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Overexpression of Candida albicans secretory aspartyl proteinase 2 and its expression in Saccharomyces cerevisiae do not augment virulence in mice.
Microbiology (Reading)
August 1998
Department of Microbiology and Immunology, Faculty of Medicine, University of Montreal and Sainte-Justine Hospital3175 CoCte Sainte-Catherine, Montreal, Québec H3T 1C5Canada.
To elucidate the implications of secreted aspartyl proteinase (Sap)2p in the pathogenesis of Candida infections, the SAP2 gene was expressed in Saccharomyces cerevisiae and overexpressed in Candida albicans. The coding region of SAP2, including its signal sequence and propeptide, was amplified by PCR and cloned downstream of the S. cerevisiae or C.
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