Know your limits; miniCOI metabarcoding fails with key marine zooplankton taxa.

Eleven years after the publication of the first work applying deoxyribonucleic acid (DNA) metabarcoding to zooplankton communities, the commonly known "miniCOI" barcode is widely used, becoming the marker of choice. However, several primer combinations co-exist for this barcode and a critical evaluation of their performance is needed. This article reviews the misperformance of miniCOI metabarcoding with marine zooplankton communities, comparing them to microscopy and/or other universal markers.

Dragonflies (Odonata) as bioindicators of radioactivity.

Bioindicators are living organisms that are successfully used for monitoring changes in the environmental health due to natural and/or anthropogenic influences. Dragonflies (Odonata) are considered to be good indicators of water quality; however, research on dragonflies as potential indicators of radioactivity is scarce. The aim of this study was to evaluate dragonflies as potential biological indicators of ionising radiation in nature by measuring Cs activity concentrations in the dragonfly and river water samples.

Historical dataset details the distribution, extent and form of lost Ostrea edulis reef ecosystems.

Ocean ecosystems have been subjected to anthropogenic influences for centuries, but the scale of past ecosystem changes is often unknown. For centuries, the European flat oyster (Ostrea edulis), an ecosystem engineer providing biogenic reef habitats, was a culturally and economically significant source of food and trade. These reef habitats are now functionally extinct, and almost no memory of where or at what scales this ecosystem once existed, or its past form, remains.

Marine plankton community and net primary production responding to island-trapped waves in a stratified oligotrophic ecosystem.

The oligotrophic Adriatic Sea is characterized during a typical summer by low productivity caused by strong water column stratification, which inhibits vertical mixing and nutrient supply to the euphotic zone. These conditions can be disrupted by transient physical forcing, which enhances nutrient fluxes and creates localized hotspots of relatively high net primary production. In this study, plankton abundance and diversity were investigated in relation to the physical forcing and nutrient concentrations in an area affected by island-trapped waves (ITWs) near Lastovo Island (Adriatic Sea).

Supervised learning-based artificial senses for non-destructive fish quality classification.

Human sensory techniques are inadequate for automating fish quality monitoring and maintaining controlled storage conditions throughout the supply chain. The dynamic monitoring of a single quality index cannot anticipate explicit freshness losses, which remarkably drops consumer acceptability. For the first time, a complete artificial sensory system is designed for the early detection of fish quality prediction.

A Dataset for Detection and Segmentation of Underwater Marine Debris in Shallow Waters.

Robust object detection is crucial for automating underwater marine debris collection. While supervised deep learning achieves state-of-the-art performance in discriminative tasks, replicating this success on underwater data is challenging. The generalization of these methods suffers due to a lack of available annotated data considering different sources of variation in the unstructured underwater environment and imaging conditions.

Performance of mussel Mytilus galloprovincialis under variable environmental conditions and anthropogenic pressure: A survey of two distinct farming sites in the Adriatic Sea.

Changes in natural conditions and anthropogenic pollutants, alone or in combination, pose a significant challenge to coastal bivalve populations. The susceptibility of economically important bivalves to potential stressors in their farming environment has not been sufficiently investigated, despite the increase in anthropogenic pressure along the coast and the remarkable warming of seawater in recent years. Thus, the aim of this study was to evaluate the performance of mussel (Mytilus galloprovincialis) from two important farming sites in the eastern Adriatic, namely Mali Ston Bay (MSB) and Lim Bay (LB), in relation to variations of seawater parameters, reproductive cycle dynamics and tissue content of potentially harmful pollutants.

Laboratory medicine and sports: where are we now?

Laboratory medicine in sport and exercise has significantly developed during the last decades with the awareness that physical activity contributes to improved health status, and is present in monitoring both professional and recreational athletes. Training and competitions can modify concentrations of a variety of laboratory parameters, so the accurate laboratory data interpretation includes controlled and known preanalytical and analytical variables to prevent misleading interpretations. The paper represents a comprehensive summary of the lectures presented during the 35 Annual Symposium of the Croatian Society of Medical Biochemistry and Laboratory Medicine.

Evolutionary Insights from the Mitochondrial Genome of Oikopleura dioica: Sequencing Challenges, RNA Editing, Gene Transfers to the Nucleus, and tRNA Loss.

Sequencing the mitochondrial genome of the tunicate Oikopleura dioica is a challenging task due to the presence of long poly-A/T homopolymer stretches, which impair sequencing and assembly. Here, we report on the sequencing and annotation of the majority of the mitochondrial genome of O. dioica by means of combining several DNA and amplicon reads obtained by Illumina and MinIon Oxford Nanopore Technologies with public RNA sequences.

The Use of a Natural Polysaccharide Extracted from the Prickly Pear Cactus () as an Additive for Textile Dyeing.

The art of dyeing fabrics is one of the oldest human activities. In order to improve the fastness properties of dyeing products, various additives are added to optimize the uniformity of fibers and surfaces and improve dye distribution. Unfortunately, these additives can be harmful and very often are not biodegradable.

Radiocaesium in mosses from the Kopački rit Nature Park in Croatia: searching for undeclared releases from nuclear facilities in war-torn Ukraine.

The invasion of Ukraine and military operations around Ukrainian nuclear power plants and other nuclear facilities have prompted us to search for radiocaesium in mosses from the Kopački Rit Nature Park in Croatia, since mosses are known bioindicators of airborne radioactive pollution, and Kopački Rit is a known low radiocaesium background area. Sampling was finished in August 2023, and our analysis found no elevated radiocaesium levels. Kopački Rit therefore remains a suitable place for future detection of anthropogenic radioactive pollutants.

What is pre-COPD and do we know how to treat it?

Introduction: Chronic Obstructive Pulmonary Disease (COPD) is one of the leading causes of morbidity and mortality worldwide. The lung damage in COPD is associated with an enhanced chronic inflammatory response in the airways and lung tissue to harmful particles or gases. Early detection and treatment of COPD can help manage symptoms and slow the progression of the disease.

A Multi-Agent System for Service Provisioning in an Internet-of-Things Smart Space Based on User Preferences.

The integration of the Internet of Things (IoT) and artificial intelligence (AI) is critical to the advancement of ambient intelligence (AmI), as it enables systems to understand contextual information and react accordingly. While many solutions focus on user-centric services that provide enhanced comfort and support, few expand on scenarios in which multiple users are present simultaneously, leaving a significant gap in service provisioning. To address this problem, this paper presents a multi-agent system in which software agents, aware of context, advocate for their users' preferences and negotiate service settings to achieve solutions that satisfy everyone, taking into account users' flexibility.

The Influence of Tear Film Quality on Visual Function.

Background: The prevalence of dry eye disease (DED) is increasing globally, resulting in a variety of eye symptoms characterized by discomfort and visual disturbances. The accurate diagnosis of the disease is often challenging and complex, requiring specialized diagnostic tools. This study aimed to investigate the impact of tear film instability on visual function and to evaluate the value of post-blink blur time (PBBT) as an alternative method for assessing tear film stability.

Global warming scenarios for the Eastern Adriatic Sea indicate a higher risk of invasiveness of non-native marine organisms relative to current climate conditions.

Unlabelled: Globally, marine bioinvasions threaten marine ecosystem structure and function, with the Mediterranean Sea being one of the most affected regions. Such invasions are expected to increase due to climate change. We conducted a risk screening of marine organisms (37 fishes, 38 invertebrates, and 9 plants), both extant and 'horizon' (i.

Reversible splenial lesion syndrome in patient with acute motor and sensory axonal neuropathy.

Even though the classical clinical concept supports the clear difference between diseases affecting the central and peripheral nervous systems, this difference is becoming less rigid. Here, we report the case of a 50-year-old male patient who presented with acroparaesthesia, headache, and flaccid tetraparesis after febrile diarrhea. Nerve conduction studies indicated action potentials with low amplitudes, which are typical in acute motor and sensory axonal neuropathy.

Levels of depression, anxiety and subjective happiness among health sciences students in Croatia: a multi-centric cross-sectional study.

Background: Previous studies have shown that symptoms of depression and anxiety were highly prevalent among health sciences students. This may lead to other professional and personal difficulties and a decrease in individuals' well-being. This study aimed to analyze levels of depression, anxiety and subjective happiness among health sciences students in Croatia.

Breathlessness and "exacerbation" questions predictive for incident COPD (MARKO study): data after two years of follow-up.

Aims: To determine the predictability of the MARKO questionnaire and/or its domains, individually or in combination with other markers and characteristics (age, gender, smoking history, lung function, 6-min walk test (6 MWT), exhaled breath temperature (EBT), and hsCRP for the incident chronic obstructive pulmonary disease (COPD) in subjects at risk over 2 years follow-up period).

Participants And Methods: Patients, smokers/ex-smokers with >20 pack-years, aged 40-65 years of both sexes were recruited and followed for 2 years. After recruitment and signing the informed consent at the GP, a detailed diagnostic workout was done by the pulmonologist; they completed three self-assessment questionnaires-MARKO, SGRQ and CAT, detailed history and physical, laboratory (CBC, hsCRP), lung function tests with bronchodilator and EBT.

Benthic Diatom Blooms of Blue spp. in the Mediterranean Sea.

Blue species are marine benthic pennate diatoms able to synthesize a blue-green water-soluble pigment, like marennine produced by Simonsen. New species of synthetizing blue pigments were recently described (, , and ). Their marennine-like pigments have allelopathic, antioxidative, antiviral and antibacterial properties, which have been demonstrated in laboratory conditions.

Tear Biomarkers and Alzheimer's Disease.

Alzheimer's disease (AD) is an age-related progressive neurodegenerative brain disorder that represents the most common type of dementia. It poses a significant diagnostic challenge that requires timely recognition and treatment. Currently, there is no effective therapy for AD; however, certain medications may slow down its progression.

Botanical Analysis of the Baroque Art on the Eastern Adriatic Coast, South Croatia.

The analysis of plants featured in Baroque artworks on the eastern Adriatic coast has not previously been the subject of an in-depth study. The study of plant iconography in Baroque sacred artworks, which are mostly paintings, was carried out in eight churches and monasteries on the Pelješac peninsula in southern Croatia. Taxonomic interpretation of the painted flora on 15 artworks led to the identification of 23 different plant taxa (species or genera) belonging to 17 families.

Evolution of Hybrid LiFi-WiFi Networks: A Survey.

Given the growing number of devices and their need for internet access, researchers are focusing on integrating various network technologies. Concerning indoor wireless services, a promising approach in this regard is to combine light fidelity (LiFi) and wireless fidelity (WiFi) technologies into a hybrid LiFi and WiFi network (HLWNet). Such a network benefits from LiFi's distinct capability for high-speed data transmission and from the wide radio coverage offered by WiFi technologies.