Rapid In-Field Detection of Airborne Pathogens Using Loop-Mediated Isothermal Amplification (LAMP).

Multiple human and plant pathogens are dispersed and transmitted as bioaerosols (e.g., , SARS-CoV-2, , , spp.

Mechanical Behavior of 3D-Printed Zig-Zag Honeycomb Structures Made of BASF Ultrafuse 316L.

The aim of this study is to determine the mechanical behavior of 2D honeycomb cellular structures with deformation initiators subject to quasi-static compression testing. Two different loading directions were studied: in-plane (IP) and out-of-plane (OP). The deformation initiators sought to stabilize the mechanical response by decreasing the initial peak force in the case of OP loading.

Characteristics of the Outdoor Environment Affording Physical Activity, Motor Competence, and Social Interactions in Children Aged 3-7 Years: A Systematic Review.

Unlabelled: Early childhood education (ECE) settings play a crucial role in promoting physical and social development among children aged 3-7 years. This systematic review sought to examine the associations between characteristics of ECE outdoor environments, social interactions, physical activity, and motor competence. The secondary aim examines previously applied methods to capture children's behaviour in the context of their social and physical environment.

Impact of inter-city interactions on disease scaling.

Inter-city interactions are critical for the transmission of infectious diseases, yet their effects on the scaling of disease cases remain largely underexplored. Here, we use the commuting network as a proxy for inter-city interactions, integrating it with a general scaling framework to describe the incidence of seven infectious diseases across Brazilian cities as a function of population size and the number of commuters. Our models significantly outperform traditional urban scaling approaches, revealing that the relationship between disease cases and a combination of population and commuters varies across diseases and is influenced by both factors.

Assessment of dynamics of pathogenic environmental T4 and T9 genotypes isolated from three recreational lakes in Klang Valley, Malaysia over the HaCaT cell monolayer.

Free-living amoebae of the genus are causative agents of keratitis and amoebic encephalitis. They are widely found in various ecological environments. Therefore, the present study brings results that can help to better understand the genotypes of the environmental isolates and their pathogenicity.

Determining the outcome measures and clinical relevance of respiratory muscle training with multiple sclerosis patients: a systematic review.

The following systematic review aimed to gather information on the effectiveness of Respiratory Muscle Training (RMT) with Multiple Sclerosis (MS) patients. The method followed the ENTREQ and PRISMA protocol. MEDLINE, Cochrane, and Science Direct databases were used to source relevant literature.

Respiratory Rate Monitoring via a Fibre Bragg Grating-Embedded Respirator Mask with a Wearable Miniature Interrogator.

A respiration rate (RR) monitoring system was created by integrating a Fibre Bragg Grating (FBG) optical fibre sensor into a respirator mask. The system exploits the sensitivity of an FBG to temperature to identify an individual's RR by measuring airflow temperature variation near the nostrils and mouth. To monitor the FBG response, a portable, battery-powered, wireless miniature interrogator system was developed to replace a relatively bulky benchtop interrogator used in previous studies.

The Role of the Gut Microbiome in the Development and Progression of Type 2 Diabetes and Liver Disease.

Type 2 diabetes mellitus (T2DM) and progressive liver disease are 2 of the most significant global health concerns, and they have alarming and ever-increasing prevalence. A growing body of literature has demonstrated a potential multilateral link between gut microbiome dysbiosis and the development and progression of the above-mentioned conditions. Modulation of gut microbial composition from the norm is due to changes in diet allied with external factors such as age, genetics, and environmental changes.

Correlation Between Self-reported or Supervised Physical Activity in Noncommunicable Diseases and Comorbidities During COVID-19 Pandemic: A Systematic Review.

Objective: The aim of the study is to systematically identify the protective and ameliorative effects of physical activity among people with noncommunicable diseases such as diabetes, cancer, chronic respiratory disease, chronic vascular disease, hypertension, and existing comorbidities during the coronavirus disease (COVID-19) pandemic.

Methods: The trial is registered in the PROSPERO registry and used the PICO (population, intervention, comparison, and outcomes) strategy to perform the search strategy. Assessors analyzed related studies in the MEDLINE, PROQUEST, PubMed, Cochrane Library, CINAHL, Embase, Google Scholar, Physiotherapy Evidence, and Science Direct databases between December 2022 and January 2023.

Machine learning based tuberculosis (ML-TB) health predictor model: early TB health disease prediction with ML models for prevention in developing countries.

Background: Tuberculosis (TB) remains one of the top infectious killers in the world and a prominent fatal disease in developing countries. This study proposes a prototypical solution to early prevention of TB based on its primary symptoms, signs, and risk factors, implemented by means of machine learning (ML) predictive algorithms. Further novelty of the study lies in the uniqueness of patient dataset collected from three top-ranked hospitals of Sindh, Pakistan, a self-administered survey patient-records that comprises a set of questions asked by the doctors treating TB patients in real-time.

'One of the greatest injustices of our time': The impact of social representations of modern slavery in the UK-A mixed methods approach.

This study aimed to examine how social representations of modern slavery and immigration become entangled in newspaper media. 2672 UK newspaper articles were collated from 2013 to 2022 and analysed using Content Analysis (Descendant Hierarchical Classification) and Critical Discourse Analysis (CDA). Two themes and corresponding extracts were identified from the content analysis output and analysed using CDA allowing for the exploration of the role of the hegemonic social representations to understanding how discourses of modern slavery are reproduced through the othering in relation to ethnicity and migration.

Raw data and noise in spectrophotometry.

Background: Spectrophotometers are ubiquitous in chemical and biological science; however, their precision limits are under-appreciated. Rules-of-thumb and IUPAC referenced guidance restricting absorbance ranges to minimize uncertainty are based on historically important instruments which are no longer as widely used. Advances over the last half-century changed most "raw" data from absorbance and transmittance values directly produced in analog electronics to digitized intensities.

Cast from the Past? Microbial Diversity of a Neolithic Stone Circle.

We studied the microbial diversity colonizing limestone rock pools at a Neolithic Monument (Arbor Low, Derbyshire, England). Five pools were analyzed: four located at the megaliths of the stone circle and one pool placed at the megalith at the Gib Hill burial mound 300 m distant. Samples were taken from rock pool walls and sediments, and investigated through molecular metabarcoding.

Physical Literacy and Physical Activity in Early Years Education: What's Known, What's Done, and What's Needed?

Physical literacy (PL) is increasingly recognised as essential for fostering lifelong engagement in physical activity (PA), particularly when nurtured in early childhood. Yet there remains limited understanding of how stakeholders in early years (EY) education perceive, value, and implement a PL-informed approach. This study aims to explore knowledge and beliefs regarding PL and PA in relation to 3-5-year-olds, investigating key questions around perceived importance, current practices, and barriers to implementation.

Drug modifications: graphene oxide-chitosan loading enhanced anti-amoebic effects of pentamidine and doxycycline.

Acanthamoeba castellanii is the causative pathogen of a severe eye infection, known as Acanthamoeba keratitis and a life-threatening brain infection, named granulomatous amoebic encephalitis. Current treatments are problematic and costly and exhibit limited efficacy against Acanthamoeba parasite, especially the cyst stage. In parallel to drug discovery and drug repurposing efforts, drug modification is also an important approach to tackle infections, especially against neglected parasites such as free-living amoebae: Acanthamoeba.

Vaccine Logistics Management Information System at Public Health Facilities in Amhara Region, Ethiopia: A Performance Evaluation.

Objectives: This study aimed to evaluate the performance of vaccine logistics management information systems at public health facilities in Amhara Region, Ethiopia.

Methods: An institution-based concurrent explanatory mixed design was used at public health facilities in Amhara Region, Ethiopia. In total, 102 health facilities were selected using the multi-stage, stratified, random-sampling proportionate-to-size method.

Application of DSP2 for biological sex estimation in a Spanish sample: analysis based on sex and side.

Applying existing sexing methodologies to different populations, and reporting these findings is important to enhance their applicability and accuracy in real cases across the world. DSP was published in 2005 (Murail et al., 2005) and updated as a DSP2 in 2017 (Brůžek et al.

Cell cycle traverse rate predicts long-term outcomes in a multi-institutional cohort of patients with triple-negative breast cancer.

Background: Ki67 index (KI) and mitotic index (MI) are proliferation markers with established prognostic value in breast carcinomas. While KI is evaluated immunohistochemically and reported as a percentage, MI is determined visually and reflects total mitotic cells in 10 high-power fields. Our objective was to integrate KI and MI into a novel metric; the cell cycle traverse rate (CCTR).

Targeting pathogenic Acanthamoeba castellanii using DNA minor groove binding agents.

DNA minor groove binders exhibit a high degree of sequence specificity and have a variety of biological actions including antiviral, anticancer, antibacterial, and anti-protozoal properties. Since it is the location of non-covalent interactions, the minor groove of double helical B-DNA is gaining significant interest as therapeutic targets. For the purpose of this investigation, the synthesis of five novel DNA minor groove binding agents was accomplished and antiparasitic efficacies were determined against Acanthamoeba castellanii of the T4 genotype in vitro.

Mental health nursing students and generic proficiencies: how educators can support assessment.

Recent shifts in nursing education towards generic proficiencies pose challenges to the unique demands of mental health nursing education. Student mental health nurses at the authors' higher education institution (HEI) have provided service feedback that has expressed the difficulties they have encountered. This article examines the issues faced by students, assessors and educators in interpreting and applying the proficiency standards set by the Nursing and Midwifery Council.