Measuring Growth, Resistance, and Recovery after Artemisinin Treatment of Plasmodium falciparum in a single semi-high-throughput Assay.

Background: Artemisinin partial resistance (ART-R) has spread throughout Southeast Asia and mutations in , the molecular marker of resistance, are widely reported in East Africa. Effective assays and robust phenotypes are crucial for monitoring populations for the emergence and spread of resistance. The recently developed extended Recovery Ring-stage Survival Assay used a qPCR-based readout to reduce the labor intensiveness for phenotyping of ART-R and improved correlation with the clinical phenotype of ART-R.

Models of DEIB: Part I - Approaches to Inclusion from Other Health Professions for Consideration by Dentistry.

This article represents a prologue of the discussion of the article "Models of DEIB: Part II-Exploring Models of Inclusion from other Health Professions for Dentistry". It explores existing practices and philosophies from other disciplines that could be applied toward creating environments of inclusion and belonging in dentistry. The primary focus here is to provide an opportunity for the dental profession to leverage knowledge and experiences from other health professions to enhance and expand inclusion efforts and provide enhance engagement at all levels.

Socio-hydrological lock-in; an emergent phenomenon in the face of anthropogenic drought.

This study investigates the drought of three major terminal lakes: Great Salt Lake, Salton Sea, and Lake Urmia, driven by socio-hydrological lock-in-a phenomenon characterized by feedback loops between human activities and environmental processes. Previous research has linked this drying to socio-hydrological lock-in, where rational actions by individuals collectively lead to suboptimal outcomes, exacerbating water scarcity and ecological degradation. Despite existing studies, a critical knowledge gap remains in understanding how these feedback mechanisms operate across different socio-economic and ecological contexts.

Decarboxylation of the Catalytic Lysine Residue by the C5α-Methyl-Substituted Carbapenem NA-1-157 Leads to Potent Inhibition of the OXA-58 Carbapenemase.

Antibiotic resistance in bacteria is a major global health concern. The wide spread of carbapenemases, bacterial enzymes that degrade the last-resort carbapenem antibiotics, is responsible for multidrug resistance in bacterial pathogens and has further significantly exacerbated this problem. is one of the leading nosocomial pathogens due to the acquisition and wide dissemination of carbapenem-hydrolyzing class D β-lactamases, which have dramatically diminished available therapeutic options.

Community-associated methicillin-resistant in the Kimberley region of Western Australia, epidemiology and burden on hospitals.

This study presents surveillance data from 1 July 2003 to 30 June 2023 for community-associated methicillin-resistant (CA-MRSA) notified in the Kimberley region of Western Australia (WA) and describes the region's changing CA-MRSA epidemiology over this period. A subset of CA-MRSA notifications from 1 July 2003 to 30 June 2015 were linked to inpatient and emergency department records. Episodes of care (EOC) during which a positive CA-MRSA specimen was collected within the first 48 hours of admission and emergency presentations (EP) during which a positive CA-MRSA specimen was collected on the same day as presentation were selected and analysed further.

The Existence of an Intra-Amniotic Microbiome: Assessing a Controversy.

The existence of intra-amniotic and placental microbiomes during pregnancy has been the source of considerable debate, with research yielding conflicting evidence. This study evaluated investigations into the putative presence of these microbiomes in healthy pregnancies by identifying design and data interpretation issues, particularly concerning contamination in samples with low-density bacterial DNA. Positive findings from diverse populations suggest a consistent presence of microbiota in the intra-amniotic space.

The J Domain Proteins of , a Zoonotic Malaria Parasite of Humans.

is a zoonotic form of human malaria, the pathology of which is poorly understood. While the J domain protein (JDP) family has been extensively studied in , and shown to contribute to malaria pathology, there is currently very limited information on the JDPs (PkJDPs). This review provides a critical analysis of the literature and publicly available data on PkJDPs.

SOD1 Is an Integral Yet Insufficient Oxidizer of Hydrogen Sulfide in Trisomy 21 B Lymphocytes and Can Be Augmented by a Pleiotropic Carbon Nanozyme.

Down syndrome (DS) is a multisystemic disorder that includes accelerated aging caused by trisomy 21. In particular, overexpression of cystathionine-β-synthase (CBS) is linked to excess intracellular hydrogen sulfide (HS), a mitochondrial toxin at higher concentrations, which impairs cellular viability. Concurrent overexpression of superoxide dismutase 1 (SOD1) may increase oxidative stress by generating excess hydrogen peroxide (HO) while also mitigating the toxic HS burden via a non-canonical sulfide-oxidizing mechanism.

Effect of Co Irradiation on Boehmite Dissolution in Caustic Solutions.

Here, we examine how radiation impacts the dissolution behavior of boehmite by subjecting dry nanoparticles of different sizes to Co γ radiation and subsequently analyzing their dissolution behavior in caustic solutions as a function of temperature. The measured kinetics show that irradiation with an amount 228.24 Mrad significantly slows the dissolution rate, particularly for smaller sizes at lower temperatures.

Designing Phenolphthalein-Based Adsorptive Membranes for the High-Affinity, High-Capacity Capture of Contaminants from Water.

The selective removal of solutes is crucial for ensuring a sustainable water supply, recovering resources, and cost-effective biomanufacturing. Adsorptive membranes are promising in this regard due to their rapid mass transfer and low energy demands. However, state-of-the-art adsorptive membranes offer limited pore sizes and surface chemistries.

Stability and Robustness of Time-discretization Schemes for the Allen-Cahn Equation via Bifurcation and Perturbation Analysis.

The Allen-Cahn equation is a fundamental model for phase transitions, offering critical insights into the dynamics of interface evolution in various physical systems. This paper investigates the stability and robustness of frequently utilized time-discretization numerical schemes for solving the Allen-Cahn equation, with focuses on the Backward Euler, Crank-Nicolson (CN), convex splitting of modified CN, and Diagonally Implicit Runge-Kutta (DIRK) methods. Our stability analysis reveals that the Convex Splitting of the Modified CN scheme exhibits unconditional stability, allowing greater flexibility in time step size selection, while the other schemes are conditionally stable.

How often are ecosystems top-down controlled? Experiments in grassland, grasshopper, and bird systems over time and space.

Ecosystems are frequently considered to be controlled by predation (top-down). Experiments examined this in four bird/spider/grasshopper/prairie habitats over 34 years, employing in each habitat three 100 m bird exclosures and controls (121 habitat/year cases) where plant, grasshopper, and spider abundances were measured. Top-down control (plants decrease and grasshoppers increase with bird exclusion) was observed in only 13.

Cell-to-cell distance that combines gene expression and gene embeddings.

The application of large-language models (LLMs) to single-cell gene-expression data has introduced a new type of data that includes a gene-embedding matrix, in addition to the experimentally obtained gene-expression matrix. This paper addresses a fundamental problem in analyzing such data: how to effectively combine the information from both matrices to better define cell-to-cell distance. We identify a computationally feasible solution that demonstrates superior ability to cluster cells of the same type across all six real datasets we tested, underscoring its advantage as a measure of cell-to-cell distance.

Online health literacy resources for people with intellectual disability: protocol for a grey literature scoping review.

Introduction: People with intellectual disability are at risk of poor physical and mental health. Risks to health are compounded by poor health literacy, that is, reduced capacity to access health services, respond quickly to changes in health status and navigate care pathways. Building health literacy skills is a strength-based way to increase health and optimise the use of healthcare services.

Coordinated Anions Tune Z-Type Ligand Displacement from Colloidal CdSe and InP Nanocrystal Surfaces.

Neutral metal salts coordinate to the surfaces of colloidal semiconductor nanocrystals (NCs) by acting as Lewis acid acceptors for the NC surface anions. This ligand coordination has been associated with increased emission due to the passivation of surface hole traps. Here, variation of the anionic ligands of metal salts is used to study anion effects on metal complex Lewis acidity and surface coordination at CdSe and InP NCs.

MalKinID: A Classification Model for Identifying Malaria Parasite Genealogical Relationships Using Identity-by-Descent.

Pathogen genomics is a powerful tool for tracking infectious disease transmission. In malaria, identity-by-descent (IBD) is used to assess the genetic relatedness between parasites and has been used to study transmission and importation. In theory, IBD can be used to distinguish genealogical relationships to reconstruct transmission history or identify parasites for quantitative-trait-locus experiments.

Search for Soft Unclustered Energy Patterns in Proton-Proton Collisions at 13 TeV.

The first search for soft unclustered energy patterns (SUEPs) is performed using an integrated luminosity of 138  fb^{-1} of proton-proton collision data at sqrt[s]=13  TeV, collected in 2016-2018 by the CMS detector at the LHC. Such SUEPs are predicted by hidden valley models with a new, confining force with a large 't Hooft coupling. In events with boosted topologies, selected by high-threshold hadronic triggers, the multiplicity and sphericity of clustered tracks are used to reject the background from standard model quantum chromodynamics.

Medication Exposure and Mortality in Patients With Schizophrenia.

Importance: The use of antipsychotics, antidepressants, and benzodiazepines may influence the risk of mortality in people with schizophrenia. However, many observational studies have not accounted for immortal time bias (ITB), which occurs when there is a period during which patients in the exposed group are necessarily alive and misclassified as exposed (the period between start of follow-up and initiation of drug). Ignoring ITB may lead to misinterpretation of the association between these drugs and mortality.

Assessing solvated electron uptake in low-temperature plasma-exposed solutions as a pathway to quantifying plasma electrons.

Low-temperature plasma (LTP) is being advanced as an alternative radiation source that offers unique chemical properties demonstrated in a variety of reactive plasma species, in which electrons are the primary species derived upon plasma irradiation. We employed a simple and reliable protocol based on two-electron reduction of nitrate into nitrite ions coupled with fluorometric detection to quantify the uptakes of solvated electrons in irradiated solutions under two distinct plasma conditions of our LTP source. In addition, we explored the influence of other plasma species and the solution pH on the uptake rates.