Chromosome-Level Genome Assembly and Whole-Genome Resequencing Revealed Contrasting Population Genetic Differentiation of Black Bream () (Teleostei: Cyprinidae) Allopatric and Sympatric to Its Kin Species.

The black bream () is an economically important species widely distributed in China, with its geographic populations potentially having undergone differentiations and local adaptations. In this study, we presented a chromosome-level genome assembly of this species and investigated genetic differentiations of its populations that are allopatric (the northern one) and sympatric (the Poyang Lake) to its kin species, the blunt-snout bream (), using whole genome resequencing analysis. The results showed that the genome size of black bream was 1.

Deciphering the endogenous SUMO-1 landscape: a novel combinatorial peptide enrichment strategy for global profiling and disease association.

Small ubiquitin-like modifier (SUMO) plays a pivotal role in diverse cellular processes and is implicated in diseases such as cancer and neurodegenerative disorders. However, large-scale identification of endogenous SUMO-1 faces challenges due to limited enrichment methods and its lower abundance compared to SUMO-2/3. Here we propose a novel combinatorial peptide strategy, combined with anti-adhesive polymer development, to enrich endogenous SUMO-1 modified peptides, revealing a comprehensive SUMOylation landscape.

Causal Inference and Annotation of Phosphoproteomics Data in Multi-omics Cancer Studies.

Protein phosphorylation plays a crucial role in regulating diverse biological processes. Perturbations in protein phosphorylation are closely associated with downstream pathway dysfunctions, while alterations in protein expression could serve as sensitive indicators of pathological status. However, there are currently few methods that can accurately identify the regulatory links between protein phosphorylation and expression, given issues like reverse causation and confounders.

How Marine Megabenthos Fauna Responds to River Discharge and Artificial Flood in Large River Estuary.

Estuaries are ecologically sensitive areas influenced by river regulation. Knowledge of how marine megabenthos responds to river regulation and artificial flooding events remains limited. The study aims to provide a comprehensive understanding of the impacts of river regulation on marine megabenthic fauna.

Low-temperature oxidation of ethanol to acetaldehyde over Mo-based catalysts.

The research and development of the green synthesis route of chemicals has become the focus of research in academia and industry. At present, the highly efficient oxidation of ethanol to acetaldehyde over non-precious metal catalysts under mild conditions is most promising, but remains a big challenge. Herein, the Mo-Sn oxide catalyst was designed to successfully realize low-temperature oxidation of ethanol to acetaldehyde, achieving an acetaldehyde selectivity of 89.

Molecular engineering of supramolecular polymer adhesive with confined water and a single crown ether.

Here, we report a water-induced supramolecular polymer adhesive formed from confined water and an intrinsically amphiphilic macrocyclic self-assembly in a nanophase-separated structure. The selenium-containing crown ether macrocycle, featuring a strong hydrophilic hydrogen-bond receptor (selenoxide) and a synergistic hydrophobic selenium-substituted crown core, confines water within a segregated, interdigitated architecture. While water molecules typically freeze around 0 °C, the confined water in this supramolecular polymer remains in a liquid-like state down to -80 °C.

Phospholipase PlcH is involved in the secretion of cell wall glycoproteins and contributes to the host immune response of .

Glycosylphosphatidylinositol (GPI) anchoring is one of the conserved posttranslational modifications in eukaryotes that attach proteins to the plasma membrane. In fungi, in addition to plasma membrane GPI-anchored proteins (GPI-APs), some GPI-APs are specifically released from the cell membrane, secreted into the cell wall, and covalently linked to cell wall glucans as GPI-anchored cell wall proteins (GPI-CWPs). However, it remains unclear how fungal cells specifically release GPI-CWPs from their membranes.

GRAPE-WEB: An automated computational redesign web server for improving protein thermostability.

We have developed the GReedy Accumulated strategy for Protein Engineering (GRAPE) to improve enzyme stability across various applications, combining advanced computational methods with a unique clustering and greedy accumulation approach to efficiently explore epistatic effects with minimal experimental effort. To make this strategy accessible to nonexperts, we introduced GRAPE-WEB, an automated, user-friendly web server that allows the design, inspection, and combination of stabilizing mutations without requiring extensive bioinformatics knowledge. GRAPE-WEB's robust performance and accessibility provide a comprehensive and adaptable approach to protein thermostability design, suitable for both newcomers and experienced practitioners in the field.

Taxonomic notes of jumping spiders (Araneae, Salticidae) from Guangxi, Hainan, Sichuan, Xizang and Yunnan, China.

Twenty-one new species of jumping spiders from five provinces of South China are described: (♂), (♂♀), (♂♀), (♂♀), (♂♀), (♂), (♂♀), (♂♀), (♀), (♂♀), (♂♀), (♂♀), (♂♀), (♂♀), (♂♀), (♂♀), (♂), (♂♀), (♂♀), (♂♀), and (♂♀). Prószyński, 2016, is proposed as a junior synonym of Prószyński, 2016. Three new combinations are proposed: (Andreeva, Hęciak & Prószyński, 1984), and (Wanless, 1984), transferred from , and (Peng, Gong & Kim, 2000), transferred from Thorell, 1869.

Aromatic-aromatic interactions drive fold switch of GA95 and GB95 with three residue difference.

Proteins typically adopt a single fold to carry out their function, but metamorphic proteins, with multiple folding states, defy this norm. Deciphering the mechanism of conformational interconversion of metamorphic proteins is challenging. Herein, we employed nuclear magnetic resonance (NMR), circular dichroism (CD), and all-atom molecular dynamics (MD) simulations to elucidate the mechanism of fold switching in proteins GA95 and GB95, which share 95% sequence homology.

Determinants of regioselectivity of heterostructures in cation exchange reactions.

A cation exchange (CE) reaction offers a remarkable opportunity to create versatile metal sulfide nanocrystals (NCs) with arbitrary complexity in composition, structure, and functionality. The concept of regioselectivity has been discovered and developed to build the target heterostructures through CE reactions, yet a general principle of regioselectivity remains unclear. In this work, we establish connections between experimental results and theoretical insights to elucidate the determinants of regioselectivity using designed aliovalent CE reactions on a two-dimensional template.

Piezoelectric stimulation enhances bone regeneration in alveolar bone defects through metabolic reprogramming of macrophages.

Immunomodulation has emerged as a promising strategy for promoting bone regeneration. However, designing osteoimmunomodulatory biomaterial that can respond to mechanical stress in the unique microenvironment of alveolar bone under continuous occlusal stress remains a significant challenge. Herein, a wireless piezoelectric stimulation system, namely, piezoelectric hydrogel incorporating BaTiO nanoparticles (BTO NPs), is successfully developed to generate piezoelectric potentials for modulating macrophage reprogramming.

A perspective of lipid nanoparticles for RNA delivery.

Over the last two decades, lipid nanoparticles (LNPs) have evolved as an effective biocompatible and biodegradable RNA delivery platform in the fields of nanomedicine, biotechnology, and drug delivery. They are novel bionanomaterials that can be used to encapsulate a wide range of biomolecules, such as mRNA, as demonstrated by the current successes of COVID-19 mRNA vaccines. Therefore, it is important to provide a perspective on LNPs for RNA delivery, which further offers useful guidance for researchers who want to work in the RNA-based LNP field.

Discovery of a non-nucleotide stimulator of interferon genes (STING) agonist with systemic antitumor effect.

Agonists of the stimulator of interferon genes (STING) pathway are increasingly being recognized as a promising new approach in the treatment of cancer. Although progress in clinical trials for STING agonists in antitumor applications has been slow, there is still an urgent need for developing new potent STING agonists with versatile potential applications. Herein, we developed and identified a non-nucleotide STING agonist called DW18343.

First record of (Hemiptera, Scytinopteroidea, Scytinopteridae) from the Middle Triassic Tongchuan Entomofauna of China.

A new scytinopterid species, , is established based on a tegmen collected from the Middle Triassic Tongchuan Formation in Shaanxi Province, NW China. The new species can be easily separated from its congeners by the narrow tegminal apex, less curved terminal branches of stems RP, M and CuA and crossvein - connected to long vein M. This discovery represents the first record of from the Tongchuan Formation in China and suggests that the genus spread much more widely from Gondwana to northern Pangea in the Middle Triassic.

New insights into the mechanisms of red blood cell enucleation: From basics to clinical applications.

Background: Red blood cell (RBC) enucleation is a crucial step in the process of erythropoiesis. By removing the nucleus, RBCs gain greater flexibility, enabling them to traverse narrow capillaries with ease, thereby enhancing the efficiency of oxygen and carbon dioxide transport. This transformation underscores the intricate balance between cellular structure and function essential for maintaining homeostasis.

Perceptual Expertise of Forensic Examiners and Reviewers on Tests of Cross-Race and Disguised Face Identification and Face Memory.

Forensic facial professionals have been shown in previous studies to identify people from frontal face images more accurately than untrained participants when given 30 s per face pair. We tested whether this superiority holds in more challenging conditions. Two groups of forensic facial professionals (examiners, reviewers) and untrained participants were tested in three lab-based tasks: other-race face identification, disguised face identification, and face memory.

Evolutionary Genomics Provides Insights Into Endangerment and Conservation of a Wild Apple Tree Species, .

Understanding the evolutionary history of a species is essential for effective conservation management. , a relict broad-leaf forest tree found in arid Central Asian mountains, has a narrow and fragmented distribution and is classified as an endangered species in China. This species is considered one of the ancestors of the domesticated apple trees.

Seasonal Herbage-Livestock Balance and Grassland Pressure Index Analysis in the Yellow River Source Park, Tibet Plateau, China.

Grassland carrying capacity is an indicator for measuring the stability of grassland ecosystems and can provide a basis for formulating regional sustainable grazing strategies. However, most previous studies on this have only considered annual fluctuations, but seasonal changes were ignored. In this study, the herbage yield and nutrient value of two grassland types in Yellow River Source Park (YRSP) were measured by sampling point survey method in four seasons, and the seasonal and annual grassland carrying capacity, carrying numbers of standard sheep unit (SU) were estimated based on the dry matter (DM) content, crude protein, and metabolic energy of herbage.

Unusual Thermo-Enhanced Second Harmonic Generation in Organic Configurationally-Locked Polyene Crystals.

To modulate nonlinear optical (NLO) effects of crystalline material holds great application potential in the photoelectronic and optical fields. Organic configurationally-locked polyene represents an exciting NLO family with large second harmonic generation (SHG) effects, whereas it is a huge blank to switch and modulate their NLO property through external stimuli. For the first time, here present unusual thermo-enhanced SHG activities are presented in a polyene-based NLO compound, 2-{3-[2-(4-pyrrolidinphenyl)vinyl]-5,5-dimethylcyclohex-2-enylidene}malononitrile (1), giving a record-high magnitude of SHG enhancement up to ≈170% during its isomorphic phase transition.

Nitrogen regulation in polyester-based carbons and adsorption of gaseous benzene and ethyl acetate.

Nitrogen doping effectively improves the adsorption properties of activated carbons towards volatile organic compounds (VOCs); however, the role of nitrogen elements with various chemical valence states need further evaluation. In this work, waste polyester fabrics were used as a low-cost source to prepare activated carbon, and melamine, pyridine, dimethylamine, and pyrrole were selected as nitrogen sources to compare their nitrogen-doping ability. The adsorption of low-concentration benzene and ethyl acetate on the resultant carbons and the effects of nitrogen, including its valence states and contents, were investigated.

Noncentrosymmetric tellurite halides created by a depolymerization strategy: toward strong SHG intensity and wide bandgap.

The design and synthesis of nonlinear optical (NLO) materials with a strong second harmonic generation (SHG) effect and wide band gap is a long-standing challenge because of their inverse relationship, especially for metal tellurites. We found that a lower degree of polymerization of tellurite groups corresponds to a higher proportion of non-centrosymmetric (NCS) structures formed. Based on Pauling's rule #4, introduction of cations (Al/Ga) with higher coordination numbers and lower valence states than Te into the reaction system decreases the connectivity among tellurite groups.

Deep learning enabled ultra-high quality NMR chemical shift resolved spectra.

High quality chemical shift resolved spectra have long been pursued in nuclear magnetic resonance (NMR). In order to obtain chemical shift information with high resolution and sensitivity, a neural network named spin echo to obtain chemical shifts network (SE2CSNet) is developed to process the NMR data acquired by the spin echo pulse sequence. Through detecting the change of phase in the spin echo spectra, SE2CSNet can accurately detect the chemical shift position of spectral signals.