Exploiting Cancer Dormancy Signaling Mechanisms in Epithelial Ovarian Cancer Through Spheroid and Organoid Analysis.

Epithelial ovarian cancer (EOC) exhibits a unique mode of metastasis, involving spheroid formation in the peritoneum. Our research on EOC spheroid cell biology has provided valuable insights into the signaling plasticity associated with metastasis. We speculate that EOC cells modify their biology between tumour and spheroid states during cancer dormancy, although the specific mechanisms underlying this transition remain unknown.

Modelling the long-term dynamics and inhibitory effects of crude glycerol impurities in a methanogenic and sulfidogenic UASB bioreactor.

The performance of Upflow Anaerobic Sludge Blanket (UASB) bioreactors treating sulfate (SO) -rich effluents depends on multiple factors, including microbial interactions and operational conditions. The high complexity of these systems necessitates the use of mathematical modelling tools to better understand the process and predict the long-term impacts of various operational variables. In this work, a mathematical model describing the long-term operation of a sulfate-fed 2.

Small-scale genetic differentiation in mean flowering time, but not in plasticity, along a geothermal heating gradient.

Genetic differentiation in traits is assumed to frequently occur in response to divergent natural selection. For example, developmental traits might respond to differences in climate. However, little is known about when and at which spatial scales environmental differences lead to genetic differentiation, and to what extent there is genetic differentiation also in trait plasticity.

Non-infectious pulmonary complications after haematopoietic progenitor transplantation: a diagnostic approach.

Haematopoietic stem cell transplantation (HCT) is an established treatment for a wide variety of haematological diseases, both malignant and non-malignant. Infectious and non-infectious post-HCT pulmonary complications are a major cause of morbidity and mortality, with non-infectious complications becoming more prominent in recent decades as prophylaxis has led to a decrease in infectious complications. Globally, these complications can be divided into three phases (neutropenic, early and late phase) depending on their time of onset in relation to the graft.

Analysis of IL10 gene promoter haplotypes and changes in mRNA expression and soluble levels in patients with basal cell carcinoma.

Interleukin-10 (IL-10) is an immunomodulatory molecule that may play an immunosuppressive role in nonmelanoma skin cancer (NMSC), specifically basal cell carcinoma (BCC). We analyzed the role of IL10 promoter variants in genetic determinants of BCC susceptibility and their association with IL10 mRNA and IL-10 serum levels. Three promoter variants (- 1082 A > G, - 819 T > C, and - 592 A > C) were examined in 250 BCC patients and 250 reference group (RG) individuals.

Quantification of heavy metal exposure in a British population cohort links total mercury levels in plasma with skin tissue-specific changes in mitochondrial-related gene expression.

Heavy metals in our direct environment have profound effects on human health and while some are essential for life, others can be toxic. In vivo studies often focus on clinical features caused by overexposure to, or by deprivation of a heavy metal. However, to understand the cellular impact of heavy metals on health, studies in healthy volunteers before symptom onset are needed.

Intestinal interstitial fluid isolation provides novel insight into the human host-microbiome interface.

Aims: The gastrointestinal (GI) tract is composed of distinct sub-regions, which exhibit segment-specific differences in microbial colonization and (patho)physiological characteristics. Gut microbes can be collectively considered as an active endocrine organ. Microbes produce metabolites, which can be taken up by the host and can actively communicate with the immune cells in the gut lamina propria with consequences for cardiovascular health.

PRMT5 Inhibitor EPZ015666 Decreases the Viability and Encystment of .

Protein arginine methyltransferase 5 (PRMT5) is an enzyme that produces monomethyl arginine (MMA) and symmetric dimethyl arginine (sDMA), post-translational modifications that regulate several cellular processes, including stage conversion in parasitic protozoans. , the etiologic agent of human amebiasis, has two stages in its life cycle, the trophozoite, which is the replicative form, and the cyst, corresponding to the infective phase. The study of the molecular mechanisms that regulate differentiation in this parasite has been overdue because of a lack of efficient protocols for in vitro encystment.

Applying to Integrated Thoracic Surgery Residency: A Survey-Based Cost Analysis.

Background: Application costs are known for other competitive surgical subspecialties but not for integrated thoracic surgery residency (I6). We sought to quantify I6 application costs, assess the value of away rotations and geographic connections, and identify characteristics of matched applicants.

Methods: We retrospectively reviewed the Texas Seeking Transparency in Application to Residency survey-based database for I6 applicants (2019-2022).

Metrics for size-matching in paediatric cardiac transplantation: A narrative review.

Several metrics exist for matching the size of donor to recipient in paediatric cardiac transplantation. Different centres employ different metrics for size-matching to determine the viability of donor hearts. Inconsistent evidence exists, with limited consensus as to the metric for size-matching that is most predictive of outcomes after cardiac transplantation.

Prediction of white matter hyperintensities evolution one-year post-stroke from a single-point brain MRI and stroke lesions information.

Predicting the evolution of white matter hyperintensities (WMH), a common feature in brain magnetic resonance imaging (MRI) scans of older adults (i.e., whether WMH will grow, remain stable, or shrink with time) is important for personalised therapeutic interventions.

Determining rates of genetic testing in the evaluation of autism spectrum disorder and intellectual and developmental disorder.

Genetic testing is recommended by various professional organizations as part of clinical guidelines during the evaluation of autism spectrum disorder (ASD) and other neurodevelopmental disorders. However, previous studies demonstrate that rates of genetic testing are low. This study aimed to identify the rates of genetic testing within a large university healthcare network and factors that may be associated with higher or lower rates.

Caryophyllene Oxide, a Bicyclic Terpenoid Isolated from with Antitumor Activity: In Vivo, In Vitro, and In Silico Studies.

The Annona genus contains some species used in Mexican traditional medicine for the treatment cancer, including . The present study aimed to investigate the anticancer activity of caryophyllene oxide (CO) isolated from using in vivo, in vitro, and in silico approaches. The identification of CO was performed using gas chromatography-mass spectroscopy and NMR methods.

Faecal Metabolome Profiles in Individuals Diagnosed with Hyperplastic Polyps and Conventional Adenomas.

Colorectal cancer (CRC) development is a gradual process in which progressive histological alterations of the intestinal mucosa damage occur over years. This process can be influenced by modifiable external factors such as lifestyle and diet. Most CRC cases (>80%) originate from conventional adenomas through the adenomatous pathway and usually harbour dysplastic cells, whereas the serrated pathway is less frequent (<20% cases) and comprises hyperplastic polyps and other polyps containing dysplastic cells.

Kinesiophobia and Its Correlation with Upper Limb and Hand Functionality Among Individuals with Wrist/Hand Injury: A Cross-Sectional Study.

: Wrist/hand injury incidences in the general population are high and contribute to a significant health problem. Fear of pain from movement can impact physical recovery, contributing to prolonged disability and impaired function in an upper limb after wrist/hand injury. The study's objectives are (1) to evaluate the relationship between kinesiophobia, pain catastrophizing, DASH, and Patient-Rated Wrist Evaluation and (2) to evaluate the data regarding the influence that basal kinesiophobia may have on upper limb functionality after wrist/hand immobilization.

The Influence of Likes and Sexist Attitudes on Adolescent Self-Esteem in Social Networks.

The Internet allows teenagers to express their identity through the publication of images and texts on social networks, but sometimes they may develop self-esteem problems as a result. The present study analyzed self-esteem levels, and their relationship with sexism, Internet use and the influence of likes, in 309 subjects, by asking them about Internet use, social networks, self-esteem and sexism. The results showed low levels of self-esteem, although boys scored higher on the overall scale and for hostile sexism.

Kinesiophobia, catastrophizing, and the duration of immobilization: A prospective study on factors associated with shoulder disability following wrist-hand injuries.

Background: Shoulder pain is a major musculoskeletal problem after wrist-hand immobilization. There is limited evidence regarding the relationship of kinesiophobia or pain catastrophizing with shoulder pain and disability after wrist-hand injury.

Purpose: To explore associations between kinesiophobia and pain catastrophizing with ipsilateral persistent shoulder disability in patients with wrist-hand injury after 6 months.

[Properties of the Test of Early Learning and Development, second edition "TADI", in the context of scale validation policies for children under 6 years of age in Chile and Latin America].

Unlabelled: In Latin America, scales have been built to assess Early Child Development (EChD), most of them for direct evaluations. In Chile, the Psychomotor Development Evaluation Scale (EEDP) and the Test of Psychomotor Development (TEPSI) were developed in the 70's and 80's and are still used in different Spanish-speaking countries, but have not been updated or revalidated. In response to the need for a new instrument built in Chile for the evaluation of EChD on a large scale, the Test of Early Learning and Development (TADI-2, for its acronym in Spanish) was built and standardized between 2009 and 2012.

Integrative multiomics reveals common endotypes across PSEN1, PSEN2, and APP mutations in familial Alzheimer's disease.

Background: PSEN1, PSEN2, and APP mutations cause Alzheimer's disease (AD) with an early age at onset (AAO) and progressive cognitive decline. PSEN1 mutations are more common and generally have an earlier AAO; however, certain PSEN1 mutations cause a later AAO, similar to those observed in PSEN2 and APP.

Methods: We examined whether common disease endotypes exist across these mutations with a later AAO (~ 55 years) using hiPSC-derived neurons from familial Alzheimer's disease (FAD) patients harboring mutations in PSEN1, PSEN2, and APP and mechanistically characterized by integrating RNA-seq and ATAC-seq.

Mechanism of oxygen reduction chemical affinity in NiO/SiO interfaces irradiated with keV energy hydrogen and helium ions for heterostructure fabrication.

Low-energy light ion beams are an essential resource in lithography for nanopatterning magnetic materials and interfaces due to their ability to modify the structure and properties of metamaterials. Here we create ferromagnetic/non-ferromagnetic heterostructures with a controlled layer thickness and nanometer-scale precision. For this, hydrogen ion (H) irradiation is used to reduce the antiferromagnetic nickel oxide (NiO) layer into ferromagnetic Ni with lower fluence than in the case of helium ion (He) irradiation.

Antioxidant PRDX3 gene therapy protects brain cells and prevents neurodegeneration in an animal model of Parkinson's disease.

Neurodegenerative diseases, including Parkinson's Disease (PD), are a significant global health challenge with no effective therapies to counteract neurodegeneration. Genetic and environmental factors lead to mitochondrial dysfunction and increased reactive oxygen species (ROS), resulting in oxidative stress. This stress reduces levels of Peroxiredoxin 3 (PRDX3), a key protein for maintaining ROS balance at the mitochondrial level, increasing the substantia nigra's susceptibility to damage.

Visceral Artery Aneurysms: A 40-Year Experience from a Single Center.

Background: Visceral artery aneurysms (VAAs) are a relatively uncommon phenomenon, and all types of VAA occur at an incidence of 0.01% to 0.2%.

Automated MRI pituitary structured reporting from free-text using a fine-tuned Llama model: a feasibility study.

Background And Objective: Structured reports in radiology have demonstrated substantial advantages over unstructured ones. However, the transition from unstructured to structured reporting can face challenges, as experienced radiologists worry about the potential loss of valuable information. In this study, we fine-tuned the Llama 2 model capable of generating structured pituitary MRI reports from unstructured reports.