Recycling honey bee drone brood for sustainable beekeeping.

Pollination by insects is vital for global agriculture, with honey bees (Apis mellifera L.) being the most important pollinators. Honey bees are exposed to numerous stressors, including disease, pesticides, and inadequate nutrition, resulting in significant colony losses.

Ketamine-Assisted Psychotherapy for Antisocial Personality Disorder: A Case Report.

There are currently no evidence-based treatment guidelines for antisocial personality disorder (ASPD). Instead, treatment typically focuses on comorbid conditions. There is currently no literature documenting cases or research, theoretical or otherwise of using ketamine-assisted therapy or any other psychedelic therapy in cases of ASPD.

Fastening in Rock Mass-Structural Design of Shallow Embedded Anchors in Inhomogeneous Substrate.

Unlike traditional base materials such as concrete or masonry, there are no guidelines for rock as a base material for post-installed anchors. The varying rock properties (e.g.

On the Road to Safety: Examining Children's Cycling Skills and Physical Activity Levels.

Background/objectives: Studies indicate a decline in children's physical activity (PA) levels, active mobility, and psychomotor skills, reflected in poorer cycling abilities. These trends are worrying given the rising prevalence of childhood obesity and sedentary lifestyles. This study assessed cycling skills among primary school children in Lower Austria, comparing self-perceptions with objective assessments, and explores the relationship between cycling skills, PA levels, and school performance, as well as the impact of cycling training on skill development.

The benefits of Lactiplantibacillus plantarum: From immunomodulator to vaccine vector.

Probiotics have been increasingly recognized for positively influencing many aspects of human health. Lactiplantibacillus plantarum (L. plantarum), a non-pathogenic bacterium, previously known as Lactobacillus plantarum, is one of the lactic acid bacteria commonly used in fermentation.

Priority actions for Fusarium head blight resistance in durum wheat: Insights from the wheat initiative.

Fusarium head blight (FHB), mainly caused by Fusarium graminearum and Fusarium culmorum, is a major wheat disease. Significant efforts have been made to improve resistance to FHB in bread wheat (Triticum aestivum), but more work is needed for durum wheat (Triticum turgidum spp. durum).

Multiple environmental tracers combined with a constrained Bayesian isotope mixing model to elucidate nitrate and sulfate contamination in a coastal groundwater system.

Several groundwater quality investigations have been conducted in coastal regions that are commonly exposed to multiple anthropogenic stressors. Nonetheless, such studies remain challenging because they require focused-diagnostic approaches for a comprehensive understanding of groundwater contamination. Therefore, this study integrates a multi-tracer approach to acquire comprehensive information allowing for an improved understanding of the origins of groundwater contamination, the relative contribution of contaminants, and their biogeochemical cycling within a coastal groundwater system.

Correction of aberrant splicing of ELP1 pre-mRNA by kinetin derivatives - A structure activity relationship study.

Familial dysautonomia is a debilitating congenital neurodegenerative disorder with no causative therapy. It is caused by a homozygous mutation in ELP1 gene, resulting in the production of the transcript lacking exon 20. The compounds studied as potential treatments include the clinical candidate kinetin, a plant hormone from the cytokinin family.

Recycling electroplating sludge as an efficient photocatalyst for degradation of ciprofloxacin in aqueous solution.

This study explored the potential of electroplating sludge (ESs) as a novel and effective photocatalyst for the photodegradation of ciprofloxacin in aqueous solutions. The characterization of the ESs was evaluated using sophisticated techniques, such as XRD, SEM, TEM, EDX, FTIR, and BET. ESs-derived photocatalyst materials were found to primarily consist of various metal oxides (Ni-O, Cu-O), which can absorb ultraviolet or visible light.

Surface-enhanced Raman spectroscopy as effective tool for detection of sialic acid as cancer biomarker.

Sialic acid, a negatively charged nine-carbon monosaccharide, is mainly located at the terminal end of glycan chains on glycoproteins and glycolipids of cell surface and most secreted proteins. Elevated levels of sialylated glycans have been known as a hallmark in numerous cancers. As a result, sialic acid acts as a useful and accessible cancer biomarker for early cancer detection and monitoring the disease development during cancer treatment which is crucial in elevating the survival rate.

Hemodynamic cortical ripples through cyclicity analysis.

A fine-grained understanding of dynamics in cortical networks is crucial to unpacking brain function. Resting-state functional magnetic resonance imaging (fMRI) gives rise to time series recordings of the activity of different brain regions, which are aperiodic and lack a base frequency. Cyclicity analysis, a novel technique robust under time reparametrizations, is effective in recovering the temporal ordering of such time series, collectively considered components of a multidimensional trajectory.

Improved methodology for tracing a pulse of 13C-labelled tree photosynthate carbon to ectomycorrhizal roots, other soil biota and soil processes in the field.

Isotopic pulse-labelling of photosynthate allows tracing of carbon (C) from tree canopies to belowground biota and calculations of its turnover in roots and recipient soil microorganisms. A high concentration of label is desirable, but is difficult to achieve in field studies of intact ecosystem patches with trees. Moreover, root systems of trees overlap considerably in most forests, which requires a large labelled area to minimize the impact of C allocated belowground by un-labelled trees.

Production of Hyaluronidase by .

Hyaluronidases have been a subject of great interest in medical and cosmeceutical applications. Previously, our group demonstrated that the venom glands of contain hyaluronidase enzymes (VesT2s), and heterologous expression of the corresponding gene () in systems results in inclusion bodies, necessitating functional folding using urea. Here, we report the successful heterologous expression of VesT2a in the expression system, with gene construction achieved using Golden.

Lignin Polyurethane Aerogels: Influence of Solvent on Textural Properties.

This study explores the innovative potential of native lignin as a sustainable biopolyol for synthesizing polyurethane aerogels with variable microstructures, significant specific surface areas, and high mechanical stability. Three types of lignin-Organosolv, Aquasolv, and Soda lignin-were evaluated based on structural characteristics, Klason lignin content, and particle size, with Organosolv lignin being identified as the optimal candidate. The microstructure of lignin polyurethane samples was adjustable by solvent choice: Gelation in DMSO and pyridine, with high affinity to lignin, resulted in dense materials with low specific surface areas, while the use of the low-affinity solvent e.

Integrating In Silico and In Vitro Tools for Optimized Antibody Development-Design of Therapeutic Anti-oxMIF Antibodies.

Background: Rigorous assessment of antibody developability is crucial for optimizing lead candidates before progressing to clinical studies. Recent advances in predictive tools for protein structures, surface properties, stability, and immunogenicity have streamlined the development of new biologics. However, accurate prediction of the impact of single amino acid substitutions on antibody structures remains challenging, due to the diversity of complementarity-determining regions (CDRs), particularly CDR3s.

Development of a luminex-based assay for the detection of anti-capripoxvirus and rift valley fever virus antibodies in domestic ruminants.

The three members of the genus capripoxvirus (CaPV), lumpy skin disease virus (LSDV), sheeppox virus (SPPV), and goatpox virus (GTPV) have common hosts and areas of overlapping geographical distribution with Rift Valley fever virus (RVFV). Hence, to ensure more cost-effective disease surveillance we developed and evaluated a Luminex assay for the simultaneous detection of antibodies against CaPV and RVFV in domestic ruminants. In cattle, the assay had a sensitivity (Se) of 98.

Genetic diversity and distinction of and in traditional Montenegrin brine cheeses and salamis.

Introduction: is a widespread acid-lactic bacterium found in the environment, humans, and animal microbiota, and it also plays a role in the production of traditional food. However, the worldwide emergence of multidrug-resistant strains represents a major public health threat and is the primary reason that the genus is not recommended for the Qualified Presumption of Safety (QPS) list of the European Food Safety Authority (EFSA), raising concerns about its presence in food products.

Methods: In this study, 39 and 5 isolates were obtained from artisanal brine cheeses and dry sausages, sourced from 21 different Montenegrin producers.

From extraction to end-uses and waste management: Modeling economy-wide material cycles and stock dynamics around the world.

Material stocks of infrastructure, buildings, and machinery are the biophysical basis of production and consumption. They are a crucial lever for resource efficiency and a sustainable circular economy. While material stock research has proliferated over the last years, most studies investigated specific materials or end-uses, usually not embedded into an economy-wide perspective.

In-use dissipation of technology-critical elements from vehicles and renewable energy technologies in Vienna, Austria: A public health matter?

The rollout of electric vehicles and photovoltaic panels is essential to mitigate climate change. However, they depend on technology-critical elements (TCEs), which can be harmful to human health and whose use is rapidly expanding, while recycling is lacking. While mining has received substantial attention, in-use dissipation in urban areas has so far not been assessed, for example, corrosion and abrasion of vehicle components and weather-related effects affecting thin-film photovoltaic panels.

: an important genus of canker pathogens.

species have commonly been reported as important plant pathogenic fungi with wide host ranges and geographic distributions. With the increase in the number of cryptic species being described, a comprehensive global taxonomic revision of the genus is required. The present study includes 399 isolates from 32 countries.

The memory of past water abundance shapes trees 7 years later.

Premise: Tree structure and function are constrained by and acclimate to climatic conditions. Drought limits plant growth and carbon acquisition and can result in "legacy" effects that last beyond the period of water stress. Leaf and twig-level legacy effects of past water abundance, such as that experienced by trees that established under wetter conditions are unknown.

Understanding Uncertainty in Market-Mediated Responses to US Oilseed Biodiesel Demand: Sensitivity of ILUC Emission Estimates to GLOBIOM Parametric Uncertainty.

The life cycle greenhouse gas (GHG) emissions of biofuels depend on uncertain estimates of induced land use change (ILUC) and subsequent emissions from carbon stock changes. Demand for oilseed-based biofuels is associated with particularly complex market and supply chain dynamics, which must be considered. Using the global partial equilibrium model GLOBIOM, this study explores the uncertainty in market-mediated impacts and ILUC-related emissions from increasing demand for soybean biodiesel in the United States in the period 2020-2050.

Engineering for production of active cannabinoid synthases via secretory pathway optimization.

The production of cannabinoid compounds such as Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabichromene (CBC) with potential pharmaceutical applications is growing sharply. However, challenges such as the low yield of minor cannabinoids, legal restrictions on cultivation, and the complexity and cost of purification from the Cannabis sativa plant necessitate a biotechnological approach. Since the biosynthetic pathway is disclosed, cannabinoids have been produced in yeast, insect cells and plants mainly by the heterologous expression of tetrahydrocannabinol acid synthase (THCAS).

Genetic Diversity, Chemical Constituents, and Bioactivities of from Thailand.

The genus (Capparaceae) comprises 85 woody species distributed across the paleotropics, with some species used in traditional medicine. This study investigated the phylogenetic placement, genetic diversity, and phytochemical composition of , a species native to Indochina. Phylogenetic analyses using and markers confirmed the monophyly of the specimens collected from Thailand.