Release of(14)C-adenine derivatives from cerebral cortical slices: Effect of phenytoin and phenobarbital.

Both ouabain, 0.1 mM, and veratridine, 0.05 mM, increased the release of(14)C-labeled compounds from rat cortical slices prelabeled with(14)C-adenine and incubated in vitro.

Polycystic kidney disease: earlier diagnosis using ultrasound.

A unique family study is presented, representing 124 members in four generations. Sixty-four patients were studied clinically, and 31 were found to have sonographic evidence of polycystic kidney disease. Findings on intravenous urography were positive in only 15 of these 31.

Ultrastructure of the venom gland of the scorpion, Centruroides sculpturatus (Ewing).

The venom apparatus of the scorpion, C. sculpturatus (Ewing) was studied with light and electron microscopy. Each of the paired glands is lined by secretory epithelium made up of a single layer of columnar cells.

Carbohydrate Accumulation and Metabolism in Escherichia coli: Characteristics of the Reversions of ctr Mutations.

The reversion behavior of pleiotropic carbohydrate mutants, previously designated as ctr, was studied. The mutants revert to complete restoration of the wild-type phenotype, as well as to a spectrum of partial wild-type phenotypes. Lac(+) reversions were found in the lac region (11 min) and some Mal(+) reversions occurred at malB (79 min), at a distance from the site of the ctr mutations (46 to 47 min).

Morphology of echovirus 22.

Purified preparations of echovirus 22 were examined in the electron microscope. The virus was found to possess 32 capsomers arranged at the vertices of either a pentakis dodecahedron or a rhombic triacontahedron. The size of the virions ranges from 22 x 10(-3) to 32 x 10(-3) mum with a mean of 27 x 10(-3) mum and a mode of 28 x 10(-3) mum.

An instrument for producing deep muscle relaxation by means of analog information feedback.

An instrument that assists subjects in attaining deep muscle relaxation by means of analog information feedback is described. Subjects hear a tone with a pitch proportional to the electromyographic activity in a given muscle group. Results showed that subjects receiving this type of analog feedback reached deeper levels of muscle relaxation than those receiving either no feedback or irrelevant feedback.

Inhibition of microorganisms by topical anesthetics.

The effect of various topical anesthetics and their preservatives on the growth of Pseudomonas aeruginosa, Staphyloccoccus albus, and Candida albicans was investigated. The topical anesthetics were proparacaine HCl, tetracaine HCl, cocaine HCl, and benoxinate HCl. The preservatives were chlorobutanol and butyl p-hydroxybenzoate.

THYMIDINE TRIPHOSPHATE SYNTHESIS IN TETRAHYMENA: I. Studies on Thymidine Kinase.

The amount of thymidine-H(3) converted to thymidine-H(3) monophosphate in 30 min formed the basis for assays of thymidine kinase in cell extracts from Tetrahymena pyriformis. The optimal concentration of adenosine triphosphate is lower than that required by other cell types. Thymidine triphosphate does not exercise any feedback control of the enzyme.

CHARACTERISTICS OF A STAPHYLOCOCCAL PHAGE CAPABLE OF TRANSDUCTION.

Morse, M. L. (University of Colorado Medical Center, Denver) and J.

GENETICS OF SOMATIC MAMMALIAN CELLS : I. DEMONSTRATION OF THE EXISTENCE OF MUTANTS WITH DIFFERENT GROWTH REQUIREMENTS IN A HUMAN CANCER CELL STRAIN (HELA).

The parental HeLa cell population, a morphologically uniform, human cancer cell strain, grown for several years in tissue culture by procedures always involving massive inocula, has been shown to contain different mutant cell types. Two clonal lines have been isolated and established as reliable stock cultures. Both strains exhibit 100 per cent plating efficiency in high or low serum concentrations in the presence of a feeder system.