Antimicrobial resistance genetic determinants and susceptibility profile of Pseudomonas aeruginosa isolated from clinical samples in a tertiary hospital in Ogun State, Nigeria.

Background: Genetic determinants are known to promote antibiotic resistance through horizontal gene transfer.

Methods: We molecularly characterized integrons, plasmid replicon types and metallo-β-lactamase-encoding genes of 38 Pseudomonas aeruginosa strains isolated from clinical samples.

Results: The P.

Evaluation of genetic variability for major agro-morphological and stalk sugar traits in African sorghum genotypes.

The study aimed to assess one hundred and twelve (112) accessions of sorghum sourced from Nigeria and other four African countries for their genetic variability, heritability (broad sense) and genetic advance components to identify promising accessions with high grain yield and sweet stalk which can serve as future parents for breeding dual-purpose lines. The accessions were evaluated at Ilora, Oyo State, Nigeria in a randomized complete block design (RCBD) layout with three replicates in two planting seasons (2020 and 2021). The results showed that the phenotypic coefficient of variation (PCV) was higher than the genotypic coefficient of variation (GCV).

Estimation of radiological impact of the activities of Olusosun Dump Site on workers and dwellers of Olusosun, in Lagos Southwest Nigeria.

This study assessed the potential radiological risks associated with the activities on Olusosun dump site on workers and dwellers of Olusosun community. The activity concentrations of 238U, 232Th and 40K from of soil and water samples were determined using High-Purity Germanium (HPGe) detector. The background radiation level of Olusosun dump site was measured using a portable Geiger-Müller counter-Radeye B20 survey meter.

Gendered share of housework and the COVID-19 pandemic: Examining self-ratings and speculation of others in Germany, India, Nigeria, and South Africa.

This cross-sectional study examined gender differences between male- and female-typed housework during the early COVID-19 lockdowns in 2020. Participants in Germany, India, Nigeria, and South Africa ( = 823) rated their housework share before and during the lockdown, then speculated about the division of housework performed by men and women in general, before and post-lockdown. Women spent more time on female-typed tasks and men (in Nigeria and South Africa) on male-typed tasks before and during the lockdown.

Time-dependent multivariate and spectroscopic characterisation of oil residue in Niger Delta soil.

In this paper, we present a detailed evaluation of changes in the oil residue in soil following a spill using weathering indices obtained from analytical instruments such as UV, IR, GC, and H NMR, and chemometrics based on the time of spill in the Niger Delta region of Nigeria. UV, IR and H NMR spectra of eight (8) oil residue samples were analyzed. The PCA of the UV and IR spectrometric index showed that the first two PCs accounted for 87 and 71% of the variance of the index, respectively.

Molecular and phenotypic characterization of efflux pump and biofilm in multi-drug resistant non-typhoidal Serovars isolated from food animals and handlers in Lagos Nigeria.

Background: Multidrug resistance efflux pumps and biofilm formation are mechanisms by which bacteria can evade the actions of many antimicrobials. Antibiotic resistant non-typhoidal serovars have become wide spread causing infections that result in high morbidity and mortality globally. The aim of this study was to evaluate the efflux pump activity and biofilm forming capability of multidrug resistant non-typhoidal (NTS) serovars isolated from food handlers and animals (cattle, chicken and sheep) in Lagos.

Evaluation of efflux pump activity and biofilm formation in multidrug resistant clinical isolates of Pseudomonas aeruginosa isolated from a Federal Medical Center in Nigeria.

Background: Pseudomonas aeruginosa an opportunistic pathogen, is widely associated with nosocomial infections and exhibits resistance to multiple classes of antibiotics. The aim of this study was to determine the antibiotic resistance profile, biofilm formation and efflux pump activity of Pseudomonas strains isolated from clinical samples in Abeokuta Ogun state Nigeria.

Methods: Fifty suspected Pseudomonas isolates were characterized by standard biochemical tests and PCR using Pseudomonas species -specific primers.

Plasmid profile and role in virulence of salmonella enterica serovars isolated from food animals and humans in Lagos Nigeria.

Infections caused by non-typhoidal (NTS) are common around the world, with high morbidity and mortality rates recorded annually. serovars harbor plasmids of various sizes which may play roles in antibiotic resistance and virulence. The aim of this study was to profile and determine the role of plasmids in ciprofloxacin resistance and virulence of serovars.

Antibiotic Susceptibility Profiles of Isolated from Poultry Farms in Lagos Nigeria - A Pilot Study.

and are among the leading causes of gastroenteritis in humans worldwide, particularly in Africa. Poultry remains a major source of species and a vector of transmission to humans. This pilot study was aimed at isolating and determining the antibiotic susceptibility profiles of spp.

Temperature-dependent extraction kinetics of hydrolyzed collagen from scales of croaker fish using thermal extraction.

This study investigates the kinetics of hydrolyzed collagen extraction from the scales of the croaker fish () at temperature ranging from 60°C to 90°C. Extraction was carried out using hydrothermal treatment over a period of 8 hr, during which the mass of hydrolyzed collagen extracted was obtained every hour. The rate order of extraction was temperature-dependent within the times investigated.

Assessment of occupational exposure to polycyclic aromatic hydrocarbons via involuntary ingestion of soil from contaminated soils in Lagos, Nigeria.

Soils from 12 sites in Lagos area, Nigeria impacted by anthropogenic activities were extracted by ultrasonication and analysed for the concentration of 16 priority polycyclic aromatic hydrocarbons (PAHs) by gas chromatograph-mass spectrometer (GC-MS). The concentration of the sum of PAHs ranged from 0.2 to 254 μg/g at these sites.

Combination of culture-independent and culture-dependent molecular methods for the determination of bacterial community of iru, a fermented Parkia biglobosa seeds.

In this study, bacterial composition of iru produced by natural, uncontrolled fermentation of Parkia biglobosa seeds was assessed using culture-independent method in combination with culture-based genotypic typing techniques. PCR-denaturing gradient gel electrophoresis (DGGE) revealed similarity in DNA fragments with the two DNA extraction methods used and confirmed bacterial diversity in the 16 iru samples from different production regions. DNA sequencing of the highly variable V3 region of the 16S rRNA genes obtained from PCR-DGGE identified species related to Bacillus subtilis as consistent bacterial species in the fermented samples, while other major bands were identified as close relatives of Staphylococcus vitulinus, Morganella morganii, B.

Multivariate analysis of potentially toxic metals in sediments of a tropical coastal lagoon.

Surface sediments collected from the Lagos Lagoon, Nigeria, and three adjoining rivers were analysed for their physicochemical properties and pseudo-total concentration of the potentially toxic metals (PTM) Cd, Cr, Cu, Pb and Zn. The concentration of the PTM varied seasonally and spatially. Odo-Iyaalaro was observed to be the most polluted river, with highest concentrations of 42.

Comparison of three sequential extraction protocols for the fractionation of potentially toxic metals in coastal sediments.

In the determination of the best sequential extraction procedures (SEP) for the speciation of metals in sediment samples from the Lagos lagoon system, three sequential extraction procedures were compared for the fractionation of Cd, Cr, Cu, Pb, and Zn. The SEP compared included a modified Tessier's procedure carried out in five steps, while the two other procedures were the three-step original Community Bureau of Reference (BCR) and the modified BCR techniques (four steps). Quantification of the metal concentration was achieved with a flame atomic absorption spectrophotometer.