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Gene editing of NCF1 loci is associated with homologous recombination and chromosomal rearrangements.
Commun Biol
October 2024
Division of Gene and Cell Therapy, Institute for Regenerative Medicine, University of Zurich (Schlieren Campus), Schlieren, Switzerland.
Article Synopsis
- CRISPR genome editing in disorders like p47-deficient chronic granulomatous disease (CGD) faces challenges due to chromosomal rearrangements caused by multiple similar gene targets on the same chromosome.
- Research identified that interactions between homologous gene sequences led to significant rearrangements after editing the NCF1 gene and its related pseudogenes in human cell models.
- The study emphasized the importance of understanding the genomic context where editing occurs, as the presence of homologous regions can increase the risk of unintended chromosomal changes during the editing process.