Inadequacy of high K+/nigericin for calibrating BCECF. II. Intracellular pH dependence of the correction.

In the accompanying study [G. Boyarsky, C. Hanssen, and L.

Inadequacy of high K+/nigericin for calibrating BCECF. I. Estimating steady-state intracellular pH.

Intracellular pH (pHi) was measured in single vascular smooth muscle (VSM) cells, cultured from rabbit abdominal aorta, using 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) on a microscope-based fluorescence system. Three lines of evidence are presented that using nigericin along with high external K+ to calibrate intracellular BCECF produces systematic errors in pHi. 1) The intrinsic buffering power (beta int), measured using weak bases (e.

Superiority of in vitro over in vivo calibrations of BCECF in vascular smooth muscle cells.

We measured intracellular pH (pHi) in single vascular smooth muscle cells (VSM) cultured from rabbit abdominal aorta, using 2',7'-biscarboxyethyl-5(6)carboxyfluorescein (BCECF) on a microscope-based fluorimetric system. We previously found substantial errors introduced by using high K+/nigericin to calibrate intracellular BCECF (1). We also previously demonstrated that the necessary correction (pHcor) to the high K+/nigericin-calibrated pHi was linearly dependent on pHi, increasing with increasing pHi (2).

Current treatment of severely burned patients.

Objective: The authors provide an update on a multidisciplinary approach to the treatment of severely burned patients. A review of studies and clinical trials from the past to the present include fluid resuscitation, sepsis, immune function, hypermetabolism, early excision, wound healing, scar formation, and inhalation injury.

Summary Background Data: Advances in treating initial burn shock, infection control, early wound closure, and modulation of the hypermetabolic response have decreased morbidity and mortality in the last two decades.

Alanine kinetics in humans during low-intensity exercise.

There is little doubt that pyruvate contributes to the increased alanine flux in exercise, but the role of protein breakdown is less clear. To quantify the relative contributions of pyruvate and protein breakdown to the increase in alanine flux observed in exercise, we used a primed, constant infusion of 15N-alanine and 1-13C-lactate. The rate of appearance of alanine, the de novo synthesis of alanine, and rate of alanine release from protein breakdown were determined in five healthy subjects at rest and during exercise.

Modulation of cardiac sarcoplasmic reticulum ryanodine receptor by sphingosine.

Excitation contraction (EC) coupling in muscle cells involves the movement of calcium through the calcium release channel of the sarcoplasmic reticulum (SR) membrane known as the ryanodine receptor. We have recently shown that the novel second messenger, sphingosine, can block calcium release from skinned skeletal muscle fibers and from isolated skeletal muscle SR membranes (Sabbadini et al., J Biol Chem 267: 15475-15484, 1992).

Alteration in the coding potential and expression of H-ras in human cytomegalovirus-transformed cells.

CMV-transformed cell lines demonstrated a greater level of H-ras RNA (7.5- to 9.5-fold) relative to the level for H-ras in parental cells.

Role of nerve growth factor in oxidant homeostasis: glutathione metabolism.

Free radicals are generated in the CNS by ongoing oxygen metabolism and biological events associated with injury and inflammation. Increased free radical levels may also persist in some chronic neurological diseases and in the aged. Nerve growth factor (NGF) is a member of the neurotrophin family of proteins that can regulate neuronal development, maintenance, and recovery from injury.

Afferent activity in the external branch of the superior laryngeal and recurrent laryngeal nerves.

We investigated the presence of respiratory-modulated receptors in the recurrent laryngeal nerve (RLN) and the external branch of the superior laryngeal nerve (ExtSLN) in anesthetized, spontaneously breathing dogs. Of 39 receptors recorded from the ExtSLN, the vast majority responded with a slowly adapting discharge to compression of the cricothyroid muscle, and only 1 responded to probing of the laryngeal mucosa. Ten receptors showed a respiratory modulation.

Characteristics of successful and unsuccessful applicants to orthopedic residency training programs.

In 1985 there were 288 applicants for the ten postgraduate Year 1 orthopedic residency positions in Galveston and San Antonio, of which 119 (41%) applicants successfully obtained a residency position in one of 76 orthopedic programs approved by the Accreditation Council for Graduate Medical Education. Successful applicants were younger and had higher Medical College Admissions Test scores, National Board of Medical Examiners, Part I (NBME-I) scores, and medical school grade point averages than unsuccessful applicants. Variables that increased acceptance rates were medical student status, an NBME-I score above 500, class rank in the top 40%, and membership in Alpha Omega Alpha honor society.

Left ventricular aneurysm in short axis: a comparison of magnetic resonance, ultrasound and thallium-201 SPECT images.

Short axis magnetic resonance images of a left ventricular aneurysm were compared to similar views obtained by echocardiography and by thallium-201 single photon emission computed tomography. Images of the dyskinetic left ventricular apex and the contractile left ventricular base were analyzed and compared. Unlike the previously reported orthogonal plane magnetic resonance images, short axis imaging provided representative and quantitative information in views comparable to those obtained by standard noninvasive imaging techniques.