5 results match your criteria: "University of Minnesota Cancer Center and Department of Medicinal Chemistry[Affiliation]"
Sequence distribution of acetaldehyde-derived N2-ethyl-dG adducts along duplex DNA.
Chem Res Toxicol
October 2007
University of Minnesota Cancer Center and Department of Medicinal Chemistry, Minneapolis 55455, USA.
Acetaldehyde (AA) is the major metabolite of ethanol and may be responsible for an increased gastrointestinal cancer risk associated with alcohol beverage consumption. Furthermore, AA is one of the most abundant carcinogens in tobacco smoke and induces tumors of the respiratory tract in laboratory animals. AA binding to DNA induces Schiff base adducts at the exocyclic amino group of dG, N2-ethylidene-dG, which are reversible on the nucleoside level but can be stabilized by reduction to N2-ethyl-dG.
View Article and Find Full Text PDFStructural elucidation of a novel DNA-DNA cross-link of 1,2,3,4-diepoxybutane.
Chem Res Toxicol
February 2007
University of Minnesota Cancer Center and Department of Medicinal Chemistry, Minneapolis, Minnesota 55455, USA.
Article Synopsis
- DNA-DNA cross-linking by 1,2,3,4-diepoxybutane (DEB) leads to the formation of harmful DNA lesions, initiating its cytotoxic and genotoxic effects.
- Researchers identified a new bifunctional DNA lesion called N1HX-N7G-BD, created when DEB interacts with guanine and adenine, and developed a standard for further analysis.
- The presence of N1HX-N7G-BD in DEB-treated DNA was confirmed through various analytical techniques, suggesting it could contribute to mutations even though it is less common than other types of DNA damage.
Cross-linking of the human DNA repair protein O6-alkylguanine DNA alkyltransferase to DNA in the presence of 1,2,3,4-diepoxybutane.
Chem Res Toxicol
May 2006
University of Minnesota Cancer Center and Department of Medicinal Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.
1,2,3,4-Diepoxybutane (DEB) is a key carcinogenic metabolite of the important industrial chemical 1,3-butadiene. DEB is a bifunctional alkylating agent capable of reacting with DNA and proteins. Initial DNA alkylation by DEB produces N7-(2'-hydroxy-3',4'-epoxybut-1'-yl)-guanine monoadducts, which can react with another nucleophilic site to form cross-linked adducts.
View Article and Find Full Text PDFStructural characterization of the major DNA-DNA cross-link of 1,2,3,4-diepoxybutane.
Chem Res Toxicol
February 2004
University of Minnesota Cancer Center and Department of Medicinal Chemistry, 806 Mayo, 420 Delaware Street Southeast, Minneapolis, Minnesota 55455, USA.
1,2,3,4-Diepoxybutane (DEB) is a bifunctional alkylating agent that exhibits both cytotoxic and promutagenic properties. DEB is the ultimate carcinogenic species of the major industrial chemical 1,3-butadiene (BD), as well as the active form of the antitumor prodrug treosulfan. DEB is tumorigenic in laboratory animals and is capable of inducing a variety of genotoxic outcomes, including point mutations, large deletions, and chromosomal aberrations.
View Article and Find Full Text PDFLocating nucleobase lesions within DNA sequences by MALDI-TOF mass spectral analysis of exonuclease ladders.
Chem Res Toxicol
August 2001
University of Minnesota Cancer Center and Department of Medicinal Chemistry, Minneapolis, MN 55455, USA.
The location of carcinogen-modified nucleobases (DNA adducts) within DNA sequences is a critical factor affecting their promutagenic properties and persistence in DNA. We now report the use of controlled exonuclease digestion followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to directly map modified nucleobases within DNA. The DNA sequence is determined by mass spectral analysis of the DNA ladders produced by sequential removal of nucleotides with either 5'-->3' or 3'-->5' exonuclease.
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