1 results match your criteria: "University of Maryland School of Medicine (UMGCCC)[Affiliation]"
Lipid tethering of breast tumor cells reduces cell aggregation during mammosphere formation.
Sci Rep
February 2021
Marlene and Stewart Greenebaum Comprehensive Cancer Center, University of Maryland School of Medicine (UMGCCC), 22 S. Greene St., Baltimore, MD, 21201, USA.
Article Synopsis
- Mammosphere assays help identify cancer-initiating stem cells that can form spheres in a lab setting, but traditional methods lead to cell clumping, making it hard to measure true efficiency.
- A new technique using lipid anchors was developed to reduce cell aggregation while allowing for free-floating growth, improving the accuracy of monitoring mammosphere formation.
- This method resulted in a significantly higher percentage of clonal mammospheres and better size correlation compared to traditional low-attachment approaches, indicating more reliable assay outcomes.