Whole genome analysis revealed the role of and genes in carbapenem resistance of strains.

is a multidrug-resistant bacterium that has emerged as a significant nosocomial pathogen globally and renowned for its ability to acquire antimicrobial resistance (AMR) genes. However, understanding of its resistance mechanisms to certain drug classes remains limited. This study focused on four bacterial strains (AB863, AB889, AB930, and AB960) exhibiting carbapenem resistance.

Unravelling the Significance of Seed Proteomics: Insights into Seed Development, Function, and Agricultural Applications.

Seeds are essential for plant reproduction, ensuring species survival and dispersal while adapting to diverse environments throughout a plant's life. Proteomics has emerged as a powerful tool for deciphering the complexities of seed growth, germination, and stress responses. Advanced proteomic technologies enable the analysis of protein changes during germination, dormancy, and ageing, enhancing our understanding of seed lifespan and vitality.

Reaching machine learning leverage to advance performance of electrocatalytic CO conversion in non-aqueous deep eutectic electrolytes.

Deep eutectic electrolytes (DEEs) show promise for future electrochemical systems due to their adjustable buffer capacities. This study utilizes machine learning algorithms to analyse the carbon dioxide reduction reaction (CORR) in DEEs with a buffer capacity of approximately 10.21 mol/pH.

Extraction of Pyrrole from Its Mixture with -Hexadecane Using Protic Ionic Liquids.

The removal of nitrogen compounds from fuel via the conventional method, which is hydrodenitrogenation, is costly and involves catalysts and energy-intensive conditions (600 K and 300 atm). Recently, ionic liquids (ILs) have emerged as a promising alternative solvent for the denitrogenation of fuel oil. However, certain ILs are expensive and challenging to synthesize, prompting the exploration of protic ionic liquid (PIL) substitutes, which offer similar advantages to ILs.

Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats.

Hypertension is a risk factor for cardiovascular diseases such as coronary artery disease, heart failure, and stroke. Lignosus rhinocerus (Cooke) Ryvarden (also known as tiger milk mushroom), has been reported to exhibit a range of pharmacological effects, such as anti-inflammatory, anti-proliferative, antioxidative, immunomodulatory and anti-asthmatic activities. Thus far, there is limited research that has explored its ability to mediate vascular effects in vivo.

Expansion of Smoke-Free Laws in Public Places and Support for Smoke-Free in Malaysia: Findings from the 2020 ITC Malaysia Survey.

Smoke-free laws (SFL) are more effective with public support. This study investigated the smoking prevalence, public perceptions of smoking rules, and support for comprehensive SFL among 1047 people who smoke (PWS) and 206 people who do not smoke (PNS) aged ≥18 in the 2020 International Tobacco Control Malaysia Survey. Smoking prevalence was highest in nighttime entertainment venues (85.

A review on reactive oxygen species-induced mechanism pathways of pharmaceutical waste degradation: Acetaminophen as a drug waste model.

Innately designed to induce physiological changes, pharmaceuticals are foreknowingly hazardous to the ecosystem. Advanced oxidation processes (AOPs) are recognized as a set of contemporary and highly efficient methods being used as a contrivance for the removal of pharmaceutical residues. Since reactive oxygen species (ROS) are formed in these processes to interact and contribute directly toward the oxidation of target contaminant(s), a profound insight regarding the mechanisms of ROS leading to the degradation of pharmaceuticals is fundamentally significant.

Poly-(ionic liquid) coated with magnetic nanoparticles for micro solid phase extraction of polycyclic aromatic hydrocarbons in food samples.

Poly(methyl methacrylate-vinyl imidazole bromide) (poly-MMA-IL)-grafted magnetic nanoparticles were successfully developed and applied in the micro-magnetic solid phase extraction (μ-MSPE) for 16 types of polycyclic aromatic hydrocarbons (PAHs) from tea, fried food, and grilled food samples via gas chromatography flame ionization detector (GC-FID). One variable at a time (OVAT) and response surface methodology (RSM) were used for efficient optimization. The validation method showed a good coefficient of determination () ranging from 0.

An enantioselective study of β-cyclodextrin and ionic liquid-β-cyclodextrin towards propranolol enantiomers by molecular dynamic simulations.

In this study, the enantioselectivity of β-cyclodextrin and its derivatives towards propranolol enantiomers are investigated by molecular dynamic (MD) simulations. β-cyclodextrin (β-CD) have previously been shown to be able to recognize propranolol (PRP) enantiomers. To improve upon the enantioselectivity of β-cyclodextrin, we propose the use of an ionic-liquid-modified-β-cyclodextrin (β-CD-IL).

COSMO-RS-based assessment of thermodynamic tools in predicting the polar and non-polar solvents efficiency in vegetable oil extraction.

Context: One of the prevalent methods for evaluating separation performance is to predict the interactions of solvent and solute molecules. The infinite dilution activity coefficient, Gibbs free energy, and sigma profiles provided insights into the solubilization of a solute and revealed the intensity of the solution's molecular interactions. The effective thermodynamic tools (infinite dilution activity coefficient, Gibbs free energy) were evaluated for predicting the efficiency of 18 polar and non-polar organic solvents in rubber seed oil (RSO) extraction.

A kinetic study of ex-situ soil remediation by nickel extraction using natural deep eutectic solvent.

Using natural deep eutectic solvents (NADESs) as a green reagent is a step toward producing environmentally friendly and sustainable technology. This study screened three natural DESs developed using quaternary ammonium salt and organic acid to analyse their capability to extract nickel ions from contaminated mangrove soil, which are ChCl: Acetic Acid (ChCl-AceA), ChCl: Levulinic Acid (ChCl-LevA), and ChCl: Ethylene Glycol(ChCl-Eg) at molar ratio 1:2. The impact of various operating parameters such as washing agent concentration, pH solution, and contact time on the NADES performance in the dissolution of Ni ions batch experiments were performed.

AuNRs attached TiO NPs modified by cobalt-imidazolate frameworks as exceptional materials to improve the energy conversion efficiency of dye-sensitized solar cells.

This work reports a significant improvement in both the open-circuit voltage () and current density () of dye-sensitized solar cells (DSSCs) using gold nanorod-modified TiO nanoparticles (TiO/AuNRs) together with a cobalt-imidazolate framework (ZIF-67) as an efficient photoanode. It was demonstrated that adding ZIF-67 (8 wt%) to TiO NPs increased the by 160 mV and by 2.5 times.

Changes in the Proteome Profile of A549 Cells Following Helichrysetin-Induced Apoptosis Suggest the Involvement of DNA Damage Response and Cell Cycle Arrest-Associated Proteins.

Our previous findings demonstrated that Helichrysetin possessed promising anti-cancer activity. It was able to induce apoptosis in the A549 cell line. However, its mechanism of action is unknown.

Palm Raceme as a Promising Biomass Precursor for Activated Carbon to Promote Lipase Activity with the Aid of Eutectic Solvents.

This study concerns the role of activated carbon (AC) from palm raceme as a support material for the enhancement of lipase-catalyzed reactions in an aqueous solution, with deep eutectic solvent (DES) as a co-solvent. The effects of carbonization temperature, impregnation ratio, and carbonization time on lipase activity were studied. The activities of Amano lipase from (AML) and lipase from the porcine pancreas (PPL) were used to investigate the optimum conditions for AC preparation.

Poly-(MMA-IL) filter paper: A new class of paper-based analytical device for thin-film microextraction of multi-class antibiotics in environmental water samples using LC-MS/MS analysis.

A paper-based polymeric ionic liquid (p-Poly-(MMA-IL)) was successfully developed by grafting the polymeric ionic liquid on the surface of commercial filter paper (FP) by using the dipping method, presenting a new cost-effective film. The newly developed p-Poly-(MMA-IL) FP was then applied as a paper-based thin-film microextraction (p-TFME) analytical device to extract 14 compounds as representative of five groups of antibiotic drugs, which were sulfonamides, tetracyclines, fluoroquinolones, penicillin and macrolides in environmental water samples. Besides, p-Poly-(MMA-IL) FP, p-Poly-(MMA) FP, and unmodified filter paper were successfully characterised by FTIR, NMR, FESEM, TGA, and XRD techniques.

In vivo anti-tumor activity of Lignosus rhinocerus TM02® using a MCF7-xenograft NCr nude mice model.

Ethnopharmacological Relevance: Lignosus rhinocerus (Cooke) Ryvarden (also known as Tiger Milk mushroom, TMM), is a basidiomycete belonging to the Polyporaceae family. It has been documented to be used by traditional Chinese physicians and indigenous people in Southeast Asia to treat a variety of illnesses, such as gastritis, arthritis, and respiratory conditions, as well as to restore patients' physical well-being. TMM has also been used in folk medicine to treat cancer.

Medicinal Mushrooms and Their Use to Strengthen Respiratory Health during and Post-COVID-19 Pandemic.

COVID-19 infection has been a key threat to the public health system globally, with an estimated 248 million cases worldwide. COVID-19 patients are subject to a higher risk of developing chronic respiratory disorders that are closely associated with long-term disability, multi-morbidity, and premature mortality. Although there have been recent advancements in respiratory treatment regimens, there has also been increased interest in the use of medicinal mushrooms in bridging the unaddressed pathways of action within the treatment algorithms.

Complete chloroplast genome of and a comparative analysis with members of the family Zingiberaceae.

Unlabelled: (L.) Mansf. is a medically important ginger species of the family Zingiberaceae but its genomic information on molecular phylogeny and identification is scarce.

Bifunctional Ionic Deep Eutectic Electrolytes for CO Electroreduction.

CO is a low-cost monomer capable of promoting industrially scalable carboxylation reactions. Sustainable activation of CO through electroreduction process (ECOR) can be achieved in stable electrolyte media. This study synthesized and characterized novel diethyl ammonium chloride-diethanolamine bifunctional ionic deep eutectic electrolyte (DEACl-DEA), using diethanolamine (DEA) as hydrogen bond donors (HBD) and diethyl ammonium chloride (DEACl) as hydrogen bond acceptors (HBA).

Psychometric analysis and reliability of the dental treatment motivation scale for Indonesian pregnant women.

This study aimed to obtain the results of psychometric analysis and reliability of the dental treatment motivation scale (DTMS), which was adapted cross-culturally for pregnant women in Indonesia. A descriptive survey was conducted with 149 pregnant women at a maternal clinic in Bandung City, Indonesia, in December 2020. Convenience sampling was chosen as the sampling technique after the inclusion and exclusion criteria had been fulfilled.

In Vitro Inhibition of Melanin Formation and Enhancement of Collagen Production by a Mushroom Sclerotial Water Extract from the Tiger Milk Mushroom, Lignosus rhinocerus (Agaricomycetes), with No Skin and Eye Irritation.

Lignosus rhinocerus has bioactive properties, such as anti-inflammatory, antioxidant, antiglycation, and immunomodulatory effects, that are beneficial to the skin. Gas chromatography-mass spectrometry analysis revealed the presence of cosmeceutical compounds, including linoleates, ascorbic acid dipalmitate, and all-trans-squalene, in the mushroom sclerotium. We further investigated the depigmentation and antiaging activities of L.

Electroreduction of CO and Quantification in New Transition-Metal-Based Deep Eutectic Solvents Using Single-Atom Ag Electrocatalyst.

Deep eutectic solvents (DESs) are efficient media for CO capture, and an electroreduction process using the deterministic surface of single-atom electrocatalysts is a facile way to screen gas absorption capacities of novel DESs. Using newly prepared transition-metal-based DESs indexed as TDESs, the interfacial mechanism, detection, quantification, and coordination modes of CO were determined for the first time. The CO has a minimum detection time of 300 s, whereas 500 s of continous ambient CO saturation provided ZnCl/ethanolamine (EA) (1:4) and CoCl/EA (1:4) TDESs with a maximum CO absorption capacity of 0.

Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycota), Cold-Water Extract Relaxes the Rat Isolated Bladder.

Ophiocordyceps sinensis is a popular medicinal mushroom used for various health conditions, including alleviation of frequent urination, which is a major symptom of overactive bladder (OAB) syndrome. This study aimed to investigate the effect of O. sinensis (OCS02 cultivar) cold-water extract (CWE) against bladder contractility using the organ bath technique.

Reasons for using e-cigarettes and support for e-cigarette regulations: Findings from the 2020 ITC Malaysia Survey.

Introduction: Malaysia has the largest e-cigarette (EC) market in Southeast Asia, and it has been estimated that 17% of adult daily cigarette smokers also used ECs on a daily basis in 2020. However, few studies have examined the reasons people use ECs in Malaysia. This cross-sectional study of adult cigarette smokers from Malaysia assessed reasons for EC use and their support for key proposed EC regulations.