The effect of sucrose application and implantation of mutans streptococci on the microbial composition of three-week experimental plaque--an in situ study.

This study describes the predominant cultivable microflora of three-week-old plaque samples obtained from human enamel sites, on the basis of microbial identification of over 9000 fresh isolates. Lower removable appliances, on which were mounted enamel sections and slabs, were worn by five young adult subjects under three experimental protocols. These were (1) 'normal' plaque conditions, (2) extra-oral sucrose applications nine times daily, and (3) inoculation of each subject's own mutans streptococci onto the enamel test sites and sucrose applications, as described above.

Formation of interdental soft tissue defects after surgical treatment of periodontitis.

This study monitored the development and repair of interdental soft tissue defects following surgical treatment of periodontitis in 21 patients. Open flap curettage was performed at 100 interdental areas with follow-up examinations 1, 3, and 6 months later. Interdental gingival contours were assessed both clinically and indirectly with silicone elastomer impressions from which stone models were obtained; defect depths were then calculated using the Reflex Microscope.

Adenomatoid odontogenic tumour. A survey of 21 cases from Sri Lanka.

A series of 21 cases of adenomatoid odontogenic tumour is reported from Sri Lanka. They formed 8.6% of odontogenic tumours, excluding odontomes, in the 10-year period reviewed.

Isoelectric focusing of human parotid salivary proteins in hybrid carrier ampholyte-immobilized pH gradient polyacrylamide gels.

Isoelectric focusing of human salivary proteins with carrier ampholyte-isoelectric focusing systems requires prior desalting and concentration of samples, a procedure which is time-consuming and requires relatively large volumes of samples. By contrast, immobilized pH gradient gels are more tolerant to salt loads. Thus pretreatment of samples consists only of centrifugation prior to isoelectric focusing.

An enzyme-linked immunoassay for human salivary albumin.

An ELISA method has been developed for the determination of albumin in human parotid saliva. The range of sensitivity is 2-80 micrograms/l and it is suitable for use with volumes of 5 microliters or less of freshly collected parotid fluid, without prior sample preparation. Calibration data were fitted by microcomputer to an exponential function.

Systemic metronidazole in the treatment of periodontitis.

The present study evaluated the effect of systemic metronidazole on advanced periodontitis in 10 patients with inadequate oral hygiene. Clinical and microbiological observations were made at a total of 173 bleeding pockets of 5 mm depth or more. The clinical observations comprised plaque index scores, dichotomous measurements of gingival redness and suppuration, pocket depths and attachment levels.

Comparison of solution- and gel-prepared enamel lesions--and in vitro pH-cycling study.

A variety of methods has been employed to produce artificial caries-like enamel lesions. The aim of this paper was to use a pH-cycling regime to compare the de-/remineralization behavior of lesions prepared by two methods. Lesions were produced by use of either an acidified undialyzed gelatin system or a buffered solution.

Longitudinal study of untreated periodontitis (II). Microbiological findings.

11 volunteer subjects with advanced chronic periodontitis participated in a 1-year longitudinal clinical and microbiological study. Subgingival plaque was collected at each of 7 visits from 148 pre-selected sites in the left jaw quadrants (test sites) and on the first and last visits, only from 117 sites in the left jaw quadrants (control sites). All sites were examined clinically at each of the 7 visits, and the microbiological markers investigated were the % spirochaetes and % black pigmented Bacteroides species in subgingival plaque.

Longitudinal study of untreated periodontitis (I). Clinical findings.

The principal aim of this study was to investigate the use of certain clinical and microbiological criteria to predict periodontal breakdown during a 1-year period. A further aim was to establish whether the act of collecting subgingival plaque samples periodically throughout the observation period would have any effect on the clinical or microbiological variables. Only the clinical data is presented in this paper.