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Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages.

Stem Cells

September 2005

Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle International Centre for Life, Newcastle NE1 3BZ, United Kingdom.

Louise Hyslop Miodrag Stojkovic Lyle Armstrong Theresia Walter Petra Stojkovic

The homeobox transcription factor Nanog has been proposed to play a crucial role in the maintenance of the undifferentiated state of murine embryonic stem cells. A human counterpart, NANOG, has been identified, but its function and localization have not hitherto been described. We have used a combination of RNA interference and quantitative real-time polymerase chain reaction to study NANOG in human embryonic stem and embryonic carcinoma cells.

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