Characterization of a novel acidic phospholipase A isolated from the venom of Bothrops mattogrossensis: From purification to structural modeling.

Phospholipases A (PLAs) are highly prevalent in Bothrops snake venom and play a crucial role in inflammatory responses and immune cell activation during envenomation. Despite their significance, the specific role of PLAs from Bothrops mattogrossensis venom (BmV) in inflammation is not fully understood. This study sought to isolate and characterize a novel acidic PLA from BmV, designated BmPLA-A, and to evaluate its effects on human umbilical vein endothelial cells (HUVECs), with a specific focus on cytotoxicity, adhesion, and detachment.

Analyses of the Genetic Diversity and Population Structures of spp. Clinical Isolates from Paraíba, Brazil.

Unlabelled: Sporotrichosis is a subcutaneous mycosis of global distribution, capable of affecting both humans and animals, and caused by species of the genus spp. This study aimed to evaluate the genetic diversity and mating type distribution of clinical isolates of human sporotrichosis in Paraíba, Brazil, to better understand the population structure, epidemiology, and diversification of this pathogen, as well as to explore possible transmission routes.

Methods: A total of 36 clinical isolates were morphologically identified, and clinical and demographic data were collected.

Association between food insecurity and overweight: Protocol for a systematic review based on intersectionality of gender and race/color.

Food insecurity violates the right to regular access to quality food, affecting population groups unequally. In Brazil, FI is associated with both malnutrition and increased obesity and is intertwined with racial and gender inequalities, perpetuating cycles of poverty and social exclusion. This protocol aims to select observational studies that evaluate the association between food insecurity and overweight and their intersectional discussions (gender and race/color).

Skin temperature and anthropometric measurements during pregnancy: A cross-sectional study.

Introduction: Pregnancy comprises a period of 41 weeks, in which the female body undergoes several physiological, hormonal and anatomical changes that can generate changes in skin temperature.

Objective: To describe the thermal profile of pregnant women during the first, second and third trimester of pregnancy.

Method: This is a cross-sectional observational study.

Repair with suture tape augmentation vs. reconstruction for ACL tears: A systematic review and meta-analysis.

Background And Aims: Interest in repairing ruptured anterior cruciate ligaments (ACL) has resurged, with suture tape augmentation (internal brace, ACL-IB) emerging as a prominent technique. However, the efficacy of ACL-IB compared to ACL reconstruction (ACL-R) remains unclear. We conducted a meta-analysis to address this gap.

Physical activity patterns in adolescents: A longitudinal study.

The present study aimed at describing and comparing the physical activity (PA) patterns of adolescents from a middle-income country over a 4-year period. This is a longitudinal observational study (four waves of data collection, 2014-2017) with 668 adolescents aged 10-13 years in 2014 (mean age of 11.8 ± 0.

Impact of the Metal-Organic Frameworks Polymorphism on the Electrocatalytic Properties of CeO toward Oxygen Evolution.

Hydrogen (H) is a viable alternative as a sustainable energy source, however, new highly efficient electrocatalysts for water splitting are still a research challenge. In this context, metal-organic frameworks (MOFs)-derived nanomaterials are prominent high-performance electrocatalysts for hydrogen production, especially in the oxygen evolution reaction (OER). Here, a new synthesis of two cerium oxide (CeO) electrocatalysts using Ce-succinates MOFs as templates is proposed.

Analysis of dental anatomy knowledge among dental students: A preliminary study.

Objective: This study aimed to compare the level of dental anatomy knowledge and visual acuity of dental students throughout their undergraduate course.

Materials And Methods: This descriptive, observational, and cross-sectional study with a quantitative approach was conducted over one year. Thirty-seven students, divided into three groups based on the time spent studying dental anatomy and carving, had their theoretical knowledge and visual acuity assessed using two questionnaires.

Assessment of clot-lysing and membrane-stabilizing capacity of ascorbic acid: approach with molecular docking.

This study aimed to evaluate the clot-lysing and membrane stabilizing capacities of ascorbic acid (AA) using and methods. For this, we used clot lysis and hemolyzing tests to check the anti-atherothrombosis and membrane-stabilizing properties of AA, respectively. Additionally, molecular docking studies were performed to investigate AA's interactions with cyclooxygenase-1 (COX-1) and plasminogen enzymes.

Antiviral and immunomodulatory effects of ouabain against congenital Zika syndrome model.

Zika virus (ZIKV) is an arbovirus associated with neurological disorders accompanying congenital infections. With no vaccine or antiviral approved, there is an urgent need for the development of effective antiviral agents against ZIKV infection. We evaluated the anti-ZIKV and immunomodulatory activity of ouabain, a Na/K-ATPase inhibitor known to have immunomodulatory and antiviral activities, using human neural stem and progenitor cells (hNS/PCs) and a murine model of congenital Zika syndrome (CZS).

Gut Dysbiosis and Probiotic Therapy in Chronic Kidney Disease: A Comprehensive Review.

Chronic kidney disease (CKD) is a multifactorial disease affecting more than 13.4% of the world's population and is a growing public health problem. It is silent in its early stages and leads to irreversible kidney damage as the disease progresses.

New cave species of Cyphoderus Nicolet and Pararrhopalites Bonet & Tellez (Hexapoda, Collembola) from Caatinga biome, Brazil.

In recent years, surveys have revealed a high diversity of troglophilic and troglobitic species of Brazilian Collembola, mainly represented by the Entomobryidae and Sminthuridae, which hold some representative genera in Brazilian caves such as Cyphoderus Nicolet and Pararrhopalites Bonet & Tellez. Here we describe in detail two new species of these genera sampled from caves of the semiarid region of Rio Grande do Norte state, Brazil. Cyphoderus caatinguensis sp.

Thermal and storage stability of novel nutraceuticals combining potential probiotic Limosilactobacillus fermentum strains and freeze-dried jabuticaba [Myrciaria cauliflora (Mart.) O. Berg] peel.

This study evaluated the stability of novel nutraceuticals containing jabuticaba freeze-dried peel (FJP) and a mix of potential probiotic L. fermentum strains [139, 263 and 296 (LfM)] (JM: FJP + LfM; JFM: FJB + LfM + fructooligosaccharides) through determination of thermal stability, viable cell counts, bacterial physiological status, phenolic compound contents, and antioxidant activity during 90 days of storage (11% relative humidity, 4 and 25 ºC). JM and JFM were thermally stable, with satisfactory stability in temperature variations.

Controlling Root Canal Infection in Oncological Patients with Apical Periodontitis.

Introduction: This study evaluated the effectiveness of chemomechanical preparation associated with intracanal medication in reducing the total bacterial load of infected root canals in cancer patients undergoing antineoplastic treatment. The prevalence, levels, and response to treatment of Streptococcus and Actinobacteria species were also assessed.

Methods: The root canals of teeth with apical periodontitis from oncological and control patients were treated using rotary instrumentation, 2.

Incorporation of mixed strawberry and acerola jam into Greek-style goat yogurt with autochthonous adjunct culture of Limosilactobacillus mucosae CNPC007: Impact on technological, nutritional, bioactive, and microbiological properties.

This study aimed to assess the impact of adding strawberry and acerola jam, along with Limosilactobacillus mucosae CNPC007, on the technological, nutritional, bioactive, and microbiological properties of Greek-style goat yogurt. Six yogurt formulations were developed: without and with the addition of L. mucosae CNPC007 (CY and PY, respectively), and with 10 % and 15 % jam (CY10, CY15, PY10, and PY15, respectively).

Effects of atrazine, diuron and glyphosate mixtures on zebrafish embryos: acute toxicity and oxidative stress responses.

Synthetic pesticides are known for their toxic effects on non-target aquatic organisms. However, little is known about their effects when present in mixtures, which are closer to realistic exposure scenarios. Therefore, this study evaluates the toxicity of pesticides such as diuron, atrazine and glyphosate, individually and in combination, in zebrafish embryos, investigating their mechanisms of oxidative stress.

Integrated Vocal Deviation Index (IVDI): A Machine Learning Model to Classifier of the General Grade of Vocal Deviation.

Objective: To develop a multiparametric index based on machine learning (ML) to predict and classify the overall degree of vocal deviation (GG).

Method: The sample consisted of 300 dysphonic and non-dysphonic participants of both sexes. Two speech tasks were sustained vowel [a] and connected speech (counting numbers from 1 to 10).

Organic vs. inorganic nitrates: Metabolic and vascular outcomes in STZ-induced diabetes in mice.

Background: Diabetic animals often display dysregulated nitric oxide (NO) metabolism, contributing to vascular dysfunction. This study evaluates the metabolic and vascular effects of organic nitrate isosorbide mononitrate (ISMN) versus inorganic sodium nitrate (NaNO3) in mice with type 1 diabetes mellitus (T1DM) induced by streptozotocin (STZ).

Experimental Approach: T1DM was induced in male C57Bl6 mice with STZ ip and confirmed by fasting glucose.

Jerked beef as an ultraprocessed convenience food: Desalting strategies to minimize the impact on lipid and protein oxidation.

Jerked beef (JB) is a high-protein convenience food but shows high degree of oxidation owing to its severe ultraprocessing. This study aimed to investigate the effect of desalting processes on oxidative stability of JB. JB were submitted to five desalting procedures: immersion in water at room temperature for 12 h without changing the water (AT12); immersion in water at room temperature for 12 h followed by boiling for 30 min and changing the water between the two procedures (AT12 + C30); immersion in boiled water for 10, 20, and 30 min (C10, C20, and C30, respectively).

Analysis of metabolites associated with ADIPOQ genotypes in individuals with type 2 diabetes mellitus.

Diabetes mellitus (DM) is a significant public health problem and it is known that the identification of molecular markers involved in glycemic control can impact disease control. Although the rs266729 polymorphism located in the promoter of the adiponectin gene (ADP) has been shown to be a candidate for involvement in glycemic control, the genotypic groups have never been characterized in terms of metabolomic aspects. Objective: Analyze the metabolites present in the rs266729 genotype groups.

Can acoustic measurements predict gender perception in the voice?

Purpose: To determine if there is an association between vocal gender presentation and the gender and context of the listener.

Method: Quantitative and transversal study. 47 speakers of Brazilian Portuguese of different genders were recorded.

Food Consumption According to the NOVA Food Classification and Its Relationship with Symptoms of Depression, Anxiety, and Stress in Women.

Background/objectives: Depression, anxiety, and stress are highly prevalent mental disorders worldwide, and food consumption can change in individuals with these conditions. We aimed to assess the food consumption of women with depressive symptoms and compare it to a control without symptoms.

Methods: A cross-sectional study was conducted with 96 women, aged 18-59, allocated into two groups: control = 62) or depressive symptoms ( = 34).

Effects of maternal preconception high-fat diet on the fertility of dams and on the somatic parameters and reflex ontogeny of their male offspring.

Background: Female consumption of a high-fat diet (HFD) may cause fertility issues and affects offspring development.

Objective: Evaluate the acute maternal preconception intake of a HFD on the fertility and reproduction parameters of breeding females; and on the somatic parameters and reflex ontogeny of male offspring.

Methods: Twenty-four rats were randomized into control (PC; n=12) and high-fat diet group (PHF; n=12) that consumed their respective diets during the 23-day preconception period.