Ultraclean air systems and the claim that laminar airflow systems fail to prevent deep infections after total joint arthroplasty.

The World Health Organization published guidelines in 2016 for preventing surgical site infections. The guidelines contained a conditional recommendation that laminar airflow (LAF) ventilation systems should not be used to reduce the risk of infection after total joint arthroplasty (TJA). This recommendation was largely based on a systematic review and meta-analysis of information from hospital infection surveillance registries.

Diabetes treatment and hypoglycaemic episodes in elderly patients at nursing homes in Uppsala County.

Aim: The aim of this study was to examine the situation for elderly patients with diabetes living in nursing homes with regard to diabetes treatment, clinical variables, and vascular complications associated with diabetes. A second aim was to evaluate if the patients were at risk of hypoglycaemia.

Methods: This was a cross-sectional study including diabetes patients from all 30 nursing homes in Uppsala County, Sweden.

The use of closo-dodecaborate-containing linker improves targeting of HNSCC xenografts with radioiodinated chimeric monoclonal antibody U36.

Radionuclide imaging of head and neck squamous cell carcinoma (HNSCC) using monoclonal antibodies (MAbs) has the potential to contribute to improved diagnosis and staging, thereby making more effective treatment possible. Chimeric monoclonal antibody U36 (cMAb U36), specific to CD44v6 antigen, is a candidate for the targeting of HNSCC. The aim of this study was to compare the influence of indirect iodination via closo-dodecaborate-based linker (DABI) with the influence of direct radioiodination on the biodistribution of the chimeric anti-CD44v6 antibody U36.

Radioimmunotherapy with astatine-211 using chimeric monoclonal antibody U36 in head and neck squamous cell carcinoma.

Objectives: In advanced head and neck squamous cell carcinoma (HNSCC), there is a need for an adjuvant treatment. We aim to evaluate the biodistribution and therapeutic effect of radioimmunotherapy using the alpha emitting, astatine-211-labeled, chimeric monoclonal antibody U36 (U36) on the HNSCC cell line UT-SCC7 in vivo.

Study Design: Xenograft tumors were inoculated subcutaneously in nude mice.

Alpha and beta subunits of acetylcholine receptors in the human inner ear.

The localization and distribution of nicotinic acetylcholine receptors (n-ACh-r) was characterized by studying alpha and beta subunits in the adult human inner ear by FITC fluorescence technique. In the cochlea, distinct fluorescence staining occurred for beta subunits in outer hair cells (OHCs), but no alpha subunits were identified. Beta subunits differ quantitatively between the three rows of OHCs, decreasing along a base-to-apex gradient in the cochlea.

Developmental morphology of the middle ear.

The development of the murine middle ear was monitored both qualitatively and morphometrically by scanning electron microscopy from the 19th gestational day to the adult stage. At birth, the middle ear was less well developed than the inner ear. The tympanic membrane (TM) was obscured by occlusion of the external auditory canal.

Auditory epidermal cell migration. V. Transmission electron-microscopic study of tympanic membrane and external auditory canal in the mouse.

The morphology of the tympanic membrane (TM) and external auditory canal (EAC) in mice was examined by transmission electron microscopy, in seven different regions: the handle of the malleus (HM) region, the intermediate and annular regions of the pars tensa (PT), the region of the pars flaccida (PF), the annular and osseous regions of the EAC and the cartilaginous region of the EAC. Each region displayed its own unique findings. In most regions of the TM and EAC-except the intermediate region of the PT-basal layer cells formed cytoplasmic processes (CPs) on their basal surface and adhered to the basement membrane (BM) by means of hemidesmosomes (HDs).

Comparative cytoskeletal analyses of the inner ear in man and the squirrel monkey.

Serially sectioned human and squirrel monkey labyrinths were analyzed with high-resolution light microscopy after using 25 different monoclonal antibodies (mAbs) identifying all three main classes of cytoskeletal proteins. A high degree of similarity was found in labyrinths from man and squirrel monkey. Only 1 of 25 mAbs stained differently between the two species.

Hyaluronic acid as a molecular filter and friction-reducing lubricant in the human inner ear.

Immunofluorescence for hyaluronic acid occurred intracellularly in morphologically highly specialized areas in the adult human inner ear, for instance in the cuticular plates of all types of hair cells, at the apposition between outer hair cells and Deiter's cell bodies and in the near-surface area of Hensen's cells. The cytoskeletal organization in these regions is characterized by tightly packed filamentous proteins. Under physiological stimulus these regions undergo micromechanical change, either actively moving (force generation) or passively vibrating with changes in elasticity.

The human spiral ganglion.

The adult normal human spiral ganglion (SG) was analyzed with regard to ultrastructure and immunohistochemistry. The cytoskeleton of the SG cells was found to comprise F-actin, intermediate filaments (IFs) and microtubules (MTs). The IF subgroups (cytokeratins, Cks; neurofilaments, NFs, vimentin, glial fibrillary acidic proteins, GFAP; desmin) displayed characteristic staining patterns.