Antioxidant enzyme dynamics suggest the absence of oxidative stress in the canine endometrium across the estrous cycle.

Oxidative stress plays a vital role in female fertility, yet the mechanisms regulating oxidative balance in the canine endometrium remain poorly understood. This study investigates the dynamics of the antioxidant enzyme system in the canine endometrium, focusing on superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GSR), and glutathione-S-transferase (GST), along with thiobarbituric acid reactive substances (TBARS) and total cellular thiols. Enzyme activities were revealed in five different phases of oestrus cycle in 25 dogs, using a spectrophotometric method: anestrus, proestrus, estrus, early diestrus and diestrus (n = 5 per group).

Long-term effects of individual and combined exposure to microplastics and copper in zebrafish hypothalamic-pituitary-gonadal axis - A multi-biomarker evaluation.

Microplastics (MPs) pollution and metal contamination are two prominent environmental stressors with multifaceted implications for aquatic life and ecosystem health. However, the underlying toxicological mechanisms of MPs and metals co-exposure on fish reproduction processes are largely unknown. In this study, zebrafish (Danio rerio) were exposed to MPs (2 mg/L), copper (25 μg/L, Cu25), and their mixture (Cu25 + MPs), for 30 days.

Advanced driving assistance integration in electric motorcycles: road surface classification with a focus on gravel detection using deep learning.

Riding a motorcycle involves risks that can be minimized through advanced sensing and response systems to assist the rider. The use of camera-collected images to monitor road conditions can aid in the development of tools designed to enhance rider safety and prevent accidents. This paper proposes a method for developing deep learning models designed to operate efficiently on embedded systems like the Raspberry Pi, facilitating real-time decisions that consider the road condition.

From soil to surface water: exploring Klebsiella 's clonal lineages and antibiotic resistance odyssey in environmental health.

In the last decade, the presence of resistant bacteria and resistance genes in the environment has been a cause for increasing concern. However, understanding of its contribution to the spread of bacteria remains limited, as the scarcity of studies on how and under what circumstances the environment facilitates the development of resistance poses challenges in mitigating the emergence and spread of mobile resistance factors. Antimicrobial resistance in the environment is considered one of the biggest challenges and threats currently emerging.

Impact of Antimicrobial Resistance of in Urine of Small Companion Animals in Global Context: Comprehensive Analysis.

The isolation of multidrug-resistant (MDR) bacteria from the urinary tracts of pets is increasingly common, particularly in animals with concurrent health conditions. (PA) is one of the most significant antimicrobial-resistant bacteria affecting cats and dogs within the European Union (EU). This study aims to review the prevalence and antimicrobial resistance patterns of PA isolated from urine samples of small animals globally.

Imaging Studies of the Stifle Joint in (Linnaeus, 1771).

Although the stifle joint of wild felines shares several characteristics observed in domestic cats, other characteristics are specific to each species. This study aimed to evaluate the stifle joints of eight , including two young and six adults, through different imaging examinations. All stifles were assessed using radiographs and computed tomography (CT).

Association Between Cytological and Histopathological Diagnoses of Neoplastic and Non-Neoplastic Lesions in Oral Cavity from Dogs and Cats: An Observational Retrospective Study of 103 Cases.

Cytology is an exam frequently used as a diagnostic technique to evaluate animal oral lesions. However, the retrospective studies published comparing cytology and histology in oral pathology are scarce. This study aimed to determining the association between diagnostic cytology and histopathology in neoplastic and non-neoplastic samples from oral cavity lesions of dogs and cats.

The Impact of a Western Diet and Resistance Training in a Rat Model of Mammary Cancer.

This study aimed to investigate the impact of a Western diet and resistance training on cardiac remodeling in a rat model of chemically induced mammary cancer. Fifty-six female Wistar rats were randomly assigned to one of eight experimental groups, evaluating the impact of Western and standard diets, exercise and sedentarism, and the induction of mammary cancer. Mammary cancer was induced via the intraperitoneal administration of -methyl--nitrosourea (MNU) (50 mg/kg) at seven weeks of age.

The Surface Proteome of Bovine Unsexed and Sexed Spermatozoa.

Current sperm sexing methods are costly and largely restricted to cattle, while immunological techniques targeting sex-specific membrane proteins may offer more economical alternatives. To advance these methods, understanding the proteomic differences between the cell membranes of X- and Y-chromosome-bearing spermatozoa is essential. This study aimed to characterize the cell surface proteome of bovine sperm and identify potential targets for sperm sexing through LC-MS/MS analysis.

The Role of Elderberry Hydrolate as a Therapeutic Agent in Palliative Care.

Elderberry hydrolate, derived from the berries of , has gained attention for its therapeutic properties, particularly in skincare. This review explores its potential applications in palliative care, where patients often experience compromised skin health due to illness or treatment. The bioactive compounds in elderberry hydrolate, including phenylacetaldehyde, 2-acetyl-pyrrole, n-hexanal, furfural, and ()-beta-damascenone, contribute to its anti-inflammatory, antioxidant, antimicrobial, and skin-healing effects.

The Interactive Dynamics of Pseudomonas aeruginosa in Global Ecology.

Pseudomonas aeruginosa is an opportunistic bacterium widely distributed in both natural and urban environments, playing a crucial role in global microbial ecology. This article reviews the interactive dynamics of P. aeruginosa across different ecosystems, highlighting its capacity for adaptation and resistance in response to environmental and therapeutic pressures.

Examining the impact of different re-warm-up strategies on non-starter basketball players' physical performance.

This study assessed the effectiveness of re-warm-up strategies in counteracting performance declines in non-starter female basketball players during prolonged inactivity. Fifteen elite youth players (16.7 ± 0.

Physicochemical dataset from Lima and Douro estuaries (Northwest Portugal).

This article presents a comprehensive dataset of physicochemical data from two urban estuaries on the northern Portuguese coast, based on five sampling campaigns conducted between October 2021 and November 2022. The dataset includes: a) vertical profiles of water physicochemical parameters (temperature, salinity, pH, turbidity, dissolved oxygen concentration and saturation) collected at 8 sampling stations along the Lima estuary (during both ebb and flood tides) and at 11 stations along the Douro estuary (during flood tides); and b) chemical analyses data, including nutrients (nitrate, nitrite, phosphate, ammonium, and silica), chlorophyll , total particulate matter, particulate organic matter, and key metal (copper, zinc, cadmium, iron, nickel, lead, manganese, and chromium) concentrations. For the Lima estuary, additional data on dissolved metals concentrations are provided, offering a detailed picture of metal contamination.

Insecticidal nanoformulations based on a lipid matrix: Physicochemical properties and their effect on non-target aquatic biota.

The incorporation of lambda-cyhalothrin (LC) in lipid nanoparticles (LN) could be a sustainable strategy to increase its efficacy and decrease its hazard to the environment. The purpose of the present work was to perform the interaction between LC and LN after nanoencapsulation and to evaluate their effect on species from different aquatic trophic levels such as Aliivibrio fischeri, Raphidocelis subcapitata, Lemna minor, and Daphnia magna. LN loaded with LC (LN-LC) were produced by green and simple methodology without organic solvents using Precirol ATO5® and Capryol 90® as solid and liquid lipids, respectively, and soy lecithin and TEGO® Care as emulsifiers.

Recent animal models of bladder cancer and their application in drug discovery: an update of the literature.

Introduction: Bladder cancer presents a significant health problem worldwide, with environmental and genetic factors contributing to its incidence. Histologically, it can be classified as carcinoma in situ, non-muscle invasive and muscle-invasive carcinoma, each one with distinct genetic alterations impacting prognosis and response to therapy. While traditional transurethral resection is commonly performed in carcinoma in situ and non-muscle invasive carcinoma, it often fails to prevent recurrence or progression to more aggressive phenotypes, leading to the frequent need for additional treatment such as intravesical chemotherapy or immunotherapy.

Mapping, characterisation, and analysis of initiatives with transformative capacity: A scoping review.

The increasing complexity of socio-environmental vulnerabilities and inherent challenges have led scholars to study nonlinear systemic societal changes. Approaches to address these challenges range from adaptation to the predominant system, to the reconfiguration of the social and ecological life system. The study identifies the key characteristics, dynamics and contextual features of transformative initiatives and assesses their potential to challenge the dominant system.

Pseudomonas aeruginosa: One Health approach to deciphering hidden relationships in Northern Portugal.

Aims: Antimicrobial resistance in Pseudomonas aeruginosa represents a major global challenge in public and veterinary health, particularly from a One Health perspective. This study aimed to investigate antimicrobial resistance, the presence of virulence genes, and the genetic diversity of P. aeruginosa isolates from diverse sources.

Current Biological Insights of Mill. to Improve Crop Sustainability to Climate Change.

The sustainability of agriculture is seriously threatened by climate change. In Europe, chestnut ecosystems, which are growing mainly in Mediterranean climate, are facing during summertime increasing of heat and drought stresses. These induce fragilities on trees, leading to a reduction in productivity and predisposing them to pest and disease attacks.

Anti-Inflammatory, Anti-Hyperglycemic, and Anti-Aging Activities of Aqueous and Methanolic Fractions Obtained from Bouché Fruit Pulp and Peel Extracts.

The genus comprises various species that are globally consumed and that are commonly used for their nutritional value but also for medicinal applications. Within the genus can be found Bouché, a species that is understudied regarding its potential value for the food industry, as a functional food, and for the pharmaceutical industry, as a source of nutraceuticals. Therefore, in this study we investigated the phytochemical composition and bioactivities of aqueous (AF) and methanolic (MF) fractions of pulp and peel hydroethanolic (HE) extracts.

mRNA Degradation as a Therapeutic Solution for Mucopolysaccharidosis Type IIIC: Use of Antisense Oligonucleotides to Promote Downregulation of Heparan Sulfate Synthesis.

Mucopolysaccharidosis type IIIC is a neurodegenerative lysosomal storage disorder (LSD) characterized by the accumulation of undegraded heparan sulfate (HS) due to the lack of an enzyme responsible for its degradation: acetyl-CoA:α-glucosaminide N-acetyltransferase (HGSNAT). Classical treatments are ineffective. Here, we attempt a new approach in genetic medicine, genetic substrate reduction therapy (gSRT), to counteract this neurological disorder.

Effects of strength training and detraining considering maturity status in youth highly trained basketball players.

The study analyzed whether maturation affects young basketball players' strength training and detraining adaptations. Thirty-five youth male basketballers (U-13 to U-15) performed eight weeks of strength training twice weekly, followed by eight weeks without strength training, maintaining their basketball practices. Changes in performance were assessed in three maturity groups based on years from/to age of peak height velocity (PHV): pre-PHV (-1.

New insights on antibacterial mode of action of blue-light photoactivated berberine and curcumin-antibiotic combinations against Staphylococcus aureus.

Antimicrobial photodynamic inactivation (aPDI), using photosensitisers in combination with antibiotics, is a promising multi-target strategy to address antibiotic resistance, particularly in wound infections. This study aimed to elucidate the antibacterial mode of action of combinations of berberine (Ber) or curcumin (Cur) with selected antibiotics (Ber-Ab or Cur-Ab) under blue light irradiation (420 nm) against Staphylococcus aureus, including methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains. Multiple physiological parameters were assessed using complementary assays (fluorometry, epifluorescence microscopy, flame emission and atomic absorption spectroscopy, zeta potential, flow cytometry, and the plate agar method) to examine the effect on ROS production, membrane integrity, DNA damage, motility and virulence factors of S.

Canine lung carcinoma-A descriptive review.

Primary lung cancer is a relatively uncommon tumor in dogs, accounting for about 1% of all canine malignancies. Clinical signs in affected dogs can range from being asymptomatic to exhibiting chronic cough, and in rare cases, dyspnoea. Surgical excision of the primary tumor, along with resection of the affected locoregional lymph nodes is the preferred treatment option for most cases.