on membranes have the most promising efficacy. However, there are no consolidated reports on the shortcomings of these drugs, existing challenges, or the potential applications of phytochemicals that act on membranes.