LKB1 and STRADα Promote Epithelial Ovarian Cancer Spheroid Cell Invasion.

Late-stage epithelial ovarian cancer (EOC) involves the widespread dissemination of malignant disease throughout the peritoneal cavity, often accompanied by ascites. EOC metastasis relies on the formation of multicellular aggregates, called spheroids. Given that Liver Kinase B1 (LKB1) is required for EOC spheroid viability and LKB1 loss in EOC cells decreases tumor burden in mice, we investigated whether the LKB1 complex controls the invasive properties of human EOC spheroids.

Cortical encoding of phonetic onsets of both attended and ignored speech in hearing impaired individuals.

Hearing impairment alters the sound input received by the human auditory system, reducing speech comprehension in noisy multi-talker auditory scenes. Despite such difficulties, neural signals were shown to encode the attended speech envelope more reliably than the envelope of ignored sounds, reflecting the intention of listeners with hearing impairment (HI). This result raises an important question: What speech-processing stage could reflect the difficulty in attentional selection, if not envelope tracking? Here, we use scalp electroencephalography (EEG) to test the hypothesis that the neural encoding of phonological information (i.

Simple and easy functionalization of -hydroxy POCOP-M (M = Ni(II), Pd(II)) pincer complexes: synthesis of multimetallic species.

A facile and efficient method for synthesizing mono- and bi-metallic pincer complexes (3-Ni/Ni and 3-Ni/Pd) has been developed. The procedure involves a 1 : 1 stoichiometric reaction of a -hydroxy POCOP pincer complex with cyanuric chloride in the presence of [NaB(OMe)], which selectively affords the mono-substituted pincer complexes (2-Ni and 2-Pd). These complexes are then further reacted with another equivalent of the -hydroxy POCOP pincer complex to produce the desired multimetallic species (3-Ni/Ni and 3-Ni/Pd).

Photochemical carboborylation and three-component difunctionalization of α,β-unsaturated ketones with boronic acids tosylhydrazones.

The reactions of cyclic α,β-unsaturated -tosylhydrazones and alkylboronic acids promoted by 370-390 nm light in the presence of a base give rise to allylic boronic acids that can be trapped as the corresponding pinacolboronates by treatment with pinacol. This reaction features wide scope regarding both coupling partners and functional group tolerance, allowing for the incorporation of a variety of natural product-derived fragments. The allylic boronic acids can be also reacted in a one-pot process with aldehydes, to produce homoallylic alcohols with very high diastereoselectivity.

A novel in-silico model explores LanM homologs among Hyphomicrobium spp.

Investigating microorganisms in metal-enriched environments holds the potential to revolutionize the sustainable recovery of critical metals such as lanthanides (Ln). We observe Hyphomicrobium spp. as part of a Fe/Mn-oxidizing consortia native to the ferruginous bottom waters of a Ln-enriched lake in Czechia.

Development of methodology to support molecular endotype discovery from synovial fluid of individuals with knee osteoarthritis: The STEpUP OA consortium.

Objectives: To develop a protocol for largescale analysis of synovial fluid proteins, for the identification of biological networks associated with subtypes of osteoarthritis.

Methods: Synovial Fluid To detect molecular Endotypes by Unbiased Proteomics in Osteoarthritis (STEpUP OA) is an international consortium utilising clinical data (capturing pain, radiographic severity and demographic features) and knee synovial fluid from 17 participating cohorts. 1746 samples from 1650 individuals comprising OA, joint injury, healthy and inflammatory arthritis controls, divided into discovery (n = 1045) and replication (n = 701) datasets, were analysed by SomaScan Discovery Plex V4.

Daily activity patterns in agoutis ( spp) in response to relaxed predation.

Animals' fitness is determined in a large proportion by the balance in energetic requirements maintained during daily activities, in response to environmental factors. Predation is a major environmental factor influencing the activity patterns of prey, and the deployment of adaptive responses to predation represents a significant cost to prey populations and communities. Experimental removal of predators to study the effect on activity patterns of prey is impractical for vertebrate species.

Hyperspectral imaging in neurosurgery: a review of systems, computational methods, and clinical applications.

Significance: Accurate identification between pathologic (e.g., tumors) and healthy brain tissue is a critical need in neurosurgery.

The STEM leaky pipeline at labor market entry in Spain: The role of job competition and social origin.

The underrepresentation of women in STEM (Science, Technology, Engineering, and Mathematics) majors is well documented. Using high-quality Spanish data, this study examines whether female STEM graduates are less likely to pursue STEM careers than their male counterparts and considers the moderating role of labor market conditions and social origin. We find a pronounced gender effect in initial and subsequent job placement (4-5 years after graduation).

Unintended Consequences of Data Sharing Under the Meaningful Use Program.

Interoperability has been designed to improve the quality and efficiency of health care. It allows the Centers for Medicare and Medicaid Services to collect data on quality measures as a part of the Meaningful Use program. Covered providers who fail to provide data have lower rates of reimbursement.

Protective Effect of High Adherence to Mediterranean Diet on the Risk of Incident Type-2 Diabetes in Subjects with MAFLD: The Di@bet.es Study.

: Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) increases the risk of Type-2 Diabetes (T2DM). The Mediterranean diet (MD) has shown advantages in the management of MAFLD and preventing co-morbidities; however, its relationship with T2DM development in MAFLD has been less investigated. We aimed to evaluate the association of MD adherence with the risk of incident T2DM in the Spanish adult population with MAFLD and according to their weight gain at 7.

Enhancement of the Elastocaloric Performance of Natural Rubber by Forced Air Convection.

We study the enhancement of the elastocaloric effect in natural rubber by using forced air convection to favour heat extraction during the elongation stage of a stretching-unstretching cycle. Elastocaloric performance is quantified by means of the adiabatic undercooling that occurs after fast removal of the stress, measured by infrared thermography. To ensure accuracy, spatial averaging on thermal maps of the sample surface is performed since undercooled samples display heterogeneities caused by various factors.

H-NMR metabolomics identifies three distinct metabolic profiles differentially associated with cardiometabolic risk in patients with obesity in the Di@bet.es cohort.

Background: Obesity is a complex, diverse and multifactorial disease that has become a major public health concern in the last decades. The current classification systems relies on anthropometric measurements, such as BMI, that are unable to capture the physiopathological diversity of this disease. The aim of this study was to redefine the classification of obesity based on the different H-NMR metabolomics profiles found in individuals with obesity to better assess the risk of future development of cardiometabolic disease.

Growth differentiation factor 15 (GDF15) predicts relapse free and overall survival in unresected locally advanced non-small cell lung cancer treated with chemoradiotherapy.

Introduction: Growth differentiation factor 15 (GDF15) is a cytokine of the TGFβ family. Here, we analyzed GDF15 levels in patients with locally advanced non-small cell lung cancer (LA-NSCLC) who participated in OCOG-ALMERA (NCT02115464), a phase II randomized clinical trial, that investigated metformin in combination with standard of care concurrent chemoradiotherapy (cCRT). OCOG-ALMERA was not able to demonstrate benefit in the metformin arm.

Genomic insights into key mechanisms for carbon, nitrogen, and phosphate assimilation by the acidophilic, halotolerant genus Acidihalobacter members.

In-depth comparative genomic analysis was conducted to predict carbon, nitrogen, and phosphate assimilation pathways in the halotolerant, acidophilic genus Acidihalobacter. The study primarily aimed to understand how the metabolic capabilities of each species can determine their roles and effects on the microbial ecology of their unique saline and acidic environments, as well as in their potential application to saline water bioleaching systems. All four genomes encoded the genes for the complete tricarboxylic acid cycle, including 2-oxoglutarate dehydrogenase, a key enzyme absent in obligate chemolithotrophic acidophiles.

Proprioception: An evidence-based review.

Background: Proprioception is an essential sensory function of the body. Proprioception is defined as one's awareness of their body's position and movement through space. It contributes to both the conscious and unconscious awareness of limb and trunk position and movement.

Safety and efficacy of personalised versus standard dosing of linezolid in patients with sepsis (SePkLin): a pragmatic, multicentre, randomised, controlled and superiority clinical trial protocol.

Introduction: Linezolid is a broadly used antibiotic to treat complicated infections caused by gram-positive bacteria. Therapeutic drug monitoring of linezolid concentrations is recommended to maximise its efficacy and safety, mainly haematological toxicity. Different pharmacokinetic/pharmacodynamic targets have been proposed to improve linezolid exposure: the ratio of the area under the concentration-time curve during a 24-hour period to minimum inhibitory concentration (MIC) between 80 and 120; percentage of time that the drug concentration remains above the MIC during a dosing interval greater than 85% and the trough concentration between 2 and 7 mg/L.

Stress timing, trauma exposure, and family resilience differentially affect internalizing and externalizing symptoms at 3, 5, and 7 years of age.

Mental health disorders are associated with decreased quality of life, economic productivity loss, and increased mortality. The association between stressful experiences and psychopathology is well documented. However, studies are needed to understand the impact of timing of stressful events, types of traumatic experiences, and of family resilience on internalizing and externalizing symptoms in early childhood.

Biopolymers as Sustainable and Active Packaging Materials: Fundamentals and Mechanisms of Antifungal Activities.

Developing bio-based and biodegradable materials has become important to meet current market demands, government regulations, and environmental concerns. The packaging industry, particularly for food and beverages, is known to be the world's largest consumer of plastics. Therefore, the demand for sustainable alternatives in this area is needed to meet the industry's requirements.

Lambda carrageenan displays antiviral activity against the infectious pancreatic necrosis virus (IPNV) by inhibiting viral replication and enhancing innate immunity in salmonid cells.

Sulfated polysaccharide lambda carrageenan (λ-CGN) was evaluated for its antiviral effect against IPNV using the in vitro infection model of CHSE-214 salmonid cells. A plaque reduction lysis assay revealed that λ-CGN has an IC of 0.9 μg⋅mL, CC > 128 μg⋅mL and a Selectivity Index (SI) > 142.