Farmers' assessment of the social and ecological values of land uses in central highland Ethiopia.

Often in land use evaluations, especially those in developing countries, only the financial aspect receives serious attention, while the social and ecological values are overlooked. This study compared the social and ecological values of four land use types (small-scale woodlot [SSW], boundary tree and shrub planting [BTP], homestead tree and shrub growing [HTG] and cereal farming [CF]) by a criteria-based scoring approach using a bao game. The impacts of local wealth status and proximity to a forest on the value the community renders to the land use types were also assessed.

Reduction of greenhouse gas emissions from feed supply chains by utilizing regionally produced protein sources: the case of Austrian dairy production.

Background: The aim of this study was to analyse the potential greenhouse gas emissions (GHGE) for regionally alternative produced protein-rich feedstuffs (APRFs) which are utilized for dairy cattle in Austria in comparison to solvent-extracted soybean meal (SBME). In addition to GHGE from agriculture and related upstream supply chains, the effects of land use change were calculated and were included in the results for GHGE. Furthermore, mixtures of APRFs were evaluated which provided energy and utilizable protein equivalent to SBME.

A β-glucosidase from Oenococcus oeni ATCC BAA-1163 with potential for aroma release in wine: Cloning and expression in E. coli.

Lactic acid bacteria (LAB) are responsible for olfactory changes in wine during malolactic fermentation (MLF). A side characteristic of MLF is the release of grape derived aroma compounds from their glycosylated precursors by β-glycosidase activities of these bacteria. Apart from Oenococcus oeni, which is regarded as the most promising species for MLF, glycosidic activities have also been observed in wine related members of the genera Lactobacillus and Pediococcus.

Genetic analysis of calf and heifer losses in Danish Holstein.

Mortality in dairy cattle is not only relevant with regard to economic losses but also to animal health and welfare. Thus, the aim of this investigation was to explore the genetic background of postnatal mortality in calves and replacement heifers in different age groups until first calving in Danish Holsteins. Records of Danish Holstein heifer calves born in the years 1998 to 2007 were extracted from the Danish Cattle database (Danish Cattle, Skejby, Denmark).

Adsorption of plasmid DNA on ceramic hydroxyapatite chromatographic materials.

The adsorption of plasmid DNA onto two different types of ceramic hydroxyapatite beads with a particle diameter of 20  μm, namely Ceramic Hydroxyapatite Type II and the Type III, which is not commercially available, were investigated. Type II and the Type III have a pore diameter of 80 and 240  nm, respectively. Equilibrium and dynamic binding capacity for a 4.

Efficient Synthesis of 4-Amino-4-deoxy-L-arabinose and Spacer-equipped 4-Amino-4-deoxy-L-arabinopyranosides by Transglycosylation Reactions.

Methyl 4-azido-4-deoxy-β-L-arabinopyranoside has been synthesized in five steps starting from methyl β-D-xylopyranoside in a multigram scale without chromatographic purification in 78% overall yield. The transformation relied on selective tosylation/nosylation at O-4 followed by acylation, S(N)2 displacement with sodium azide and subsequent deprotection. The methyl 4-azido-4-deoxy-arabinoside was then converted into allyl, propenyl, ω-bromohexyl and chlorethoxyethyl spacer glycosides by transglycosylation with the respective alcohols in good yields and fair anomeric selectivity.

Peptide affinity chromatography media that bind N(pro) fusion proteins under chaotropic conditions.

To design a generic purification platform and to combine the advantages of fusion protein technology and matrix-assisted refolding, a peptide affinity medium was developed that binds inclusion body-derived N(pro) fusion proteins under chaotropic conditions. Proteins were expressed in Escherichia coli using an expression system comprising the autoprotease N(pro) from Pestivirus, or its engineered mutant called EDDIE, with C-terminally linked target proteins. Upon refolding, the autoprotease became active and cleaved off its fusion partner, forming an authentic N-terminus.

Understanding of bonding and mechanical characteristics of cementitious mineral tobermorite from first principles.

This paper reports density functional theory study of the structural and mechanical properties of tobermorite mineral (9 Å phase) as one of the main component of cementitious materials in a concrete chemistry. Calculated bulk modulus and elastic constants reflect a relatively high resistance of the tobermorite structure with respect to external isostatic compression. Moreover, the elastic constants proved the anisotropic character of the tobermorite structure.

Characterization of isoflavone composition in soy-based nutritional supplements via ultra performance liquid chromatography.

The specific isoflavone composition of nutritional supplements is commonly not-labeled, although the stated amounts are strongly dependent on the present isoflavone conjugates. Hence, 11 soy-based dietary supplements were characterized via a newly established ultra performance liquid chromatography (UPLC) method, on both their native conjugated isoflavone spectra, as well as on quantitative amounts derived as total aglycones after enzymatic hydrolysis utilizing Helix pomatia juice. Capitalizing on sub-2 microm particles, the established RP-UPLC technique facilitated efficient chromatographic separation of all 12 soy intrinsic isoflavone forms within 10 min.

Two-dimensional difference fluorescence gel electrophoresis to verify the scale-up of a non-affinity-based downstream process for isolation of a therapeutic recombinant antibody.

For therapeutic antibody production Protein A chromatography is often replaced by non-affinity-based purification sequences, which are considered as more economical. 2-D DIGE was applied for evaluation of scale-up of non-affinity based process of a humanized monoclonal antibody, anti-Rh(D) IgG(1), in comparison with other conventional analytical methods, like SDS-PAGE, Western blot, or SEC. Due to a high sensitivity of this technique (125 pg protein/spot) and high dynamic range of five orders of magnitude, low molecular weight impurities were detected in purified samples.

miR-17-92 cluster: ups and downs in cancer and aging.

The miR-17-92 cluster encoding 6 single mature miRNAs was identified a couple of years ago to contain the first oncogenic miRNAs. Now, one of these 6 miRNAs, miR-19 has been identified as the key responsible for this oncogenic activity. This in turn reduces PTEN levels and in consequence activates the AKT/mTOR pathway that is also prominently involved in modulation of organismal life spans.

Genetic parameters for abnormal sucking traits in Austrian Fleckvieh heifers.

Cross-sucking and intersucking are considered abnormal behaviours in cattle and constitute a common problem in dairy farming. Cross-sucking in calves is defined as sucking any body parts of another calf whereas intersucking in heifers and cows is defined as sucking the udder or udder area. The aim of this study was to determine the genetic variability for abnormal sucking behaviour by estimating genetic parameters and examining individual differences between sires with large progeny groups.

Evaluation of different expression systems for the heterologous expression of pyranose 2-oxidase from Trametes multicolor in E. coli.

The heterologous production of the industrially relevant fungal enzyme pyranose 2-oxidase in the prokaryotic host E. coli was investigated using 3 different expression systems, i.e.

Maximum yields of microsomal-type membranes from small amounts of plant material without requiring ultracentrifugation.

Isolation of a microsomal membrane fraction is a common procedure in studies involving membrane proteins. By conventional definition, microsomal membranes are collected by centrifugation of a postmitochondrial fraction at 100,000g in an ultracentrifuge, a method originally developed for large amounts of mammalian tissue. We present a method for isolating microsomal-type membranes from small amounts of Arabidopsis thaliana plant material that does not rely on ultracentrifugation but instead uses the lower relative centrifugal force (21,000g) of a microcentrifuge.

Potential health-modulating effects of isoflavones and metabolites via activation of PPAR and AhR.

Isoflavones have multiple actions on cell functions. The most prominent one is the activation of estrogen receptors. Other functions are often overlooked, but are equally important and explain the beneficial health effects of isoflavones.

Gaseous nitrogen and carbon emissions from a full-scale deammonification plant.

The aim of this work was to give a quantitative description of the gaseous nitrogen and carbon emissions of a full-scale deammonification plant (DEMON system). Deammonification accounted for the net carbon sequestration of 0.16 g CO2/g NO2-N.

Aerogels from unaltered bacterial cellulose: application of scCO2 drying for the preparation of shaped, ultra-lightweight cellulosic aerogels.

Bacterial cellulose produced by the gram-negative bacterium Gluconacetobacter xylinum was found to be an excellent native starting material for preparing shaped ultra-lightweight cellulose aerogels. The procedure comprises thorough washing and sterilization of the aquogel, quantitative solvent exchange and subsequent drying with supercritical carbon dioxide at 40 degrees C and 100 bar. The average density of the obtained dry cellulose aerogels is only about 8 mg x cm(-3) which is comparable to the most lightweight silica aerogels and distinctly lower than all values for cellulosic aerogels obtained from plant cellulose so far.

Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu-binding sites and antibody properties.

Yeast surface display libraries of human IgG1 Fc regions were prepared in which loop sequences at the C-terminal tip of the CH3 domain were randomized. A high percentage of these library members bound to soluble CD64 and Protein A indicating that the randomization step did not grossly interfere with the overall structure of the displayed Fc. Sorting these libraries by FACS for binders against HER2/neu yielded antigen-specific Fc binders (Fcab; Fc antigen binding) of which one was affinity matured, resulting in Fcab clone H10-03-6 which showed >10-fold improvement in antigen-binding activity versus the parental clone.

Cleavage of zearalenone by Trichosporon mycotoxinivorans to a novel nonestrogenic metabolite.

Zearalenone (ZON) is a potent estrogenic mycotoxin produced by several Fusarium species most frequently on maize and therefore can be found in food and animal feed. Since animal production performance is negatively affected by the presence of ZON, its detoxification in contaminated plant material or by-products of bioethanol production would be advantageous. Microbial biotransformation into nontoxic metabolites is one promising approach.

Novel modulators of senescence, aging, and longevity: Small non-coding RNAs enter the stage.

During the last decade evidence has accumulated that the aging process is driven by limited allocation of energy to somatic maintenance resulting in accumulation of stochastic damage. This damage, affecting molecules, cells, and tissues, is counteracted by genetically programmed repair, the efficiency of which thus importantly determines the life and 'health span' of organisms. Therefore, understanding the regulation of gene expression during cellular and organismal aging as well as upon exposure to various damaging events is important to understand the biology of aging and to positively influence the health span.

Harnessing ecosystem models and multi-criteria decision analysis for the support of forest management.

The decision-making environment in forest management (FM) has changed drastically during the last decades. Forest management planning is facing increasing complexity due to a widening portfolio of forest goods and services, a societal demand for a rational, transparent decision process and rising uncertainties concerning future environmental conditions (e.g.

Construction and development of a new single-column simulated moving bed system on the laboratory scale.

Simulated moving bed (SMB) chromatography combines high productivity and high purities with reduced buffer consumption. We have developed a laboratory scale single column SMB (SC-SMB) unit with all four separation zones in one column. Distributors embedded within the chromatographic medium allow introduction and withdrawal of liquid between the zones.

Matrix-assisted refolding of autoprotease fusion proteins on an ion exchange column.

Refolding of proteins must be performed under very dilute conditions to overcome the competing aggregation reaction, which has a high reaction order. Refolding on a chromatography column partially prevents formation of the intermediate form prone to aggregation. A chromatographic refolding procedure was developed using an autoprotease fusion protein with the mutant EDDIE from the N(pro) autoprotease of pestivirus.

Risk factors for lameness in freestall-housed dairy cows across two breeds, farming systems, and countries.

Lameness poses a considerable problem in modern dairy farming. Several new developments (e.g.

A new ultra-pressure liquid chromatography method for the determination of biogenic amines in cheese.

A fast and reliable ultra-performance liquid chromatography (UPLC) method for the determination of biogenic amines (ethanolamine, methylamine, agmatine, histamine, dimethylamine, ethylamine, octopamine, pyrrolidine, dopamine, isopropylamine, propylamine, tyramine, putrescine, butylamine, cadaverine, tryptamine, 2-phenylethylamine, 3-methylbutylamine, spermidine, spermine) in cheese was established. After pre-column derivatization with 6-aminoquinolyl-N-hydroxy-succinimidyl carbamate (AQC), 20 primary and secondary biogenic amines were separated on an Acquity UPLC column (BEH C(18), 1.7 microm; 2.