Precision oncology platforms: practical strategies for genomic database utilization in cancer treatment.

In recent years, the expansion of molecularly targeted cancer therapies has significantly advanced precision oncology. Parallel developments in next-generation sequencing (NGS) technologies have also improved precision oncology applications, making genomic analysis of tumors more affordable and accessible. Targeted NGS panels now enable the rapid identification of diverse actionable mutations, requiring clinicians to efficiently assess the predictive value of cancer biomarkers for specific treatments.

Influence of photobiomodulation and radiofrequency on the healing of pressure lesions in mice.

The objective of this study was to ascertain the impact of photobiomodulation and radiofrequency on the healing of pressure injuries in mice. A total of 70 animals were randomly assigned to seven experimental groups. A pressure injury was induced in the dorsal region of the mice by the application of two magnets.

In Vitro Wear of Human Enamel Against Monolithic Zirconia After Staining, Glazing and Polishing Treatments.

Objective: To evaluate the effect of staining, glazing, and polishing of stabilized zirconia with 5 mol% of yttrium oxide (5Y-TZP) on the wear behavior of opposing tooth enamel.

Materials And Methods: The plane specimens of 5Y-TZP were divided into 6 groups (n = 10), according to surface treatment: as sintered, staining, glazing, polishing, staining followed by glazing, and staining followed by polishing, and positioned against tooth enamel during the two-body wear test (20 N, 2 Hz, until completing 300,000 cycles). The wear rates of tooth enamel were evaluated using a profile projector and a digital pachymeter as a measure of vertical height loss.

Perches used as environmental enrichment influence fast-growth broilers' biomechanics and locomotor morphometry at the age of 42 days.

Currently available conventional breeding methods for broilers often result in impaired biomechanics and skeletal growth for the animals. The addition of environmental enrichment is an alternative which can help alleviate these effects. This study examines the effects of environmental enrichment on biomechanics, morphometry, and bone mass of broilers across various age groups.

Continuous Meniscal Repair Technique Allows for Shorter Operative Time and Learning Curve Compared With Traditional Vertical Mattress Technique in Controlled Arthroscopic Training in Porcine Model.

Purpose: To compare the amount of time used to perform meniscal suturing on a standardized lesion using either a traditional or continuous arthroscopic suturing technique.

Methods: A preclinical study was carried out with 21 medical doctors who underwent training in the 2 modalities of meniscal repair by arthroscopy in an animal model laboratory. Participants performed both types of sutures with a previously standardized lesion.

Repurposing doxycycline for Alzheimer's treatment: Challenges from a nano-based drug delivery perspective.

Drug repurposing, also known as drug repositioning, involves identifying new applications for drugs whose effects in a disease are already established. Doxycycline, a broad-spectrum antibiotic belonging to the tetracycline class, has demonstrated potential activity against neurodegenerative diseases like Alzheimer's and Parkinson's. However, despite its promise, the repurposed use of doxycycline encounters challenges in reaching the brain in adequate concentrations to exert its effects.

Oxidative stress and mitochondrial dysfunction contributes to postoperative cognitive dysfunction in elderly rats dependent on NLRP3 activation.

Postoperative cognitive dysfunction (POCD), a complication following procedures such as orthopedic surgery, is associated with a worsened prognosis, especially in the elderly population. Several mechanisms have been proposed for communication between the immune system and the brain after surgery. In an experimental tibial fracture (TF) model, we aimed to understand the role of the NLR family pyrin domain containing 3 (NLRP3) on oxidative stress and mitochondrial dysfunction as mechanisms underlying POCD in aged and adult rats.

Microbiological profile of patients with generalized gingivitis undergoing periodontal therapy and administration of Bifidobacterium animalis subsp. lactis HN019: A randomized clinical trial.

Objective: To evaluate the adjunctive use of the probiotic Bifidobacterium animalis subsp. lactis HN019 (B. lactis HN019) to conventional therapy on clinical and microbiological parameters in patients with generalized gingivitis.

Predictive modeling of gestational weight gain: a machine learning multiclass classification study.

Background: Gestational weight gain (GWG) is a critical factor influencing maternal and fetal health. Excessive or insufficient GWG can lead to various complications, including gestational diabetes, hypertension, cesarean delivery, low birth weight, and preterm birth. This study aims to develop and evaluate machine learning models to predict GWG categories: below, within, or above recommended guidelines.

Ethnic Rhinoplasty in the Brazilian Population: Current Concepts.

Introduction: The large mixture of races in Brazil gives the nose its distinctive composition, which makes rhinoplasty difficult for these patients. Every patient should have their anatomy thoroughly examined on an individual basis, as there is no unique strategy that works for all situations.

Methodology: This is a narrative overview of the last 10 years' worth of literature that describes the ethnic traits of each nose, with a particular emphasis on the Brazilian nose, and talks about the major surgical approaches that can be applied in these situations.

β-Tricalcium phosphate incorporated natural rubber latex membranes for calvarial bone defects: Physicochemical, in vitro and in vivo assessment.

Natural rubber latex membrane (NRL) is a biocompatible macromolecule that stimulates angiogenesis and promotes bone repair. Similarly, β-tricalcium phosphate (β-TCP) is an osteoconductive and osteoinductive bioceramic widely used as a bone substitute. Here, we investigated the combined use of these biomaterials in the guided bone regeneration process for calvarial defects in rats.

Efficiency of infrared pyrometer and infrared thermography for assessing body surface temperature in hair sheep.

The objectives of this study were: (i) to verify and compare the coat surface temperature (CST) of regions of interest (ROI) measured through infrared pyrometer (IRP) and thermography (IRT) under two environmental conditions, to evaluate (ii) reliability and (iii) sensitivity of the two infrared equipments to the environmental effect. Twenty hair ewes (Ovis aries), multiparous, non-lactating, non-pregnant, with body weight 59.38 ± 6.

O-Ring and Bar-Clip: A Comparative Analysis of Retention in Overdenture Prostheses - A Systematic Review.

Introduction: Mandibular implant-supported overdentures (IODs) show higher retention than conventional complete dentures. However, there is no consensus on the best attachment to ensure resistance to vertical displacement. The most used are o-ring and bar-clip.

Novel hook plate for radial semilunar lip facet fragment fixation: a finite element analysis.

Intra-articular fractures of the distal radius require anatomical reduction and stable fixation. When the fracture encompasses the articular facet of the bone, maintaining the reduction is challenging due to the fragment's size and high instability. While specific implants have been developed to fix this fragment, their effectives have been limited.

Good Practices in Animal Research: A Web-Based Platform for Training in Laboratory Rodent Experimental Procedures.

The advancement of technology has revolutionized education, particularly through video-based learning. In response, the Good Practices in Animal Research (BPEA, "Boas Práticas em Experimentação Animal" in Portuguese) platform was established as a contemporary educational tool for training in laboratory rodent experimental techniques. Designed to replace traditional animal-centered teaching methods, BPEA provided scientifically accurate video content tailored for veterinary medicine students.

Latin American Natural Product Database (LANaPDB): An Update.

Natural product (NP) databases are crucial tools in computer-aided drug design (CADD). Over the past decade, there has been a worldwide effort to assemble information regarding natural products (NPs) isolated and characterized in certain geographical regions. In 2023, it was published LANaPDB, and to our knowledge, this is the first attempt to gather and standardize all the NP databases of Latin America.

Interaction of graphene oxide with tannic acid: computational modeling and toxicity mitigation in .

Graphene oxide (GO) undergoes multiple transformations when introduced to biological and environmental media. GO surface favors the adsorption of biomolecules through different types of interaction mechanisms, modulating the biological effects of the material. In this study, we investigated the interaction of GO with tannic acid (TA) and its consequences for GO toxicity.

Proteinuria is associated with worse outcomes in babies born to mothers with preeclampsia: A retrospective cohort study at a tertiary referral hospital in Brazil.

To evaluate whether the presence of proteinuria, although no longer mandatory in the revised diagnostic criteria, results in worse maternal and fetal outcomes in preeclampsia (PE). A retrospective cohort study was conducted, analyzing data from pregnant patients diagnosed with PE between January 2015 and December 2019 at a tertiary care center in Brazil. Ethical approval was obtained, and the patient records were reviewed to assess maternal and perinatal outcomes based on the revised diagnostic criteria by the College of Obstetricians and Gynecologists, focusing on the presence or absence of proteinuria.

Kinematic Analysis of Reaching Tasks at Different Execution Speeds in Children with Neonatal Brachial Plexus Palsy.

The aim of this study was to compare the kinematics of reaching tasks at different speeds between children with neonatal brachial plexus palsy (NBPP) and unaffected controls. This cross-sectional study included thirteen children with NBPP (10 ± 2 years old, of which six had upper Erb's palsy and seven had extended Erb's palsy) matched for age and sex with thirteen unaffected controls. Kinematic data were acquired using a Motion Monitor unit with a 3D motion tracking electromagnetic system (Liberty, Polhemus).

Novel apocynin regulates TRPV1 activity in the trigeminal system and controls pain in a temporomandibular joint neurogenic model.

Objective: Herein, we investigate the potential analgesic effect of a newly synthesized chalcone-derived apocynin in a neurogenic pain model.

Methods: Molecular docking was used to foretell the apocynin binding features and dynamics with the TRPV1 channel, and the activity was tested in vitro, using transfected HEK 293T cells with the rat TRPV1 receptor. The analgesic effect of apocynin was investigated using a capsaicin-induced pain model.

Brazilian red propolis reduces the adhesion of oral biofilm cells and the Toxoplasma gondii intracellular proliferation.

Infectious diseases remain as a significant cause of thousands of deaths annually worldwide. Therefore, this study aimed to investigate the antimicrobial and antiparasitic activity of the crude hydroalcoholic extract and compounds isolated from Brazilian Red Propolis (BRP) against oral pathogens and Toxoplasma gondii, using in vitro, in vivo and in silico approaches. Antimicrobial and synergistic activities were determined using the broth dilution method and the checkerboard assay, respectively.

Rheological properties, microstructure, and encapsulation efficiency of inulin-type dietary fiber-based gelled emulsions at different concentrations.

Gelled emulsion systems offer promising matrices for encapsulating bioactive compounds, enhancing stability, bioavailability, and controlled release. Incorporating inulin-type dietary fibers into emulsion-filled gels can innovate food products. This study explored the impact of inulin concentration (0-15 % w/w) on visual aspect, microstructure, particle size distribution, creaming stability, rheological behavior, and encapsulation efficiency of emulsions and gelled emulsions with clove bud oil rich in eugenol.

Short-term exercise counteracts accelerated ageing impacts on physical performance and liver health in mice.

Senescence impairs liver physiology, mitochondrial function and circadian regulation, resulting in systemic metabolic dysregulation. Given the limited research on the effects of combined exercise on an ageing liver, this study aimed to evaluate its impact on liver metabolism, circadian rhythms and mitochondrial function in senescence-accelerated mouse-prone 8 (SAMP8) and senescence-accelerated mouse-resistant 1 (SAMR1) mice. Histological, reverse transcription quantitative polymerase chain reaction (RT-qPCR) and immunoblotting analyses were conducted, supplemented by transcriptomic data sets and AML12 hepatocyte studies.