Survey of pathogenic bacteria of biofilms in a metropolitan drinking water distribution system.

Bacteria, especially pathogenic bacteria, were detected in order to estimate the safety of drinking water distribution systems (DWDSs). Sixteen biofilms and 12 water samples (six retained and six flowing) were collected from a city DWDS in eastern China. Biofilms were observed using scanning electron microscopy.

Establishing the feasibility of direct observation in the assessment of tics in children with chronic tic disorders.

Behavior analysis has been at the forefront in establishing effective treatments for children and adults with chronic tic disorders. As is customary in behavior analysis, the efficacy of these treatments has been established using direct-observation assessment methods. Although behavior-analytic treatments have enjoyed acceptance and integration into mainstream health care practices for tic disorders (e.

The effects of the naltrexone implant on rodent social interactions and cocaine-induced conditioned place preference.

Two experiments were conducted to determine the behavioral properties of the naltrexone implant on: 1) rodent social interactions; and 2) the appetitive properties of cocaine. Rats were surgically implanted with a naltrexone implant (placebo, 10 or 30 mg) and placed into an open field for the recording of social interactions. The naltrexone implants increased latency to initiate contact and decreased pinning, bouts of grooming, and crawl unders on all 7 days.

Direct voltammetric observation of redox driven changes in axial coordination and intramolecular rearrangement of the phenylalanine-82-histidine variant of yeast iso-1-cytochrome c.

Direct square-wave and cyclic voltammetric electrochemical examination of the yeast iso-1-cytochrome c Phe82His/Cys102Ser variant revealed the intricacies of redox driven changes in axial coordination, concomitant with intramolecular rearrangement. Electrochemical methods are ideally suited for such a redox study, since they provide a direct and quantitative visualization of specific dynamic events. For the iso-1-cytochrome c Phe82His/Cys102Ser variant, square-wave voltammetry showed that the primary species in the reduced state is the Met80-Fe2+-His18 coordination form, while in the oxidized state the His82-Fe3+-His18 form predominates.

Crossed aphasia in a dextral: a test of the Alexander-Annett theory of anomalous organization of brain function.

A case of crossed aphasia is presented in a strongly right-handed 77-year-old white female without history of familial sinistrality or prior neurological illness. She developed a right middle cerebral artery infarction documented by CT and accompanied by obvious clinical signs of a conduction aphasia with some resolution but continuing obvious language defect after 9 weeks in rehabilitation. Comprehensive neuropsychological and aphasia testing suggested anomalous lateralization of phonologic-output aspects of language, emotional prosody, motor planning and body schema modules with usual lateralization of lexical-semantic aspects of language and visuo-spatial functions.

Relationships between cognitive behavioral techniques, temperament, observed distress, and pain reports in children and adolescents during lumbar puncture.

A limited number of studies have examined relationships between temperament and children's/adolescents' responses to painful procedures and have identified several different dimensions of temperament that are related to children's pain response. The focus of these studies was one-time, acute pain experiences, such as immunization and postoperative pain. In this study, children and adolescents' responses to a moderately painful procedure, lumbar puncture, were examined as they related to temperament.

Modulation of ovarian follicle maturation and effects on apoptotic cell death in Holtzman rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in utero and lactationally.

Recent reports have described the reproduction-modulating and endocrine-disrupting effects following exposure to toxic substances such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Herein, we set out (1) to determine whether TCDD exposure exerts detrimental effects on follicle maturation in the Holtzman rat ovary and (2) to determine whether the effects of TCDD are mediated in part via apoptotic cell death. In certain species, dioxin exposure is correlated with reduced fecundity, reduced ovulatory rate, an increased incidence of endometriosis, and various reproductive cancers.

Effects of clozapine and haloperidol on baseline levels of vacuous jaw movements in aged rats.

Some studies have indicated that aged rats have elevated basal levels of vacuous jaw movements and these vacuous jaw movements are exacerbated by classic neuroleptic drugs like haloperidol, but the effects of the atypical antipsychotic clozapine on vacuous jaw movements in aged rats has not previously been studied. Aged rats were administered daily intraperitoneal injections of either haloperidol (0.04, 0.

Synthetic mutants of Clostridium pasteurianum ferredoxin: open iron sites and testing carboxylate coordination.

The entire polypeptide chains for two new Clostridium pasteurianum ferredoxin (Fd) mutants were prepared with the following site-specific substitutions: Cys11Asp and Cys11 alpha-aminobutyric acid (Cys11 alpha-Aba), the latter being a non-naturally occurring amino acid. Standard t-Boc procedures were used for the synthesis and the peptides. The two apoproteins were reconstituted to the 2[4Fe-4S] holoprotein and their spectroscopic, redox and thermal properties were compared with those of native C.

The effects of raclopride on vacuous jaw movements in rats following acute administration.

Classic neuroleptics produce a syndrome of vacuous jaw movements in rats, whereas atypical neuroleptics like clozapine do not. The present study compared the effects of repeated administration of raclopride, clozapine, haloperidol, or vehicle on vacuous jaw movements in rats over a 4-week period. Rats received an IP injection of drug once a day.

In utero and lactational exposure of female Holtzman rats to 2,3,7,8-tetrachlorodibenzo-p-dioxin: modulation of the estrogen signal.

The environmental pollutant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; dioxin) induces severe reproductive defects in male rats when exposure occurs in utero and during lactation. Yet there is currently a paucity of information regarding the effects of this exposure paradigm in females. In the current study, we examine the effects of TCDD during fetal and perinatal development on the estrogen-signaling system in peripubertal female rats.

The effects of cyproheptadine on vacuous jaw movements in rats: a comparison with haloperidol and clozapine.

Classic neuroleptics produce a syndrome of vacuous jaw movements in rats, but atypical neuroleptics such as clozapine do not. This syndrome has been offered as a rapid and inexpensive means of assaying novel antipsychotic compounds for the production of early onset extrapyramidal side effects. Cyproheptadine is a serotonergic antagonist that has been suggested for the treatment of schizophrenia.

Effect of GHRP-6 and GHRH on GH secretion in rats following chronic glucocorticoid treatment.

The aim of our study was to investigate the effects of His-DTrp-Ala-Trp-Phe-Lys-NH2 (GHRP-6) on baseline and growth hormone-releasing hormone (GHRH) stimulated growth hormone (GH) release in conscious, freely-moving rats receiving chronic glucocorticoid treatment. Animals were treated daily for seven days with either vehicle or dexamethasone (dex, 40 micrograms/day). On the day of experimentation, rats received an i.

Assignments of 1H, 15N, and 13C resonances for the backbone and side chains of the N-terminal domain of DNA polymerase beta. Determination of the secondary structure and tertiary contacts.

DNA polymerase beta consists of an N-terminal single-stranded DNA binding domain and a C-terminal catalytic domain separable by mild proteolysis [Kumar et al. (1990) J. Biol.

Sequential proton resonance assignments and metal cluster topology of lobster metallothionein-1.

NMR studies of 111Cd6-MT 1 from lobster have been conducted to determine coordination structure of Cd-thiolate binding in the protein. Sequential proton resonance assignments were made using standard two-dimensional 1H NMR methods. Two-dimensional 1H-111Cd HMQC experiments were then carried out to determine the cadmium-cysteine connectivities in the protein.

Hypothalamic regulation of growth hormone secretion during food deprivation in the rat.

Suppressed pulsatile GH secretion in food-deprived rats has been hypothesized to be due to an increase in hypothalamic somatostatin secretion. We investigated this hypothesis and the role of GHRH in regulating GH secretion during food deprivation using two different models. In experiment one, rats were food deprived for 72h during which time they received a saline infusion (n = 5).

Use of the nursing minimum data set.

The purpose of this study was to obtain information about usage of the Nursing Minimum Data Set (NMDS). Forty-six individuals (44%) who requested the NMDS Data Collection Manual responded to a mailed questionnaire. Most respondents reported actually using the Manual to educate other nurses or to structure nursing documentation.

Neurobehavioral Cognitive Status Examination: its use with unilateral stroke patients in a rehabilitation setting.

The Neurobehavioral Cognitive Status Examination (NCSE) was administered to 12 patients with right unilateral stroke and 12 with left unilateral stroke and to 12 orthopedic, non-brain-dysfunctional patients who were matched in age and education, for the purpose of studying its usefulness in characterizing cognitive deficits in a stroke rehabilitation population. MANOVA demonstrated significant differences (Phillai's trace, p = .007) between the stroke groups and the controls, but not between the unilateral stroke groups.

Effects of chronic intense exercise training on the leukocyte response to acute exercise.

Circulatory leukocytes vary significantly in response to acute bouts of exercise. However, little is known concerning the adaptability of this response to chronic intense exercise training. We investigated the circulating leukocytic response to acute exercise in trained athletes during a 28-day intense exercise training program.

Electromyographic analysis of reach in individuals with cerebral palsy.

Electromyographic analysis in both the time domain (root mean square EMG) and the frequency domain (mean power frequency EMG) of the biceps, triceps, wrist extensors and wrist flexors were analysed in six young cerebral palsied adults and six normal individuals. The subjects sat in a Rifton positioning chair. Each subject's right arm was positioned with the shoulder adducted, the elbow at 90 degrees and the hand resting on the arm rest.