Self-awareness of deficits in Parkinson disease.

Anosognosia is an unawareness or denial of deficits. While it has mainly been associated with damage to cortical brain regions, anosognosia has also been reported in patients with subcortical brain disease. The present study investigated whether anosognosia is a feature of Parkinson disease.

Sural nerve evoked responses in human hip and ankle muscles while standing.

The objective of this study was to investigate the influence of background EMG, as manipulated by changes in limb loading, and electrical stimulus intensity on sural nerve evoked EMG responses in proximal hip (ipsilateral gluteus medius and contralateral adductor longus) and distal ankle (ipsilateral soleus) muscles during quiet standing. We studied 14 healthy subjects who were instructed to stand on a force platform and load the right leg to 20, 30, 60, and 80% body weight. Trains of stimuli were delivered to the right sural nerve at three different intensities (1.

Effects of ketamine on electroencephalographic and autonomic arousal and segmental reflex responses in the cat.

Objective: To provide evidence concerning doses of ketamine that affect electroencephalographic (EEG) and autonomic signs of arousal during nociceptive stimulation.

Study Design: Prospective psychophysical test in people. Single injection or progressively increasing infusions of ketamine in cats.

Enhancement of serotoninergic function - a sometimes insufficient cause of antidepressant action.

No Abstract

Gene therapy in cardiovascular disease. Current status.

Cardiovascular disease is the leading cause of mortality and morbidity in developed countries. Most conventional therapy is often inefficacious and tends to treat the symptoms rather than the underlying causes of the disorder. Gene therapy offers a novel approach for prevention and treatment of cardiovascular diseases.

The effect of foot placement on sit to stand in healthy young subjects and patients with hemiplegia.

Objective: To determine the effect of altering the foot placement of the dominant limb in young healthy subjects and the uninvolved limb of subjects with hemiplegia on their ability to perform sit to stand (STS).

Design: Controlled biomechanical experiment.

Setting: Research laboratory of a university health science center.

Regeneration of axons after nerve transection repair is enhanced by degradation of chondroitin sulfate proteoglycan.

Our past work indicates that growth-inhibiting chondroitin sulfate proteoglycan (CSPG) is abundant in the peripheral nerve sheaths and interstitium. In this study we tested if degradation of CSPG by chondroitinase enhances axonal regeneration through the site of injury after (a) nerve crush and (b) nerve transection and coaptation. Adult rats received the same injury bilaterally to the sciatic nerves and then chondroitinase ABC was injected near the injury site on one side, and the contralateral nerve was injected with vehicle alone.

Rhombencephalosynapsis with massive hydrocephalus: case report and pathogenetic considerations.

Rhombencephalosynapsis (RS) is a rare cerebellar malformation characterized by vermian agenesis or hypogenesis, fusion of the hemispheres, and closely apposed or fused dentate nuclei. This report concerns a 29-year-old Caucasian female with a history of profound retardation and chronic hydrocephalus. Multiple shunts had been placed and there had been several admissions for shunt infections.

Genetic targeting for cardiovascular therapeutics: are we near the summit or just beginning the climb?

This article is based on an Experimental Biology symposium held in April 2001 and presents the current status of gene therapy for cardiovascular diseases in experimental studies and clinical trials. Evidence for the use of gene therapy to limit neointimal hyperplasia and confer myocardial protection was presented, and it was found that augmenting local nitric oxide (NO) production using gene transfer (GT) of NO synthase or interruption of cell cycle progression through a genetic transfer of cell cycle regulatory genes limited vascular smooth muscle hyperplasia in animal models and infra-inguinal bypass patients. The results of application of vascular endothelial growth factor (VEGF) GT strategies for therapeutic angiogenesis in critical limb and myocardial ischemia in pilot clinical trials was reviewed.

Verbal encoding deficits in a patient with a left retrosplenial lesion.

Over the past decade, memory impairments associated with retrosplenial damage have received increased attention among neuroscientists, although the exact role of the retrosplenial region in memory has not been clearly defined. Evidence from lesion studies and functional neuroimaging has implicated the retrosplenial region in verbal episodic memory, temporal ordering of information, and topographical memory. In addition, recent positron emission tomography studies have shown increased activation of the retrosplenial cortex during tasks involving both the encoding and retrieval of episodic information.

Crosstalk between the norepinephrine and serotonin systems and its role in the antidepressant response.

Many behavioural overlaps exist in the effects of norepinephrine (NE), serotonin (5-HT) and dopamine, and it is now thought that complex behaviour patterns may reflect interactions among these neurotransmitters. There is a wide variety of evidence for the pivotal role of the NE system in the pathogenesis and treatment of major depression. This paper discusses the functioning of the NE system, specifically the regulation of neuronal firing and the postsynaptic responses to NE, which can be controlled by norepinephrine reuptake inhibitors and other drugs.

Axonal regeneration into acellular nerve grafts is enhanced by degradation of chondroitin sulfate proteoglycan.

Although the peripheral nerve has the potential to regenerate after injury, degenerative processes may be essential to promote axonal growth into the denervated nerve. One hypothesis is that the nerve contains growth inhibitors that must be neutralized after injury for optimal regeneration. In the present study, we tested whether degradation of chondroitin sulfate proteoglycan, a known inhibitor of axon growth, enhances the growth-promoting properties of grafts prepared from normal donor nerves.

Lateralization of temporal lobe foci: depth versus subdural electrodes.

Objectives: Definitive localization of an epileptic focus correlates with a favorable outcome following epilepsy surgery. This study was undertaken to determine the incremental value of data yielded for surgical decision making when using subdural electrodes alone and in addition to depth electrodes for temporal lobe epilepsy.

Methods: Standardized placement for intracranial electrodes included: (1) longitudinal placement of bilateral temporal lobe depth electrodes; (2) bilateral subtemporal subdural strips; and (3) bilateral orbitofrontal subdural strips.

Visualization of neural tissue water compartments using biexponential diffusion tensor MRI.

The apparent diffusion tensor (ADT) imaging method was extended to account for multiple diffusion components. A biexponential ADT imaging experiment was used to obtain separate images of rapidly and slowly diffusing water fractions in excised rat spinal cord. The fast and slow component tensors were compared and found to exhibit similar gross features, such as fractional anisotropy, in both white and gray matter.

Estrogens decrease reperfusion-associated cortical ischemic damage: an MRI analysis in a transient focal ischemia model.

Background And Purpose: Early identification of irreversible cerebral ischemia is critical in defining strategies that influence neuronal survival after stroke. We used MRI to investigate the effects of 17beta-estradiol (E2) on the temporal evolution of focal ischemia.

Methods: Female rats were ovariectomized and divided into 1 of 2 groups: ovariectomy alone (OVX; n=4) or ovariectomy with estrogen replacement (OVX+E2; n=3).

Anatomical risk factors for phonological dyslexia.

Successful behavioral genetic studies require precise definition of a homogenous phenotype. This study searched for anatomical markers that might restrict variability in the reading disability phenotype. The subjects were 15 college students (8 male/7 female) diagnosed with a reading disability (RD) and 15 controls (8 males/7 females).

Tumorigenic properties of neurofibromin-deficient neurofibroma Schwann cells.

Dermal and plexiform neurofibromas are peripheral nerve sheath tumors that arise frequently in neurofibromatosis type 1. The goal of the present study was to examine the tumorigenic properties of neurofibromin-deficient human Schwann cells (SCs) that were found to represent a subset of SCs present in approximately half of the total neurofibromas examined. Highly enriched SC cultures were established from 10 dermal and eight plexiform neurofibromas by selective subculture using glial growth factor-2 and laminin.

Distribution of P2X1, P2X2, and P2X3 receptor subunits in rat primary afferents: relation to population markers and specific cell types.

We determined the co-expression of immunoreactivity (IR) for ATP-receptor subunits (P2X1, P2X2, and P2X3), neuropeptides, neurofilament (NF), and binding of the isolectin B(4) from Griffonia simplicifolia type one (GS-I-B(4)) in adult dorsal root ganglion neurons. P2X1-IR was expressed primarily in small DRG neurons. Most P2X1-IR neurons expressed neuropeptides and/or GS-I-B(4)-binding, but lacked NF-IR.

Use of antisense oligodeoxynucleotides to study the physiological functions of neuropeptide Y.

Stimulation of appetite and regulation of reproductive hormone secretion are two well-known physiological effects of neuropeptide Y (NPY) that have been affirmed using the antisense oligodeoxynucleotide (ODN) approach. Because NPY-producing neurons are concentrated in a narrow band in the arcuate nucleus of the hypothalamus, ODNs injected intracerebroventricularly have easy access to them. In an early study intracerebroventricular administration of an unmodified phosphodiester ODN sequence blocked de novo NPY synthesis and prevented the preovulatory surge release of gonadotropins.

Attenuation of hypertension by systemic delivery of retroviral vector containing type I angiotensin II receptor antisense cDNA.

Despite recent strides in the traditional pharmacological therapies in the control and management of hypertension, a successful prevention and cure for this disease by conventional drug strategy remain at a standstill. We have begun to investigate the conceptual possibility of the use of gene therapy in the control of hypertension. In this article we describe an experimental protocol that provides proof of the principle that antisense (AS) inhibition of Type I angiotensin II receptor (AT(1)-R) could prevent development of hypertension on a long-term basis.

Angiotensin II increases neuronal delayed rectifier K(+) current: role of 12-lipoxygenase metabolites of arachidonic acid.

Angiotensin II (Ang II) elicits an Ang II type 2 (AT(2)) receptor-mediated increase in voltage-dependent delayed rectifier K(+) current (I(KV)) in neurons cultured from newborn rat hypothalamus and brain stem. In previous studies, we have determined that this effect of Ang II is mediated via a Gi protein, activation of phospholipase A(2) (PLA(2)), and generation of arachidonic acid (AA). AA is rapidly metabolized within cells via lipoxygenases (LO), cyclooxygenase (COX) or p450 monooxygenase enzymes, and the metabolic products are known regulators of K(+) currents and channels.