Digoxin enhances the effect of antiepileptic drugs with different mechanism of action in the pentylenetetrazole-induced seizures in mice.

The worldwide prevalence of epilepsy with high percentage of multidrug-resistant patients make it urgent to find new approaches to treating, including the use of combinations of classic anticonvulsants with drugs that have an exclusively original mechanism of action, in particular digoxin. The aim of this work was to investigate the influence of low-dose digoxin on the anticonvulsant effect of sodium valproate, topiramate, levetiracetam, phenobarbital and clonazepam. A basic model of pentylenetetrazole-induced seizures in mice was used.

A Novel Series of [1,2,4]Triazolo[4,3-a]Pyridine Sulfonamides as Potential Antimalarial Agents: In Silico Studies, Synthesis and In Vitro Evaluation.

For the development of new and potent antimalarial drugs, we designed the virtual library with three points of randomization of novel [1,2,4]triazolo[4,3-]pyridines bearing a sulfonamide fragment. The library of 1561 compounds has been investigated by both virtual screening and molecular docking methods using falcipain-2 as a target enzyme. 25 chosen hits were synthesized and evaluated for their antimalarial activity in vitro against .

Methylation of Methyl 4-Hydroxy-2-thioxo-1,2-dihydroquinoline-3-carboxylate: Synthetic, Crystallographic, and Molecular Docking Studies.

Consecutive alkylation of 4-hydroxy-2-thioxo-1,2-dihydroquinoline-3-carboxylate by CHI has been investigated to establish regioselectivity of the reaction for reliable design and synthesis of combinatorial libraries. In the first stage, the product of S-methylation-methyl 4-hydroxy-2-(methylthio)quinoline-3-carboxylate was obtained. The subsequent alkylation with CHI led to the formation of both - and -methylation products mixture-methyl 4-methoxy-2-(methylthio)quinoline-3-carboxylate and methyl 1-methyl-2-(methylthio)-4-oxo-1,4-dihydroquinoline-3-carboxylate with a predominance of -methylated product.

Sex Workers Should not Be Forgotten in Africa's COVID-19 Response.

COVID-19 is a global health emergency facing many countries around the world. Sex workers in Africa are among one of the vulnerable populations disproportionately affected by the COVID-19 pandemic on the continent. Sex workers are excluded from African government safety net, and this may force some sex workers back to sex work amid the COVID-19 pandemic.

(1,3)-3-(1-Benzo[]imidazol-2-yl)-1,2,2-tri-methyl-cyclo-pentane-1-carb-oxy-lic acid as a new anti-diabetic active pharmaceutical ingredient.

The chiral title compound, CHNO, which can be used for producing active pharmaceutical ingredients for treatment of type 2 pancreatic diabetes and other pathologies dependent on insulin resistance, was prepared from (1,3)-camphoric acid and -phenyl-enedi-amine. It crystallized from an ethanol solution in the chiral monoclinic space group. The five-membered ring adopts a twisted conformation with the methyl-substituted C atoms displaced by -0.

New and Interesting Fungi. 3.

Seven new genera, 26 new species, 10 new combinations, two epitypes, one new name, and 20 interesting new host and / or geographical records are introduced in this study. New genera are: (based on ) on leaves of (Italy); (based on ) on branch of sp. (Ukraine); (based on ) on trunk of (Ukraine); (based on ) on leaves of (Russia); (based on ) on (Russia); (based on ) on leaves of (Malaysia); (based on ) on leaves of (Russia).

Portable ozone sterilization device with mechanical and ultrasonic cleaning units for dentistry.

A portable device for cleaning and ozone sterilization of small-sized delicate dental instruments that cannot withstand the high heat and humidity of standard autoclaving has been developed. The device contains a remote unit for magnetic mechanical washing, an ultrasonic bath for pre-cleaning treatment, and a container for ozone sterilization with a reactor based on dielectric barrier discharge. The maximum ozone concentration in water reached 8.

LACTOSE TOLERANCE AND RISK OF MULTIFACTORIAL DISEASES ON THE EXAMPLE OF GASTROINTESTINAL TRACT AND BONE TISSUEPATHOLOGIES.

Metabolism changes play a key role in many mammalian diseases such as decreasing bone mineral density, osteoporosis, digestive disorders. Lactose intolerance is found in 75% of the in human world's population. The aim of this study was evaluation of the genetic aspects of lactase deficiency in different ethnic groups and its relation with pathologies of bone tissue and gastrointestinal tract.

Influence of ortho-substituent on the molecular and crystal structures of 2-(N-arylimino)coumarin-3-carboxamide: isotypic and polymorphic structures.

During a comprehensive study of a series of 2-(N-arylimino)coumarin-3-carboxamides with the aryl group substituted in the ortho-position by either a halogen atom, a methyl group or a methoxy group, the existence of three groups of isotypic crystal structures has been revealed. The similarity of crystal structures belonging to the same groups was confirmed by the analysis based on the comparison of pairwise interactions energies obtained from quantum chemical calculations. Group I includes unsubstituted, methyl-substituted and polymorphic modification 1 of fluoro-substituted 2-(N-arylimino)coumarin-3-carboxamide.

Graphene oxide quantum dots immobilized on mesoporous silica: preparation, characterization and electroanalytical application.

Because of its high surface area and combination of various functional groups, graphene oxide (GO) is currently one of the most actively studied materials for electroanalytical applications. It is not practical to utilize self-supported GO on its own and thus it is commonly integrated with different supporting carriers. Having a large lateral size, GO can only wrap the particles of the support and thus can significantly reduce the surface area of porous materials.

Prevalence of non-communicable diseases and its risk factors among Ijegun-Isheri Osun residents in Lagos State, Nigeria: a community based cross-sectional study.

Background: The rapid epidemiologic transition of diseases has adverse implications for low-and middle-income countries (LMICs) like Nigeria due to their limited healthcare, weaker health systems and the westernization of lifestyle. There is a need to evaluate the enormity or otherwise of non-communicable diseases (NCDs) burden in such low resource settings. We performed this survey to determine the prevalence of NCDs and its risk factors among the Ijegun- Isheri Osun community residents of Lagos, Nigeria.

Computing p Shifts Using Traditional Molecular Dynamics: Example of Acid-Base Indicator Dyes in Organized Solutions.

A compound's acidity constant () in a given medium determines its protonation state and, thus, its behavior and physicochemical properties. Therefore, it is among the key characteristics considered during the design of new compounds for the needs of advanced technology, medicine, and biological research, a notable example being pH sensors. The computational prediction of for weak acids and bases in homogeneous solvents is presently rather well developed.

DNA-Specific DAPI Staining of the Pyrenoid Matrix During its Fission in Dunaliella salina (Dunal) Teodoresco (Chlorophyta).

The algal pyrenoid is a naked phase-separated liquid compartment inside the chloroplast consisting predominantly of densely packaged Rubisco and most often transversed by a system of lipid membranes. The pyrenoid participates in carbon-concentrating mechanisms of algae. During the cell division, the daughter cells of algae acquire the pyrenoids via their assembly or fission, the mechanisms of which are not fully understood.

Study of anti-virus actions of metabolites of lactobacteria.

Objective: The aim: of the work was to study the antiviral activity of the metabolites of the probiotic strain Lactobacillus rhamnosus GG (LGG or ATCC 53103) regarding clinical strains of enteroviruses (Coxsackie B-5, ECNO21) isolated from the feces of intestinal infections.

Patients And Methods: Materials and methods: The object of the study was substrate-dependent cell cultures of HeLa, Vero, Hep-2 lines. The titer of the virus was determined by the presence of a clear cytopathic action (CPA) in the monolayer infected cells of the virus.

Concomitant polymorphic forms of 3-cyclopropyl-5-(2-hydrazinylpyridin-3-yl)-1,2,4-oxadiazole.

The dipharmacophore compound 3-cyclopropyl-5-(2-hydrazinylpyridin-3-yl)-1,2,4-oxadiazole, CHNO, was studied on the assumption of its potential biological activity. Two concomitant polymorphs were obtained on crystallization from isopropanol solution and these were thoroughly studied. Identical conformations of the molecules are found in both structures despite the low difference in energy between the four possible conformers.

The comprehensive evaluation of patients' condition in recovery and residual periods of aneurysmal subarachnoid hemorrhage.

Objective: The aim: is to evaluate peculiarities of clinical and neurological characteristics, quality of life, brain morphometry changes and metabolic deviations of patients, who suffered from aneurysmal subarachnoid hemorrhage.

Patients And Methods: Materials and methods: In the period of 2016-2019 we examined 114 patients, who signed the informed consent, taking into account their age, clinical and anatomical form of hemorrhage, disease duration, Hunt-Hess severity grade, complications of acute period. Such parameters were evaluated, as clinical and neurological characteristics, the degree of the Barthel index and the modified Rankin scale, cognitive functioning (MoCA), psycho-emotional sphere and quality of life (HADS, SF-36), morphometric parameters based on brain computed tomography measurements, explored the indicators of apoptosis, mitochondrial dysfunction, intracellular oxidative stress.

Morphological assessment of wound healing after cryodestruction of skin using an aqueous colloidal solution of C60 fullerenes.

Objective: The aim is to assess the morphological state of a cold skin wound in an experiment, using an aqueous colloidal solution of C60 fullerenes.

Patients And Methods: Materials and methods: An experimental study was conducted in 30 six-month-old male rats divided into three groups, 10 individuals in each group: group I included rats that did not undergo any manipulations; group II was represented by rats which underwent a cold wound simulation on the lateral thigh surface; group III was represented by rats who had a simulated cold wound followed by introduction of an aqueous colloidal solution of C60 fullerenes at a dose of 0.1 mg/kg for 5 days into the abdominal cavity, starting from the cryodamage day.

Using of endovascular catheter methods in surgical treatment patients with lung bleeding.

Objective: The aim: Improve the results of the treatment of patients with pulmonary bleeding.

Patients And Methods: Materials and methods: We examined 57patients with lung bleeding different etiology, who were hospitalized in the department of Thoraco-Abdominal Surgery. All patients were divided into two groups - of the main group 27patients and the comparison group 30 patients, depend of ages, sex, nosological form, level of lung bleeding.

Conceptual directions in organization of medical assistance for children in the conditions of education.

Objective: The aim of this work is: to define the Conceptual model of medical provision of children in educational institutions in modern conditions.

Patients And Methods: Materials and methods: analysis of data from a longitudinal study of children's health; questionnaire of parents, teachers, heads of educational institutions on the volume of medical support for students; questioning students' lifestyle and social determinants of health.

Results: Results: According to the study has determined the medical and social determinants which negative affect on health status of school age childrens; detected the most appropriate types of of school health services which based on the parents, medical and teachers opinion.

Enhancing and accelerating stroke treatment in Eastern European region: Methods and achievement of the ESO EAST program.

Introduction: Despite the availability of prevention and therapies of stroke, their implementation in clinical practice, even of low-cost ones, remains poor. In 2015, the European Stroke Organisation (ESO) initiated the ESO Enhancing and Accelerating Stroke Treatment (EAST) program, which aims to improve stroke care quality, primarily in Eastern Europe. Here, we describe its methods and milestones.

The Analysis of Green Areas' Accessibility in Comparison with Statistical Data in Poland.

The study discusses the problem of public green areas' accessibility for the residents of large cities in Poland. The purpose of the research is to assess the possibility of applying the British Accessible Natural Greenspace Standard (ANGSt) method in determining the amount of natural green space available to residents in Polish conditions including, in particular, the assessment of accessibility using data collected by the Central Statistical Office and the verification of results based on detailed research. The identification of green areas for 18 voivodeship cities in Poland was prepared using the GIS programme, taking into account public green space, provided for general access and free of change.

New and Interesting Fungi. 2.

One order, seven families, 28 new genera, 72 new species, 13 new combinations, four epitypes, and interesting new host and / or geographical records are introduced in this study. Pseudorobillardaceae is introduced for (based on ). New genera include: (based on ) on twigs of (Germany); (based on ) on (Ukraine); (based on ) on fallen twigs of (Ukraine); (based on ) on (France) (incl.

The impact of an educational institution on development of healthy lifestyle skills for prevention of obesity in adolescents.

Objective: The aim: To determine that nature of perception of an educational institution has on degree of risk as to development of bad habits in eating and daily routine.

Patients And Methods: Materials and methods: We have conducted surveys among 1015 students of educational institutions, residing in a large industrial center through special questionnaire was developed by SI «ICAHC NAMS». According to answer to the question about their attitude towards school, schoolchildren have been divided into 6 groups.