Phosphorus adsorption on iron-coated sand under reducing conditions.

Mitigation measures are needed to prevent large loads of phosphate originating in agriculture from reaching surface waters. Iron-coated sand (ICS) is a residual product from drinking water production. It has a high phosphate adsorption capacity and can be placed around tile drains, taking no extra space, which increases the farmers' acceptance.

Geographical contrasts of Y-chromosomal haplogroups from wild and domestic goats reveal ancient migrations and recent introgressions.

By their paternal transmission, Y-chromosomal haplotypes are sensitive markers of population history and male-mediated introgression. Previous studies identified biallelic single-nucleotide variants in the SRY, ZFY and DDX3Y genes, which in domestic goats identified four major Y-chromosomal haplotypes, Y1A, Y1B, Y2A and Y2B, with a marked geographical partitioning. Here, we extracted goat Y-chromosomal variants from whole-genome sequences of 386 domestic goats (75 breeds) and seven wild goat species, which were generated by the VarGoats goat genome project.

Composite Gels Containing Whey Protein Fibrils and Bacterial Cellulose Microfibrils.

In this study, we investigated the gelation of WPI fibrils in the presence of bacterial cellulose (BC) microfibrils at pH 2 upon prolonged heating. Rheology and microstructure were investigated as a function of BC microfibril concentration. The presence of BC microfibrils did not influence the gelation dynamics and resulting overall structure of the WPI fibrillar gel.

Computational prediction of protein interfaces: A review of data driven methods.

Reliably pinpointing which specific amino acid residues form the interface(s) between a protein and its binding partner(s) is critical for understanding the structural and physicochemical determinants of protein recognition and binding affinity, and has wide applications in modeling and validating protein interactions predicted by high-throughput methods, in engineering proteins, and in prioritizing drug targets. Here, we review the basic concepts, principles and recent advances in computational approaches to the analysis and prediction of protein-protein interfaces. We point out caveats for objectively evaluating interface predictors, and discuss various applications of data-driven interface predictors for improving energy model-driven protein-protein docking.

Cigarette smoke-induced lung emphysema in mice is associated with prolyl endopeptidase, an enzyme involved in collagen breakdown.

There is increasing evidence that the neutrophil chemoattractant proline-glycine-proline (PGP), derived from the breakdown of the extracellular matrix, plays an important role in neutrophil recruitment to the lung. PGP formation is a multistep process involving neutrophils, metalloproteinases (MMPs), and prolyl endopeptidase (PE). This cascade of events is now investigated in the development of lung emphysema.

A comparison of fixation methods on lung morphology in a murine model of emphysema.

Emphysema is characterized by enlargement of the alveoli, which is the most important parameter to assess the presence and severity of this disease. Alveolar enlargement is primarily defined on morphological criteria; therefore, characterization of this disease with morphological parameters is a prerequisite to study the pathogenesis. For this purpose, different methods of lung fixation were evaluated in a murine model of LPS-induced lung emphysema.

Overtrained horses alter their resting pulsatile growth hormone secretion.

The influence of intensified and reduced training on nocturnal growth hormone (GH) secretion and elimination dynamics was studied in young (1.5 yr) Standardbred geldings to detect potential markers indicative for early overtraining. Ten horses trained on a treadmill for 32 wk in age-, breed-, and gender-matched fixed pairs.

Cell preparation methods influence Escherichia coli D21g surface chemistry and transport in saturated sand.

The effect of cell preparation methods on the surface chemistry and retention of Escherichia coli D21 g was investigated over a range of ionic strength conditions. The cell preparation methods that were considered included filtration and centrifugation (at various speeds and for different durations). For a given ionic strength condition, it was found that cells prepared by filtration were more negatively charged and hydrophobic than cells prepared by centrifugation.

(Over)training effects on quantitative electromyography and muscle enzyme activities in standardbred horses.

Too intensive training may lead to overreaching or overtraining. To study whether quantitative needle electromyography (QEMG) is more sensitive to detect training (mal)adaptation than muscle enzyme activities, 12 standardbred geldings trained for 32 wk in age-, breed-, and sex-matched fixed pairs. After a habituation and normal training (NT) phase (phases 1 and 2, 4 and 18 wk, respectively), with increasing intensity and duration and frequency of training sessions, an intensified training (IT) group (phase 3, 6 wk) and a control group (which continued training as in the last week of phase 2) were formed.

Efficient and reliable schemes for nonlinear diffusion filtering.

Nonlinear diffusion filtering in image processing is usually performed with explicit schemes. They are only stable for very small time steps, which leads to poor efficiency and limits their practical use. Based on a discrete nonlinear diffusion scale-space framework we present semi-implicit schemes which are stable for all time steps.

Image reconstruction by convolution with symmetrical piecewise nth-order polynomial kernels.

The reconstruction of images is an important operation in many applications. From sampling theory, it is well known that the sine-function is the ideal interpolation kernel which, however, cannot be used in practice. In order to be able to obtain an acceptable reconstruction, both in terms of computational speed and mathematical precision, it is required to design a kernel that is of finite extent and resembles the sinc-function as much as possible.

Controls on catchment-scale patterns of phosphorus in soil, streambed sediment, and stream water.

Many models of phosphorus (P) transfer at the catchment scale rely on input from generic databases including, amongst others, soil and land use maps. Spatially detailed geochemical data sets have the potential to improve the accuracy of the input parameters of catchment-scale nutrient transfer models. Furthermore, they enable the assessment of the utility of available, generic spatial data sets for the modeling and prediction of soil nutrient status and nutrient transfer at the catchment scale.

Conversation with a Truth Seeker.

This paper is an invited response to Peter Rudnytsky's "Guardians of Truth" article. Taking issue with what are presented as fundamental theoretical and methodological caveats, this article discusses the question of when and how differing discourses on the history of psychoanalysis may or may not be compatible. In particular the author questions the validity of a concept of truth as defined from within a field of knowledge, to arrive at definitions of discourse and dialogue that can be useful to acquire new forms of knowledge.

Nitrous oxide emission and denitrification in chronically nitrate-loaded riparian buffer zones.

Riparian buffer zones are known to reduce diffuse N pollution of streams by removing and modifying N from agricultural runoff. Denitrification, often identified as the key N removal process, is also considered as a major source of the greenhouse gas nitrous oxide (N2O). The risks of high N2O emissions during nitrate mitigation and the environmental controls of emissions have been examined in relatively few riparian zones and the interactions between controls and emissions are still poorly understood.

Automatic registration of CT and MR brain images using correlation of geometrical features.

Describes an automated approach to register CT and MR brain images. Differential operators in scale space are applied to each type of image data, so as to produce feature images depicting "ridgeness". The resulting CT and MR feature images show similarities which can be used for matching.

Fast SPECT simulation including object shape dependent scatter.

A fast simulator of SPECT projection data taking into account attenuation, distance dependent detector response, and scatter has been developed, based on an analytical point spread function model. The parameters of the scatter response are obtained from a single line source measurement with a triangular phantom. The simulator is able to include effects of object curvature on the scatter response to a high accuracy.

Reversible 3-D decorrelation of medical images.

Two methods, namely, differential pulse code modulation (DPCM) and hierarchical interpolation (HINT), are considered. It is shown that HINT cannot be extended straightforwardly to 3-D images as contrasted with DPCM. A 3-D HINT is therefore proposed which is based on a combination of 2-D and 3-D filters.