10 results match your criteria: "University of Copenhagen DK-2200[Affiliation]"

Article Synopsis
  • Many pregnant women hesitate to take oral iron supplements due to fears of gastrointestinal (GI) side effects, leading to a study comparing the effects of three different iron formulas on GI complaints in healthy pregnant women.* -
  • Two double-blind studies were conducted, involving a total of 482 women, to assess side effects like nausea, constipation, and black stools from different iron dosages and formulas during pregnancy.* -
  • Among the iron supplements tested, ferrous bisglycinate at 25 mg showed the least GI side effects, while higher doses of ferrous fumarate resulted in more constipation and black stools, indicating it might be the least preferable option for pregnant women.*
View Article and Find Full Text PDF

Local extracellular K in cortex regulates norepinephrine levels, network state, and behavioral output.

Proc Natl Acad Sci U S A

October 2023

Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen DK-2200, Copenhagen N, Denmark.

Extracellular potassium concentration ([K]) is known to increase as a function of arousal. [K] is also a potent modulator of transmitter release. Yet, it is not known whether [K] is involved in the neuromodulator release associated with behavioral transitions.

View Article and Find Full Text PDF

Covalently acting compounds experience a strong interest within chemical biology both as molecular probes in studies of fundamental biological mechanisms and/or as novel drug candidates. In this context, the identification of new classes of reactive groups is particularly important as these can expose novel reactivity modes and, consequently, expand the ligandable proteome. Here, we investigated the electrophilic reactivity of the 3-acyl-5-hydroxy-1,5-dihydro-2-pyrrole-2-one (AHPO) scaffold, a heterocyclic motif that is present in various bioactive natural products.

View Article and Find Full Text PDF

By screening of a collection of 50 000 small-molecule compounds, we recently identified 4-arylazo-3,5-diamino-1-pyrazoles as a novel group of anti-biofilm agents. Here, we report a SAR study based on 60 analogues by examining ways in which the pharmacophore can be further optimized, for example, substitutions in the aryl ring. The SAR study revealed the very potent anti-biofilm compound 4-(2-(2-fluorophenyl)hydrazineylidene)-5-imino-4,5-dihydro-1-pyrazol-3-amine ().

View Article and Find Full Text PDF

Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins.

Nucleic Acids Res

July 2019

Department of Cancer and Inflammation Research, Institute for Molecular Medicine, University of Southern Denmark, J.B. Winsløws Vej 25, DK-5000 Odense, Denmark.

Rearrangement of the 1q12 pericentromeric heterochromatin and subsequent amplification of the 1q arm is commonly associated with cancer development and progression and may result from epigenetic deregulation. In many premalignant and malignant cells, loss of 1q12 satellite DNA methylation causes the deposition of polycomb factors and formation of large polycomb aggregates referred to as polycomb bodies. Here, we show that SSX proteins can destabilize 1q12 pericentromeric heterochromatin in melanoma cells when it is present in the context of polycomb bodies.

View Article and Find Full Text PDF

Functional implications of the p.Cys680Arg mutation in the MLH1 mismatch repair protein.

Mol Genet Genomic Med

July 2014

Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen DK-2200, Copenhagen N, Denmark.

In clinical genetic diagnostics, it is difficult to predict whether genetic mutations that do not greatly alter the primary sequence of the encoded protein causing unknown functional effects on cognate proteins lead to development of disease. Here, we report the clinical identification of c.2038 T>C missense mutation in exon 18 of the human MLH1 gene and biochemically characterization of the p.

View Article and Find Full Text PDF

Many limb movements are composed of alternating flexions and extensions. However, the underlying spinal network mechanisms remain poorly defined. Here, we show that the intensity of synaptic excitation and inhibition in limb motoneurons varies in phase rather than out of phase during rhythmic scratchlike network activity in the turtle.

View Article and Find Full Text PDF

Water transport by GLUT2 expressed in Xenopus laevis oocytes.

J Physiol

March 2007

Nordic Centre for Water Imbalance Related Disorders, Department of Medical Physiology, The Panum Institute, Blegdamsvej 3C, University of Copenhagen DK-2200 N, Denmark.

The glucose transporter GLUT2 has been shown to also transport water. In the present paper we investigated the relation between sugar and water transport in human GLUT2 expressed in Xenopus oocytes. Sugar transport was determined from uptakes of non-metabolizable glucose analogues, primarily 3-O-methyl-D-glucopyranoside; key experimental results were confirmed using D(+)-glucose.

View Article and Find Full Text PDF

Two active molecular phenotypes of the tachykinin NK1 receptor revealed by G-protein fusions and mutagenesis.

J Biol Chem

June 2001

Laboratory for Molecular Pharmacology, Department of Pharmacology, The Panum Institute, University of Copenhagen DK-2200 and the 7TM Pharma A/S, DK-2100 Copenhagen, Denmark.

The NK1 neurokinin receptor presents two non-ideal binding phenomena, two-component binding curves for all agonists and significant differences between agonist affinity determined by homologous versus heterologous competition binding. Whole cell binding with fusion proteins constructed between either Galpha(s) or Galpha(q) and the NK1 receptor with a truncated tail, which secured non-promiscuous G-protein interaction, demonstrated monocomponent agonist binding closely corresponding to either of the two affinity states found in the wild-type receptor. High affinity binding of both substance P and neurokinin A was observed in the tail-truncated Galpha(s) fusion construct, whereas the lower affinity component was displayed by the tail-truncated Galpha(q) fusion.

View Article and Find Full Text PDF

PACAP-(1-38) as neurotransmitter in the porcine adrenal glands.

Am J Physiol Endocrinol Metab

December 2000

Department of Medical Physiology, The Panum Institute, University of Copenhagen DK 2200, Denmark.

The concentration of pituitary adenylyl cyclase-activating polypeptide [PACAP-(1-38)] in porcine adrenal glands amounted to 14 +/- 3 pmol/g tissue. PACAP immunoreactive (PACAP-IR) fibers innervated adrenal chromaffin cells (often co-localized with choline acetyltransferase). Subcapsular fibers traversed the cortex-innervating endocrine cells and blood vessels [some co-storing mainly calcitonin gene-related peptide but also vasoactive intestinal polypeptide (VIP)].

View Article and Find Full Text PDF