An income over feed cost nutritional grouping strategy.

This study introduces a new nutritional grouping method, OptiGroup, which maximizes milk income over feed cost (IOFC) using a mixed-integer nonlinear programming optimization algorithm. Analyses compared the OptiGroup with the cluster method, the current state-of-the-art nutritional grouping technique. Analyses were performed using cow-level data from 7 Wisconsin dairy farms.

Transition and change in adolescents and young adults with autism: longitudinal effects on maternal well-being.

We investigated how change in the characteristics of 140 adolescents and young adults with an autism spectrum disorder (ASD) would predict subsequent change in maternal well-being and in the quality of the mother-child relationship. Overall patterns of improvement in maternal well-being and mother-child relationship quality were observed during the study. When the son or daughter had declining behavior problems, were prescribed more psychotropic medications, and exited from high school during the study period, mothers' well-being and perception of relationship quality improved to a greater extent.

Psychological distress among latino family caregivers of adults with schizophrenia: the roles of burden and stigma.

Objective: Little research has focused on the mental health of Latino caregivers with a relative with schizophrenia, despite data showing that up to three-quarters of Latino persons with schizophrenia live with their families. This study examined the relation between caregivers' mental health and perceived burden and stigma and characteristics of the patient and caregiver.

Methods: Interviews were conducted in the language of preference (Spanish or English) in Wisconsin, California, and Texas with 85 Latinos caring for an adult with schizophrenia.

Effect of memory support and elicited production on fast mapping of new words by adolescents with Down syndrome.

Purpose: The purpose of this study was to determine whether memory support and elicited production differentially benefited fast mapping of new vocabulary (comprehension, production accuracy, and speed) in adolescents with Down syndrome (DS) compared with typically developing (TD) children matched for syntax comprehension. The study also examined predictors of individual difference within groups.

Method: The ability of 19 adolescents with DS to fast map new noun vocabulary was compared with 18 TD children matched for syntax comprehension.

A common profile of prefrontal cortical activation following exposure to nicotine- or chocolate-associated contextual cues.

Conditioning and learning factors are likely to play key roles in the process of addiction and in relapse to drug use. In nicotine addiction, for example, contextual cues associated with smoking can be powerful determinants of craving and relapse, even after considerable periods of abstinence. Using the detection of the immediate-early gene product, Fos, we examined which regions of the brain are activated by environmental cues associated with nicotine administration, and compared this profile to the pattern induced by cues associated with a natural reward, chocolate.

Radical mechanisms in adenosylmethionine- and adenosylcobalamin-dependent enzymatic reactions.

A class of enzymatic reactions of S-adenosylmethionine (AdoMet) has recently been recognized, in which AdoMet plays a novel role by initiating free radical formation through the intermediate formation of 5'-deoxyadenosine-5'-yl, the 5'-deoxyadenosyl radical. The reactions are in this way related to adenosylcobalamin-dependent processes, which also depend on the formation of the 5'-deoxyadenosyl radical as an intermediate. The mechanisms by which the 5'-deoxyadenosyl radical is generated by the AdoMet- and adenosylcobalamin-dependent enzymes are very different.

Mental retardation research methods in Latino communities.

The importance of understanding specific methods and strategies for conducting mental retardation research in Latino communities is discussed. Research methods used in the recruitment and analysis of a sample of 72 Puerto Rican mothers of a son or daughter with mental retardation are described. The emphasis is on the importance of involving the community in many aspects of the study in order to (a) ensure that the community benefits, (b) strengthen the scientific integrity of the study, and (c) facilitate sample recruitment.

Maternal illusory control predicts socialization strategies and toddler compliance.

This study examined the relation between mothers' perception of their capacity for controlling infant crying and a later measure of parent-toddler social interaction-compliance with parental requests. Fifty-seven mothers participated in (a) a laboratory task when their children were 5 months old to assess mothers' perception of control and (b) a toy-cleanup task when their children were 24 months old to assess toddler compliance. Perception-of-control scores on the laboratory task reflected accuracy of perception and ranged from accurate to greatly overestimating control, with an overestimation referred to as an illusion of control.

Iron-sulfur proteins: ancient structures, still full of surprises.

This article is a survey of the properties and functions of Fe-S proteins under the following headings: sulfur and iron; iron-sulfur clusters; evolution of cofactor use; early observations; complex and extended clusters; sulfur exchange and core interconversions; synthesis and biosynthesis of Fe-S clusters; functions of Fe-S clusters: electron transfer, electron delocalization, spin states and magnetism, covalency of sulfur bonds; non-electron transfer functions of Fe-S clusters: substrate binding and catalysis, regulatory and sensing functions.

The small subunit of carbamoyl phosphate synthetase: snapshots along the reaction pathway.

Carbamoyl phosphate synthetase (CPS) plays a key role in both arginine and pyrimidine biosynthesis by catalyzing the production of carbamoyl phosphate. The enzyme from Escherichi coli consists of two polypeptide chains referred to as the small and large subunits. On the basis of both amino acid sequence analyses and X-ray structural studies, it is known that the small subunit belongs to the Triad or Type I class of amidotransferases, all of which contain a cysteine-histidine (Cys269 and His353) couple required for activity.

Three-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily.

Escherichia coli PurK, a dimeric N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) synthetase, catalyzes the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. Crystallization of both a sulfate-liganded and the MgADP-liganded E. coli PurK has resulted in structures at 2.

Significance of metal ions in galactose-1-phosphate uridylyltransferase: an essential structural zinc and a nonessential structural iron.

Galactose-1-phosphate uridylyltransferase (GalT) catalyzes the reversible transformation of UDP-glucose and galactose-1-phosphate (Gal-1-P) into UDP-galactose and glucose-1-phosphate (Glc-1-P) by a double displacement mechanism, with the intermediate formation of a covalent uridylyl-enzyme (UMP-enzyme). GalT is a metalloenzyme containing 1.2 mol of zinc and 0.

Hydrogen atom exchange between 5'-deoxyadenosine and hydroxyethylhydrazine during the single turnover inactivation of ethanolamine ammonia-lyase.

The early steps in the single turnover inactivation of ethanolamine ammonia-lyase (EAL) from Salmonella typhimurium by hydroxyethylhydrazine (HEH) have been probed by rapid-mixing sampling techniques, and the destiny of deuterium atoms, present initially in HEH, has been investigated by mass spectrometry. The inactivation reaction produces acetaldehyde, the hydrazine cation radical, 5'-deoxyadenosine, and cob(II)alamin (B(12r)) in amounts stoichiometric with active sites. Rapid-mix freeze-quench EPR spectroscopy and stopped-flow rapid-scan spectrophotometry revealed that the hydrazine cation radical and B(12r) appeared at a rate of approximately 3 s(-)(1) at 21 degrees C.

Hydrazine cation radical in the active site of ethanolamine ammonia-lyase: mechanism-based inactivation by hydroxyethylhydrazine.

A study has been made of the mechanism of inactivation of the adenosylcobalamin-dependent enzyme, ethanolamine ammonia-lyase (EAL), by hydroxyethylhydrazine. Incubation of EAL with adenosylcobalamin and hydroxyethylhydrazine, an analogue of ethanolamine, leads to rapid and complete loss of enzymic activity. Equimolar quantities of 5'-deoxyadenosine, cob(II)alamin (B(12r)), hydrazine cation radical, and acetaldehyde are products of the inactivation.

Vowel posture normalization.

A simple normalization procedure was applied to point-parametrized articulatory data to yield quantitative speaker-general descriptions of "average" vowel postures. Articulatory data from 20 English and 8 Japanese speakers, drawn from existing x-ray microbeam database corpora, were included in the analysis. The purpose of the normalization procedure was to minimize the effects of differences in vocal tract size and shape on average postures derived from the raw data.

Stability of nursing home quality indicators over time.

Background: Nursing home quality indicators (QIs) provide a way to support quality assurance and improvement activities and to help ensure that cost savings are based on increased efficiency and not on decreased quality of care.

Objectives: QIs values are expected to change over time. However, to be good indicators of quality, they should be reasonably stable over "short" periods.

Acoustic studies of dysarthric speech: methods, progress, and potential.

(1) The reader will be able to describe the major types of acoustic analysis available for the study of speech, (2) specify the components needed for a modern speech analysis laboratory, including equipment for recording and analysis, and (3) list possible measurements for various aspects of phonation, articulation and resonance, as they might be manifest in neurologically disordered speech.

Influences of pellet markers on speech production behavior: acoustical and perceptual measures.

Peri- and intraoral devices are often used to obtain measurements concerning articulator motions and placements. Surprisingly, there are few formal evaluations of the potential influence of these devices on speech production behavior. In particular, the potential effects of lingual pellets or coils used in x-ray or electromagnetic studies of tongue motion have never been evaluated formally, even though a large x-ray database exists and electromagnetic systems are commercially available.

Resolution of front-back ambiguity in spatial hearing by listener and source movement.

Normally, the apparent position of a sound source corresponds closely to its actual position. However, in some experimental situations listeners make large errors, such as indicating that a source in the frontal hemifield appears to be in the rear hemifield, or vice versa. These front-back confusions are thought to be a result of the inherent ambiguity of the primary interaural difference cues, interaural time difference (ITD) in particular.

Mortality and hormone-related exposures in women with diabetes.

Objective: Hormone-related events and exposures are related to mortality and especially to cardiovascular disease in women. We evaluated whether such exposures influenced risk in a well-defined group of women with diabetes.

Research Design And Methods: Women with younger- and older-onset diabetes who were identified during a population-based study were queried about number of pregnancies, age at menarche, use of oral contraceptives, use of estrogen replacement therapy, and menopausal status at examinations in 1984-1986.

Latent variables, measurement error and methods for analysing longitudinal binary and ordinal data.

We explore structural equations with latent variables for modelling between-individual variability and measurement error in the analysis of longitudinal binary and ordinal data. The structural equation formulation provides insight into the assumptions and differences in interpretation of methods that are popular for longitudinal data analysis. Introducing the concept of continuous latent variables makes it clear that marginal and cluster-specific models differ because their predicted variables are scaled to different standard deviations, and that adjustment for measurement error in the outcome involves a change in scale as well.

Carbamoyl phosphate synthetase: closure of the B-domain as a result of nucleotide binding.

Carbamoyl phosphate synthetase (CPS) catalyzes the production of carbamoyl phosphate which is subsequently employed in the metabolic pathways responsible for the synthesis of pyrimidine nucleotides or arginine. The catalytic mechanism of the enzyme occurs through three highly reactive intermediates: carboxyphosphate, ammonia, and carbamate. As isolated from Escherichia coli, CPS is an alpha, beta-heterodimeric protein with its three active sites separated by nearly 100 A.

Phenylalanine dehydrogenase from Rhodococcus sp. M4: high-resolution X-ray analyses of inhibitory ternary complexes reveal key features in the oxidative deamination mechanism.

The molecular structures of recombinant L-phenylalanine dehydrogenase from Rhodococcus sp. M4 in two different inhibitory ternary complexes have been determined by X-ray crystallographic analyses to high resolution. Both structures show that L-phenylalanine dehydrogenase is a homodimeric enzyme with each monomer composed of distinct globular N- and C-terminal domains separated by a deep cleft containing the active site.