Enhanced Control of Nonlinear Systems Under Control Input Constraints and Faults: A Neural Network-Based Integral Fuzzy Sliding Mode Approach.

Many existing control techniques proposed in the literature tend to overlook faults and physical limitations in the systems, which significantly restricts their applicability to practical, real-world systems. Consequently, there is an urgent necessity to advance the control and synchronization of such systems in real-world scenarios, specifically when faced with the challenges posed by faults and physical limitations in their control actuators. Motivated by this, our study unveils an innovative control approach that combines a neural network-based sliding mode algorithm with fuzzy logic systems to handle nonlinear systems.

Combining thermal model and kinetics: Implications in dynamic simulation of anaerobic digesters.

In this study, ADM1-based kinetics were combined with a thermal model that accounts for various heat transfers inside and through the reactor's boundaries. Computing the energy of bioreactions based on kinetic rates prevented an overestimation of approximately 20% in the heat demand of the heat exchanger, compared to calculations using feedstock degradation heat. This also improved the representation of the digester's thermal and reactional inertia.

Onopordum platylepis (Murb.) Murb. as a novel source of thistle rennet: First application to the manufacture of traditional Italian raw ewe's milk cheese.

In this study, for the very first time, aqueous extracts obtained from flowers of spontaneously grown or cultivated Onopordum platylepis (Murb.) Murb. thistles were used as coagulating agents for the pilot-scale manufacture of Caciofiore, a traditional Italian raw ewe's milk cheese.

How do different intraoral scanners and milling machines affect the fit and fatigue behavior of lithium disilicate and resin composite endocrowns?

The aim of this in vitro study was to evaluate the effect of the combinations of two different intraoral scanners (IOS), two milling machines, and two restorative materials on the marginal/internal fit and fatigue behavior of endocrowns produced by CAD-CAM. Eight groups (n= 10) were considered through the combination of TRIOS 3 (TR) or Primescan (PS) IOS; 4-axes (CR; CEREC MC XL) or 5-axes (PM; PrograMill PM7) milling machines; and lithium disilicate (LD; IPS e.max CAD) or resin composite (RC; Tetric CAD) restorative materials.

Chemical, microbiological, textural, and sensory characteristics of pilot-scale Caciofiore cheese curdled with commercial Cynara cardunculus rennet and crude extracts from spontaneous and cultivated Onopordum tauricum.

The aim of this study was the chemical, microbiological, textural, and sensory characterization of pilot-scale prototypes of an Italian ewe's raw milk cheese (Caciofiore) curdled with commercial Cynara cardunculus rennet, used as a control, and crude extracts obtained from flowers of either spontaneous or cultivated Onopordum tauricum. Hence, the control and experimental cheese prototypes produced in two rounds of cheesemaking trials were assayed, at the end of their 60-day maturation, for the following features: pH, titratable acidity, dry matter, fat, total and soluble nitrogen (TN and SN, respectively), ash, salt, protein, lactose, viable plate counts and composition of the bacterial and fungal populations, color, texture, volatile organic compounds (VOCs), and olfactory attributes by sensory analysis (the latter for the sole prototypes curdled with the commercial rennet and the extract obtained from cultivated O. tauricum).

Single-cell omics: a new perspective for early detection of pancreatic cancer?

Pancreatic cancer is one of the most lethal cancers, mostly due to late diagnosis and limited treatment options. Early detection of pancreatic cancer in high-risk populations bears the potential to greatly improve outcomes, but current screening approaches remain of limited value despite recent technological advances. This review explores the possible advantages of liquid biopsies for this application, particularly focusing on circulating tumour cells (CTCs) and their subsequent single-cell omics analysis.

Assessment of microbial biocontrol agent (BCA) viability to mechanical and thermal stress by simulating spray application conditions.

Background: In order to improve the biological control agent (BCA) efficacy, stress factors threatening the viability of microorganisms during spray application need to be determined. The effect of spray mixture temperature and exposure time on Trichoderma harzianum T 22 and Bacillus amyloliquefaciens QST713 viability were tested. Concurrently the combined effect of mechanical and thermal stress effect on BCA viability were tested at two initial spray mixture temperatures (14 and 25 °C) by simulating a spray application using airblast sprayers featured by different tank capacity and a spray liquid circuit (without and with hydraulic agitation system).

Comparing the accuracy of distinct scanning systems and their impact on marginal/internal adaptation of tooth-supported indirect restorations. A scoping review.

Objective: To summarize the existing scientific evidence on the effect of distinct intraoral (IOS) and extraoral (EOS) scanners in terms of their accuracy for image acquisition and the marginal/internal adaptation of indirect restorations.

Methods: The protocol of this scoping review is available online (https://osf.io/cwua7/).

Characterization of irrigator emitter to be used as solid set canopy delivery system: which is best for which role in the vineyard?

Background: The timely and flexible treatment of solid set canopy delivery systems (SSCDS) is expanding. Laboratory and field trials were conducted to evaluate the performance of three different irrigators (Pulsar™ system and nozzle combination), typically used in anti-frost and irrigation in vineyards/apple orchards, for plant protection product (PPP) delivery in a Guyot-trained trellised vineyard.

Results: Results showed that irrigator setups perform best when matched to the task-flat fan emitters for horizontal spray application (canopy top) and circular emitters for middle and low canopy application.

Field assessment of a newly-designed pneumatic spout to contain spray drift in vineyards: evaluation of canopy distribution and off-target losses.

Background: The efficacy and environmental sustainability of pesticide application largely depend on maximizing target coverage, while minimizing off-target losses. Recently, laboratory-based measurements were used to develop new cannon-type spout to increase the droplet size spectra produced by a pneumatic vineyard sprayer. The study described below evaluated the effectiveness of the new device to reduce off-target losses (both in-field and off-field ground losses), and to distribute an adequate canopy spray.

Spray performance assessment of a remote-controlled vehicle prototype for pesticide application in greenhouse tomato crops.

Intensive horticultural production is a sector seeking to provide high-quality foods by means of safe and sustainable procedures in compliance with regulations. This requires improvements in the spraying technologies since currently plant protection products are applied by means of hand-held equipment due to its lower cost and easy maintenance. In order to fulfil these requirements, a remote-controlled vehicle prototype (ROBOT SPRAY) was used.

Dihydroorotate dehydrogenase inhibitors in anti-infective drug research.

Pyrimidines are essential for the cell survival and proliferation of living parasitic organisms, such as Helicobacter pylori, Plasmodium falciparum and Schistosoma mansoni, that are able to impact upon human health. Pyrimidine building blocks, in human cells, are synthesised via both de novo biosynthesis and salvage pathways, the latter of which is an effective way of recycling pre-existing nucleotides. As many parasitic organisms lack pyrimidine salvage pathways for pyrimidine nucleotides, blocking de novo biosynthesis is seen as an effective therapeutic means to selectively target the parasite without effecting the human host.

Toward a new method to classify the airblast sprayers according to their potential drift reduction: comparison of direct and new indirect measurement methods.

Background: Drift is one of the most important issues to consider for realising sustainable pesticide sprays. This study proposes an alternative indirect methodology for comparative measurements of drift reduction potential (DRP) generated by airblast sprayers, aimed at overcoming the practical inconveniences and drawbacks of standardized ISO 22866:2005. A test bench in the absence of target crop and wind was employed to measure drift potential values (DPVs).

Assessing the influence of air speed and liquid flow rate on the droplet size and homogeneity in pneumatic spraying.

Background: The efficacy of treatments in vineyards largely depends on the necessary balance between leaf coverage and spray drift and, therefore, knowledge about droplet size is of major importance, but scarce scientific information is available on pneumatic spraying, often adopted in this crop. The objective of this work was to obtain the relationships between the droplet size spectra characterization parameters and the main affecting factors in pneumatic nozzles.

Results: Three liquid flow rates (LFR) and four air speeds (AS) were combined in laboratory conditions to assess their influence on the droplet size spectra (D10, D50 and D90), homogeneity (Relative Span Factor, RSF) and driftability (V ) in two different air shear nozzles (cannon-type and hand-type nozzles).

Developing strategies to reduce spray drift in pneumatic spraying in vineyards: Assessment of the parameters affecting droplet size in pneumatic spraying.

Pneumatic sprayers are widely used in vineyards due to their very fine droplet size, which makes the drift risk to become an important problem to be considered. The aim of this study was to assess the effect of the spout diameter at the release point on the spray droplet size and uniformity achieved for different liquid flow rates (LFR) and air flow rates (AFR). A test bench was developed to simulate a real pneumatic sprayer under laboratory conditions, and it was empirically adjusted to match the air pressure conditions as closely as possible to real working conditions.

Cloning, expression, and antigenic characterization of recombinant protein of Mycoplasma gallisepticum expressed in Escherichia coli.

Mycoplasma gallisepticum (MG) is a member of the most important avian mycoplasmas, causing chronic respiratory disease in chickens and leading to important economic losses in the poultry industry. Recombinant technology represents a strategic approach used to achieve highly reliable and specific diagnostic tests in veterinary diseases control: in particular this aspect is crucial for confirming mycoplasma infection and for maintaining mycoplasma-free breeder flocks. In this study, we identified a component of the pyruvate dehydrogenase dihydrolipoamide acetyltransferase (i.