The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion.

Ploidy is tightly regulated in eukaryotic cells and is critical for cell function and survival. Cells coordinate multiple pathways to ensure replicated DNA is segregated accurately to prevent abnormal changes in chromosome number. In this study, we characterize an unanticipated role for the "remodels the structure of chromatin" (RSC) complex in ploidy maintenance.

Bhagavad Gita for the Physician.

This communication presents verses from the Bhagavad Gita which help define a good clinician's skills and behavior. Using the teachings of Lord Krishna, these curated verses suggest three essential skills that a physician must possess: Excellent knowledge, equanimity, and emotional attributes. Three good behaviors are listed (Pro-work ethics, Patient-centered care, and Preceptive leadership) and supported by thoughts written in the Gita.

Apical and basal epitheliomuscular F-actin dynamics during bud evagination.

Bending of 2D cell sheets is a fundamental morphogenetic mechanism during animal development and reproduction. A critical player driving cell shape during tissue bending is the actin cytoskeleton. Much of our current knowledge about actin dynamics in whole organisms stems from studies of embryonic development in bilaterian model organisms.

CCN5/WISP-2 restores ER-∝ in normal and neoplastic breast cells and sensitizes triple negative breast cancer cells to tamoxifen.

CCN5/WISP-2 is an anti-invasive molecule and prevents breast cancer (BC) progression. However, it is not well understood how CCN5 prevents invasive phenotypes of BC cells. CCN5 protein expression is detected in estrogen receptor-α (ER-α) -positive normal breast epithelial cells as well as BC cells, which are weakly invasive and rarely metastasize depending on the functional status of ER-α.

The society for craniofacial genetics and developmental biology 39th annual meeting.

The Society for Craniofacial Genetics and Developmental Biology (SCGDB) aims to promote education, research, and communication, about normal and abnormal development of the tissues and organs of the head. Membership of the SCGDB is broad and diverse-including clinicians, orthodontists, scientists, and academics-but with all members sharing an interest in craniofacial biology. Each year, the SCGDB hosts a meeting where members can share their latest research, exchange ideas and resources, and build on or establish new collaborations.

Molecular Mechanisms of Colistin Resistance in Causing Bacteremia from India-A First Report.

Colistin has long been a reserve drug used for the treatment of carbapenem resistant . Carbapenem resistance in has been increasing and is as high as 44% in India. Although a reserve agent, with rise in rates of resistance to carbapenems, the usage of colistin has increased over the years leading to slow emergence of resistance.

Rare variants in SQSTM1 and VCP genes and risk of sporadic inclusion body myositis.

Genetic factors have been suggested to be involved in the pathogenesis of sporadic inclusion body myositis (sIBM). Sequestosome 1 (SQSTM1) and valosin-containing protein (VCP) are 2 key genes associated with several neurodegenerative disorders but have yet to be thoroughly investigated in sIBM. A candidate gene analysis was conducted using whole-exome sequencing data from 181 sIBM patients, and whole-transcriptome expression analysis was performed in patients with genetic variants of interest.

Protein folding on biosensor tips: folding of maltodextrin glucosidase monitored by its interactions with GroEL.

Protein folding has been extensively studied for the past six decades by employing solution-based methods such as solubility, enzymatic activity, secondary structure analysis, and analytical methods like FRET, NMR, and HD exchange. However, for rapid analysis of the folding process, solution-based approaches are often plagued with aggregation side reactions resulting in poor yields. In this work, we demonstrate that a bio-layer interferometry (BLI) chaperonin detection system can identify superior refolding conditions for denatured proteins.

Aerobic exercise training in the treatment of non-alcoholic fatty liver disease related fibrosis.

Key Points: Physiologically relevant rodent models of non-alcoholic steatohepatitis (NASH) that resemble the human condition are limited. Exercise training and energy restriction are first-line recommendations for the treatment of NASH. Hyperphagic Otsuka Long-Evans Tokushima fatty rats fed a western diet high in fat, sucrose and cholesterol for 24 weeks developed a severe NASH with fibrosis phenotype.

The society for craniofacial genetics and developmental biology 38th annual meeting.

The mission of the Society for Craniofacial Genetics and Developmental Biology (SCGDB) is to promote education, research, and communication about normal and abnormal development of the tissues and organs of the head. The SCGDB welcomes as members undergraduate students, graduate students, post doctoral researchers, clinicians, orthodontists, scientists, and academicians who share an interest in craniofacial biology. Each year our members come together to share their novel findings, build upon, and challenge current knowledge of craniofacial biology.

Animal models for studying neural crest development: is the mouse different?

The neural crest is a uniquely vertebrate cell type and has been well studied in a number of model systems. Zebrafish, Xenopus and chick embryos largely show consistent requirements for specific genes in early steps of neural crest development. By contrast, knockouts of homologous genes in the mouse often do not exhibit comparable early neural crest phenotypes.

The effects of an intronic polymorphism in TOMM40 and APOE genotypes in sporadic inclusion body myositis.

A previous study showed that, in carriers of the apolipoprotein E (APOE) genotype ε3/ε3 or ε3/ε4, the presence of a very long (VL) polyT repeat allele in "translocase of outer mitochondrial membrane 40" (TOMM40) was less frequent in patients with sporadic inclusion body myositis (sIBM) compared with controls and associated with a later age of sIBM symptom onset, suggesting a protective effect of this haplotype. To further investigate the influence of these genetic factors in sIBM, we analyzed a large sIBM cohort of 158 cases as part of an International sIBM Genetics Study. No significant association was found between APOE or TOMM40 genotypes and the risk of developing sIBM.

Treatment-related symptom severity and occurrences among oncology adults in Australia.

Objective: Cancer treatments cause a range of distressing symptoms that can be well managed with pharmacological and nonpharmacological interventions. Treatment-related symptom screening and management by health care professionals is required to provide appropriate guidance to help patients to complete successfully their treatment regimen and achieve the best possible outcomes for patients. The aims of this study were to explore treatment-related symptom severity and occurrences among oncology adults in Australia and compare the results with the Chinese and Filipino studies.

Role of VltAB, an ABC transporter complex, in viologen tolerance in Streptococcus mutans.

Streptococcus mutans, a Gram-positive organism, is the primary causative agent in the formation of dental caries in humans. To persist in the oral cavity, S. mutans must be able to tolerate rapid environmental fluctuations and exposure to various toxic chemicals.

Role of the primary somatosensory cortex in motor learning: An rTMS study.

Somatosensation is thought to play an important role in skilled motor learning. The present study investigated how healthy adults learn a continuous implicit motor task when somatosensation is altered by 1 Hz repetitive transcranial magnetic stimulation (rTMS) delivered over the primary somatosensory cortex (S1). Twenty-seven right-handed participants enrolled in a two-part experiment.

Sexual practices of deaf and hearing secondary school students in ibadan, Nigeria.

Background: Adolescents and young people with disabilities generally face various forms of discrimination. Inspite of this, they have to deal with similar reproductive health issues encountered by their abled peers. This situation is made worse by misconceptions that they are not sexually active.

Meiotic spindle dynamics in human oocytes following slow-cooling cryopreservation.

Background: The demand for cryopreservation of human oocytes is increasing in assisted reproduction clinics and yet remains an experimental procedure. Surprisingly, little is known about the effects of cryopreservation on spindle-chromosome interactions and the recovery of meiotic spindle functionality. The goal of these studies was to evaluate the process of meiotic spindle reassembly and chromosome alignment in cryopreserved human metaphase II oocytes.

An oocentric view of folliculogenesis and embryogenesis.

The mammalian oocyte undertakes a highly complex journey to maturity during which it successively acquires a series of characteristics necessary for fertilization and the development of a healthy embryo. While the contribution of granulosa cells to oocyte development has been studied for many years, it has recently become apparent that the oocyte itself plays a key role in directing its own fate as well as the growth and differentiation of the follicle. This regulatory capacity is achieved through the synthesis and secretion of oocyte-specific factors, such as growth and differentiation factor 9 and bone morphogenetic protein 15, which act on granulosa cells to modify their proliferation, function and differentiation, as well as through direct physical contacts that occur at the granulosa cell-oocyte interface.

Relational conversation as method for creating partnerships: pilot study.

Aim: This paper reports a pilot study testing the intervention, Self-Care TALK, whose aim was to describe communication skills used by an advanced practice nurse to create partnerships with caregivers. The communication process and examples of skills used in creating partnerships are described.

Background: Decades of exploring nurse-client relationships provide a knowledge base for describing a structure and process for building partnerships.

Abnormal hemorheological properties in patients with compensated and decompensated hepatic cirrhosis.

In this study the hemorheological profiles of blood samples from cirrhotic patients (compensated and decompensated) were determined, along with 18 normal healthy volunteers for comparison. Both groups of patients were anaemic, and as a consequence had a lower native blood viscosity by comparison with the healthy control group. However, after adjusting the hematocrit to 45% the high shear rate (128 s(-1)) viscosity was within the normal range, while that at low shear rate (0.

Matrix metalloproteinases and tissue inhibitors of metalloproteinases in giant cell arteritis: an immunocytochemical study.

Giant cell arteritis (GCA) is a relatively common granulomatous arteritis of unknown etiology which mainly occurs in elderly people. Using histopathological findings from-seven biopsy cases of temporal artery and one autopsy case of GCA, and performing immunocytochemical staining for matrix metalloproteinase (MMP)-2 and -9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 and -2, we tested the hypothesis that an imbalance between MMPs and TIMPs may be a critical determinant in developing severe intimal hyperplasia and luminal stenosis. All biopsy cases revealed nearly complete luminal occlusion of the temporal artery with active lymphocytic infiltrate, fragmentation of internal lamina and median elastic fibers.

The effect of intravenous vasopressin on gastric myoelectrical activity in human subjects.

Unlabelled: Vasopressin's role in the sensation of nausea is incompletely understood. In this study, our goals were to investigate whether high intravenous vasopressin levels in normal subjects would induce nausea and vomiting and to determine the electrogastrographic (EGG) pattern which would develop at these concentrations.

Methods: EGG recordings were made on five fasting healthy subjects (three females, mean age: 27 years).

p53 and proliferating cell nuclear antigen in endocrine tumors of pancreas and intestinal carcinoids.

Twenty six endocrine tumors of the pancreas and 19 carcinoids of the intestinal tract were studied with immunocytochemical staining for p53 and proliferating cell nuclear antigen (PCNA) to detect any correlation between PCNA and p53 immunostaining. PCNA immunostaining is somewhat variable in endocrine tumors of the pancreas whereas p53 overexpression was detected in one out of eight insulinomas (12.5%), four out of eight gastrinomas (50%) and none of two glucagonomas, six PPomas and two non-functioning tumors.

Transcriptional analysis of type 1 capsule genes in Staphylococcus aureus.

Serotype 1 capsule of Staphylococcus aureus plays an important role in staphylococcal infections. A 14.6 kb chromosomal DNA fragment containing 13 cap1 genes responsible for the synthesis of type 1 capsule in S.