Men with learning disabilities who have sex with men in public places: mapping the needs of services and users in south east London.

This survey investigated the prevalence of men with learning disabilities who have sex with men in public places in three south east London boroughs. The work was administered through contact with providers of services for people with learning disabilities and was commissioned by the local health authority. It represents the first example of needs assessment work on this theme.

Thermostable chaperonin from Clostridium thermocellum.

Homologues of the chaperonins Cpn60 and Cpn10 have been purified from the Gram-positive cellulolytic thermophile Clostridium thermocellum. The Cpn60 protein was purified by ATP-affinity chromatography and the Cpn10 protein was purified by gel-filtration, ion-exchange and hydrophobic interaction chromatographies. The identities of the proteins were confirmed by N-terminal sequence analysis and antigenic cross-reactivity.

p70 S6 kinase: an enigma with variations.

The S6 ribosomal protein is phosphorylated by p70 S6 kinase (p70S6k). Although the cellular role of S6 phosphorylation is still not fully clear, studies on p70S6k and its activation have revealed the existence of a novel signalling pathway, clues to the mechanism of action of certain immunosuppressants and insights into the control of gene expression at the levels of transcription and translation.

Summer holiday respite provision for the families of children and young people with learning disabilities.

While the provision of respite care is a relatively recent development in the provision of services to families, it is an area of considerable growth and apparent importance. Most service development and research has focused on the provision of residential or family-based respite care though some studies have commented on the apparent demand for other kinds of relief. This study describes a pilot summer playscheme for children with learning disabilities and the reactions of mothers to its provision.

pH-dependence of the dithiol-oxidizing activity of DsbA (a periplasmic protein thiol:disulphide oxidoreductase) and protein disulphide-isomerase: studies with a novel simple peptide substrate.

A decapeptide containing two cysteine residues at positions 3 and 8 has been designed for use in monitoring the disulphide bond-forming activity of thiol:disulphide oxidoreductases. The peptide contains a tryptophan residue adjacent to one of the cysteine residues and an arginine residue adjacent to the other. Oxidation of this dithiol peptide to the disulphide state is accompanied by a significant change in tryptophan fluorescence emission intensity.

Drivers' biased perceptions of the adverse consequences of drink-driving.

The decision to drink and drive may involve the subjective process of weighing the perceived risks of adverse consequences of the behaviour against perceived utility components. Deterrence theory proposes that an individual will refrain from drink-driving if the perceived chances of experiencing negative outcomes associated with the behaviour are high. Previous research has demonstrated that there may be a mechanism of judgmental bias which influences individuals' perceived probabilities of rarely occurring events.

Insulin and phorbol ester stimulate initiation factor eIF-4E phosphorylation by distinct pathways in Chinese hamster ovary cells overexpressing the insulin receptor.

We have developed a one-dimensional isoelectric focusing technique to measure changes in the steady-state phosphorylation of the cap-binding initiation factor, eIF-4E. We have used a Chinese hamster ovary cell line transfected with the human insulin receptor (CHO.T cells) to study the regulation of eIF-4E phosphorylation by insulin and other stimuli.

Ablation and chemistry of meteoric materials in the atmosphere of Titan.

We compute the input of meteoric materials expected on Titan, and integrate this dust model with an ablation model and a comprehensive chemical model, investigating the effects on the atmosphere and surface. We find that a water deposition of approximately 10-100 times the expected interplanetary dust flux, or a recent large impact, is required to produce the observed CO2 abundance. Ionisation due to meteoric activity is not likely to be higher than that due to other sources.

Health for All and British health policy: a comment on the quest for "healthy public policy".

Explains that Health for All is an international extra-governmental movement that seeks to pursue equity in access to health-related resources by broadening the scope of health policy. Notes that its major principles include social participation in state decision making, inter-sectoral collaboration in policy formulation and the improvement of conditions for the disadvantaged. Points out that its local initiatives often encompass health-service professionals and practitioners as well as the voluntary sector, social services and other local authority departments, and that the effect of this local activity on political understandings of health at a national level gives some indication of the extent to which this local time and effort have been justified.

Age and experience in motorcycling safety.

Official casualty statistics show that young motorcyclists are more likely than older motorcyclists to be killed or seriously injured on the roads. We address two main issues: might the statistics be attributable to inexperience rather than youth; and might accidents be associated with particular patterns of behaviour which may themselves be predictable from riders' beliefs? From a national prospective survey of over 4000 riders in the U.K.

The role of eIF4 in cell proliferation.

Translational control has a key role in regulating cell growth. The mRNA binding translation factors (eIF4s) selectively modulate the translation of different mRNAs based on their differing properties, especially the extent of inhibitory secondary structure in their 5' untranslated regions. The mRNA 5' cap is recognized by eIF4E, which can then recruit other translation factors including the helicase cIF4A.

Acting on complaints about mental health services. Implications of power imbalances.

Complaint procedures are meant to provide an effective safeguard for users of services, but they do not always work well. Applies an analysis of three views of power to complaint procedures in mental health services. Identifies three factors which might affect the effectiveness of complaint procedures: the lack of credibility often accorded users of mental health services; the fear service users may have about complaining; and the difficulties they might experience in knowing what they can expect from a service.

Costs of antifungal prophylaxis after bone marrow transplantation. A model comparing oral fluconazole, liposomal amphotericin and oral polyenes as prophylaxis against oropharyngeal infections.

We used a costs model to compare alternative modes of prophylaxis against oropharyngeal fungal infections in patients with leukaemia or myeloma who had undergone bone marrow transplantation (BMT). We compared 2 innovative pharmaceutical options (oral fluconazole and intravenous liposomal amphotericin) with existing standard practice (oral polyenes). Costs were measured over a 12-week treatment period, and were compared with the 2 effectiveness measures: (i) the avoidance of colonisation or infection; and (ii) the patients' ability to continue with prophylaxis in an uninfected state.

Dehalogenation of haloalkanes by Rhodococcus erythropolis Y2. The presence of an oxygenase-type dehalogenase activity complements that of an halidohydrolase activity.

Rhodococcus erythropolis Y2 produced two types of dehalogenase: a hydrolytic enzyme, that is an halidohydrolase, which was induced by C3 to C6 1-haloalkane substrates, and at least one oxygenase-type dehalogenase induced by C7 to C16 1-haloalkanes and n-alkanes. The oxygenase-type activity dehalogenated C4 to C18 1-chloroalkanes with an optimum activity towards 1-chlorotetradecane. The halidohydrolase catalysed the dehalogenation of a wide range of 1- and alpha,omega-disubstituted haloalkanes and alpha,omega-substituted haloalcohols.

Vulnerability to depressive mood reactions: retesting the hopelessness model of depression.

A replication of Metalsky, Halberstadt & Abramson's (1987) test of the revised hopelessness model of depression was conducted. Depressive mood response to examination outcome was assessed in undergraduate students across two periods of examination. In phase 1, consistent with Metalsky et al.

Schizophrenia: shifting the balance of care.

Schizophrenia poses a major challenge to policy makers in health and social care in England. As deinstitutionalisation has progressed, public concern about people with the condition has increased owing to a small number of public incidents. This paper describes the overall balance of care or support for people with schizophrenia, particularly between different settings and the services received.

The research school of Marie Curie in the Paris faculty, 1907-14.

As the most famous woman scientist of the twentieth century, there has been no shortage of books and articles on the life and career of Marie Curie (1867-1934). Her role as director of a laboratory-based research school in the new scientific field of radioactivity, a field which embraced both chemistry and physics, however, has never been examined. In recent years, there has been a growing interest in the question of research schools, and Morrell, Ravetz, Geison, and Klosterman, amongst others, have written on this subject.

Immunoregulatory role of H-2 and intra-H-2 alleles on antibody responses to recombinant preparations of B-subunits of Escherichia coli heat-labile enterotoxin (rEtxB) and cholera toxin (rCtxB).

The immunoregulatory role of H-2 and intra-H-2 alleles on antibody responses to recombinant preparations of B-subunits of Escherichia coli heat-labile enterotoxin (rEtxB) and cholera toxin (rCtxB) is reported. Oral delivery of rEtxB to congenic mice of several different H-2 haplotypes resulted in H-2 dependent serum IgG responses (H-2d > H-2b = H-2q > H-2a > H-2k) and a similar spectrum of intestinal IgA responses in those strains tested. Responses to rEtxB and rCtxB were found to be differentially modulated by the H-2 locus, with significant differential effects in H-2b and H-2d congenic strains (H-2d > H-2b for rEtxB; H-2b > H-2d for rCtxB).

Monitoring respiratory activity in neonates using diaphragmatic electromyograph.

The monitoring of the development of respiratory function in low-birth-weight or sick neonates is important in its overall treatment. The most direct approach to obtaining information on respiratory function is to monitor the activity of the diaphragmatic muscular system rather than measure the resultant change in lung volume or ventilator output, which cannot be guaranteed to be directly related to the neonate's own respiratory activity. The system was designed to assess the feasibility of monitoring respiratory activity in such neonates by measuring the diaphragmatic electromyograph.

Community mental health care for former hospital in-patients. Predicting costs from needs and diagnoses.

Background: In the UK the replacement of long-term in-patient care with community-based support has been part of central government health policy for many years. One of the challenges of implementing such a policy is the prediction of support and service needs in the community and the associated costs.

Method: Using research data from north London analyses were undertaken to examine the associations between service use and costs in the community and the characteristics of hospital in-patients.

Cytoplasmic chaperonin complexes enter neurites developing in vitro and differ in subunit composition within single cells.

Chaperonins containing t-complex polypeptide-1 (CCT) are cytosolic molecular chaperone particles implicated especially in the biogenesis of cytoskeletal proteins by promoting the correct folding of the major ubiquitous cytoskeletal components, tubulin and actin. We have purified cytosolic chaperonins from the ND7/23 cell line, determined their subunit composition and examined changes in the intracellular locations of their components during differentiation of ND7/23 cells to a neuronal phenotype by using immunocytochemistry and immunoblots. Chaperonins containing the CCT alpha (TCP1) subunit enter neuritic processes and are particularly noticeable at the leading edge of growth cone-like structures where they co-localise with actin.